Lymphatic‐to‐blood vessel transition in adult microvascular networks: A discovery made possible by a top‐down approach to biomimetic model development
Objective Emerging areas of vascular biology focus on lymphatic/blood vessel mispatterning and the regulation of endothelial cell identity. However, a fundamental question remains unanswered: Can lymphatic vessels become blood vessels in adult tissues? Leveraging a novel tissue culture model, the ob...
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| Veröffentlicht in: | Microcirculation (New York, N.Y. 1994) N.Y. 1994), 2020-02, Vol.27 (2), p.e12595-n/a |
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| container_title | Microcirculation (New York, N.Y. 1994) |
| container_volume | 27 |
| creator | Azimi, Mohammad S. Motherwell, Jessica M. Hodges, Nicholas A. Rittenhouse, Garret R. Majbour, Dima Porvasnik, Stacey L. Schmidt, Christine E. Murfee, Walter L. |
| description | Objective
Emerging areas of vascular biology focus on lymphatic/blood vessel mispatterning and the regulation of endothelial cell identity. However, a fundamental question remains unanswered: Can lymphatic vessels become blood vessels in adult tissues? Leveraging a novel tissue culture model, the objective of this study was to track lymphatic endothelial cell fate over the time course of adult microvascular network remodeling.
Methods
Cultured adult Wistar rat mesenteric tissues were labeled with BSI‐lectin and time‐lapse images were captured over five days of serum‐stimulated remodeling. Additionally, rat mesenteric tissues on day 0 and day 3 and 5 post‐culture were labeled for PECAM + LYVE‐1 or PECAM + podoplanin.
Results
Cultured networks were characterized by increases in blood capillary sprouting, lymphatic sprouting, and the number of lymphatic/blood vessel connections. Comparison of images from the same network regions identified incorporation of lymphatic vessels into blood vessels. Mosaic lymphatic/blood vessels contained lymphatic marker positive and negative endothelial cells.
Conclusions
Our results reveal the ability for lymphatic vessels to transition into blood vessels in adult microvascular networks and discover a new paradigm for investigating lymphatic/blood endothelial cell dynamics during microvascular remodeling. |
| doi_str_mv | 10.1111/micc.12595 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7033030</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2301434127</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5145-b127921b454d04c4541250b621eeccc0ce194e94144c88bc69e30335b560a9f33</originalsourceid><addsrcrecordid>eNp9kd-O1CAUxonRuOvojQ9gSLwxJl2hhWnxYpPNxD-bjPFGrwnQMw4rLRXaTnrnI_gAPp1P4lln3agXQgIEfvnOx_kIeczZGcfxovPOnfFSKnmHnHIpVNHUXN3FM6urQq2b5oQ8yPmKMdY0pbpPTiouG1GXzSn5vl26YW9G7358_TZGXGyIsaUz5AyBjsn02Y8-9tT31LRTGCmWS3E22U3BJNrDeIjpc35JL2jrs4szpIV2pgU6xJy9DUDtQg0d44DqbTygzjCkaNwe76j1sfMdoAHaxRZLtjBDiEMH_fiQ3NuZkOHRzb4iH1-_-rB5W2zfv7ncXGwLJ7mQheVlrUpuhRQtEw43bAaz65IDOOeYA64EKMGFcE1j3VpBxapKWrlmRu2qakXOj7rDZDtoHZZOJugh-c6kRUfj9d8vvd_rT3HWNcownCvy7EYgxS8T5FF32AoIwfQQp6zLinFRoa0a0af_oFdxSj1-DylZ81KJhiP1_Ehhq3NOsLs1w5m-Dl1fh65_hY7wkz_t36K_U0aAH4GDD7D8R0q_u9xsjqI_Adnrvds</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2357129481</pqid></control><display><type>article</type><title>Lymphatic‐to‐blood vessel transition in adult microvascular networks: A discovery made possible by a top‐down approach to biomimetic model development</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Azimi, Mohammad S. ; Motherwell, Jessica M. ; Hodges, Nicholas A. ; Rittenhouse, Garret R. ; Majbour, Dima ; Porvasnik, Stacey L. ; Schmidt, Christine E. ; Murfee, Walter L.</creator><creatorcontrib>Azimi, Mohammad S. ; Motherwell, Jessica M. ; Hodges, Nicholas A. ; Rittenhouse, Garret R. ; Majbour, Dima ; Porvasnik, Stacey L. ; Schmidt, Christine E. ; Murfee, Walter L.</creatorcontrib><description>Objective
Emerging areas of vascular biology focus on lymphatic/blood vessel mispatterning and the regulation of endothelial cell identity. However, a fundamental question remains unanswered: Can lymphatic vessels become blood vessels in adult tissues? Leveraging a novel tissue culture model, the objective of this study was to track lymphatic endothelial cell fate over the time course of adult microvascular network remodeling.
Methods
Cultured adult Wistar rat mesenteric tissues were labeled with BSI‐lectin and time‐lapse images were captured over five days of serum‐stimulated remodeling. Additionally, rat mesenteric tissues on day 0 and day 3 and 5 post‐culture were labeled for PECAM + LYVE‐1 or PECAM + podoplanin.
Results
Cultured networks were characterized by increases in blood capillary sprouting, lymphatic sprouting, and the number of lymphatic/blood vessel connections. Comparison of images from the same network regions identified incorporation of lymphatic vessels into blood vessels. Mosaic lymphatic/blood vessels contained lymphatic marker positive and negative endothelial cells.
Conclusions
Our results reveal the ability for lymphatic vessels to transition into blood vessels in adult microvascular networks and discover a new paradigm for investigating lymphatic/blood endothelial cell dynamics during microvascular remodeling.</description><identifier>ISSN: 1073-9688</identifier><identifier>EISSN: 1549-8719</identifier><identifier>DOI: 10.1111/micc.12595</identifier><identifier>PMID: 31584728</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Angiogenesis ; Animals ; Biomimetics ; Blood vessels ; Capillaries - diagnostic imaging ; Capillaries - metabolism ; endothelial cell ; Endothelial Cells - cytology ; Endothelial Cells - metabolism ; lymphangiogenesis ; lymphatic ; Lymphatic system ; Lymphatic Vessels - diagnostic imaging ; Lymphatic Vessels - metabolism ; Male ; microcirculation ; Models, Cardiovascular ; Rats ; Rats, Wistar ; Vascular Remodeling</subject><ispartof>Microcirculation (New York, N.Y. 1994), 2020-02, Vol.27 (2), p.e12595-n/a</ispartof><rights>2019 John Wiley & Sons Ltd</rights><rights>2019 John Wiley & Sons Ltd.</rights><rights>Copyright © 2020 John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5145-b127921b454d04c4541250b621eeccc0ce194e94144c88bc69e30335b560a9f33</citedby><cites>FETCH-LOGICAL-c5145-b127921b454d04c4541250b621eeccc0ce194e94144c88bc69e30335b560a9f33</cites><orcidid>0000-0002-8247-4722 ; 0000-0002-5865-6016</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fmicc.12595$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fmicc.12595$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31584728$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Azimi, Mohammad S.</creatorcontrib><creatorcontrib>Motherwell, Jessica M.</creatorcontrib><creatorcontrib>Hodges, Nicholas A.</creatorcontrib><creatorcontrib>Rittenhouse, Garret R.</creatorcontrib><creatorcontrib>Majbour, Dima</creatorcontrib><creatorcontrib>Porvasnik, Stacey L.</creatorcontrib><creatorcontrib>Schmidt, Christine E.</creatorcontrib><creatorcontrib>Murfee, Walter L.</creatorcontrib><title>Lymphatic‐to‐blood vessel transition in adult microvascular networks: A discovery made possible by a top‐down approach to biomimetic model development</title><title>Microcirculation (New York, N.Y. 1994)</title><addtitle>Microcirculation</addtitle><description>Objective
Emerging areas of vascular biology focus on lymphatic/blood vessel mispatterning and the regulation of endothelial cell identity. However, a fundamental question remains unanswered: Can lymphatic vessels become blood vessels in adult tissues? Leveraging a novel tissue culture model, the objective of this study was to track lymphatic endothelial cell fate over the time course of adult microvascular network remodeling.
Methods
Cultured adult Wistar rat mesenteric tissues were labeled with BSI‐lectin and time‐lapse images were captured over five days of serum‐stimulated remodeling. Additionally, rat mesenteric tissues on day 0 and day 3 and 5 post‐culture were labeled for PECAM + LYVE‐1 or PECAM + podoplanin.
Results
Cultured networks were characterized by increases in blood capillary sprouting, lymphatic sprouting, and the number of lymphatic/blood vessel connections. Comparison of images from the same network regions identified incorporation of lymphatic vessels into blood vessels. Mosaic lymphatic/blood vessels contained lymphatic marker positive and negative endothelial cells.
Conclusions
Our results reveal the ability for lymphatic vessels to transition into blood vessels in adult microvascular networks and discover a new paradigm for investigating lymphatic/blood endothelial cell dynamics during microvascular remodeling.</description><subject>Angiogenesis</subject><subject>Animals</subject><subject>Biomimetics</subject><subject>Blood vessels</subject><subject>Capillaries - diagnostic imaging</subject><subject>Capillaries - metabolism</subject><subject>endothelial cell</subject><subject>Endothelial Cells - cytology</subject><subject>Endothelial Cells - metabolism</subject><subject>lymphangiogenesis</subject><subject>lymphatic</subject><subject>Lymphatic system</subject><subject>Lymphatic Vessels - diagnostic imaging</subject><subject>Lymphatic Vessels - metabolism</subject><subject>Male</subject><subject>microcirculation</subject><subject>Models, Cardiovascular</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Vascular Remodeling</subject><issn>1073-9688</issn><issn>1549-8719</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kd-O1CAUxonRuOvojQ9gSLwxJl2hhWnxYpPNxD-bjPFGrwnQMw4rLRXaTnrnI_gAPp1P4lln3agXQgIEfvnOx_kIeczZGcfxovPOnfFSKnmHnHIpVNHUXN3FM6urQq2b5oQ8yPmKMdY0pbpPTiouG1GXzSn5vl26YW9G7358_TZGXGyIsaUz5AyBjsn02Y8-9tT31LRTGCmWS3E22U3BJNrDeIjpc35JL2jrs4szpIV2pgU6xJy9DUDtQg0d44DqbTygzjCkaNwe76j1sfMdoAHaxRZLtjBDiEMH_fiQ3NuZkOHRzb4iH1-_-rB5W2zfv7ncXGwLJ7mQheVlrUpuhRQtEw43bAaz65IDOOeYA64EKMGFcE1j3VpBxapKWrlmRu2qakXOj7rDZDtoHZZOJugh-c6kRUfj9d8vvd_rT3HWNcownCvy7EYgxS8T5FF32AoIwfQQp6zLinFRoa0a0af_oFdxSj1-DylZ81KJhiP1_Ehhq3NOsLs1w5m-Dl1fh65_hY7wkz_t36K_U0aAH4GDD7D8R0q_u9xsjqI_Adnrvds</recordid><startdate>202002</startdate><enddate>202002</enddate><creator>Azimi, Mohammad S.</creator><creator>Motherwell, Jessica M.</creator><creator>Hodges, Nicholas A.</creator><creator>Rittenhouse, Garret R.</creator><creator>Majbour, Dima</creator><creator>Porvasnik, Stacey L.</creator><creator>Schmidt, Christine E.</creator><creator>Murfee, Walter L.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-8247-4722</orcidid><orcidid>https://orcid.org/0000-0002-5865-6016</orcidid></search><sort><creationdate>202002</creationdate><title>Lymphatic‐to‐blood vessel transition in adult microvascular networks: A discovery made possible by a top‐down approach to biomimetic model development</title><author>Azimi, Mohammad S. ; Motherwell, Jessica M. ; Hodges, Nicholas A. ; Rittenhouse, Garret R. ; Majbour, Dima ; Porvasnik, Stacey L. ; Schmidt, Christine E. ; Murfee, Walter L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5145-b127921b454d04c4541250b621eeccc0ce194e94144c88bc69e30335b560a9f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Angiogenesis</topic><topic>Animals</topic><topic>Biomimetics</topic><topic>Blood vessels</topic><topic>Capillaries - diagnostic imaging</topic><topic>Capillaries - metabolism</topic><topic>endothelial cell</topic><topic>Endothelial Cells - cytology</topic><topic>Endothelial Cells - metabolism</topic><topic>lymphangiogenesis</topic><topic>lymphatic</topic><topic>Lymphatic system</topic><topic>Lymphatic Vessels - diagnostic imaging</topic><topic>Lymphatic Vessels - metabolism</topic><topic>Male</topic><topic>microcirculation</topic><topic>Models, Cardiovascular</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Vascular Remodeling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Azimi, Mohammad S.</creatorcontrib><creatorcontrib>Motherwell, Jessica M.</creatorcontrib><creatorcontrib>Hodges, Nicholas A.</creatorcontrib><creatorcontrib>Rittenhouse, Garret R.</creatorcontrib><creatorcontrib>Majbour, Dima</creatorcontrib><creatorcontrib>Porvasnik, Stacey L.</creatorcontrib><creatorcontrib>Schmidt, Christine E.</creatorcontrib><creatorcontrib>Murfee, Walter L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Microcirculation (New York, N.Y. 1994)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Azimi, Mohammad S.</au><au>Motherwell, Jessica M.</au><au>Hodges, Nicholas A.</au><au>Rittenhouse, Garret R.</au><au>Majbour, Dima</au><au>Porvasnik, Stacey L.</au><au>Schmidt, Christine E.</au><au>Murfee, Walter L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lymphatic‐to‐blood vessel transition in adult microvascular networks: A discovery made possible by a top‐down approach to biomimetic model development</atitle><jtitle>Microcirculation (New York, N.Y. 1994)</jtitle><addtitle>Microcirculation</addtitle><date>2020-02</date><risdate>2020</risdate><volume>27</volume><issue>2</issue><spage>e12595</spage><epage>n/a</epage><pages>e12595-n/a</pages><issn>1073-9688</issn><eissn>1549-8719</eissn><abstract>Objective
Emerging areas of vascular biology focus on lymphatic/blood vessel mispatterning and the regulation of endothelial cell identity. However, a fundamental question remains unanswered: Can lymphatic vessels become blood vessels in adult tissues? Leveraging a novel tissue culture model, the objective of this study was to track lymphatic endothelial cell fate over the time course of adult microvascular network remodeling.
Methods
Cultured adult Wistar rat mesenteric tissues were labeled with BSI‐lectin and time‐lapse images were captured over five days of serum‐stimulated remodeling. Additionally, rat mesenteric tissues on day 0 and day 3 and 5 post‐culture were labeled for PECAM + LYVE‐1 or PECAM + podoplanin.
Results
Cultured networks were characterized by increases in blood capillary sprouting, lymphatic sprouting, and the number of lymphatic/blood vessel connections. Comparison of images from the same network regions identified incorporation of lymphatic vessels into blood vessels. Mosaic lymphatic/blood vessels contained lymphatic marker positive and negative endothelial cells.
Conclusions
Our results reveal the ability for lymphatic vessels to transition into blood vessels in adult microvascular networks and discover a new paradigm for investigating lymphatic/blood endothelial cell dynamics during microvascular remodeling.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31584728</pmid><doi>10.1111/micc.12595</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-8247-4722</orcidid><orcidid>https://orcid.org/0000-0002-5865-6016</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1073-9688 |
| ispartof | Microcirculation (New York, N.Y. 1994), 2020-02, Vol.27 (2), p.e12595-n/a |
| issn | 1073-9688 1549-8719 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7033030 |
| source | MEDLINE; Access via Wiley Online Library |
| subjects | Angiogenesis Animals Biomimetics Blood vessels Capillaries - diagnostic imaging Capillaries - metabolism endothelial cell Endothelial Cells - cytology Endothelial Cells - metabolism lymphangiogenesis lymphatic Lymphatic system Lymphatic Vessels - diagnostic imaging Lymphatic Vessels - metabolism Male microcirculation Models, Cardiovascular Rats Rats, Wistar Vascular Remodeling |
| title | Lymphatic‐to‐blood vessel transition in adult microvascular networks: A discovery made possible by a top‐down approach to biomimetic model development |
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