Overexpression of HURP mRNA in head and neck carcinoma and association with in vitro response to vinorelbine
gene encodes the hepatoma upregulated protein (HURP), a microtubule associated protein regulating mitotic spindle dynamics, which promotes chromosomal congression and alignment during mitosis, with a potential role in tumorigenesis. In the present study, mRNA expression was investigated by reverse t...
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| Veröffentlicht in: | Oncology letters 2020-03, Vol.19 (3), p.2502-2507 |
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| creator | Al-Khafaji, Ahmed S K Pantazi, Paschalia Acha-Sagredo, Amelia Schache, Andrew Risk, Janet M Shaw, Richard J Liloglou, Triantafillos |
| description | gene encodes the hepatoma upregulated protein (HURP), a microtubule associated protein regulating mitotic spindle dynamics, which promotes chromosomal congression and alignment during mitosis, with a potential role in tumorigenesis. In the present study,
mRNA expression was investigated by reverse transcription-quantitative PCR in oropharyngeal squamous cell carcinoma (OPSCC). Primary OPSCC tumors from 107 patients and 48 adjacent normal tissues, as well as 12 respiratory tract cancer cell lines (9 head and neck squamous cell carcinoma, 2 lung cancer and 1 normal bronchial) were utilised in the present study. mRNA expression levels of
were higher in malignant OPSCC tissues compared with in normal mucosa (P |
| doi_str_mv | 10.3892/ol.2020.11339 |
| format | Article |
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mRNA expression was investigated by reverse transcription-quantitative PCR in oropharyngeal squamous cell carcinoma (OPSCC). Primary OPSCC tumors from 107 patients and 48 adjacent normal tissues, as well as 12 respiratory tract cancer cell lines (9 head and neck squamous cell carcinoma, 2 lung cancer and 1 normal bronchial) were utilised in the present study. mRNA expression levels of
were higher in malignant OPSCC tissues compared with in normal mucosa (P<1×10
) and significantly associated with sex and smoking status (P<0.0001). Vinorelbine
toxicity at half-maximal inhibitory concentration (IC
) was measured in the 11 cancer cell lines using an MTT assay. Sensitivity to vinorelbine was significantly correlated with HURP expression (r=0.636; P=0.035). The data indicated that
overexpression is frequent in OPSCC tissues and associated with smoking. The correlation between
mRNA expression and vinorelbine
response suggests that HURP is a potential modulator of vinorelbine response; therefore, it should be explored for its possible predictive value for the efficiency of vinorelbine treatment in this type of cancer.</description><identifier>ISSN: 1792-1074</identifier><identifier>EISSN: 1792-1082</identifier><identifier>DOI: 10.3892/ol.2020.11339</identifier><identifier>PMID: 32194751</identifier><language>eng</language><publisher>Greece: Spandidos Publications</publisher><subject>Alcohol ; Biobanks ; Cancer ; Cancer research ; Carcinoma ; Cell division ; Chromosomes ; Gender ; Gene expression ; Genes ; Head & neck cancer ; Hepatocellular carcinoma ; Human papillomavirus ; Liver cancer ; Lung cancer ; Lymphatic system ; Messenger RNA ; Oncology ; Papillomavirus ; Papillomavirus infections ; Polymerase chain reaction ; Proteins ; Respiratory tract cancer ; RNA ; Scientific equipment industry ; Smoking ; Software ; Squamous cell carcinoma ; Studies ; Throat cancer ; Tobacco ; Toxicity ; Tumorigenesis ; Tumors ; Values ; Vinorelbine</subject><ispartof>Oncology letters, 2020-03, Vol.19 (3), p.2502-2507</ispartof><rights>Copyright © 2020, Spandidos Publications.</rights><rights>COPYRIGHT 2020 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2020</rights><rights>Copyright © 2020, Spandidos Publications 2020</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039110/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039110/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32194751$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Al-Khafaji, Ahmed S K</creatorcontrib><creatorcontrib>Pantazi, Paschalia</creatorcontrib><creatorcontrib>Acha-Sagredo, Amelia</creatorcontrib><creatorcontrib>Schache, Andrew</creatorcontrib><creatorcontrib>Risk, Janet M</creatorcontrib><creatorcontrib>Shaw, Richard J</creatorcontrib><creatorcontrib>Liloglou, Triantafillos</creatorcontrib><title>Overexpression of HURP mRNA in head and neck carcinoma and association with in vitro response to vinorelbine</title><title>Oncology letters</title><addtitle>Oncol Lett</addtitle><description>gene encodes the hepatoma upregulated protein (HURP), a microtubule associated protein regulating mitotic spindle dynamics, which promotes chromosomal congression and alignment during mitosis, with a potential role in tumorigenesis. In the present study,
mRNA expression was investigated by reverse transcription-quantitative PCR in oropharyngeal squamous cell carcinoma (OPSCC). Primary OPSCC tumors from 107 patients and 48 adjacent normal tissues, as well as 12 respiratory tract cancer cell lines (9 head and neck squamous cell carcinoma, 2 lung cancer and 1 normal bronchial) were utilised in the present study. mRNA expression levels of
were higher in malignant OPSCC tissues compared with in normal mucosa (P<1×10
) and significantly associated with sex and smoking status (P<0.0001). Vinorelbine
toxicity at half-maximal inhibitory concentration (IC
) was measured in the 11 cancer cell lines using an MTT assay. Sensitivity to vinorelbine was significantly correlated with HURP expression (r=0.636; P=0.035). The data indicated that
overexpression is frequent in OPSCC tissues and associated with smoking. The correlation between
mRNA expression and vinorelbine
response suggests that HURP is a potential modulator of vinorelbine response; therefore, it should be explored for its possible predictive value for the efficiency of vinorelbine treatment in this type of cancer.</description><subject>Alcohol</subject><subject>Biobanks</subject><subject>Cancer</subject><subject>Cancer research</subject><subject>Carcinoma</subject><subject>Cell division</subject><subject>Chromosomes</subject><subject>Gender</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Head & neck cancer</subject><subject>Hepatocellular carcinoma</subject><subject>Human papillomavirus</subject><subject>Liver cancer</subject><subject>Lung cancer</subject><subject>Lymphatic system</subject><subject>Messenger RNA</subject><subject>Oncology</subject><subject>Papillomavirus</subject><subject>Papillomavirus infections</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Respiratory tract cancer</subject><subject>RNA</subject><subject>Scientific equipment industry</subject><subject>Smoking</subject><subject>Software</subject><subject>Squamous cell carcinoma</subject><subject>Studies</subject><subject>Throat cancer</subject><subject>Tobacco</subject><subject>Toxicity</subject><subject>Tumorigenesis</subject><subject>Tumors</subject><subject>Values</subject><subject>Vinorelbine</subject><issn>1792-1074</issn><issn>1792-1082</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNptkk1P3DAQhqOqqCDKsdfKUqWql2z9kTjxpdIK0VIJFYTgbDnOmDV17K2dbMu_xwG6ZSvsg0fjZ17LM29RvCN4wVpBPwe3oJjiBSGMiVfFAWkELQlu6ett3FT7xVFKtzivmpO25W-KfUaJqJqaHBTufAMR_qwjpGSDR8Gg0-vLCzRc_lgi69EKVI-U75EH_RNpFbX1YVAPKZVS0FaNc91vO65mfmPHGFBWWwefAI0hZ3yI4Drr4W2xZ5RLcPR0HhbXX0-ujk_Ls_Nv34-XZ6WuuBhL3oFiPTNUK8UrIyjr6r7HDShCuWhwh2lfa1M3uGXasKbVUFGDtegqgk1fscPiy6PueuoG6DX4MSon19EOKt7JoKzcvfF2JW_CRjaYCUJwFvj0JBDDrwnSKAebNDinPIQpScpawimlFcvoh__Q2zBFn7-XKV5nQczJP-pGOZDWm5Df1bOoXHIiGKlw3WRq8QKVdw-D1cGDsTm_U_DxWUGelRtXKbhpHknaBctHUMeQUgSzbQbBcraSDE7OVpIPVsr8--cd3NJ_jcPuARViwsk</recordid><startdate>20200301</startdate><enddate>20200301</enddate><creator>Al-Khafaji, Ahmed S K</creator><creator>Pantazi, Paschalia</creator><creator>Acha-Sagredo, Amelia</creator><creator>Schache, Andrew</creator><creator>Risk, Janet M</creator><creator>Shaw, Richard J</creator><creator>Liloglou, Triantafillos</creator><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><general>D.A. 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In the present study,
mRNA expression was investigated by reverse transcription-quantitative PCR in oropharyngeal squamous cell carcinoma (OPSCC). Primary OPSCC tumors from 107 patients and 48 adjacent normal tissues, as well as 12 respiratory tract cancer cell lines (9 head and neck squamous cell carcinoma, 2 lung cancer and 1 normal bronchial) were utilised in the present study. mRNA expression levels of
were higher in malignant OPSCC tissues compared with in normal mucosa (P<1×10
) and significantly associated with sex and smoking status (P<0.0001). Vinorelbine
toxicity at half-maximal inhibitory concentration (IC
) was measured in the 11 cancer cell lines using an MTT assay. Sensitivity to vinorelbine was significantly correlated with HURP expression (r=0.636; P=0.035). The data indicated that
overexpression is frequent in OPSCC tissues and associated with smoking. The correlation between
mRNA expression and vinorelbine
response suggests that HURP is a potential modulator of vinorelbine response; therefore, it should be explored for its possible predictive value for the efficiency of vinorelbine treatment in this type of cancer.</abstract><cop>Greece</cop><pub>Spandidos Publications</pub><pmid>32194751</pmid><doi>10.3892/ol.2020.11339</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1792-1074 |
| ispartof | Oncology letters, 2020-03, Vol.19 (3), p.2502-2507 |
| issn | 1792-1074 1792-1082 |
| language | eng |
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| source | Spandidos Publications Journals; PubMed Central; EZB Electronic Journals Library |
| subjects | Alcohol Biobanks Cancer Cancer research Carcinoma Cell division Chromosomes Gender Gene expression Genes Head & neck cancer Hepatocellular carcinoma Human papillomavirus Liver cancer Lung cancer Lymphatic system Messenger RNA Oncology Papillomavirus Papillomavirus infections Polymerase chain reaction Proteins Respiratory tract cancer RNA Scientific equipment industry Smoking Software Squamous cell carcinoma Studies Throat cancer Tobacco Toxicity Tumorigenesis Tumors Values Vinorelbine |
| title | Overexpression of HURP mRNA in head and neck carcinoma and association with in vitro response to vinorelbine |
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