Rapid detection of deformed wing virus in honeybee using ultra-rapid qPCR and a DNA-chip

Fast and accurate detection of viral RNA pathogens is important in apiculture. A polymerase chain reaction (PCR)-based detection method has been developed, which is simple, specific, and sensitive. In this study, we rapidly (in 1 min) synthesized cDNA from the RNA of deformed wing virus (DWV)-infect...

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Veröffentlicht in:	Journal of veterinary science (Suwŏn-si, Korea) Korea), 2020-01, Vol.21 (1), p.27-35
Hauptverfasser:	Kim, Jung Min, Lim, Su Jin, Kim, SoMin, Kim, MoonJung, Kim, ByoungHee, Tai, Truong A, Kim, Seonmi, Yoon, ByoungSu
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Beekeeping - methods Bees - virology DNA, Complementary - analysis DNA, Viral - analysis Oligonucleotide Array Sequence Analysis - veterinary Original Real-Time Polymerase Chain Reaction - methods RNA Viruses - genetics RNA Viruses - isolation & purification RNA, Viral - analysis
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