Age related changes seen in human cornea in formalin fixed sections and on biomicroscopy in living subjects: A comparison
The purpose of our experimental research was to assess the effects of aging on the main corneal structures in healthy corneas. Small, human cornea samples were collected from 20 Caucasian subjects during surgery for traumatic lesions to the eye. Ten subjects were adults (mean age 28 years) and 10 we...
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| Veröffentlicht in: | Clinical anatomy (New York, N.Y.) N.Y.), 2020-03, Vol.33 (2), p.245-256 |
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| creator | Taurone, Samanta Miglietta, Selenia Spoletini, Marialuisa Feher, Janos Artico, Marco Papa, Veronica Matassa, Roberto Familiari, Giuseppe Gobbi, Pietro Micera, Alessandra |
| description | The purpose of our experimental research was to assess the effects of aging on the main corneal structures in healthy corneas. Small, human cornea samples were collected from 20 Caucasian subjects during surgery for traumatic lesions to the eye. Ten subjects were adults (mean age 28 years) and 10 were elderly (mean age 76 years). Morphological analysis was carried out using light microscopy and electron microscopy. Another 40 patients (20 young: mean age < 30 years; 20 elderly: mean age > 70 years) were studied in vivo by confocal microscopy. The resulting images were analyzed qualitatively, quantitatively, and statistically. The basic light microscope revealed a decrease in endothelial cell density with age accompanied by an increase in endothelial cell size. Transmission electron microscopy revealed a corneal thinning and a decrease in the number of corneal stromal cells. A marked decrease in stromal nerve fibers was observed in the older subjects compared to the younger ones. Variable pressure scanning electron microscopy (VP‐SEM) was used to make surface morphological observations and to determine the chemical composition of in vivo hydrated human corneas. Our results showed the effects of aging on normal corneal morphology highlighting the structural diversity of the corneal layers and revealing an age‐related reduction in nerve fibers, thus explaining the decreased corneal sensitivity that may be observed in the elderly. Clin. Anat. 33:245–256, 2020. © 2019 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/ca.23488 |
| format | Article |
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Small, human cornea samples were collected from 20 Caucasian subjects during surgery for traumatic lesions to the eye. Ten subjects were adults (mean age 28 years) and 10 were elderly (mean age 76 years). Morphological analysis was carried out using light microscopy and electron microscopy. Another 40 patients (20 young: mean age < 30 years; 20 elderly: mean age > 70 years) were studied in vivo by confocal microscopy. The resulting images were analyzed qualitatively, quantitatively, and statistically. The basic light microscope revealed a decrease in endothelial cell density with age accompanied by an increase in endothelial cell size. Transmission electron microscopy revealed a corneal thinning and a decrease in the number of corneal stromal cells. A marked decrease in stromal nerve fibers was observed in the older subjects compared to the younger ones. Variable pressure scanning electron microscopy (VP‐SEM) was used to make surface morphological observations and to determine the chemical composition of in vivo hydrated human corneas. Our results showed the effects of aging on normal corneal morphology highlighting the structural diversity of the corneal layers and revealing an age‐related reduction in nerve fibers, thus explaining the decreased corneal sensitivity that may be observed in the elderly. Clin. Anat. 33:245–256, 2020. © 2019 Wiley Periodicals, Inc.</description><identifier>ISSN: 0897-3806</identifier><identifier>EISSN: 1098-2353</identifier><identifier>DOI: 10.1002/ca.23488</identifier><identifier>PMID: 31595552</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Age ; age‐related changes ; Aging ; Cell density ; Cell size ; Chemical composition ; Confocal microscopy ; Cornea ; Electron microscopy ; Endothelial cells ; Experimental research ; Fibers ; Geriatrics ; ICVM ; In vivo methods and tests ; light microscope ; Light microscopy ; Microscopy ; Morphology ; nerve fibers ; Older people ; Optical microscopy ; Organic chemistry ; Original Communication ; Original Communications ; Scanning electron microscopy ; Statistical methods ; Stromal cells ; Surgery ; TEM ; Transmission electron microscopy ; VP‐SEM</subject><ispartof>Clinical anatomy (New York, N.Y.), 2020-03, Vol.33 (2), p.245-256</ispartof><rights>2019 The Authors. published by Wiley Periodicals, Inc. on behalf of American Association of Clinical Anatomists.</rights><rights>2019 The Authors. Clinical Anatomy published by Wiley Periodicals, Inc. on behalf of American Association of Clinical Anatomists.</rights><rights>2019. This article is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4388-c79acd628a0cf6b28e2becf267f6f4dbd2eb37c7033382d32e4fa2393ca5efe23</citedby><cites>FETCH-LOGICAL-c4388-c79acd628a0cf6b28e2becf267f6f4dbd2eb37c7033382d32e4fa2393ca5efe23</cites><orcidid>0000-0002-0354-894X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fca.23488$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fca.23488$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31595552$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Taurone, Samanta</creatorcontrib><creatorcontrib>Miglietta, Selenia</creatorcontrib><creatorcontrib>Spoletini, Marialuisa</creatorcontrib><creatorcontrib>Feher, Janos</creatorcontrib><creatorcontrib>Artico, Marco</creatorcontrib><creatorcontrib>Papa, Veronica</creatorcontrib><creatorcontrib>Matassa, Roberto</creatorcontrib><creatorcontrib>Familiari, Giuseppe</creatorcontrib><creatorcontrib>Gobbi, Pietro</creatorcontrib><creatorcontrib>Micera, Alessandra</creatorcontrib><title>Age related changes seen in human cornea in formalin fixed sections and on biomicroscopy in living subjects: A comparison</title><title>Clinical anatomy (New York, N.Y.)</title><addtitle>Clin Anat</addtitle><description>The purpose of our experimental research was to assess the effects of aging on the main corneal structures in healthy corneas. Small, human cornea samples were collected from 20 Caucasian subjects during surgery for traumatic lesions to the eye. Ten subjects were adults (mean age 28 years) and 10 were elderly (mean age 76 years). Morphological analysis was carried out using light microscopy and electron microscopy. Another 40 patients (20 young: mean age < 30 years; 20 elderly: mean age > 70 years) were studied in vivo by confocal microscopy. The resulting images were analyzed qualitatively, quantitatively, and statistically. The basic light microscope revealed a decrease in endothelial cell density with age accompanied by an increase in endothelial cell size. Transmission electron microscopy revealed a corneal thinning and a decrease in the number of corneal stromal cells. A marked decrease in stromal nerve fibers was observed in the older subjects compared to the younger ones. Variable pressure scanning electron microscopy (VP‐SEM) was used to make surface morphological observations and to determine the chemical composition of in vivo hydrated human corneas. Our results showed the effects of aging on normal corneal morphology highlighting the structural diversity of the corneal layers and revealing an age‐related reduction in nerve fibers, thus explaining the decreased corneal sensitivity that may be observed in the elderly. Clin. Anat. 33:245–256, 2020. © 2019 Wiley Periodicals, Inc.</description><subject>Age</subject><subject>age‐related changes</subject><subject>Aging</subject><subject>Cell density</subject><subject>Cell size</subject><subject>Chemical composition</subject><subject>Confocal microscopy</subject><subject>Cornea</subject><subject>Electron microscopy</subject><subject>Endothelial cells</subject><subject>Experimental research</subject><subject>Fibers</subject><subject>Geriatrics</subject><subject>ICVM</subject><subject>In vivo methods and tests</subject><subject>light microscope</subject><subject>Light microscopy</subject><subject>Microscopy</subject><subject>Morphology</subject><subject>nerve fibers</subject><subject>Older people</subject><subject>Optical microscopy</subject><subject>Organic chemistry</subject><subject>Original Communication</subject><subject>Original Communications</subject><subject>Scanning electron microscopy</subject><subject>Statistical methods</subject><subject>Stromal cells</subject><subject>Surgery</subject><subject>TEM</subject><subject>Transmission electron microscopy</subject><subject>VP‐SEM</subject><issn>0897-3806</issn><issn>1098-2353</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><recordid>eNp1kc1q3DAURkVpaaZJIE9QBNl041SWbEvOIjAM_YNAN-1aXMtXMxpsaSKNk87bV-6kSVvoShI6HL57P0IuSnZVMsbfG7jiolLqBVmUrFUFF7V4SRZMtbIQijUn5E1KW8bKspLqNTkRZd3Wdc0X5LBcI404wB57ajbg15hoQvTUebqZRvDUhOgR5rcNcYRhvrgfGU9o9i74RMH3NHjauTA6E0MyYXeY-cHdO7-maeq2GU3XdJll4w6iS8GfkVcWhoTnj-cp-f7xw7fV5-L266cvq-VtYSqhVGFkC6ZvuAJmbNNxhbxDY3kjbWOrvus5dkIayYQQiveCY2WBi1YYqNEiF6fk5ujdTd2IvUG_jzDoXXQjxIMO4PTfP95t9Drca8m4lI3MgnePghjuJkx7PbpkcBjAY5iS5oIJzsqqajJ6-Q-6DVP0ebxM1TOkmvZZOO8qRbRPYUqm5z61Af2rz4y-_TP8E_i7wAwUR-DBDXj4r0ivlkfhT4Bqq0I</recordid><startdate>202003</startdate><enddate>202003</enddate><creator>Taurone, Samanta</creator><creator>Miglietta, Selenia</creator><creator>Spoletini, Marialuisa</creator><creator>Feher, Janos</creator><creator>Artico, Marco</creator><creator>Papa, Veronica</creator><creator>Matassa, Roberto</creator><creator>Familiari, Giuseppe</creator><creator>Gobbi, Pietro</creator><creator>Micera, Alessandra</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>24P</scope><scope>WIN</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TS</scope><scope>H94</scope><scope>JQ2</scope><scope>K9.</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0354-894X</orcidid></search><sort><creationdate>202003</creationdate><title>Age related changes seen in human cornea in formalin fixed sections and on biomicroscopy in living subjects: A comparison</title><author>Taurone, Samanta ; 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Variable pressure scanning electron microscopy (VP‐SEM) was used to make surface morphological observations and to determine the chemical composition of in vivo hydrated human corneas. Our results showed the effects of aging on normal corneal morphology highlighting the structural diversity of the corneal layers and revealing an age‐related reduction in nerve fibers, thus explaining the decreased corneal sensitivity that may be observed in the elderly. Clin. Anat. 33:245–256, 2020. © 2019 Wiley Periodicals, Inc.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>31595552</pmid><doi>10.1002/ca.23488</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-0354-894X</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Clinical anatomy (New York, N.Y.), 2020-03, Vol.33 (2), p.245-256 |
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| subjects | Age age‐related changes Aging Cell density Cell size Chemical composition Confocal microscopy Cornea Electron microscopy Endothelial cells Experimental research Fibers Geriatrics ICVM In vivo methods and tests light microscope Light microscopy Microscopy Morphology nerve fibers Older people Optical microscopy Organic chemistry Original Communication Original Communications Scanning electron microscopy Statistical methods Stromal cells Surgery TEM Transmission electron microscopy VP‐SEM |
| title | Age related changes seen in human cornea in formalin fixed sections and on biomicroscopy in living subjects: A comparison |
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