Recombinant production of a hard‐to‐express membrane‐bound cytochrome P450 in different yeasts—Comparison of physiology and productivity

Cytochrome P450s comprise one of the largest protein superfamilies. They occur in every kingdom of life and catalyse a variety of essential reactions. Their production is of utmost interest regarding biotransformation and structure‐function elucidation. However, they have proven hard to express due...

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Veröffentlicht in:	Yeast (Chichester, England) England), 2020-02, Vol.37 (2), p.217-226
Hauptverfasser:	Hausjell, Johanna, Schendl, Dominik, Weissensteiner, Julia, Molitor, Christian, Halbwirth, Heidi, Spadiut, Oliver
Format:	Artikel
Sprache:	eng
Schlagworte:	Bioreactors Biotransformation Cell Culture Techniques - methods chalcone 3‐hydroxylase Cultivation techniques Cytochrome Cytochrome P-450 Enzyme System - biosynthesis Cytochrome P-450 Enzyme System - genetics Cytochrome P450 Fungal Proteins - genetics Fungal Proteins - metabolism Gene Expression Regulation, Fungal Hydroxylase physiology Pichia - enzymology Pichia - genetics Pichia - metabolism Pichia pastoris recombinant protein production Recombinant Proteins - genetics Recombinant Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Structure-function relationships Yeast
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