Hydrodynamic shear-based purification of cancer cells with enhanced tumorigenic potential
Tumor-initiating cells (TICs), a subpopulation of cancerous cells with high tumorigenic potential and stem-cell-like properties, drive tumor progression and are resistant to conventional therapies. Identification and isolation of TICs are limited by their low frequency and lack of robust markers. He...
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| Veröffentlicht in: | Integrative biology (Cambridge) 2020-02, Vol.12 (1), p.1-11 |
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| creator | Cermeño, Efraín A O'Melia, Meghan J Han, Woojin M Veith, Austin Barber, Graham Huang, Emina H Thomas, Susan N García, Andrés J |
| description | Tumor-initiating cells (TICs), a subpopulation of cancerous cells with high tumorigenic potential and stem-cell-like properties, drive tumor progression and are resistant to conventional therapies. Identification and isolation of TICs are limited by their low frequency and lack of robust markers. Here, we characterize the heterogeneous adhesive properties of a panel of human and murine cancer cells and demonstrate differences in adhesion strength among cells, which exhibit TIC properties and those that do not. These differences in adhesion strength were exploited to rapidly (~10 min) and efficiently isolate cancerous cells with increased tumorigenic potential in a label-free manner by use of a microfluidic technology. Isolated murine and human cancer cells gave rise to larger tumors with increased growth rate and higher frequency in both immunocompetent and immunocompromised mice, respectively. This rapid and label-free TIC isolation technology has the potential to be a valuable tool for facilitating research into TIC biology and the development of more efficient diagnostics and cancer therapies. |
| doi_str_mv | 10.1093/intbio/zyz038 |
| format | Article |
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Identification and isolation of TICs are limited by their low frequency and lack of robust markers. Here, we characterize the heterogeneous adhesive properties of a panel of human and murine cancer cells and demonstrate differences in adhesion strength among cells, which exhibit TIC properties and those that do not. These differences in adhesion strength were exploited to rapidly (~10 min) and efficiently isolate cancerous cells with increased tumorigenic potential in a label-free manner by use of a microfluidic technology. Isolated murine and human cancer cells gave rise to larger tumors with increased growth rate and higher frequency in both immunocompetent and immunocompromised mice, respectively. This rapid and label-free TIC isolation technology has the potential to be a valuable tool for facilitating research into TIC biology and the development of more efficient diagnostics and cancer therapies.</description><identifier>ISSN: 1757-9708</identifier><identifier>ISSN: 1757-9694</identifier><identifier>EISSN: 1757-9708</identifier><identifier>DOI: 10.1093/intbio/zyz038</identifier><identifier>PMID: 31965190</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Animals ; Breast Neoplasms - pathology ; Carcinogenesis - pathology ; Cell Adhesion ; Cell Line, Tumor ; Cell Separation - methods ; Disease Progression ; Female ; Green Fluorescent Proteins - metabolism ; Humans ; Hydrodynamics ; MCF-7 Cells ; Mice ; Mice, Inbred C57BL ; Mice, SCID ; Microfluidics ; Neoplasms - physiopathology ; Neoplastic Stem Cells - pathology ; Original ; Signal Transduction ; Stress, Mechanical</subject><ispartof>Integrative biology (Cambridge), 2020-02, Vol.12 (1), p.1-11</ispartof><rights>The Author(s) 2020. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permission@oup.com.</rights><rights>The Author(s) 2020. Published by Oxford University Press. All rights reserved. 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Identification and isolation of TICs are limited by their low frequency and lack of robust markers. Here, we characterize the heterogeneous adhesive properties of a panel of human and murine cancer cells and demonstrate differences in adhesion strength among cells, which exhibit TIC properties and those that do not. These differences in adhesion strength were exploited to rapidly (~10 min) and efficiently isolate cancerous cells with increased tumorigenic potential in a label-free manner by use of a microfluidic technology. Isolated murine and human cancer cells gave rise to larger tumors with increased growth rate and higher frequency in both immunocompetent and immunocompromised mice, respectively. This rapid and label-free TIC isolation technology has the potential to be a valuable tool for facilitating research into TIC biology and the development of more efficient diagnostics and cancer therapies.</description><subject>Animals</subject><subject>Breast Neoplasms - pathology</subject><subject>Carcinogenesis - pathology</subject><subject>Cell Adhesion</subject><subject>Cell Line, Tumor</subject><subject>Cell Separation - methods</subject><subject>Disease Progression</subject><subject>Female</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Humans</subject><subject>Hydrodynamics</subject><subject>MCF-7 Cells</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, SCID</subject><subject>Microfluidics</subject><subject>Neoplasms - physiopathology</subject><subject>Neoplastic Stem Cells - pathology</subject><subject>Original</subject><subject>Signal Transduction</subject><subject>Stress, Mechanical</subject><issn>1757-9708</issn><issn>1757-9694</issn><issn>1757-9708</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkE1LwzAYx4Mobk6PXiVfoC5Z0qa9CDKcEwZe9OAp5K1rpE1K0indp7ejOubpeXhefn_4AXCL0T1GBZlb10nr5_t-j0h-BqaYpSwpGMrPT_oJuIrxE6GMIkQvwYTgIktxgabgY93r4HXvRGMVjJURIZEiGg3bXbClVaKz3kFfQiWcMgEqU9cRftuugsZVh5mG3a7xwW6NGxCt74zrrKivwUUp6mhufusMvK-e3pbrZPP6_LJ83CSKUNIlKsuVEFIWGV3InBWLEmeSGsOYxjmVSqVykWIjGENa5ilWqcllVqSypCWmSpMZeBi57U42RqshPYiat8E2IvTcC8v_b5yt-NZ_cYZIRhkdAMkIUMHHGEx5_MWIHxzz0TEfHQ_3d6eBx-s_qeQH6Xt-rA</recordid><startdate>20200222</startdate><enddate>20200222</enddate><creator>Cermeño, Efraín A</creator><creator>O'Melia, Meghan J</creator><creator>Han, Woojin M</creator><creator>Veith, Austin</creator><creator>Barber, Graham</creator><creator>Huang, Emina H</creator><creator>Thomas, Susan N</creator><creator>García, Andrés J</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20200222</creationdate><title>Hydrodynamic shear-based purification of cancer cells with enhanced tumorigenic potential</title><author>Cermeño, Efraín A ; O'Melia, Meghan J ; Han, Woojin M ; Veith, Austin ; Barber, Graham ; Huang, Emina H ; Thomas, Susan N ; García, Andrés J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343t-c68caabb9642b8792f16b4ee77d184bcc5b251ea770db851c5e8b695bf4f14cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Breast Neoplasms - pathology</topic><topic>Carcinogenesis - pathology</topic><topic>Cell Adhesion</topic><topic>Cell Line, Tumor</topic><topic>Cell Separation - methods</topic><topic>Disease Progression</topic><topic>Female</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Humans</topic><topic>Hydrodynamics</topic><topic>MCF-7 Cells</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, SCID</topic><topic>Microfluidics</topic><topic>Neoplasms - physiopathology</topic><topic>Neoplastic Stem Cells - pathology</topic><topic>Original</topic><topic>Signal Transduction</topic><topic>Stress, Mechanical</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cermeño, Efraín A</creatorcontrib><creatorcontrib>O'Melia, Meghan J</creatorcontrib><creatorcontrib>Han, Woojin M</creatorcontrib><creatorcontrib>Veith, Austin</creatorcontrib><creatorcontrib>Barber, Graham</creatorcontrib><creatorcontrib>Huang, Emina H</creatorcontrib><creatorcontrib>Thomas, Susan N</creatorcontrib><creatorcontrib>García, Andrés J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Integrative biology (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cermeño, Efraín A</au><au>O'Melia, Meghan J</au><au>Han, Woojin M</au><au>Veith, Austin</au><au>Barber, Graham</au><au>Huang, Emina H</au><au>Thomas, Susan N</au><au>García, Andrés J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hydrodynamic shear-based purification of cancer cells with enhanced tumorigenic potential</atitle><jtitle>Integrative biology (Cambridge)</jtitle><addtitle>Integr Biol (Camb)</addtitle><date>2020-02-22</date><risdate>2020</risdate><volume>12</volume><issue>1</issue><spage>1</spage><epage>11</epage><pages>1-11</pages><issn>1757-9708</issn><issn>1757-9694</issn><eissn>1757-9708</eissn><abstract>Tumor-initiating cells (TICs), a subpopulation of cancerous cells with high tumorigenic potential and stem-cell-like properties, drive tumor progression and are resistant to conventional therapies. Identification and isolation of TICs are limited by their low frequency and lack of robust markers. Here, we characterize the heterogeneous adhesive properties of a panel of human and murine cancer cells and demonstrate differences in adhesion strength among cells, which exhibit TIC properties and those that do not. These differences in adhesion strength were exploited to rapidly (~10 min) and efficiently isolate cancerous cells with increased tumorigenic potential in a label-free manner by use of a microfluidic technology. Isolated murine and human cancer cells gave rise to larger tumors with increased growth rate and higher frequency in both immunocompetent and immunocompromised mice, respectively. This rapid and label-free TIC isolation technology has the potential to be a valuable tool for facilitating research into TIC biology and the development of more efficient diagnostics and cancer therapies.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>31965190</pmid><doi>10.1093/intbio/zyz038</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE |
| subjects | Animals Breast Neoplasms - pathology Carcinogenesis - pathology Cell Adhesion Cell Line, Tumor Cell Separation - methods Disease Progression Female Green Fluorescent Proteins - metabolism Humans Hydrodynamics MCF-7 Cells Mice Mice, Inbred C57BL Mice, SCID Microfluidics Neoplasms - physiopathology Neoplastic Stem Cells - pathology Original Signal Transduction Stress, Mechanical |
| title | Hydrodynamic shear-based purification of cancer cells with enhanced tumorigenic potential |
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