Impact of the used solvent on the reconstitution efficiency of evaporated biosamples for untargeted metabolomics studies
Introduction Untargeted metabolomics intends to objectively analyze a wide variety of compounds. Their diverse physicochemical properties make it difficult to choose an appropriate reconstitution solvent after sample evaporation without influencing the chromatography or hamper column sorbent integri...
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| Veröffentlicht in: | Metabolomics 2020-03, Vol.16 (3), p.34, Article 34 |
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| creator | Manier, Sascha K. Meyer, Markus R. |
| description | Introduction
Untargeted metabolomics intends to objectively analyze a wide variety of compounds. Their diverse physicochemical properties make it difficult to choose an appropriate reconstitution solvent after sample evaporation without influencing the chromatography or hamper column sorbent integrity.
Objectives
The study aimed to identify the most appropriate reconstitution solvent for blood plasma samples in terms of feature recovery, four endogenous compounds, and one selected internal standard.
Methods
We investigated several reconstitution solvent mixtures containing acetonitrile and methanol to resolve human plasma extract and evaluated them concerning the peak areas of tryptophan-d
5
, glucose, creatinine, palmitic acid, and the phophatidylcholine PC(P-16:0/P-16:0), as well as the total feature count
Results
Results indicated that acetonitrile containing 30% methanol was best suited to match all tested criteria at least for human blood plasma samples.
Conclusion
Despite identifying the mixture of acetonitrile and methanol being suitable as solvent for human blood plasma extracts, we recommend to systematically test for an appropriate reconstitution solvent for each analyzed biomatrix. |
| doi_str_mv | 10.1007/s11306-019-1631-1 |
| format | Article |
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Untargeted metabolomics intends to objectively analyze a wide variety of compounds. Their diverse physicochemical properties make it difficult to choose an appropriate reconstitution solvent after sample evaporation without influencing the chromatography or hamper column sorbent integrity.
Objectives
The study aimed to identify the most appropriate reconstitution solvent for blood plasma samples in terms of feature recovery, four endogenous compounds, and one selected internal standard.
Methods
We investigated several reconstitution solvent mixtures containing acetonitrile and methanol to resolve human plasma extract and evaluated them concerning the peak areas of tryptophan-d
5
, glucose, creatinine, palmitic acid, and the phophatidylcholine PC(P-16:0/P-16:0), as well as the total feature count
Results
Results indicated that acetonitrile containing 30% methanol was best suited to match all tested criteria at least for human blood plasma samples.
Conclusion
Despite identifying the mixture of acetonitrile and methanol being suitable as solvent for human blood plasma extracts, we recommend to systematically test for an appropriate reconstitution solvent for each analyzed biomatrix.</description><identifier>ISSN: 1573-3882</identifier><identifier>EISSN: 1573-3890</identifier><identifier>DOI: 10.1007/s11306-019-1631-1</identifier><identifier>PMID: 32124055</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Acetonitrile ; Acetonitriles - chemistry ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Blood ; Blood Glucose - metabolism ; Cell Biology ; Choline - analogs & derivatives ; Choline - blood ; Choline - metabolism ; Creatinine ; Creatinine - blood ; Creatinine - metabolism ; Developmental Biology ; Evaporation ; Life Sciences ; Metabolomics ; Methanol ; Methanol - chemistry ; Molecular Medicine ; Palmitic acid ; Palmitic Acid - blood ; Palmitic Acid - metabolism ; Physicochemical properties ; Plasma ; Short Communication ; Solvents ; Solvents - chemistry ; Tryptophan ; Tryptophan - blood ; Tryptophan - metabolism</subject><ispartof>Metabolomics, 2020-03, Vol.16 (3), p.34, Article 34</ispartof><rights>The Author(s) 2020</rights><rights>Metabolomics is a copyright of Springer, (2020). All Rights Reserved. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-959d052b322eaeca4fc4537e163d6a4ea9ac795ff20bf76fec4876ec6c8f98743</citedby><cites>FETCH-LOGICAL-c470t-959d052b322eaeca4fc4537e163d6a4ea9ac795ff20bf76fec4876ec6c8f98743</cites><orcidid>0000-0002-7126-5263 ; 0000-0003-4377-6784</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11306-019-1631-1$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11306-019-1631-1$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32124055$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Manier, Sascha K.</creatorcontrib><creatorcontrib>Meyer, Markus R.</creatorcontrib><title>Impact of the used solvent on the reconstitution efficiency of evaporated biosamples for untargeted metabolomics studies</title><title>Metabolomics</title><addtitle>Metabolomics</addtitle><addtitle>Metabolomics</addtitle><description>Introduction
Untargeted metabolomics intends to objectively analyze a wide variety of compounds. Their diverse physicochemical properties make it difficult to choose an appropriate reconstitution solvent after sample evaporation without influencing the chromatography or hamper column sorbent integrity.
Objectives
The study aimed to identify the most appropriate reconstitution solvent for blood plasma samples in terms of feature recovery, four endogenous compounds, and one selected internal standard.
Methods
We investigated several reconstitution solvent mixtures containing acetonitrile and methanol to resolve human plasma extract and evaluated them concerning the peak areas of tryptophan-d
5
, glucose, creatinine, palmitic acid, and the phophatidylcholine PC(P-16:0/P-16:0), as well as the total feature count
Results
Results indicated that acetonitrile containing 30% methanol was best suited to match all tested criteria at least for human blood plasma samples.
Conclusion
Despite identifying the mixture of acetonitrile and methanol being suitable as solvent for human blood plasma extracts, we recommend to systematically test for an appropriate reconstitution solvent for each analyzed biomatrix.</description><subject>Acetonitrile</subject><subject>Acetonitriles - chemistry</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blood</subject><subject>Blood Glucose - metabolism</subject><subject>Cell Biology</subject><subject>Choline - analogs & derivatives</subject><subject>Choline - blood</subject><subject>Choline - metabolism</subject><subject>Creatinine</subject><subject>Creatinine - blood</subject><subject>Creatinine - metabolism</subject><subject>Developmental Biology</subject><subject>Evaporation</subject><subject>Life Sciences</subject><subject>Metabolomics</subject><subject>Methanol</subject><subject>Methanol - chemistry</subject><subject>Molecular Medicine</subject><subject>Palmitic acid</subject><subject>Palmitic Acid - blood</subject><subject>Palmitic Acid - metabolism</subject><subject>Physicochemical properties</subject><subject>Plasma</subject><subject>Short Communication</subject><subject>Solvents</subject><subject>Solvents - chemistry</subject><subject>Tryptophan</subject><subject>Tryptophan - blood</subject><subject>Tryptophan - metabolism</subject><issn>1573-3882</issn><issn>1573-3890</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1UctO3DAUtaqiMgx8QDdVpK5D_UocbypVqAUkJDawthznejBK4tR2RuXvcQgd2gUrW_e87tVB6DPB5wRj8S0SwnBdYiJLUjNSkg9oQyrBStZI_PHwb-gxOonxEWPOpcCf0DGjhHJcVRv053qYtEmFt0V6gGKO0BXR93sY82x8mQUwfozJpTm5PAJrnXEwmqdFBHs9-aBTlrXORz1MPcTC-lDMY9JhBwsyQNKt7_3gTCximjsH8RQdWd1HOHt9t-j-18-7i6vy5vby-uLHTWm4wKmUlexwRVtGKWgwmlvDKyYg39vVmoOW2ghZWUtxa0VtwfBG1GBq01jZCM626PvqO83tAJ3JhwXdqym4QYcn5bVT_yOje1A7v1cix2LaZIOvrwbB_54hJvXo5zDmnRVltRSUNYxkFllZJvgYA9hDAsFqKUutZalcllrKUovmy7-rHRR_28kEuhJihsYdhLfo912fAaWWo8s</recordid><startdate>20200302</startdate><enddate>20200302</enddate><creator>Manier, Sascha K.</creator><creator>Meyer, Markus R.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7126-5263</orcidid><orcidid>https://orcid.org/0000-0003-4377-6784</orcidid></search><sort><creationdate>20200302</creationdate><title>Impact of the used solvent on the reconstitution efficiency of evaporated biosamples for untargeted metabolomics studies</title><author>Manier, Sascha K. ; Meyer, Markus R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-959d052b322eaeca4fc4537e163d6a4ea9ac795ff20bf76fec4876ec6c8f98743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acetonitrile</topic><topic>Acetonitriles - chemistry</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Blood</topic><topic>Blood Glucose - metabolism</topic><topic>Cell Biology</topic><topic>Choline - analogs & derivatives</topic><topic>Choline - blood</topic><topic>Choline - metabolism</topic><topic>Creatinine</topic><topic>Creatinine - blood</topic><topic>Creatinine - metabolism</topic><topic>Developmental Biology</topic><topic>Evaporation</topic><topic>Life Sciences</topic><topic>Metabolomics</topic><topic>Methanol</topic><topic>Methanol - chemistry</topic><topic>Molecular Medicine</topic><topic>Palmitic acid</topic><topic>Palmitic Acid - blood</topic><topic>Palmitic Acid - metabolism</topic><topic>Physicochemical properties</topic><topic>Plasma</topic><topic>Short Communication</topic><topic>Solvents</topic><topic>Solvents - chemistry</topic><topic>Tryptophan</topic><topic>Tryptophan - blood</topic><topic>Tryptophan - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Manier, Sascha K.</creatorcontrib><creatorcontrib>Meyer, Markus R.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Metabolomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Manier, Sascha K.</au><au>Meyer, Markus R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impact of the used solvent on the reconstitution efficiency of evaporated biosamples for untargeted metabolomics studies</atitle><jtitle>Metabolomics</jtitle><stitle>Metabolomics</stitle><addtitle>Metabolomics</addtitle><date>2020-03-02</date><risdate>2020</risdate><volume>16</volume><issue>3</issue><spage>34</spage><pages>34-</pages><artnum>34</artnum><issn>1573-3882</issn><eissn>1573-3890</eissn><abstract>Introduction
Untargeted metabolomics intends to objectively analyze a wide variety of compounds. Their diverse physicochemical properties make it difficult to choose an appropriate reconstitution solvent after sample evaporation without influencing the chromatography or hamper column sorbent integrity.
Objectives
The study aimed to identify the most appropriate reconstitution solvent for blood plasma samples in terms of feature recovery, four endogenous compounds, and one selected internal standard.
Methods
We investigated several reconstitution solvent mixtures containing acetonitrile and methanol to resolve human plasma extract and evaluated them concerning the peak areas of tryptophan-d
5
, glucose, creatinine, palmitic acid, and the phophatidylcholine PC(P-16:0/P-16:0), as well as the total feature count
Results
Results indicated that acetonitrile containing 30% methanol was best suited to match all tested criteria at least for human blood plasma samples.
Conclusion
Despite identifying the mixture of acetonitrile and methanol being suitable as solvent for human blood plasma extracts, we recommend to systematically test for an appropriate reconstitution solvent for each analyzed biomatrix.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>32124055</pmid><doi>10.1007/s11306-019-1631-1</doi><orcidid>https://orcid.org/0000-0002-7126-5263</orcidid><orcidid>https://orcid.org/0000-0003-4377-6784</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acetonitrile Acetonitriles - chemistry Biochemistry Biomedical and Life Sciences Biomedicine Blood Blood Glucose - metabolism Cell Biology Choline - analogs & derivatives Choline - blood Choline - metabolism Creatinine Creatinine - blood Creatinine - metabolism Developmental Biology Evaporation Life Sciences Metabolomics Methanol Methanol - chemistry Molecular Medicine Palmitic acid Palmitic Acid - blood Palmitic Acid - metabolism Physicochemical properties Plasma Short Communication Solvents Solvents - chemistry Tryptophan Tryptophan - blood Tryptophan - metabolism |
| title | Impact of the used solvent on the reconstitution efficiency of evaporated biosamples for untargeted metabolomics studies |
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