Nucleolin promotes Ang II‐induced phenotypic transformation of vascular smooth muscle cells by regulating EGF and PDGF‐BB
RNA‐binding properties of nucleolin play a fundamental role in regulating cell growth and proliferation. We have previously shown that nucleolin plays an important regulatory role in the phenotypic transformation of vascular smooth muscle cells (VSMCs) induced by angiotensin II (Ang II). In the pres...
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| Veröffentlicht in: | Journal of cellular and molecular medicine 2020-01, Vol.24 (2), p.1917-1933 |
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| creator | Fang, Li Wang, Kang‐Kai Zhang, Peng‐Fei Li, Tao Xiao, Zhi‐Lin Yang, Mei Yu, Zai‐Xin |
| description | RNA‐binding properties of nucleolin play a fundamental role in regulating cell growth and proliferation. We have previously shown that nucleolin plays an important regulatory role in the phenotypic transformation of vascular smooth muscle cells (VSMCs) induced by angiotensin II (Ang II). In the present study, we aimed to investigate the molecular mechanism of nucleolin‐mediated phenotypic transformation of VSMCs induced by Ang II. Epidermal growth factor (EGF) and platelet‐derived growth factor (PDGF) inhibitors were used to observe the effect of Ang II on phenotypic transformation of VSMCs. The regulatory role of nucleolin in the phenotypic transformation of VSMCs was identified by nucleolin gene mutation, gene overexpression and RNA interference technology. Moreover, we elucidated the molecular mechanism underlying the regulatory effect of nucleolin on phenotypic transformation of VSMCs. EGF and PDGF‐BB played an important role in the phenotypic transformation of VSMCs induced by Ang II. Nucleolin exerted a positive regulatory effect on the expression and secretion of EGF and PDGF‐BB. In addition, nucleolin could bind to the 5′ untranslated region (UTR) of EGF and PDGF‐BB mRNA, and such binding up‐regulated the stability and expression of EGF and PDGF‐BB mRNA, promoting Ang II‐induced phenotypic transformation of VSMCs. |
| doi_str_mv | 10.1111/jcmm.14888 |
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We have previously shown that nucleolin plays an important regulatory role in the phenotypic transformation of vascular smooth muscle cells (VSMCs) induced by angiotensin II (Ang II). In the present study, we aimed to investigate the molecular mechanism of nucleolin‐mediated phenotypic transformation of VSMCs induced by Ang II. Epidermal growth factor (EGF) and platelet‐derived growth factor (PDGF) inhibitors were used to observe the effect of Ang II on phenotypic transformation of VSMCs. The regulatory role of nucleolin in the phenotypic transformation of VSMCs was identified by nucleolin gene mutation, gene overexpression and RNA interference technology. Moreover, we elucidated the molecular mechanism underlying the regulatory effect of nucleolin on phenotypic transformation of VSMCs. EGF and PDGF‐BB played an important role in the phenotypic transformation of VSMCs induced by Ang II. Nucleolin exerted a positive regulatory effect on the expression and secretion of EGF and PDGF‐BB. In addition, nucleolin could bind to the 5′ untranslated region (UTR) of EGF and PDGF‐BB mRNA, and such binding up‐regulated the stability and expression of EGF and PDGF‐BB mRNA, promoting Ang II‐induced phenotypic transformation of VSMCs.</description><identifier>ISSN: 1582-1838</identifier><identifier>EISSN: 1582-4934</identifier><identifier>DOI: 10.1111/jcmm.14888</identifier><identifier>PMID: 31893573</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>5' Untranslated Regions ; 5' Untranslated Regions - genetics ; Angiotensin ; Angiotensin II ; Angiotensin II - pharmacology ; Antibiotics ; Becaplermin - genetics ; Becaplermin - metabolism ; Bioinformatics ; Biotechnology ; Cell culture ; Cell division ; Cell growth ; Cell Line ; Cell Line, Transformed ; EGF ; Enzymes ; Epidermal growth factor ; Epidermal Growth Factor - genetics ; Epidermal Growth Factor - metabolism ; Gene Expression Regulation ; Genes ; Genes, Reporter ; Genetic transformation ; Genomes ; Genotype & phenotype ; Luciferases - metabolism ; Monoclonal antibodies ; mRNA ; Muscle, Smooth, Vascular - cytology ; Muscles ; Myocytes, Smooth Muscle - metabolism ; Nucleolin ; Original ; PDGF‐BB ; Phenotype ; phenotypic transformation ; Phosphoproteins - metabolism ; Plasmids ; Platelet-derived growth factor ; Point mutation ; Polyclonal antibodies ; Protein Binding ; Proteins ; Regulation ; RNA Stability ; RNA-Binding Proteins - metabolism ; RNA-mediated interference ; Smooth muscle ; Studies ; vascular smooth muscle cells</subject><ispartof>Journal of cellular and molecular medicine, 2020-01, Vol.24 (2), p.1917-1933</ispartof><rights>2019 The Authors. published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd</rights><rights>2019 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.</rights><rights>2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4768-dab884af1631e66ba5e1ad73b46c888fcebd13dd59fc3949acfd8498e36db4003</citedby><cites>FETCH-LOGICAL-c4768-dab884af1631e66ba5e1ad73b46c888fcebd13dd59fc3949acfd8498e36db4003</cites><orcidid>0000-0001-9487-2376 ; 0000-0002-1076-7729</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6991698/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6991698/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,1417,11562,27924,27925,45574,45575,46052,46476,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31893573$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fang, Li</creatorcontrib><creatorcontrib>Wang, Kang‐Kai</creatorcontrib><creatorcontrib>Zhang, Peng‐Fei</creatorcontrib><creatorcontrib>Li, Tao</creatorcontrib><creatorcontrib>Xiao, Zhi‐Lin</creatorcontrib><creatorcontrib>Yang, Mei</creatorcontrib><creatorcontrib>Yu, Zai‐Xin</creatorcontrib><title>Nucleolin promotes Ang II‐induced phenotypic transformation of vascular smooth muscle cells by regulating EGF and PDGF‐BB</title><title>Journal of cellular and molecular medicine</title><addtitle>J Cell Mol Med</addtitle><description>RNA‐binding properties of nucleolin play a fundamental role in regulating cell growth and proliferation. We have previously shown that nucleolin plays an important regulatory role in the phenotypic transformation of vascular smooth muscle cells (VSMCs) induced by angiotensin II (Ang II). In the present study, we aimed to investigate the molecular mechanism of nucleolin‐mediated phenotypic transformation of VSMCs induced by Ang II. Epidermal growth factor (EGF) and platelet‐derived growth factor (PDGF) inhibitors were used to observe the effect of Ang II on phenotypic transformation of VSMCs. The regulatory role of nucleolin in the phenotypic transformation of VSMCs was identified by nucleolin gene mutation, gene overexpression and RNA interference technology. Moreover, we elucidated the molecular mechanism underlying the regulatory effect of nucleolin on phenotypic transformation of VSMCs. EGF and PDGF‐BB played an important role in the phenotypic transformation of VSMCs induced by Ang II. Nucleolin exerted a positive regulatory effect on the expression and secretion of EGF and PDGF‐BB. In addition, nucleolin could bind to the 5′ untranslated region (UTR) of EGF and PDGF‐BB mRNA, and such binding up‐regulated the stability and expression of EGF and PDGF‐BB mRNA, promoting Ang II‐induced phenotypic transformation of VSMCs.</description><subject>5' Untranslated Regions</subject><subject>5' Untranslated Regions - genetics</subject><subject>Angiotensin</subject><subject>Angiotensin II</subject><subject>Angiotensin II - pharmacology</subject><subject>Antibiotics</subject><subject>Becaplermin - genetics</subject><subject>Becaplermin - metabolism</subject><subject>Bioinformatics</subject><subject>Biotechnology</subject><subject>Cell culture</subject><subject>Cell division</subject><subject>Cell growth</subject><subject>Cell Line</subject><subject>Cell Line, Transformed</subject><subject>EGF</subject><subject>Enzymes</subject><subject>Epidermal growth factor</subject><subject>Epidermal Growth Factor - genetics</subject><subject>Epidermal Growth Factor - metabolism</subject><subject>Gene Expression Regulation</subject><subject>Genes</subject><subject>Genes, Reporter</subject><subject>Genetic transformation</subject><subject>Genomes</subject><subject>Genotype & phenotype</subject><subject>Luciferases - metabolism</subject><subject>Monoclonal antibodies</subject><subject>mRNA</subject><subject>Muscle, Smooth, Vascular - cytology</subject><subject>Muscles</subject><subject>Myocytes, Smooth Muscle - metabolism</subject><subject>Nucleolin</subject><subject>Original</subject><subject>PDGF‐BB</subject><subject>Phenotype</subject><subject>phenotypic transformation</subject><subject>Phosphoproteins - metabolism</subject><subject>Plasmids</subject><subject>Platelet-derived growth factor</subject><subject>Point mutation</subject><subject>Polyclonal antibodies</subject><subject>Protein Binding</subject><subject>Proteins</subject><subject>Regulation</subject><subject>RNA Stability</subject><subject>RNA-Binding Proteins - metabolism</subject><subject>RNA-mediated interference</subject><subject>Smooth muscle</subject><subject>Studies</subject><subject>vascular smooth muscle cells</subject><issn>1582-1838</issn><issn>1582-4934</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU-PEyEYh4nRuH_04gcwJF7MJl1hYChcTHbrttbsqgc9EwaYlmaAEWbW9GDiR_Az-kmktm7Uw3KB5H3y8P7yA-AZRue4nFcb7f05ppzzB-AY17yaUEHow8Mbc8KPwEnOG4QIw0Q8BkcEc0HqKTkG396PurOxcwH2Kfo42Awvwgoulz-__3DBjNoa2K9tiMO2dxoOSYXcxuTV4GKAsYW3KuuxUwlmH-Owhn7MxQi17boMmy1MdlXGgyvSq8UcqmDgxzeLedFfXj4Bj1rVZfv0cJ-Cz_OrT7O3k-sPi-Xs4nqi6ZTxiVEN51S1mBFsGWtUbbEyU9JQpkvqVtvGYGJMLVpNBBVKt4ZTwS1hpqEl9il4vff2Y-Ot0TaUHJ3sk_MqbWVUTv47CW4tV_FWMiEwE7wIXh4EKX4ZbR6kd3kXUQUbxywrQirEK1Hv_nrxH7qJYwolnqzoVCDEMcX3UoRyggSitFBne0qnmHOy7d3KGMld93LXvfzdfYGf_x3yDv1TdgHwHvjqOru9RyXfzW5u9tJfJc69Uw</recordid><startdate>202001</startdate><enddate>202001</enddate><creator>Fang, Li</creator><creator>Wang, Kang‐Kai</creator><creator>Zhang, Peng‐Fei</creator><creator>Li, Tao</creator><creator>Xiao, Zhi‐Lin</creator><creator>Yang, Mei</creator><creator>Yu, Zai‐Xin</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9487-2376</orcidid><orcidid>https://orcid.org/0000-0002-1076-7729</orcidid></search><sort><creationdate>202001</creationdate><title>Nucleolin promotes Ang II‐induced phenotypic transformation of vascular smooth muscle cells by regulating EGF and PDGF‐BB</title><author>Fang, Li ; Wang, Kang‐Kai ; Zhang, Peng‐Fei ; Li, Tao ; Xiao, Zhi‐Lin ; Yang, Mei ; Yu, Zai‐Xin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4768-dab884af1631e66ba5e1ad73b46c888fcebd13dd59fc3949acfd8498e36db4003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>5' Untranslated Regions</topic><topic>5' Untranslated Regions - genetics</topic><topic>Angiotensin</topic><topic>Angiotensin II</topic><topic>Angiotensin II - pharmacology</topic><topic>Antibiotics</topic><topic>Becaplermin - genetics</topic><topic>Becaplermin - metabolism</topic><topic>Bioinformatics</topic><topic>Biotechnology</topic><topic>Cell culture</topic><topic>Cell division</topic><topic>Cell growth</topic><topic>Cell Line</topic><topic>Cell Line, Transformed</topic><topic>EGF</topic><topic>Enzymes</topic><topic>Epidermal growth factor</topic><topic>Epidermal Growth Factor - genetics</topic><topic>Epidermal Growth Factor - metabolism</topic><topic>Gene Expression Regulation</topic><topic>Genes</topic><topic>Genes, Reporter</topic><topic>Genetic transformation</topic><topic>Genomes</topic><topic>Genotype & phenotype</topic><topic>Luciferases - metabolism</topic><topic>Monoclonal antibodies</topic><topic>mRNA</topic><topic>Muscle, Smooth, Vascular - cytology</topic><topic>Muscles</topic><topic>Myocytes, Smooth Muscle - metabolism</topic><topic>Nucleolin</topic><topic>Original</topic><topic>PDGF‐BB</topic><topic>Phenotype</topic><topic>phenotypic transformation</topic><topic>Phosphoproteins - metabolism</topic><topic>Plasmids</topic><topic>Platelet-derived growth factor</topic><topic>Point mutation</topic><topic>Polyclonal antibodies</topic><topic>Protein Binding</topic><topic>Proteins</topic><topic>Regulation</topic><topic>RNA Stability</topic><topic>RNA-Binding Proteins - metabolism</topic><topic>RNA-mediated interference</topic><topic>Smooth muscle</topic><topic>Studies</topic><topic>vascular smooth muscle cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fang, Li</creatorcontrib><creatorcontrib>Wang, Kang‐Kai</creatorcontrib><creatorcontrib>Zhang, Peng‐Fei</creatorcontrib><creatorcontrib>Li, Tao</creatorcontrib><creatorcontrib>Xiao, Zhi‐Lin</creatorcontrib><creatorcontrib>Yang, Mei</creatorcontrib><creatorcontrib>Yu, Zai‐Xin</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Wiley Online Library Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of cellular and molecular medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fang, Li</au><au>Wang, Kang‐Kai</au><au>Zhang, Peng‐Fei</au><au>Li, Tao</au><au>Xiao, Zhi‐Lin</au><au>Yang, Mei</au><au>Yu, Zai‐Xin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nucleolin promotes Ang II‐induced phenotypic transformation of vascular smooth muscle cells by regulating EGF and PDGF‐BB</atitle><jtitle>Journal of cellular and molecular medicine</jtitle><addtitle>J Cell Mol Med</addtitle><date>2020-01</date><risdate>2020</risdate><volume>24</volume><issue>2</issue><spage>1917</spage><epage>1933</epage><pages>1917-1933</pages><issn>1582-1838</issn><eissn>1582-4934</eissn><abstract>RNA‐binding properties of nucleolin play a fundamental role in regulating cell growth and proliferation. We have previously shown that nucleolin plays an important regulatory role in the phenotypic transformation of vascular smooth muscle cells (VSMCs) induced by angiotensin II (Ang II). In the present study, we aimed to investigate the molecular mechanism of nucleolin‐mediated phenotypic transformation of VSMCs induced by Ang II. Epidermal growth factor (EGF) and platelet‐derived growth factor (PDGF) inhibitors were used to observe the effect of Ang II on phenotypic transformation of VSMCs. The regulatory role of nucleolin in the phenotypic transformation of VSMCs was identified by nucleolin gene mutation, gene overexpression and RNA interference technology. Moreover, we elucidated the molecular mechanism underlying the regulatory effect of nucleolin on phenotypic transformation of VSMCs. EGF and PDGF‐BB played an important role in the phenotypic transformation of VSMCs induced by Ang II. Nucleolin exerted a positive regulatory effect on the expression and secretion of EGF and PDGF‐BB. In addition, nucleolin could bind to the 5′ untranslated region (UTR) of EGF and PDGF‐BB mRNA, and such binding up‐regulated the stability and expression of EGF and PDGF‐BB mRNA, promoting Ang II‐induced phenotypic transformation of VSMCs.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>31893573</pmid><doi>10.1111/jcmm.14888</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0001-9487-2376</orcidid><orcidid>https://orcid.org/0000-0002-1076-7729</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 5' Untranslated Regions 5' Untranslated Regions - genetics Angiotensin Angiotensin II Angiotensin II - pharmacology Antibiotics Becaplermin - genetics Becaplermin - metabolism Bioinformatics Biotechnology Cell culture Cell division Cell growth Cell Line Cell Line, Transformed EGF Enzymes Epidermal growth factor Epidermal Growth Factor - genetics Epidermal Growth Factor - metabolism Gene Expression Regulation Genes Genes, Reporter Genetic transformation Genomes Genotype & phenotype Luciferases - metabolism Monoclonal antibodies mRNA Muscle, Smooth, Vascular - cytology Muscles Myocytes, Smooth Muscle - metabolism Nucleolin Original PDGF‐BB Phenotype phenotypic transformation Phosphoproteins - metabolism Plasmids Platelet-derived growth factor Point mutation Polyclonal antibodies Protein Binding Proteins Regulation RNA Stability RNA-Binding Proteins - metabolism RNA-mediated interference Smooth muscle Studies vascular smooth muscle cells |
| title | Nucleolin promotes Ang II‐induced phenotypic transformation of vascular smooth muscle cells by regulating EGF and PDGF‐BB |
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