Biological and physiological characteristics of human cumulus cells in adherent culture condition
Cumulus cells, as oocyte nurse cells, provide a suitable microenvironment with growth factors and cellular interactions required for oocyte maturation. Thus, these cells may serve as a natural niche for studies of female germ cell development. Cumulus cells may help attain a better understanding of...
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| Veröffentlicht in: | International Journal of Reproductive BioMedicine 2020-01, Vol.18 (1), p.1-10 |
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| container_title | International Journal of Reproductive BioMedicine |
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| creator | Sadat Tahajjodi, Somayyeh Farashahi Yazd, Ehsan Agha-Rahimi, Azam Aflatoonian, Reza Ali Khalili, Mohammad Mohammadi, Mahnaz Aflatoonian, Behrouz |
| description | Cumulus cells, as oocyte nurse cells, provide a suitable microenvironment with growth factors and cellular interactions required for oocyte maturation. Thus, these cells may serve as a natural niche for
studies of female germ cell development. Cumulus cells may help attain a better understanding of the causes of infertility in women and eventually improve the outcomes of cases that respond poorly to standard infertility treatment.
The aim of this study was to isolate, culture, and investigate the biological characteristics of human cumulus cells.
In this experimental study, cumulus cells were isolated, cultured, and characterized using reverse transcription-polymerase chain reaction analyses of specific genes including
,
,
,
, and
The presence of vimentin, a structural protein, was examined via immunofluorescent staining. Moreover, levels of anti-mullerian hormone (AMH) and progesterone secretion by cumulus cells were measured with ELISA after 2, 4, 12, 24, and 48 hr of culture.
In adherent culture, human cumulus cells expressed specific genes and markers as well as secreted AMH and progesterone into the medium.
Cumulus cells secrete AMH and progesterone in an adherent culture and might be applicable for
maturation (IVM) and
gametogenesis (IVG) studies. |
| doi_str_mv | 10.18502/ijrm.v18i1.6189 |
| format | Article |
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studies of female germ cell development. Cumulus cells may help attain a better understanding of the causes of infertility in women and eventually improve the outcomes of cases that respond poorly to standard infertility treatment.
The aim of this study was to isolate, culture, and investigate the biological characteristics of human cumulus cells.
In this experimental study, cumulus cells were isolated, cultured, and characterized using reverse transcription-polymerase chain reaction analyses of specific genes including
,
,
,
, and
The presence of vimentin, a structural protein, was examined via immunofluorescent staining. Moreover, levels of anti-mullerian hormone (AMH) and progesterone secretion by cumulus cells were measured with ELISA after 2, 4, 12, 24, and 48 hr of culture.
In adherent culture, human cumulus cells expressed specific genes and markers as well as secreted AMH and progesterone into the medium.
Cumulus cells secrete AMH and progesterone in an adherent culture and might be applicable for
maturation (IVM) and
gametogenesis (IVG) studies.</description><identifier>ISSN: 2476-4108</identifier><identifier>EISSN: 2476-3772</identifier><identifier>DOI: 10.18502/ijrm.v18i1.6189</identifier><identifier>PMID: 32043066</identifier><language>eng</language><publisher>Iran: Yazd Shahid Sadoughi University of Medical Sciences, Research and Clinical Center for Infertility</publisher><subject>Cellular biology ; cumulus cells, conditioned medium, in vitro maturation, in vitro gametogenesis, niche ; In vitro fertilization ; Infertility</subject><ispartof>International Journal of Reproductive BioMedicine, 2020-01, Vol.18 (1), p.1-10</ispartof><rights>Copyright © 2020 Tahajjodi et al.</rights><rights>2020. This work is published under https://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright © 2020 Tahajjodi et al. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6996122/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6996122/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32043066$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sadat Tahajjodi, Somayyeh</creatorcontrib><creatorcontrib>Farashahi Yazd, Ehsan</creatorcontrib><creatorcontrib>Agha-Rahimi, Azam</creatorcontrib><creatorcontrib>Aflatoonian, Reza</creatorcontrib><creatorcontrib>Ali Khalili, Mohammad</creatorcontrib><creatorcontrib>Mohammadi, Mahnaz</creatorcontrib><creatorcontrib>Aflatoonian, Behrouz</creatorcontrib><title>Biological and physiological characteristics of human cumulus cells in adherent culture condition</title><title>International Journal of Reproductive BioMedicine</title><addtitle>Int J Reprod Biomed</addtitle><description>Cumulus cells, as oocyte nurse cells, provide a suitable microenvironment with growth factors and cellular interactions required for oocyte maturation. Thus, these cells may serve as a natural niche for
studies of female germ cell development. Cumulus cells may help attain a better understanding of the causes of infertility in women and eventually improve the outcomes of cases that respond poorly to standard infertility treatment.
The aim of this study was to isolate, culture, and investigate the biological characteristics of human cumulus cells.
In this experimental study, cumulus cells were isolated, cultured, and characterized using reverse transcription-polymerase chain reaction analyses of specific genes including
,
,
,
, and
The presence of vimentin, a structural protein, was examined via immunofluorescent staining. Moreover, levels of anti-mullerian hormone (AMH) and progesterone secretion by cumulus cells were measured with ELISA after 2, 4, 12, 24, and 48 hr of culture.
In adherent culture, human cumulus cells expressed specific genes and markers as well as secreted AMH and progesterone into the medium.
Cumulus cells secrete AMH and progesterone in an adherent culture and might be applicable for
maturation (IVM) and
gametogenesis (IVG) studies.</description><subject>Cellular biology</subject><subject>cumulus cells, conditioned medium, in vitro maturation, in vitro gametogenesis, niche</subject><subject>In vitro fertilization</subject><subject>Infertility</subject><issn>2476-4108</issn><issn>2476-3772</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><sourceid>DOA</sourceid><recordid>eNpdkctvFDEMxiMEolXpnROKxIXLLnEyk5lckKDiUakSFzhH3jx2ssokSzJTqf89oV0e5WTL_vTzZ5uQl8C2MPaMvw2HMm9vYQywlTCqJ-Scd4PciGHgT095B2w8I5e1HhhjIHsALp6TM8FZJ5iU5wQ_hBzzPhiMFJOlx-mu_q2YCQuaxZVQl2AqzZ5O64yJmnVe41qpcTFWGhJFO7ni0tI6cVmLoyYnG5aQ0wvyzGOs7vIUL8j3Tx-_XX3Z3Hz9fH31_mZjhWTLRniDDoV0qMB64wdw0hrmDUDfI3IjuVXoxp0bOs87gc5zxYWQXlihFIgLcv3AtRkP-ljCjOVOZwz6vpDLXmNpW0SnjUHOGXbW73jHxx36wQxSgGzTmfOmsd49sI7rbnbWtMUKxkfQx50UJr3Pt1oqJYHzBnhzApT8Y3V10XOov46FyeW1ai560Y-DHLsmff2f9JDXktqp7lVMAUjWVK_-dfTHyu9Pip_W4aVN</recordid><startdate>20200101</startdate><enddate>20200101</enddate><creator>Sadat Tahajjodi, Somayyeh</creator><creator>Farashahi Yazd, Ehsan</creator><creator>Agha-Rahimi, Azam</creator><creator>Aflatoonian, Reza</creator><creator>Ali Khalili, Mohammad</creator><creator>Mohammadi, Mahnaz</creator><creator>Aflatoonian, Behrouz</creator><general>Yazd Shahid Sadoughi University of Medical Sciences, Research and Clinical Center for Infertility</general><general>Knowledge E</general><general>Shahid Sadoughi University of Medical Sciences</general><scope>NPM</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20200101</creationdate><title>Biological and physiological characteristics of human cumulus cells in adherent culture condition</title><author>Sadat Tahajjodi, Somayyeh ; 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Thus, these cells may serve as a natural niche for
studies of female germ cell development. Cumulus cells may help attain a better understanding of the causes of infertility in women and eventually improve the outcomes of cases that respond poorly to standard infertility treatment.
The aim of this study was to isolate, culture, and investigate the biological characteristics of human cumulus cells.
In this experimental study, cumulus cells were isolated, cultured, and characterized using reverse transcription-polymerase chain reaction analyses of specific genes including
,
,
,
, and
The presence of vimentin, a structural protein, was examined via immunofluorescent staining. Moreover, levels of anti-mullerian hormone (AMH) and progesterone secretion by cumulus cells were measured with ELISA after 2, 4, 12, 24, and 48 hr of culture.
In adherent culture, human cumulus cells expressed specific genes and markers as well as secreted AMH and progesterone into the medium.
Cumulus cells secrete AMH and progesterone in an adherent culture and might be applicable for
maturation (IVM) and
gametogenesis (IVG) studies.</abstract><cop>Iran</cop><pub>Yazd Shahid Sadoughi University of Medical Sciences, Research and Clinical Center for Infertility</pub><pmid>32043066</pmid><doi>10.18502/ijrm.v18i1.6189</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 2476-4108 |
| ispartof | International Journal of Reproductive BioMedicine, 2020-01, Vol.18 (1), p.1-10 |
| issn | 2476-4108 2476-3772 |
| language | eng |
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| source | DOAJ Directory of Open Access Journals; PubMed Central |
| subjects | Cellular biology cumulus cells, conditioned medium, in vitro maturation, in vitro gametogenesis, niche In vitro fertilization Infertility |
| title | Biological and physiological characteristics of human cumulus cells in adherent culture condition |
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