A two‐enzyme constituted mixture to improve the degradation of Arthrospira platensis microalga cell wall for monogastric diets
The main goal of this study was to test a rational combination of pre‐selected carbohydrate‐active enzymes (CAZymes) and sulphatases, individually or in combination, in order to evaluate its capacity to disrupt Arthrospira platensis cell wall, allowing the release of its valuable nutritional bioacti...
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| creator | Coelho, Diogo Lopes, Paula A. Cardoso, Vânia Ponte, Patrícia Brás, Joana Madeira, Marta S. Alfaia, Cristina M. Bandarra, Narcisa M. Fontes, Carlos M. G. A. Prates, José A. M. |
| description | The main goal of this study was to test a rational combination of pre‐selected carbohydrate‐active enzymes (CAZymes) and sulphatases, individually or in combination, in order to evaluate its capacity to disrupt Arthrospira platensis cell wall, allowing the release of its valuable nutritional bioactive compounds. By the end, a two‐enzyme constituted mixture (Mix), composed by a lysozyme and a α‐amylase, was incubated with A. platensis suspension. The microalga cell wall disruption was evaluated through the amount of reducing sugars released from the cell wall complemented with the oligosaccharide profile by HPLC. An increase of the amount of reducing sugars up to 2.42 g/L in microalgae treated with the Mix relative to no treatment (p |
| doi_str_mv | 10.1111/jpn.13239 |
| format | Article |
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G. A. ; Prates, José A. M.</creator><creatorcontrib>Coelho, Diogo ; Lopes, Paula A. ; Cardoso, Vânia ; Ponte, Patrícia ; Brás, Joana ; Madeira, Marta S. ; Alfaia, Cristina M. ; Bandarra, Narcisa M. ; Fontes, Carlos M. G. A. ; Prates, José A. M.</creatorcontrib><description>The main goal of this study was to test a rational combination of pre‐selected carbohydrate‐active enzymes (CAZymes) and sulphatases, individually or in combination, in order to evaluate its capacity to disrupt Arthrospira platensis cell wall, allowing the release of its valuable nutritional bioactive compounds. By the end, a two‐enzyme constituted mixture (Mix), composed by a lysozyme and a α‐amylase, was incubated with A. platensis suspension. The microalga cell wall disruption was evaluated through the amount of reducing sugars released from the cell wall complemented with the oligosaccharide profile by HPLC. An increase of the amount of reducing sugars up to 2.42 g/L in microalgae treated with the Mix relative to no treatment (p < .05), as well as a 7‐fold increase of oligosaccharides amount (p < .001), were obtained. With resort of fluorescence microscopy, a 36% reduction of fluorescence intensity (p < .001) was observed using Calcofluor White staining. In the supernatant, the Mix caused a 1.34‐fold increase in protein content (p = .018) relative to the control. Similarly, n‐6 polyunsaturated fatty acids (PUFA) (p = .007), in particular 18:2n‐6 (p = .016), monounsaturated fatty acids (MUFA) (p = .049) and chlorophyll a (p = .025) contents were higher in the supernatant of microalgae treated with the enzyme mixture in relation to the control. Taken together, these results point towards the disclosure of a novel two‐enzyme mixture able to partial degrade A. platensis cell wall, improving its nutrients bioavailability for monogastric diets with the cost‐effective advantage use of microalgae in animal feed industry.</description><identifier>ISSN: 0931-2439</identifier><identifier>EISSN: 1439-0396</identifier><identifier>DOI: 10.1111/jpn.13239</identifier><identifier>PMID: 31680348</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Algae ; Animal feed ; Animal Feed - analysis ; Animals ; Arthrospira platensis ; Bioactive compounds ; Bioavailability ; Carbohydrates ; carbohydrate‐active enzymes ; cell wall ; Cell Wall - chemistry ; Cell walls ; Chlorophyll ; Cloning, Molecular ; Diet ; Disruption ; Enzymes ; Enzymes - chemistry ; Enzymes - metabolism ; Fatty acids ; Feed industry ; Feeds ; Fluorescence ; Fluorescence microscopy ; Food Handling ; Gene Expression Regulation, Plant ; High-performance liquid chromatography ; Liquid chromatography ; Lysozyme ; Microalgae - chemistry ; Nutrient content ; Nutrients ; Oligosaccharides ; Original ; Polyunsaturated fatty acids ; Protein folding ; Protein Stability ; Recombinant Proteins ; reducing sugars ; Spirulina - chemistry ; Sugar ; total proteins ; α-Amylase</subject><ispartof>Journal of animal physiology and animal nutrition, 2020-01, Vol.104 (1), p.310-321</ispartof><rights>2019 The Authors. published by Blackwell Verlag GmbH.</rights><rights>2019 The Authors. Journal of Animal Physiology and Animal Nutrition published by Blackwell Verlag GmbH.</rights><rights>2020 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4439-b15accc589e3e7be16eb81bfccb085b598a8915e94319c5c13d52f613984fbef3</citedby><cites>FETCH-LOGICAL-c4439-b15accc589e3e7be16eb81bfccb085b598a8915e94319c5c13d52f613984fbef3</cites><orcidid>0000-0003-1032-5987</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjpn.13239$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjpn.13239$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31680348$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Coelho, Diogo</creatorcontrib><creatorcontrib>Lopes, Paula A.</creatorcontrib><creatorcontrib>Cardoso, Vânia</creatorcontrib><creatorcontrib>Ponte, Patrícia</creatorcontrib><creatorcontrib>Brás, Joana</creatorcontrib><creatorcontrib>Madeira, Marta S.</creatorcontrib><creatorcontrib>Alfaia, Cristina M.</creatorcontrib><creatorcontrib>Bandarra, Narcisa M.</creatorcontrib><creatorcontrib>Fontes, Carlos M. G. A.</creatorcontrib><creatorcontrib>Prates, José A. M.</creatorcontrib><title>A two‐enzyme constituted mixture to improve the degradation of Arthrospira platensis microalga cell wall for monogastric diets</title><title>Journal of animal physiology and animal nutrition</title><addtitle>J Anim Physiol Anim Nutr (Berl)</addtitle><description>The main goal of this study was to test a rational combination of pre‐selected carbohydrate‐active enzymes (CAZymes) and sulphatases, individually or in combination, in order to evaluate its capacity to disrupt Arthrospira platensis cell wall, allowing the release of its valuable nutritional bioactive compounds. By the end, a two‐enzyme constituted mixture (Mix), composed by a lysozyme and a α‐amylase, was incubated with A. platensis suspension. The microalga cell wall disruption was evaluated through the amount of reducing sugars released from the cell wall complemented with the oligosaccharide profile by HPLC. An increase of the amount of reducing sugars up to 2.42 g/L in microalgae treated with the Mix relative to no treatment (p < .05), as well as a 7‐fold increase of oligosaccharides amount (p < .001), were obtained. With resort of fluorescence microscopy, a 36% reduction of fluorescence intensity (p < .001) was observed using Calcofluor White staining. In the supernatant, the Mix caused a 1.34‐fold increase in protein content (p = .018) relative to the control. Similarly, n‐6 polyunsaturated fatty acids (PUFA) (p = .007), in particular 18:2n‐6 (p = .016), monounsaturated fatty acids (MUFA) (p = .049) and chlorophyll a (p = .025) contents were higher in the supernatant of microalgae treated with the enzyme mixture in relation to the control. Taken together, these results point towards the disclosure of a novel two‐enzyme mixture able to partial degrade A. platensis cell wall, improving its nutrients bioavailability for monogastric diets with the cost‐effective advantage use of microalgae in animal feed industry.</description><subject>Algae</subject><subject>Animal feed</subject><subject>Animal Feed - analysis</subject><subject>Animals</subject><subject>Arthrospira platensis</subject><subject>Bioactive compounds</subject><subject>Bioavailability</subject><subject>Carbohydrates</subject><subject>carbohydrate‐active enzymes</subject><subject>cell wall</subject><subject>Cell Wall - chemistry</subject><subject>Cell walls</subject><subject>Chlorophyll</subject><subject>Cloning, Molecular</subject><subject>Diet</subject><subject>Disruption</subject><subject>Enzymes</subject><subject>Enzymes - chemistry</subject><subject>Enzymes - metabolism</subject><subject>Fatty acids</subject><subject>Feed industry</subject><subject>Feeds</subject><subject>Fluorescence</subject><subject>Fluorescence microscopy</subject><subject>Food Handling</subject><subject>Gene Expression Regulation, Plant</subject><subject>High-performance liquid chromatography</subject><subject>Liquid chromatography</subject><subject>Lysozyme</subject><subject>Microalgae - chemistry</subject><subject>Nutrient content</subject><subject>Nutrients</subject><subject>Oligosaccharides</subject><subject>Original</subject><subject>Polyunsaturated fatty acids</subject><subject>Protein folding</subject><subject>Protein Stability</subject><subject>Recombinant Proteins</subject><subject>reducing sugars</subject><subject>Spirulina - chemistry</subject><subject>Sugar</subject><subject>total proteins</subject><subject>α-Amylase</subject><issn>0931-2439</issn><issn>1439-0396</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNp1kc1u1DAUhS1ERYfCghdAltjAYlrfOMnYG6RRVX6qCljA2nKcmxmPEjvYTofpqo_AM_IkeJhSARJeXFvXn4_u8SHkGbBTyOtsM7pT4AWXD8gMSi7njMv6IZkxyWFe5MYxeRzjhjFYVKx-RI451ILxUszI7ZKmrf9x-x3dzW5AaryLyaYpYUsH-y1NAWny1A5j8Nf5uEba4iroVifrHfUdXYa0Dj6ONmg69jqhizbmtyZ43a80Ndj3dKtz6Xygg3d-pWMK1tDWYopPyFGn-4hP7_YT8uXNxefzd_Orj2_fny-v5qbcO2qg0saYSkjkuGgQamwENJ0xDRNVU0mhhYQKZclBmsoAb6uiq4FLUXYNdvyEvD7ojlMzYGvQpaB7NQY76LBTXlv1942za7Xy12rBWMmYyAIv7wSC_zphTGqwcW9OO_RTVAUHkAVwxjL64h9046fgsr1MlcBFzeWeenWg8k_FGLC7HwaY2ueqcq7qV66Zff7n9Pfk7yAzcHYAtrbH3f-V1OWnDwfJn_U9sYc</recordid><startdate>202001</startdate><enddate>202001</enddate><creator>Coelho, Diogo</creator><creator>Lopes, Paula A.</creator><creator>Cardoso, Vânia</creator><creator>Ponte, Patrícia</creator><creator>Brás, Joana</creator><creator>Madeira, Marta S.</creator><creator>Alfaia, Cristina M.</creator><creator>Bandarra, Narcisa M.</creator><creator>Fontes, Carlos M. G. A.</creator><creator>Prates, José A. M.</creator><general>Wiley Subscription Services, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1032-5987</orcidid></search><sort><creationdate>202001</creationdate><title>A two‐enzyme constituted mixture to improve the degradation of Arthrospira platensis microalga cell wall for monogastric diets</title><author>Coelho, Diogo ; Lopes, Paula A. ; Cardoso, Vânia ; Ponte, Patrícia ; Brás, Joana ; Madeira, Marta S. ; Alfaia, Cristina M. ; Bandarra, Narcisa M. ; Fontes, Carlos M. G. A. ; Prates, José A. 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M.</creatorcontrib><collection>Wiley Online Library (Open Access Collection)</collection><collection>Wiley Online Library (Open Access Collection)</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of animal physiology and animal nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Coelho, Diogo</au><au>Lopes, Paula A.</au><au>Cardoso, Vânia</au><au>Ponte, Patrícia</au><au>Brás, Joana</au><au>Madeira, Marta S.</au><au>Alfaia, Cristina M.</au><au>Bandarra, Narcisa M.</au><au>Fontes, Carlos M. G. A.</au><au>Prates, José A. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A two‐enzyme constituted mixture to improve the degradation of Arthrospira platensis microalga cell wall for monogastric diets</atitle><jtitle>Journal of animal physiology and animal nutrition</jtitle><addtitle>J Anim Physiol Anim Nutr (Berl)</addtitle><date>2020-01</date><risdate>2020</risdate><volume>104</volume><issue>1</issue><spage>310</spage><epage>321</epage><pages>310-321</pages><issn>0931-2439</issn><eissn>1439-0396</eissn><abstract>The main goal of this study was to test a rational combination of pre‐selected carbohydrate‐active enzymes (CAZymes) and sulphatases, individually or in combination, in order to evaluate its capacity to disrupt Arthrospira platensis cell wall, allowing the release of its valuable nutritional bioactive compounds. By the end, a two‐enzyme constituted mixture (Mix), composed by a lysozyme and a α‐amylase, was incubated with A. platensis suspension. The microalga cell wall disruption was evaluated through the amount of reducing sugars released from the cell wall complemented with the oligosaccharide profile by HPLC. An increase of the amount of reducing sugars up to 2.42 g/L in microalgae treated with the Mix relative to no treatment (p < .05), as well as a 7‐fold increase of oligosaccharides amount (p < .001), were obtained. With resort of fluorescence microscopy, a 36% reduction of fluorescence intensity (p < .001) was observed using Calcofluor White staining. In the supernatant, the Mix caused a 1.34‐fold increase in protein content (p = .018) relative to the control. Similarly, n‐6 polyunsaturated fatty acids (PUFA) (p = .007), in particular 18:2n‐6 (p = .016), monounsaturated fatty acids (MUFA) (p = .049) and chlorophyll a (p = .025) contents were higher in the supernatant of microalgae treated with the enzyme mixture in relation to the control. Taken together, these results point towards the disclosure of a novel two‐enzyme mixture able to partial degrade A. platensis cell wall, improving its nutrients bioavailability for monogastric diets with the cost‐effective advantage use of microalgae in animal feed industry.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31680348</pmid><doi>10.1111/jpn.13239</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-1032-5987</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Algae Animal feed Animal Feed - analysis Animals Arthrospira platensis Bioactive compounds Bioavailability Carbohydrates carbohydrate‐active enzymes cell wall Cell Wall - chemistry Cell walls Chlorophyll Cloning, Molecular Diet Disruption Enzymes Enzymes - chemistry Enzymes - metabolism Fatty acids Feed industry Feeds Fluorescence Fluorescence microscopy Food Handling Gene Expression Regulation, Plant High-performance liquid chromatography Liquid chromatography Lysozyme Microalgae - chemistry Nutrient content Nutrients Oligosaccharides Original Polyunsaturated fatty acids Protein folding Protein Stability Recombinant Proteins reducing sugars Spirulina - chemistry Sugar total proteins α-Amylase |
| title | A two‐enzyme constituted mixture to improve the degradation of Arthrospira platensis microalga cell wall for monogastric diets |
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