Acute Synovitis after Trauma Precedes and is Associated with Osteoarthritis Onset and Progression

Osteoarthritis (OA) is a whole-joint disease characterized by cartilage destruction, subchondral bone sclerosis, osteophyte formation, and synovitis. However, it remains unclear which part of the joint undergoes initial pathological changes that drives OA onset and progression. In the present study,...

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Veröffentlicht in:International journal of biological sciences 2020-01, Vol.16 (6), p.970-980
Hauptverfasser: Liao, Lifan, Zhang, Shanxing, Zhao, Lan, Chang, Xiaofeng, Han, Lin, Huang, Jian, Chen, Di
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creator Liao, Lifan
Zhang, Shanxing
Zhao, Lan
Chang, Xiaofeng
Han, Lin
Huang, Jian
Chen, Di
description Osteoarthritis (OA) is a whole-joint disease characterized by cartilage destruction, subchondral bone sclerosis, osteophyte formation, and synovitis. However, it remains unclear which part of the joint undergoes initial pathological changes that drives OA onset and progression. In the present study, we investigated the longitudinal alterations of the entire knee joint using a surgically-induced OA mouse model. Histology analysis showed that synovitis occurred as early as 1 week after destabilization of the medial meniscus (DMM), which preceded the events of cartilage degradation, subchondral sclerosis and osteophyte formation. Importantly, key pro-inflammatory cytokines such as IL-1β, IL-6, TNFα, and Ccl2, major matrix degrading enzymes including , and , as well as nerve growth factor (NGF), all increased significantly in both synovium and articular cartilage. It is notable that the inductions of these factors in synovium are far more extensive than those in articular cartilage. Results from behavioral tests demonstrated that sensitization of knee joint pain developed after 8 weeks, later than histological and molecular changes. In addition, the nanoindentation modulus of the medial tibiae decreased 4 weeks after DMM surgery, simultaneous with histological OA signs, which is also later than appearance of synovitis. Collectively, our data suggested that synovitis precedes and is associated with OA, and thus synovium may be an important target to intervene in OA treatment.
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However, it remains unclear which part of the joint undergoes initial pathological changes that drives OA onset and progression. In the present study, we investigated the longitudinal alterations of the entire knee joint using a surgically-induced OA mouse model. Histology analysis showed that synovitis occurred as early as 1 week after destabilization of the medial meniscus (DMM), which preceded the events of cartilage degradation, subchondral sclerosis and osteophyte formation. Importantly, key pro-inflammatory cytokines such as IL-1β, IL-6, TNFα, and Ccl2, major matrix degrading enzymes including , and , as well as nerve growth factor (NGF), all increased significantly in both synovium and articular cartilage. It is notable that the inductions of these factors in synovium are far more extensive than those in articular cartilage. Results from behavioral tests demonstrated that sensitization of knee joint pain developed after 8 weeks, later than histological and molecular changes. In addition, the nanoindentation modulus of the medial tibiae decreased 4 weeks after DMM surgery, simultaneous with histological OA signs, which is also later than appearance of synovitis. 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subjects Animals
Antigens
Arthralgia
Arthritis
Biomedical materials
Bone growth
Cartilage
Cartilage (articular)
Cartilage - pathology
Cartilage diseases
Collagenase 3
Cytokines
Destabilization
Gene expression
Growth factors
Histology
IL-1β
Immunohistochemistry
Inflammation
Interleukin 6
Joint diseases
Joints (anatomy)
Knee
Knee Joint - pathology
Laboratories
Mechanical properties
Meniscus
Mice
Mice, Inbred C57BL
Monocyte chemoattractant protein 1
Nanoindentation
Nerve growth factor
Osteoarthritis
Osteoarthritis - pathology
Osteoarthritis, Knee - pathology
Pain
Pathogenesis
Research Paper
Reverse Transcriptase Polymerase Chain Reaction
Sclerosis
Subchondral bone
Surgery
Synovitis
Synovitis - pathology
Synovium
Tibial Meniscus Injuries - pathology
Trauma
Tumor necrosis factor-α
Wounds and Injuries - pathology
X-Ray Microtomography
title Acute Synovitis after Trauma Precedes and is Associated with Osteoarthritis Onset and Progression
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