The effects of electroporation buffer composition on cell viability and electro-transfection efficiency

Electroporation is an electro-physical, non-viral approach to perform DNA, RNA, and protein transfections of cells. Upon application of an electric field, the cell membrane is compromised, allowing the delivery of exogenous materials into cells. Cell viability and electro-transfection efficiency (eT...

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Veröffentlicht in:	Scientific reports 2020-02, Vol.10 (1), p.3053, Article 3053
Hauptverfasser:	Sherba, Joseph J., Hogquist, Stephen, Lin, Hao, Shan, Jerry W., Shreiber, David I., Zahn, Jeffrey D.
Format:	Artikel
Sprache:	eng
Schlagworte:	42/109 42/35 631/1647/2300/1851 631/61/2300/1851 82/80 96/106 96/63 Adenosine Triphosphatases - metabolism Animals Buffers Cell membranes Cell Survival - drug effects Cell viability Conductivity Deoxyribonucleic acid DNA Efficiency Electricity Electroporation Energy Energy charge Experiments FDA approval Fibroblasts Green fluorescent protein Homeostasis Humanities and Social Sciences Lidocaine Magnesium Magnesium - pharmacology Mice multidisciplinary Multiple myeloma NIH 3T3 Cells Proteins Ribonucleic acid RNA Salts Science Science (multidisciplinary) Transfection
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