Genome Mining of Alkaloidal Terpenoids from a Hybrid Terpene and Nonribosomal Peptide Biosynthetic Pathway
Biosynthetic pathways containing multiple core enzymes have potential to produce structurally complex natural products. Here we mined a fungal gene cluster that contains two predicted terpene cyclases (TCs) and a nonribosomal peptide synthetase (NRPS). We showed the flv pathway produces flavunoidine...
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| Veröffentlicht in: | Journal of the American Chemical Society 2020-01, Vol.142 (2), p.710-714 |
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| container_title | Journal of the American Chemical Society |
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| creator | Yee, Danielle A Kakule, Thomas B Cheng, Wei Chen, Mengbin Chong, Christine T. Y Hai, Yang Hang, Leibniz F Hung, Yiu-Sun Liu, Nicholas Ohashi, Masao Okorafor, Ikechukwu C Song, Yongxiang Tang, Mancheng Zhang, Zhuan Tang, Yi |
| description | Biosynthetic pathways containing multiple core enzymes have potential to produce structurally complex natural products. Here we mined a fungal gene cluster that contains two predicted terpene cyclases (TCs) and a nonribosomal peptide synthetase (NRPS). We showed the flv pathway produces flavunoidine 1, an alkaloidal terpenoid. The core of 1 is a tetracyclic, cage-like, and oxygenated sesquiterpene that is connected to dimethylcadaverine via a C–N bond and is acylated with 5,5-dimethyl-l-pipecolate. The roles of all flv enzymes are established on the basis of metabolite analysis from heterologous expression. |
| doi_str_mv | 10.1021/jacs.9b13046 |
| format | Article |
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| subjects | Alkaloids - chemistry Genome Peptides - chemistry Ribosomes - chemistry Terpenes - chemistry |
| title | Genome Mining of Alkaloidal Terpenoids from a Hybrid Terpene and Nonribosomal Peptide Biosynthetic Pathway |
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