Carbon and nitrogen isotopic variability in foxtail millet (Setaria italica) with watering regime
Rationale Carbonised plant remains are analysed for reconstruction of past climates and agricultural regimes. Several recent studies have used C4 plants to address related questions, and correlations between modern C4 plant δ13C values and rainfall have been found. The millets were important food cr...
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| Veröffentlicht in: | Rapid communications in mass spectrometry 2020-03, Vol.34 (6), p.e8615-n/a |
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| creator | Lightfoot, Emma Ustunkaya, M. Cemre Przelomska, Natalia O'Connell, Tamsin C. Hunt, Harriet V. Jones, Martin K. Petrie, Cameron A. |
| description | Rationale
Carbonised plant remains are analysed for reconstruction of past climates and agricultural regimes. Several recent studies have used C4 plants to address related questions, and correlations between modern C4 plant δ13C values and rainfall have been found. The millets were important food crops in prehistoric Eurasia, yet little is known about causes of isotopic variation within millet species. Previous research has shown there to be significant isotopic variation between millet accessions. Here we compare isotope ratios from plants grown under different watering regimes. This allows for a consideration of whether or not Setaria italica is a good proxy for environmental reconstruction.
Methods
We compare stable isotope ratios of Setaria italica plants grown in a controlled environment chamber with different watering regimes. We compare the carbon isotope ratios of leaves and grains, and the nitrogen isotope ratios of grains, from 12 accessions of Setaria italica.
Results
We find significant isotopic variability between watering regimes. Carbon isotope ratios are positively correlated with water availability, and on average vary by 1.9‰ and 1.7‰ for leaves and grains, respectively. Grain nitrogen isotope ratios also vary with watering regime; however, the highest isotope ratios are found with the 130‐mL watering regime.
Conclusions
The carbon isotope ratios of Setaria italica are strongly correlated with water availability. However, the correlation is the opposite to that seen in studies of C3 plants. The difference in isotopic ratio due to watering regime is comparable with that seen between different accessions; thus distinguishing between changing varieties of Setaria italica and changing climate is problematic. In terms of grain nitrogen isotope ratios, the highest δ15N values were not associated with the lowest watering regime. Again, δ15N variation is comparable with that which would be expected from an aridity effect or a manuring effect, and thus distinguishing between these factors is probably problematic. |
| doi_str_mv | 10.1002/rcm.8615 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7050514</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2310301196</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4385-13b686dfee4be7c24e735972ef347c883eadec02a72b881c413b8a0c9759f24b3</originalsourceid><addsrcrecordid>eNp1kd9rFDEQx4Mo9qyCf4EEfGkftiabzW7yIpTDqlAR_PEcsrnZ65Rscia5nvffm7O1VMGneZjPfJiZLyEvOTvjjLVvkpvPVM_lI7LgTA8NawV_TBZMS950XKsj8izna8Y4ly17So4E76USSi-IXdo0xkBtWNGAJcU1BIo5lrhBR29sQjuix7KnGOgUfxaLns7oPRR68hXKAaBYrEdnT-kOyxXd2QIJw5omWOMMz8mTyfoML-7qMfl-8e7b8kNz-fn9x-X5ZeM6oWTDxdirfjUBdCMMru1gEFIPLUyiG5xSAuwKHGvt0I5KcdfVAWWZ04PUU9uN4pi8vfVutuMMKwehJOvNJuFs095Ei-bvTsArs443ZmCSSd5VwcmdIMUfW8jFzJgdeG8DxG029adM1BfqvqKv_0Gv4zaFel6lpNBC64dCl2LOCab7ZTgzh9xMzc0ccqvoq4fL34N_gqpAcwvs0MP-vyLzZfnpt_AXuoKiyA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2353939914</pqid></control><display><type>article</type><title>Carbon and nitrogen isotopic variability in foxtail millet (Setaria italica) with watering regime</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Lightfoot, Emma ; Ustunkaya, M. Cemre ; Przelomska, Natalia ; O'Connell, Tamsin C. ; Hunt, Harriet V. ; Jones, Martin K. ; Petrie, Cameron A.</creator><creatorcontrib>Lightfoot, Emma ; Ustunkaya, M. Cemre ; Przelomska, Natalia ; O'Connell, Tamsin C. ; Hunt, Harriet V. ; Jones, Martin K. ; Petrie, Cameron A.</creatorcontrib><description>Rationale
Carbonised plant remains are analysed for reconstruction of past climates and agricultural regimes. Several recent studies have used C4 plants to address related questions, and correlations between modern C4 plant δ13C values and rainfall have been found. The millets were important food crops in prehistoric Eurasia, yet little is known about causes of isotopic variation within millet species. Previous research has shown there to be significant isotopic variation between millet accessions. Here we compare isotope ratios from plants grown under different watering regimes. This allows for a consideration of whether or not Setaria italica is a good proxy for environmental reconstruction.
Methods
We compare stable isotope ratios of Setaria italica plants grown in a controlled environment chamber with different watering regimes. We compare the carbon isotope ratios of leaves and grains, and the nitrogen isotope ratios of grains, from 12 accessions of Setaria italica.
Results
We find significant isotopic variability between watering regimes. Carbon isotope ratios are positively correlated with water availability, and on average vary by 1.9‰ and 1.7‰ for leaves and grains, respectively. Grain nitrogen isotope ratios also vary with watering regime; however, the highest isotope ratios are found with the 130‐mL watering regime.
Conclusions
The carbon isotope ratios of Setaria italica are strongly correlated with water availability. However, the correlation is the opposite to that seen in studies of C3 plants. The difference in isotopic ratio due to watering regime is comparable with that seen between different accessions; thus distinguishing between changing varieties of Setaria italica and changing climate is problematic. In terms of grain nitrogen isotope ratios, the highest δ15N values were not associated with the lowest watering regime. Again, δ15N variation is comparable with that which would be expected from an aridity effect or a manuring effect, and thus distinguishing between these factors is probably problematic.</description><identifier>ISSN: 0951-4198</identifier><identifier>EISSN: 1097-0231</identifier><identifier>DOI: 10.1002/rcm.8615</identifier><identifier>PMID: 31658389</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Aridity ; Carbon ; Carbon isotopes ; Carbon Isotopes - analysis ; Carbon Isotopes - metabolism ; Crops, Agricultural - chemistry ; Crops, Agricultural - growth & development ; Crops, Agricultural - metabolism ; Edible Grain - chemistry ; Edible Grain - growth & development ; Edible Grain - metabolism ; Grain ; Isotope ratios ; Isotopes ; Millet ; Nitrogen ; Nitrogen isotopes ; Nitrogen Isotopes - analysis ; Nitrogen Isotopes - metabolism ; Photosynthesis ; Plant Leaves - chemistry ; Plant Leaves - growth & development ; Plant Leaves - metabolism ; Rainfall ; Reconstruction ; Setaria Plant - chemistry ; Setaria Plant - growth & development ; Setaria Plant - metabolism ; Variability ; Water - metabolism</subject><ispartof>Rapid communications in mass spectrometry, 2020-03, Vol.34 (6), p.e8615-n/a</ispartof><rights>2019 The Authors. Rapid Communications in Mass Spectrometry published by John Wiley & Sons Ltd</rights><rights>2019 The Authors. Rapid Communications in Mass Spectrometry published by John Wiley & Sons Ltd.</rights><rights>2020 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4385-13b686dfee4be7c24e735972ef347c883eadec02a72b881c413b8a0c9759f24b3</citedby><cites>FETCH-LOGICAL-c4385-13b686dfee4be7c24e735972ef347c883eadec02a72b881c413b8a0c9759f24b3</cites><orcidid>0000-0002-2926-7230 ; 0000-0002-4744-0332 ; 0000-0003-4217-1695 ; 0000-0002-0823-4720 ; 0000-0001-9207-4565</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Frcm.8615$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Frcm.8615$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,1417,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31658389$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lightfoot, Emma</creatorcontrib><creatorcontrib>Ustunkaya, M. Cemre</creatorcontrib><creatorcontrib>Przelomska, Natalia</creatorcontrib><creatorcontrib>O'Connell, Tamsin C.</creatorcontrib><creatorcontrib>Hunt, Harriet V.</creatorcontrib><creatorcontrib>Jones, Martin K.</creatorcontrib><creatorcontrib>Petrie, Cameron A.</creatorcontrib><title>Carbon and nitrogen isotopic variability in foxtail millet (Setaria italica) with watering regime</title><title>Rapid communications in mass spectrometry</title><addtitle>Rapid Commun Mass Spectrom</addtitle><description>Rationale
Carbonised plant remains are analysed for reconstruction of past climates and agricultural regimes. Several recent studies have used C4 plants to address related questions, and correlations between modern C4 plant δ13C values and rainfall have been found. The millets were important food crops in prehistoric Eurasia, yet little is known about causes of isotopic variation within millet species. Previous research has shown there to be significant isotopic variation between millet accessions. Here we compare isotope ratios from plants grown under different watering regimes. This allows for a consideration of whether or not Setaria italica is a good proxy for environmental reconstruction.
Methods
We compare stable isotope ratios of Setaria italica plants grown in a controlled environment chamber with different watering regimes. We compare the carbon isotope ratios of leaves and grains, and the nitrogen isotope ratios of grains, from 12 accessions of Setaria italica.
Results
We find significant isotopic variability between watering regimes. Carbon isotope ratios are positively correlated with water availability, and on average vary by 1.9‰ and 1.7‰ for leaves and grains, respectively. Grain nitrogen isotope ratios also vary with watering regime; however, the highest isotope ratios are found with the 130‐mL watering regime.
Conclusions
The carbon isotope ratios of Setaria italica are strongly correlated with water availability. However, the correlation is the opposite to that seen in studies of C3 plants. The difference in isotopic ratio due to watering regime is comparable with that seen between different accessions; thus distinguishing between changing varieties of Setaria italica and changing climate is problematic. In terms of grain nitrogen isotope ratios, the highest δ15N values were not associated with the lowest watering regime. Again, δ15N variation is comparable with that which would be expected from an aridity effect or a manuring effect, and thus distinguishing between these factors is probably problematic.</description><subject>Aridity</subject><subject>Carbon</subject><subject>Carbon isotopes</subject><subject>Carbon Isotopes - analysis</subject><subject>Carbon Isotopes - metabolism</subject><subject>Crops, Agricultural - chemistry</subject><subject>Crops, Agricultural - growth & development</subject><subject>Crops, Agricultural - metabolism</subject><subject>Edible Grain - chemistry</subject><subject>Edible Grain - growth & development</subject><subject>Edible Grain - metabolism</subject><subject>Grain</subject><subject>Isotope ratios</subject><subject>Isotopes</subject><subject>Millet</subject><subject>Nitrogen</subject><subject>Nitrogen isotopes</subject><subject>Nitrogen Isotopes - analysis</subject><subject>Nitrogen Isotopes - metabolism</subject><subject>Photosynthesis</subject><subject>Plant Leaves - chemistry</subject><subject>Plant Leaves - growth & development</subject><subject>Plant Leaves - metabolism</subject><subject>Rainfall</subject><subject>Reconstruction</subject><subject>Setaria Plant - chemistry</subject><subject>Setaria Plant - growth & development</subject><subject>Setaria Plant - metabolism</subject><subject>Variability</subject><subject>Water - metabolism</subject><issn>0951-4198</issn><issn>1097-0231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNp1kd9rFDEQx4Mo9qyCf4EEfGkftiabzW7yIpTDqlAR_PEcsrnZ65Rscia5nvffm7O1VMGneZjPfJiZLyEvOTvjjLVvkpvPVM_lI7LgTA8NawV_TBZMS950XKsj8izna8Y4ly17So4E76USSi-IXdo0xkBtWNGAJcU1BIo5lrhBR29sQjuix7KnGOgUfxaLns7oPRR68hXKAaBYrEdnT-kOyxXd2QIJw5omWOMMz8mTyfoML-7qMfl-8e7b8kNz-fn9x-X5ZeM6oWTDxdirfjUBdCMMru1gEFIPLUyiG5xSAuwKHGvt0I5KcdfVAWWZ04PUU9uN4pi8vfVutuMMKwehJOvNJuFs095Ei-bvTsArs443ZmCSSd5VwcmdIMUfW8jFzJgdeG8DxG029adM1BfqvqKv_0Gv4zaFel6lpNBC64dCl2LOCab7ZTgzh9xMzc0ccqvoq4fL34N_gqpAcwvs0MP-vyLzZfnpt_AXuoKiyA</recordid><startdate>20200330</startdate><enddate>20200330</enddate><creator>Lightfoot, Emma</creator><creator>Ustunkaya, M. Cemre</creator><creator>Przelomska, Natalia</creator><creator>O'Connell, Tamsin C.</creator><creator>Hunt, Harriet V.</creator><creator>Jones, Martin K.</creator><creator>Petrie, Cameron A.</creator><general>Wiley Subscription Services, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>JQ2</scope><scope>L7M</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-2926-7230</orcidid><orcidid>https://orcid.org/0000-0002-4744-0332</orcidid><orcidid>https://orcid.org/0000-0003-4217-1695</orcidid><orcidid>https://orcid.org/0000-0002-0823-4720</orcidid><orcidid>https://orcid.org/0000-0001-9207-4565</orcidid></search><sort><creationdate>20200330</creationdate><title>Carbon and nitrogen isotopic variability in foxtail millet (Setaria italica) with watering regime</title><author>Lightfoot, Emma ; Ustunkaya, M. Cemre ; Przelomska, Natalia ; O'Connell, Tamsin C. ; Hunt, Harriet V. ; Jones, Martin K. ; Petrie, Cameron A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4385-13b686dfee4be7c24e735972ef347c883eadec02a72b881c413b8a0c9759f24b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Aridity</topic><topic>Carbon</topic><topic>Carbon isotopes</topic><topic>Carbon Isotopes - analysis</topic><topic>Carbon Isotopes - metabolism</topic><topic>Crops, Agricultural - chemistry</topic><topic>Crops, Agricultural - growth & development</topic><topic>Crops, Agricultural - metabolism</topic><topic>Edible Grain - chemistry</topic><topic>Edible Grain - growth & development</topic><topic>Edible Grain - metabolism</topic><topic>Grain</topic><topic>Isotope ratios</topic><topic>Isotopes</topic><topic>Millet</topic><topic>Nitrogen</topic><topic>Nitrogen isotopes</topic><topic>Nitrogen Isotopes - analysis</topic><topic>Nitrogen Isotopes - metabolism</topic><topic>Photosynthesis</topic><topic>Plant Leaves - chemistry</topic><topic>Plant Leaves - growth & development</topic><topic>Plant Leaves - metabolism</topic><topic>Rainfall</topic><topic>Reconstruction</topic><topic>Setaria Plant - chemistry</topic><topic>Setaria Plant - growth & development</topic><topic>Setaria Plant - metabolism</topic><topic>Variability</topic><topic>Water - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lightfoot, Emma</creatorcontrib><creatorcontrib>Ustunkaya, M. Cemre</creatorcontrib><creatorcontrib>Przelomska, Natalia</creatorcontrib><creatorcontrib>O'Connell, Tamsin C.</creatorcontrib><creatorcontrib>Hunt, Harriet V.</creatorcontrib><creatorcontrib>Jones, Martin K.</creatorcontrib><creatorcontrib>Petrie, Cameron A.</creatorcontrib><collection>Wiley-Blackwell Open Access Titles</collection><collection>Wiley Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Rapid communications in mass spectrometry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lightfoot, Emma</au><au>Ustunkaya, M. Cemre</au><au>Przelomska, Natalia</au><au>O'Connell, Tamsin C.</au><au>Hunt, Harriet V.</au><au>Jones, Martin K.</au><au>Petrie, Cameron A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Carbon and nitrogen isotopic variability in foxtail millet (Setaria italica) with watering regime</atitle><jtitle>Rapid communications in mass spectrometry</jtitle><addtitle>Rapid Commun Mass Spectrom</addtitle><date>2020-03-30</date><risdate>2020</risdate><volume>34</volume><issue>6</issue><spage>e8615</spage><epage>n/a</epage><pages>e8615-n/a</pages><issn>0951-4198</issn><eissn>1097-0231</eissn><abstract>Rationale
Carbonised plant remains are analysed for reconstruction of past climates and agricultural regimes. Several recent studies have used C4 plants to address related questions, and correlations between modern C4 plant δ13C values and rainfall have been found. The millets were important food crops in prehistoric Eurasia, yet little is known about causes of isotopic variation within millet species. Previous research has shown there to be significant isotopic variation between millet accessions. Here we compare isotope ratios from plants grown under different watering regimes. This allows for a consideration of whether or not Setaria italica is a good proxy for environmental reconstruction.
Methods
We compare stable isotope ratios of Setaria italica plants grown in a controlled environment chamber with different watering regimes. We compare the carbon isotope ratios of leaves and grains, and the nitrogen isotope ratios of grains, from 12 accessions of Setaria italica.
Results
We find significant isotopic variability between watering regimes. Carbon isotope ratios are positively correlated with water availability, and on average vary by 1.9‰ and 1.7‰ for leaves and grains, respectively. Grain nitrogen isotope ratios also vary with watering regime; however, the highest isotope ratios are found with the 130‐mL watering regime.
Conclusions
The carbon isotope ratios of Setaria italica are strongly correlated with water availability. However, the correlation is the opposite to that seen in studies of C3 plants. The difference in isotopic ratio due to watering regime is comparable with that seen between different accessions; thus distinguishing between changing varieties of Setaria italica and changing climate is problematic. In terms of grain nitrogen isotope ratios, the highest δ15N values were not associated with the lowest watering regime. Again, δ15N variation is comparable with that which would be expected from an aridity effect or a manuring effect, and thus distinguishing between these factors is probably problematic.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31658389</pmid><doi>10.1002/rcm.8615</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-2926-7230</orcidid><orcidid>https://orcid.org/0000-0002-4744-0332</orcidid><orcidid>https://orcid.org/0000-0003-4217-1695</orcidid><orcidid>https://orcid.org/0000-0002-0823-4720</orcidid><orcidid>https://orcid.org/0000-0001-9207-4565</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Aridity Carbon Carbon isotopes Carbon Isotopes - analysis Carbon Isotopes - metabolism Crops, Agricultural - chemistry Crops, Agricultural - growth & development Crops, Agricultural - metabolism Edible Grain - chemistry Edible Grain - growth & development Edible Grain - metabolism Grain Isotope ratios Isotopes Millet Nitrogen Nitrogen isotopes Nitrogen Isotopes - analysis Nitrogen Isotopes - metabolism Photosynthesis Plant Leaves - chemistry Plant Leaves - growth & development Plant Leaves - metabolism Rainfall Reconstruction Setaria Plant - chemistry Setaria Plant - growth & development Setaria Plant - metabolism Variability Water - metabolism |
| title | Carbon and nitrogen isotopic variability in foxtail millet (Setaria italica) with watering regime |
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