Some Gammaproteobacteria are enriched within CD14+ macrophages from intestinal lamina propria of Crohn’s disease patients versus mucus
Crohn’s disease causes chronic inflammation in the gastrointestinal tract and its pathogenesis remains unclear. In the intestine of Crohn’s disease patients, CD14 + CD11 + CD163 low macrophages contribute to inflammation through the induction of Th17 cells and production of inflammatory cytokines; t...
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| Veröffentlicht in: | Scientific reports 2020-02, Vol.10 (1), p.2988-2988, Article 2988 |
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| creator | Sekido, Yuki Nishimura, Junichi Nakano, Kazuhiro Osu, Takeaki Chow, Cheryl-Emiliane T. Matsuno, Hiroshi Ogino, Takayuki Fujino, Shiki Miyoshi, Norikatsu Takahashi, Hidekazu Uemura, Mamoru Matsuda, Chu Kayama, Hisako Mori, Masaki Doki, Yuichiro Takeda, Kiyoshi Uchino, Motoi Ikeuchi, Hiroki Mizushima, Tsunekazu |
| description | Crohn’s disease causes chronic inflammation in the gastrointestinal tract and its pathogenesis remains unclear. In the intestine of Crohn’s disease patients, CD14
+
CD11
+
CD163
low
macrophages contribute to inflammation through the induction of Th17 cells and production of inflammatory cytokines; the CD14
+
CD11c
+
163
high
fraction is anti-inflammatory through the production of IL-10 in normal cases. In this report, the 16S rRNA gene amplicon sequencing method was used to identify bacteria that are specifically present in intestinal CD14
+
CD11c
+
macrophages of Crohn’s disease patients. Bacteria present in intestinal CD14
+
CD11c
+
macrophages and mucus of Crohn’s disease patients were separated into different clusters in principal coordinates analysis. There was a statistically significant increase in the relative composition of CD14
+
CD11c
+
macrophages from mucus in two phyla (
Proteobacteria
[p = 0.01] and
Actinobacteria
[p = 0.02]) and two families (
Moraxellaceae
[p |
| doi_str_mv | 10.1038/s41598-020-59937-w |
| format | Article |
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+
CD11
+
CD163
low
macrophages contribute to inflammation through the induction of Th17 cells and production of inflammatory cytokines; the CD14
+
CD11c
+
163
high
fraction is anti-inflammatory through the production of IL-10 in normal cases. In this report, the 16S rRNA gene amplicon sequencing method was used to identify bacteria that are specifically present in intestinal CD14
+
CD11c
+
macrophages of Crohn’s disease patients. Bacteria present in intestinal CD14
+
CD11c
+
macrophages and mucus of Crohn’s disease patients were separated into different clusters in principal coordinates analysis. There was a statistically significant increase in the relative composition of CD14
+
CD11c
+
macrophages from mucus in two phyla (
Proteobacteria
[p = 0.01] and
Actinobacteria
[p = 0.02]) and two families (
Moraxellaceae
[p < 0.001] and
Pseudomonadaceae
[p = 0.01]). In addition, OTU-1:
Acinetobacter
and OTU-8:
Pseudomonadaceae
tended to concentrate in the CD14
+
CD11c
+
CD163
low
subset, whereas OTU-10:
Proteus
, OTU-15:
Collinsella
tended to concentrate more in the CD14
+
CD11c
+
CD163
high
subset than the other subset and mucus.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-020-59937-w</identifier><identifier>PMID: 32076066</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/31 ; 38/22 ; 45/23 ; 45/77 ; 631/208/325/1506 ; 631/250/249/2510/1402 ; 631/250/262 ; 631/326/2565/2134 ; 692/4020/1503/257/1402 ; Adult ; Aged ; Bacteria ; CD11c antigen ; CD14 antigen ; Crohn Disease - immunology ; Crohn Disease - microbiology ; Crohn Disease - pathology ; Crohn Disease - surgery ; Crohn's disease ; Cytokines ; DNA, Bacterial - isolation & purification ; Female ; Flow cytometry ; Gammaproteobacteria - genetics ; Gammaproteobacteria - immunology ; Gammaproteobacteria - isolation & purification ; Gastrointestinal tract ; Genetic testing ; Helper cells ; Hospitals ; Humanities and Social Sciences ; Humans ; Ileum - cytology ; Ileum - immunology ; Ileum - microbiology ; Ileum - pathology ; Immunology ; Inflammation ; Inflammatory bowel disease ; Interleukin 10 ; Intestinal Mucosa - cytology ; Intestinal Mucosa - immunology ; Intestinal Mucosa - microbiology ; Intestinal Mucosa - pathology ; Intestine ; Lamina propria ; Lipopolysaccharide Receptors - metabolism ; Lymphocytes T ; Macrophages ; Macrophages - immunology ; Macrophages - metabolism ; Macrophages - microbiology ; Male ; Medicine ; Microbiota ; Middle Aged ; Mucus ; multidisciplinary ; Pseudomonadaceae ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Science ; Science (multidisciplinary) ; Statistical analysis ; Surgery ; Young Adult</subject><ispartof>Scientific reports, 2020-02, Vol.10 (1), p.2988-2988, Article 2988</ispartof><rights>The Author(s) 2020</rights><rights>This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-3b5837cd16f3bd4515ca3255ed3cd595b1549b4bd838e4e12ad2a1d5a1fd69783</citedby><cites>FETCH-LOGICAL-c540t-3b5837cd16f3bd4515ca3255ed3cd595b1549b4bd838e4e12ad2a1d5a1fd69783</cites><orcidid>0000-0002-0825-6823</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031516/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031516/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32076066$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sekido, Yuki</creatorcontrib><creatorcontrib>Nishimura, Junichi</creatorcontrib><creatorcontrib>Nakano, Kazuhiro</creatorcontrib><creatorcontrib>Osu, Takeaki</creatorcontrib><creatorcontrib>Chow, Cheryl-Emiliane T.</creatorcontrib><creatorcontrib>Matsuno, Hiroshi</creatorcontrib><creatorcontrib>Ogino, Takayuki</creatorcontrib><creatorcontrib>Fujino, Shiki</creatorcontrib><creatorcontrib>Miyoshi, Norikatsu</creatorcontrib><creatorcontrib>Takahashi, Hidekazu</creatorcontrib><creatorcontrib>Uemura, Mamoru</creatorcontrib><creatorcontrib>Matsuda, Chu</creatorcontrib><creatorcontrib>Kayama, Hisako</creatorcontrib><creatorcontrib>Mori, Masaki</creatorcontrib><creatorcontrib>Doki, Yuichiro</creatorcontrib><creatorcontrib>Takeda, Kiyoshi</creatorcontrib><creatorcontrib>Uchino, Motoi</creatorcontrib><creatorcontrib>Ikeuchi, Hiroki</creatorcontrib><creatorcontrib>Mizushima, Tsunekazu</creatorcontrib><title>Some Gammaproteobacteria are enriched within CD14+ macrophages from intestinal lamina propria of Crohn’s disease patients versus mucus</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Crohn’s disease causes chronic inflammation in the gastrointestinal tract and its pathogenesis remains unclear. In the intestine of Crohn’s disease patients, CD14
+
CD11
+
CD163
low
macrophages contribute to inflammation through the induction of Th17 cells and production of inflammatory cytokines; the CD14
+
CD11c
+
163
high
fraction is anti-inflammatory through the production of IL-10 in normal cases. In this report, the 16S rRNA gene amplicon sequencing method was used to identify bacteria that are specifically present in intestinal CD14
+
CD11c
+
macrophages of Crohn’s disease patients. Bacteria present in intestinal CD14
+
CD11c
+
macrophages and mucus of Crohn’s disease patients were separated into different clusters in principal coordinates analysis. There was a statistically significant increase in the relative composition of CD14
+
CD11c
+
macrophages from mucus in two phyla (
Proteobacteria
[p = 0.01] and
Actinobacteria
[p = 0.02]) and two families (
Moraxellaceae
[p < 0.001] and
Pseudomonadaceae
[p = 0.01]). In addition, OTU-1:
Acinetobacter
and OTU-8:
Pseudomonadaceae
tended to concentrate in the CD14
+
CD11c
+
CD163
low
subset, whereas OTU-10:
Proteus
, OTU-15:
Collinsella
tended to concentrate more in the CD14
+
CD11c
+
CD163
high
subset than the other subset and mucus.</description><subject>13/31</subject><subject>38/22</subject><subject>45/23</subject><subject>45/77</subject><subject>631/208/325/1506</subject><subject>631/250/249/2510/1402</subject><subject>631/250/262</subject><subject>631/326/2565/2134</subject><subject>692/4020/1503/257/1402</subject><subject>Adult</subject><subject>Aged</subject><subject>Bacteria</subject><subject>CD11c antigen</subject><subject>CD14 antigen</subject><subject>Crohn Disease - immunology</subject><subject>Crohn Disease - microbiology</subject><subject>Crohn Disease - pathology</subject><subject>Crohn Disease - surgery</subject><subject>Crohn's disease</subject><subject>Cytokines</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>Female</subject><subject>Flow cytometry</subject><subject>Gammaproteobacteria - genetics</subject><subject>Gammaproteobacteria - immunology</subject><subject>Gammaproteobacteria - isolation & purification</subject><subject>Gastrointestinal tract</subject><subject>Genetic testing</subject><subject>Helper cells</subject><subject>Hospitals</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Ileum - cytology</subject><subject>Ileum - immunology</subject><subject>Ileum - microbiology</subject><subject>Ileum - pathology</subject><subject>Immunology</subject><subject>Inflammation</subject><subject>Inflammatory bowel disease</subject><subject>Interleukin 10</subject><subject>Intestinal Mucosa - cytology</subject><subject>Intestinal Mucosa - immunology</subject><subject>Intestinal Mucosa - microbiology</subject><subject>Intestinal Mucosa - pathology</subject><subject>Intestine</subject><subject>Lamina propria</subject><subject>Lipopolysaccharide Receptors - metabolism</subject><subject>Lymphocytes T</subject><subject>Macrophages</subject><subject>Macrophages - immunology</subject><subject>Macrophages - metabolism</subject><subject>Macrophages - microbiology</subject><subject>Male</subject><subject>Medicine</subject><subject>Microbiota</subject><subject>Middle Aged</subject><subject>Mucus</subject><subject>multidisciplinary</subject><subject>Pseudomonadaceae</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Statistical analysis</subject><subject>Surgery</subject><subject>Young Adult</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kctu1TAQhiMEolXpC7BAltggoYCvuWyQ0KEUpEosgLU1sScnruI42EmPumPJK_T1eBLcnlIKC7wZS_PNP5e_KJ4y-opR0bxOkqm2KSmnpWpbUZe7B8Uhp1KVXHD-8N7_oDhO6Zzmp3grWfu4OBCc1hWtqsPix-fgkZyC9zDHsGDowCwYHRCISHCKzgxoyc4tg5vI5h2TL4kHE8M8wBYT6WPwxE0LpsVNMJIRfI4ka83XIqEnmxiG6ef3q0SsSwgJyQyLw2lJ5AJjWhPxq1nTk-JRD2PC49t4VHx9f_Jl86E8-3T6cfP2rDRK0qUUnWpEbSyretFZqZgyILhSaIWxqlUdU7LtZGcb0aBExsFyYFYB623V1o04Kt7sdee182hNHiTCqPO0HuKlDuD035nJDXobLnRNBVOsygIvbgVi-LbmvbV3yeA4woRhTZoL1Uoq83kz-vwf9DysMZ_phmoUr3jLMsX3VL5qShH7u2EY1dde673XOnutb7zWu1z07P4adyW_nc2A2AMpp6Ytxj-9_yP7CwfruRk</recordid><startdate>20200219</startdate><enddate>20200219</enddate><creator>Sekido, Yuki</creator><creator>Nishimura, Junichi</creator><creator>Nakano, Kazuhiro</creator><creator>Osu, Takeaki</creator><creator>Chow, Cheryl-Emiliane T.</creator><creator>Matsuno, Hiroshi</creator><creator>Ogino, Takayuki</creator><creator>Fujino, Shiki</creator><creator>Miyoshi, Norikatsu</creator><creator>Takahashi, Hidekazu</creator><creator>Uemura, Mamoru</creator><creator>Matsuda, Chu</creator><creator>Kayama, Hisako</creator><creator>Mori, Masaki</creator><creator>Doki, Yuichiro</creator><creator>Takeda, Kiyoshi</creator><creator>Uchino, Motoi</creator><creator>Ikeuchi, Hiroki</creator><creator>Mizushima, Tsunekazu</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0825-6823</orcidid></search><sort><creationdate>20200219</creationdate><title>Some Gammaproteobacteria are enriched within CD14+ macrophages from intestinal lamina propria of Crohn’s disease patients versus mucus</title><author>Sekido, Yuki ; Nishimura, Junichi ; Nakano, Kazuhiro ; Osu, Takeaki ; Chow, Cheryl-Emiliane T. ; Matsuno, Hiroshi ; Ogino, Takayuki ; Fujino, Shiki ; Miyoshi, Norikatsu ; Takahashi, Hidekazu ; Uemura, Mamoru ; Matsuda, Chu ; Kayama, Hisako ; Mori, Masaki ; Doki, Yuichiro ; Takeda, Kiyoshi ; Uchino, Motoi ; Ikeuchi, Hiroki ; Mizushima, Tsunekazu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-3b5837cd16f3bd4515ca3255ed3cd595b1549b4bd838e4e12ad2a1d5a1fd69783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>13/31</topic><topic>38/22</topic><topic>45/23</topic><topic>45/77</topic><topic>631/208/325/1506</topic><topic>631/250/249/2510/1402</topic><topic>631/250/262</topic><topic>631/326/2565/2134</topic><topic>692/4020/1503/257/1402</topic><topic>Adult</topic><topic>Aged</topic><topic>Bacteria</topic><topic>CD11c antigen</topic><topic>CD14 antigen</topic><topic>Crohn Disease - immunology</topic><topic>Crohn Disease - microbiology</topic><topic>Crohn Disease - pathology</topic><topic>Crohn Disease - surgery</topic><topic>Crohn's disease</topic><topic>Cytokines</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>Female</topic><topic>Flow cytometry</topic><topic>Gammaproteobacteria - genetics</topic><topic>Gammaproteobacteria - immunology</topic><topic>Gammaproteobacteria - isolation & purification</topic><topic>Gastrointestinal tract</topic><topic>Genetic testing</topic><topic>Helper cells</topic><topic>Hospitals</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Ileum - cytology</topic><topic>Ileum - immunology</topic><topic>Ileum - microbiology</topic><topic>Ileum - pathology</topic><topic>Immunology</topic><topic>Inflammation</topic><topic>Inflammatory bowel disease</topic><topic>Interleukin 10</topic><topic>Intestinal Mucosa - cytology</topic><topic>Intestinal Mucosa - immunology</topic><topic>Intestinal Mucosa - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sekido, Yuki</au><au>Nishimura, Junichi</au><au>Nakano, Kazuhiro</au><au>Osu, Takeaki</au><au>Chow, Cheryl-Emiliane T.</au><au>Matsuno, Hiroshi</au><au>Ogino, Takayuki</au><au>Fujino, Shiki</au><au>Miyoshi, Norikatsu</au><au>Takahashi, Hidekazu</au><au>Uemura, Mamoru</au><au>Matsuda, Chu</au><au>Kayama, Hisako</au><au>Mori, Masaki</au><au>Doki, Yuichiro</au><au>Takeda, Kiyoshi</au><au>Uchino, Motoi</au><au>Ikeuchi, Hiroki</au><au>Mizushima, Tsunekazu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Some Gammaproteobacteria are enriched within CD14+ macrophages from intestinal lamina propria of Crohn’s disease patients versus mucus</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2020-02-19</date><risdate>2020</risdate><volume>10</volume><issue>1</issue><spage>2988</spage><epage>2988</epage><pages>2988-2988</pages><artnum>2988</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Crohn’s disease causes chronic inflammation in the gastrointestinal tract and its pathogenesis remains unclear. In the intestine of Crohn’s disease patients, CD14
+
CD11
+
CD163
low
macrophages contribute to inflammation through the induction of Th17 cells and production of inflammatory cytokines; the CD14
+
CD11c
+
163
high
fraction is anti-inflammatory through the production of IL-10 in normal cases. In this report, the 16S rRNA gene amplicon sequencing method was used to identify bacteria that are specifically present in intestinal CD14
+
CD11c
+
macrophages of Crohn’s disease patients. Bacteria present in intestinal CD14
+
CD11c
+
macrophages and mucus of Crohn’s disease patients were separated into different clusters in principal coordinates analysis. There was a statistically significant increase in the relative composition of CD14
+
CD11c
+
macrophages from mucus in two phyla (
Proteobacteria
[p = 0.01] and
Actinobacteria
[p = 0.02]) and two families (
Moraxellaceae
[p < 0.001] and
Pseudomonadaceae
[p = 0.01]). In addition, OTU-1:
Acinetobacter
and OTU-8:
Pseudomonadaceae
tended to concentrate in the CD14
+
CD11c
+
CD163
low
subset, whereas OTU-10:
Proteus
, OTU-15:
Collinsella
tended to concentrate more in the CD14
+
CD11c
+
CD163
high
subset than the other subset and mucus.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>32076066</pmid><doi>10.1038/s41598-020-59937-w</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-0825-6823</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2020-02, Vol.10 (1), p.2988-2988, Article 2988 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7031516 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Springer Nature OA Free Journals; Nature Free; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 13/31 38/22 45/23 45/77 631/208/325/1506 631/250/249/2510/1402 631/250/262 631/326/2565/2134 692/4020/1503/257/1402 Adult Aged Bacteria CD11c antigen CD14 antigen Crohn Disease - immunology Crohn Disease - microbiology Crohn Disease - pathology Crohn Disease - surgery Crohn's disease Cytokines DNA, Bacterial - isolation & purification Female Flow cytometry Gammaproteobacteria - genetics Gammaproteobacteria - immunology Gammaproteobacteria - isolation & purification Gastrointestinal tract Genetic testing Helper cells Hospitals Humanities and Social Sciences Humans Ileum - cytology Ileum - immunology Ileum - microbiology Ileum - pathology Immunology Inflammation Inflammatory bowel disease Interleukin 10 Intestinal Mucosa - cytology Intestinal Mucosa - immunology Intestinal Mucosa - microbiology Intestinal Mucosa - pathology Intestine Lamina propria Lipopolysaccharide Receptors - metabolism Lymphocytes T Macrophages Macrophages - immunology Macrophages - metabolism Macrophages - microbiology Male Medicine Microbiota Middle Aged Mucus multidisciplinary Pseudomonadaceae RNA, Ribosomal, 16S - genetics rRNA 16S Science Science (multidisciplinary) Statistical analysis Surgery Young Adult |
| title | Some Gammaproteobacteria are enriched within CD14+ macrophages from intestinal lamina propria of Crohn’s disease patients versus mucus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T21%3A11%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Some%20Gammaproteobacteria%20are%20enriched%20within%20CD14+%20macrophages%20from%20intestinal%20lamina%20propria%20of%20Crohn%E2%80%99s%20disease%20patients%20versus%20mucus&rft.jtitle=Scientific%20reports&rft.au=Sekido,%20Yuki&rft.date=2020-02-19&rft.volume=10&rft.issue=1&rft.spage=2988&rft.epage=2988&rft.pages=2988-2988&rft.artnum=2988&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-020-59937-w&rft_dat=%3Cproquest_pubme%3E2359404606%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2358526291&rft_id=info:pmid/32076066&rfr_iscdi=true |