Considerations in the search for mixed-linkage (1→3),(1→4)-β-d-glucan-active endotransglycosylases

Xyloglucan endotransglucosylase, catalyzed by XTH subfamily members, is thought to play crucial roles in plant cell wall physiology. Recent discovery of endotransglycosylases active on other hemicelluloses extend our understanding of the physiological scope of endotransglycosylation in general. Discovery in Poaceaen XTHs of endotransglycosylases which act on Poaceaen-prevalent hemicelluloses, such as MLG, could reconcile the apparent incongruence between the large size of Poaceaen putative XTH families and the low xyloglucan content of their cell walls. Here, I speculate on hypothetical MLG-active endotransglycosylases and highlight potential hindrances to their discovery. It is suggested that because the location of β-(1→3) bonds within MLG oligosaccharides (MLGOs) could define their ability to act as endotranglycosylase acceptor substrates: a) thorough probing of substrate specificities necessitates the use of MLGOs created using different endo-glycanases; and b) endogenous plant exo-glycosidases, which can hinder endotranglycosylase assays by degrading acceptor substrates, might prove particularly troublesome where MLGOs are concerned.