Sonic Hedgehog Regulates Bone Fracture Healing
Bone fracture healing involves the combination of intramembranous and endochondral ossification. It is known that Indian hedgehog (Ihh) promotes chondrogenesis during fracture healing. Meanwhile, Sonic hedgehog (Shh), which is involved in ontogeny, has been reported to be involved in fracture healin...
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| Veröffentlicht in: | International journal of molecular sciences 2020-01, Vol.21 (2), p.677 |
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| container_title | International journal of molecular sciences |
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| creator | Takebe, Hiroaki Shalehin, Nazmus Hosoya, Akihiro Shimo, Tsuyoshi Irie, Kazuharu |
| description | Bone fracture healing involves the combination of intramembranous and endochondral ossification. It is known that Indian hedgehog (Ihh) promotes chondrogenesis during fracture healing. Meanwhile, Sonic hedgehog (Shh), which is involved in ontogeny, has been reported to be involved in fracture healing, but the details had not been clarified. In this study, we demonstrated that Shh participated in fracture healing. Six-week-old Sprague-Dawley rats and Gli-CreER
; tdTomato mice were used in this study. The right rib bones of experimental animals were fractured. The localization of Shh and Gli1 during fracture healing was examined. The localization of Gli1 progeny cells and osterix (Osx)-positive cells was similar during fracture healing. Runt-related transcription factor 2 (Runx2) and Osx, both of which are osteoblast markers, were observed on the surface of the new bone matrix and chondrocytes on day seven after fracture. Shh and Gli1 were co-localized with Runx2 and Osx. These findings suggest that Shh is involved in intramembranous and endochondral ossification during fracture healing. |
| doi_str_mv | 10.3390/ijms21020677 |
| format | Article |
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; tdTomato mice were used in this study. The right rib bones of experimental animals were fractured. The localization of Shh and Gli1 during fracture healing was examined. The localization of Gli1 progeny cells and osterix (Osx)-positive cells was similar during fracture healing. Runt-related transcription factor 2 (Runx2) and Osx, both of which are osteoblast markers, were observed on the surface of the new bone matrix and chondrocytes on day seven after fracture. Shh and Gli1 were co-localized with Runx2 and Osx. These findings suggest that Shh is involved in intramembranous and endochondral ossification during fracture healing.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms21020677</identifier><identifier>PMID: 31968603</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Biomedical materials ; Bone healing ; Bone marrow ; Bone matrix ; Cartilage ; Cbfa-1 protein ; Chondrocytes ; Chondrogenesis ; Communication ; Endochondral bone ; Fractures ; Healing ; Hedgehog protein ; Hematoma ; Homeostasis ; Localization ; Ontogeny ; Ossification ; Progeny ; Proteins</subject><ispartof>International journal of molecular sciences, 2020-01, Vol.21 (2), p.677</ispartof><rights>2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-a43272e157ce010ccd800355fc365ed10bcbf11a115bc0e28fab0ba50fdd99ad3</citedby><cites>FETCH-LOGICAL-c478t-a43272e157ce010ccd800355fc365ed10bcbf11a115bc0e28fab0ba50fdd99ad3</cites><orcidid>0000-0002-7811-2366</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013927/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013927/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31968603$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takebe, Hiroaki</creatorcontrib><creatorcontrib>Shalehin, Nazmus</creatorcontrib><creatorcontrib>Hosoya, Akihiro</creatorcontrib><creatorcontrib>Shimo, Tsuyoshi</creatorcontrib><creatorcontrib>Irie, Kazuharu</creatorcontrib><title>Sonic Hedgehog Regulates Bone Fracture Healing</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Bone fracture healing involves the combination of intramembranous and endochondral ossification. It is known that Indian hedgehog (Ihh) promotes chondrogenesis during fracture healing. Meanwhile, Sonic hedgehog (Shh), which is involved in ontogeny, has been reported to be involved in fracture healing, but the details had not been clarified. In this study, we demonstrated that Shh participated in fracture healing. Six-week-old Sprague-Dawley rats and Gli-CreER
; tdTomato mice were used in this study. The right rib bones of experimental animals were fractured. The localization of Shh and Gli1 during fracture healing was examined. The localization of Gli1 progeny cells and osterix (Osx)-positive cells was similar during fracture healing. Runt-related transcription factor 2 (Runx2) and Osx, both of which are osteoblast markers, were observed on the surface of the new bone matrix and chondrocytes on day seven after fracture. Shh and Gli1 were co-localized with Runx2 and Osx. These findings suggest that Shh is involved in intramembranous and endochondral ossification during fracture healing.</description><subject>Biomedical materials</subject><subject>Bone healing</subject><subject>Bone marrow</subject><subject>Bone matrix</subject><subject>Cartilage</subject><subject>Cbfa-1 protein</subject><subject>Chondrocytes</subject><subject>Chondrogenesis</subject><subject>Communication</subject><subject>Endochondral bone</subject><subject>Fractures</subject><subject>Healing</subject><subject>Hedgehog protein</subject><subject>Hematoma</subject><subject>Homeostasis</subject><subject>Localization</subject><subject>Ontogeny</subject><subject>Ossification</subject><subject>Progeny</subject><subject>Proteins</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkc1Lw0AQxRdRbK3ePEvAiwdTZ3ez-bgIWqwVCoIf52WzmaQpabbuJoL_vSutpXqaGebHY94bQs4pjDnP4KZerhyjwCBOkgMypBFjIfjhcK8fkBPnlgCMM5EdkwGnWZzGwIdk_GraWgczLCpcmCp4wapvVIcuuDctBlOrdNdb9IBq6rY6JUelahyebeuIvE8f3iazcP78-DS5m4c6StIuVBFnCUMqEo1AQesiBeBClJrHAgsKuc5LShWlIteALC1VDrkSUBZFlqmCj8jtRnfd5yssNLadVY1c23ql7Jc0qpZ_N229kJX5lAlQnrHEC1xtBaz56NF1clU7jU2jWjS9k4xHPpzIJ-TRy3_o0vS29fYkE1Eacxqz1FPXG0pb45zFcncMBfnzCLn_CI9f7BvYwb_J82_-noNz</recordid><startdate>20200120</startdate><enddate>20200120</enddate><creator>Takebe, Hiroaki</creator><creator>Shalehin, Nazmus</creator><creator>Hosoya, Akihiro</creator><creator>Shimo, Tsuyoshi</creator><creator>Irie, Kazuharu</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7811-2366</orcidid></search><sort><creationdate>20200120</creationdate><title>Sonic Hedgehog Regulates Bone Fracture Healing</title><author>Takebe, Hiroaki ; 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| source | MDPI - Multidisciplinary Digital Publishing Institute; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Biomedical materials Bone healing Bone marrow Bone matrix Cartilage Cbfa-1 protein Chondrocytes Chondrogenesis Communication Endochondral bone Fractures Healing Hedgehog protein Hematoma Homeostasis Localization Ontogeny Ossification Progeny Proteins |
| title | Sonic Hedgehog Regulates Bone Fracture Healing |
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