Leptin Signaling in Liver Tissue of a Transgenic Breast Cancer Mouse Model
Leptin, an adipocytokine, is secreted from various tissues including the liver. The roles of both leptin and leptin receptor (ObR) in numerous pathophysiological conditions including mammary tumor (MT) development have been reported. However, the roles of leptin signaling-related proteins in the liv...
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| Veröffentlicht in: | Curēus (Palo Alto, CA) CA), 2020-01, Vol.12 (1), p.e6737-e6737 |
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| creator | Guvenc Tuna, Bilge Cleary, Margot P Demirel, Pinar B Dogan, Soner |
| description | Leptin, an adipocytokine, is secreted from various tissues including the liver. The roles of both leptin and leptin receptor (ObR) in numerous pathophysiological conditions including mammary tumor (MT) development have been reported. However, the roles of leptin signaling-related proteins in the liver have not been reported previously in MT development. The objective of this study was to examine the expression levels of leptin and ObR in liver tissue of a transgenic breast cancer mouse model to investigate whether the roles of leptin in MT development are systemic or local. MMTV-TGF-α transgenic female mice were fed ad-libitum from week 10 up to week 74. Protein expression levels of leptin and ObR were measured in liver tissues of 74-week-old MMTV-TGF-α mice with and without MT by western blot. Serum leptin and insulin levels were measured using a enzyme-linked immunosorbent assay. Protein expression levels of leptin and ObR were similar in mice with MT compared to the ones without MT. Serum leptin and insulin levels were also not significantly different between the two groups. These results indicate that the effects of leptin signaling in MT development might be important at a local tissue level, such as mammary fat pad, and not as important at a systemic level. |
| doi_str_mv | 10.7759/cureus.6737 |
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The roles of both leptin and leptin receptor (ObR) in numerous pathophysiological conditions including mammary tumor (MT) development have been reported. However, the roles of leptin signaling-related proteins in the liver have not been reported previously in MT development. The objective of this study was to examine the expression levels of leptin and ObR in liver tissue of a transgenic breast cancer mouse model to investigate whether the roles of leptin in MT development are systemic or local. MMTV-TGF-α transgenic female mice were fed ad-libitum from week 10 up to week 74. Protein expression levels of leptin and ObR were measured in liver tissues of 74-week-old MMTV-TGF-α mice with and without MT by western blot. Serum leptin and insulin levels were measured using a enzyme-linked immunosorbent assay. Protein expression levels of leptin and ObR were similar in mice with MT compared to the ones without MT. Serum leptin and insulin levels were also not significantly different between the two groups. These results indicate that the effects of leptin signaling in MT development might be important at a local tissue level, such as mammary fat pad, and not as important at a systemic level.</description><identifier>ISSN: 2168-8184</identifier><identifier>EISSN: 2168-8184</identifier><identifier>DOI: 10.7759/cureus.6737</identifier><identifier>PMID: 32133259</identifier><language>eng</language><publisher>United States: Cureus Inc</publisher><subject>Breast cancer ; Growth factors ; Insulin ; Laboratories ; Liver ; Membranes ; Molecular weight ; Obesity ; Other ; Phosphatase ; Protein expression ; Proteins ; Tumors</subject><ispartof>Curēus (Palo Alto, CA), 2020-01, Vol.12 (1), p.e6737-e6737</ispartof><rights>Copyright © 2020, Guvenc Tuna et al.</rights><rights>Copyright © 2020, Guvenc Tuna et al. This work is published under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright © 2020, Guvenc Tuna et al. 2020 Guvenc Tuna et al.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7034766/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7034766/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32133259$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guvenc Tuna, Bilge</creatorcontrib><creatorcontrib>Cleary, Margot P</creatorcontrib><creatorcontrib>Demirel, Pinar B</creatorcontrib><creatorcontrib>Dogan, Soner</creatorcontrib><title>Leptin Signaling in Liver Tissue of a Transgenic Breast Cancer Mouse Model</title><title>Curēus (Palo Alto, CA)</title><addtitle>Cureus</addtitle><description>Leptin, an adipocytokine, is secreted from various tissues including the liver. The roles of both leptin and leptin receptor (ObR) in numerous pathophysiological conditions including mammary tumor (MT) development have been reported. However, the roles of leptin signaling-related proteins in the liver have not been reported previously in MT development. The objective of this study was to examine the expression levels of leptin and ObR in liver tissue of a transgenic breast cancer mouse model to investigate whether the roles of leptin in MT development are systemic or local. MMTV-TGF-α transgenic female mice were fed ad-libitum from week 10 up to week 74. Protein expression levels of leptin and ObR were measured in liver tissues of 74-week-old MMTV-TGF-α mice with and without MT by western blot. Serum leptin and insulin levels were measured using a enzyme-linked immunosorbent assay. Protein expression levels of leptin and ObR were similar in mice with MT compared to the ones without MT. Serum leptin and insulin levels were also not significantly different between the two groups. These results indicate that the effects of leptin signaling in MT development might be important at a local tissue level, such as mammary fat pad, and not as important at a systemic level.</description><subject>Breast cancer</subject><subject>Growth factors</subject><subject>Insulin</subject><subject>Laboratories</subject><subject>Liver</subject><subject>Membranes</subject><subject>Molecular weight</subject><subject>Obesity</subject><subject>Other</subject><subject>Phosphatase</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Tumors</subject><issn>2168-8184</issn><issn>2168-8184</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdkUtLAzEUhYMottSu3MuAG0GqeU2S2QhafFJxYV2HzMydmjLN1GSm4L83pbVUN7m5N18OJzkInRJ8JWWaXRedhy5cCcnkAepTItRIEcUP9_Y9NAxhjjEmWFIs8THqMUoYo2nWRy8TWLbWJe925kxt3SyJzcSuwCdTG0IHSVMlJpl648IMnC2SOw8mtMnYuCJCr00XIK4l1CfoqDJ1gOG2DtDHw_10_DSavD0-j28no4Jmsh1xUlJRpbzMCS3KOAKiYpdCTvOSyqziFARRVFVplgvF0pLEG4yzKjOEc2ADdLPRXXb5AsoCXOtNrZfeLoz_1o2x-u-Js5961qy0xIxLIaLAxVbAN18dhFYvbCigro2D-BxNmYyWUpLiiJ7_Q-dN5-NPrSnFhSIC80hdbqjCNyF4qHZmCNbrmPQmJr2OKdJn-_537G8o7AfyYY2s</recordid><startdate>20200122</startdate><enddate>20200122</enddate><creator>Guvenc Tuna, Bilge</creator><creator>Cleary, Margot P</creator><creator>Demirel, Pinar B</creator><creator>Dogan, Soner</creator><general>Cureus Inc</general><general>Cureus</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200122</creationdate><title>Leptin Signaling in Liver Tissue of a Transgenic Breast Cancer Mouse Model</title><author>Guvenc Tuna, Bilge ; Cleary, Margot P ; Demirel, Pinar B ; Dogan, Soner</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c297t-41d26f54db12cd297e1854d5eb2bd279f42e61828f59b6835d11d2343f9a144e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Breast cancer</topic><topic>Growth factors</topic><topic>Insulin</topic><topic>Laboratories</topic><topic>Liver</topic><topic>Membranes</topic><topic>Molecular weight</topic><topic>Obesity</topic><topic>Other</topic><topic>Phosphatase</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guvenc Tuna, Bilge</creatorcontrib><creatorcontrib>Cleary, Margot P</creatorcontrib><creatorcontrib>Demirel, Pinar B</creatorcontrib><creatorcontrib>Dogan, Soner</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Curēus (Palo Alto, CA)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guvenc Tuna, Bilge</au><au>Cleary, Margot P</au><au>Demirel, Pinar B</au><au>Dogan, Soner</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Leptin Signaling in Liver Tissue of a Transgenic Breast Cancer Mouse Model</atitle><jtitle>Curēus (Palo Alto, CA)</jtitle><addtitle>Cureus</addtitle><date>2020-01-22</date><risdate>2020</risdate><volume>12</volume><issue>1</issue><spage>e6737</spage><epage>e6737</epage><pages>e6737-e6737</pages><issn>2168-8184</issn><eissn>2168-8184</eissn><abstract>Leptin, an adipocytokine, is secreted from various tissues including the liver. The roles of both leptin and leptin receptor (ObR) in numerous pathophysiological conditions including mammary tumor (MT) development have been reported. However, the roles of leptin signaling-related proteins in the liver have not been reported previously in MT development. The objective of this study was to examine the expression levels of leptin and ObR in liver tissue of a transgenic breast cancer mouse model to investigate whether the roles of leptin in MT development are systemic or local. MMTV-TGF-α transgenic female mice were fed ad-libitum from week 10 up to week 74. Protein expression levels of leptin and ObR were measured in liver tissues of 74-week-old MMTV-TGF-α mice with and without MT by western blot. Serum leptin and insulin levels were measured using a enzyme-linked immunosorbent assay. Protein expression levels of leptin and ObR were similar in mice with MT compared to the ones without MT. 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| subjects | Breast cancer Growth factors Insulin Laboratories Liver Membranes Molecular weight Obesity Other Phosphatase Protein expression Proteins Tumors |
| title | Leptin Signaling in Liver Tissue of a Transgenic Breast Cancer Mouse Model |
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