A Photo‐Crosslinkable Kidney ECM‐Derived Bioink Accelerates Renal Tissue Formation
3D bioprinting strategies in tissue engineering aim to fabricate clinically applicable tissue constructs that can replace the damaged or diseased tissues and organs. One of the main prerequisites in 3D bioprinting is finding an appropriate bioink that provides a tissue‐specific microenvironment supp...
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| Veröffentlicht in: | Advanced healthcare materials 2019-04, Vol.8 (7), p.e1800992-n/a |
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| creator | Ali, Mohamed PR, Anil Kumar Yoo, James J. Zahran, Faten Atala, Anthony Lee, Sang Jin |
| description | 3D bioprinting strategies in tissue engineering aim to fabricate clinically applicable tissue constructs that can replace the damaged or diseased tissues and organs. One of the main prerequisites in 3D bioprinting is finding an appropriate bioink that provides a tissue‐specific microenvironment supporting the cellular growth and maturation. In this respect, decellularized extracellular matrix (dECM)‐derived hydrogels have been considered as bioinks for the cell‐based bioprinting due to their capability to inherit the intrinsic cues from native ECM. Herein, a photo‐crosslinkable kidney ECM‐derived bioink (KdECMMA) is developed that could provide a kidney‐specific microenvironment for renal tissue bioprinting. Porcine whole kidneys are decellularized through a perfusion method, dissolved in an acid solution, and chemically modified by methacrylation. A KdECMMA‐based bioink is formulated and evaluated for rheological properties and printability for the printing process. The results show that the bioprinted human kidney cells in the KdECMMA bioink are highly viable and mature with time. Moreover, the bioprinted renal constructs exhibit the structural and functional characteristics of the native renal tissue. The potential of the tissue‐specific ECM‐derived bioink is demonstrated for cell‐based bioprinting that could enhance the cellular maturation and eventually tissue formation.
This study aims to develop a novel photo‐crosslinkable kidney‐specific extracellular matrix (ECM)‐based bioink formulation that can support the human kidney cell maturation and tissue formation in the printed construct. 3D bioprinting strategy with this novel kidney‐specific ECM bioink has great potential to bioengineer a functional renal tissue construct for use in future regenerative medicine applications. |
| doi_str_mv | 10.1002/adhm.201800992 |
| format | Article |
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This study aims to develop a novel photo‐crosslinkable kidney‐specific extracellular matrix (ECM)‐based bioink formulation that can support the human kidney cell maturation and tissue formation in the printed construct. 3D bioprinting strategy with this novel kidney‐specific ECM bioink has great potential to bioengineer a functional renal tissue construct for use in future regenerative medicine applications.</description><identifier>ISSN: 2192-2640</identifier><identifier>EISSN: 2192-2659</identifier><identifier>DOI: 10.1002/adhm.201800992</identifier><identifier>PMID: 30725520</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Bioengineering ; bioinks ; bioprinting ; Crosslinking ; decellularization ; extracellular matrices ; Extracellular matrix ; Hydrogels ; Kidneys ; Maturation ; Organic chemistry ; Organs ; Perfusion ; Rheological properties ; Structure-function relationships ; Three dimensional printing ; Tissue engineering</subject><ispartof>Advanced healthcare materials, 2019-04, Vol.8 (7), p.e1800992-n/a</ispartof><rights>2019 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5972-1322c480562af5ce91062909c2153f5cc769adb77fb0e8b642f33d625d0da1fe3</citedby><cites>FETCH-LOGICAL-c5972-1322c480562af5ce91062909c2153f5cc769adb77fb0e8b642f33d625d0da1fe3</cites><orcidid>0000-0002-3899-1909 ; 0000-0003-1610-7323 ; 0000-0002-7827-3848</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fadhm.201800992$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fadhm.201800992$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30725520$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ali, Mohamed</creatorcontrib><creatorcontrib>PR, Anil Kumar</creatorcontrib><creatorcontrib>Yoo, James J.</creatorcontrib><creatorcontrib>Zahran, Faten</creatorcontrib><creatorcontrib>Atala, Anthony</creatorcontrib><creatorcontrib>Lee, Sang Jin</creatorcontrib><title>A Photo‐Crosslinkable Kidney ECM‐Derived Bioink Accelerates Renal Tissue Formation</title><title>Advanced healthcare materials</title><addtitle>Adv Healthc Mater</addtitle><description>3D bioprinting strategies in tissue engineering aim to fabricate clinically applicable tissue constructs that can replace the damaged or diseased tissues and organs. One of the main prerequisites in 3D bioprinting is finding an appropriate bioink that provides a tissue‐specific microenvironment supporting the cellular growth and maturation. In this respect, decellularized extracellular matrix (dECM)‐derived hydrogels have been considered as bioinks for the cell‐based bioprinting due to their capability to inherit the intrinsic cues from native ECM. Herein, a photo‐crosslinkable kidney ECM‐derived bioink (KdECMMA) is developed that could provide a kidney‐specific microenvironment for renal tissue bioprinting. Porcine whole kidneys are decellularized through a perfusion method, dissolved in an acid solution, and chemically modified by methacrylation. A KdECMMA‐based bioink is formulated and evaluated for rheological properties and printability for the printing process. The results show that the bioprinted human kidney cells in the KdECMMA bioink are highly viable and mature with time. Moreover, the bioprinted renal constructs exhibit the structural and functional characteristics of the native renal tissue. The potential of the tissue‐specific ECM‐derived bioink is demonstrated for cell‐based bioprinting that could enhance the cellular maturation and eventually tissue formation.
This study aims to develop a novel photo‐crosslinkable kidney‐specific extracellular matrix (ECM)‐based bioink formulation that can support the human kidney cell maturation and tissue formation in the printed construct. 3D bioprinting strategy with this novel kidney‐specific ECM bioink has great potential to bioengineer a functional renal tissue construct for use in future regenerative medicine applications.</description><subject>Bioengineering</subject><subject>bioinks</subject><subject>bioprinting</subject><subject>Crosslinking</subject><subject>decellularization</subject><subject>extracellular matrices</subject><subject>Extracellular matrix</subject><subject>Hydrogels</subject><subject>Kidneys</subject><subject>Maturation</subject><subject>Organic chemistry</subject><subject>Organs</subject><subject>Perfusion</subject><subject>Rheological properties</subject><subject>Structure-function relationships</subject><subject>Three dimensional printing</subject><subject>Tissue engineering</subject><issn>2192-2640</issn><issn>2192-2659</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNqFkc1uEzEUhS0EolXoliUaiQ2bhOvr2BNvkELaUkQrECpsLY_nDnGZGbf2TFF2PALPyJPgkBJ-Nnhjy-fz0T0-jD3mMOMA-NzW626GwBcAWuM9dohc4xSV1Pf35zkcsKOUriAvJbla8IfsQECJUiIcso_L4t06DOH712-rGFJqff_ZVi0Vb3zd06Y4WV1k6Ziiv6W6eOlD1oulc9RStAOl4j31ti0ufUojFachdnbwoX_EHjS2TXR0t0_Yh9OTy9XZ9Pztq9er5fnUSV3ilAtEN1-AVGgb6UhzUKhBO-RS5AtXKm3rqiybCmhRqTk2QtQKZQ215Q2JCXux870eq45qR_0QbWuuo-9s3Jhgvflb6f3afAq3pgShpZDZ4NmdQQw3I6XBdD7ldK3tKYzJIELJlRCZn7Cn_6BXYYw5_U8qD6WAbw1nO8ptvzNSsx-Gg9m2ZratmX1r-cGTPyPs8V8dZUDvgC--pc1_7Mzy-Ozit_kPKGalAQ</recordid><startdate>201904</startdate><enddate>201904</enddate><creator>Ali, Mohamed</creator><creator>PR, Anil Kumar</creator><creator>Yoo, James J.</creator><creator>Zahran, Faten</creator><creator>Atala, Anthony</creator><creator>Lee, Sang Jin</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T5</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7TO</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-3899-1909</orcidid><orcidid>https://orcid.org/0000-0003-1610-7323</orcidid><orcidid>https://orcid.org/0000-0002-7827-3848</orcidid></search><sort><creationdate>201904</creationdate><title>A Photo‐Crosslinkable Kidney ECM‐Derived Bioink Accelerates Renal Tissue Formation</title><author>Ali, Mohamed ; PR, Anil Kumar ; Yoo, James J. ; Zahran, Faten ; Atala, Anthony ; Lee, Sang Jin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5972-1322c480562af5ce91062909c2153f5cc769adb77fb0e8b642f33d625d0da1fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Bioengineering</topic><topic>bioinks</topic><topic>bioprinting</topic><topic>Crosslinking</topic><topic>decellularization</topic><topic>extracellular matrices</topic><topic>Extracellular matrix</topic><topic>Hydrogels</topic><topic>Kidneys</topic><topic>Maturation</topic><topic>Organic chemistry</topic><topic>Organs</topic><topic>Perfusion</topic><topic>Rheological properties</topic><topic>Structure-function relationships</topic><topic>Three dimensional printing</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ali, Mohamed</creatorcontrib><creatorcontrib>PR, Anil Kumar</creatorcontrib><creatorcontrib>Yoo, James J.</creatorcontrib><creatorcontrib>Zahran, Faten</creatorcontrib><creatorcontrib>Atala, Anthony</creatorcontrib><creatorcontrib>Lee, Sang Jin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Immunology Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Advanced healthcare materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ali, Mohamed</au><au>PR, Anil Kumar</au><au>Yoo, James J.</au><au>Zahran, Faten</au><au>Atala, Anthony</au><au>Lee, Sang Jin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Photo‐Crosslinkable Kidney ECM‐Derived Bioink Accelerates Renal Tissue Formation</atitle><jtitle>Advanced healthcare materials</jtitle><addtitle>Adv Healthc Mater</addtitle><date>2019-04</date><risdate>2019</risdate><volume>8</volume><issue>7</issue><spage>e1800992</spage><epage>n/a</epage><pages>e1800992-n/a</pages><issn>2192-2640</issn><eissn>2192-2659</eissn><abstract>3D bioprinting strategies in tissue engineering aim to fabricate clinically applicable tissue constructs that can replace the damaged or diseased tissues and organs. One of the main prerequisites in 3D bioprinting is finding an appropriate bioink that provides a tissue‐specific microenvironment supporting the cellular growth and maturation. In this respect, decellularized extracellular matrix (dECM)‐derived hydrogels have been considered as bioinks for the cell‐based bioprinting due to their capability to inherit the intrinsic cues from native ECM. Herein, a photo‐crosslinkable kidney ECM‐derived bioink (KdECMMA) is developed that could provide a kidney‐specific microenvironment for renal tissue bioprinting. Porcine whole kidneys are decellularized through a perfusion method, dissolved in an acid solution, and chemically modified by methacrylation. A KdECMMA‐based bioink is formulated and evaluated for rheological properties and printability for the printing process. The results show that the bioprinted human kidney cells in the KdECMMA bioink are highly viable and mature with time. Moreover, the bioprinted renal constructs exhibit the structural and functional characteristics of the native renal tissue. The potential of the tissue‐specific ECM‐derived bioink is demonstrated for cell‐based bioprinting that could enhance the cellular maturation and eventually tissue formation.
This study aims to develop a novel photo‐crosslinkable kidney‐specific extracellular matrix (ECM)‐based bioink formulation that can support the human kidney cell maturation and tissue formation in the printed construct. 3D bioprinting strategy with this novel kidney‐specific ECM bioink has great potential to bioengineer a functional renal tissue construct for use in future regenerative medicine applications.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30725520</pmid><doi>10.1002/adhm.201800992</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-3899-1909</orcidid><orcidid>https://orcid.org/0000-0003-1610-7323</orcidid><orcidid>https://orcid.org/0000-0002-7827-3848</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Advanced healthcare materials, 2019-04, Vol.8 (7), p.e1800992-n/a |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Bioengineering bioinks bioprinting Crosslinking decellularization extracellular matrices Extracellular matrix Hydrogels Kidneys Maturation Organic chemistry Organs Perfusion Rheological properties Structure-function relationships Three dimensional printing Tissue engineering |
| title | A Photo‐Crosslinkable Kidney ECM‐Derived Bioink Accelerates Renal Tissue Formation |
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