Engineered disulfide crosslinking to measure conformational changes in the 26S proteasome

The 26S proteasome is a multisubunit ATP-dependent peptidase complex mediating most regulated protein degradation in eukaryotes. The proteasome undergoes several coordinated conformational changes during catalysis that activate it for substrate processing and functionally couple distinct enzymatic a...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Methods in enzymology 2019, Vol.619, p.145-159
Hauptverfasser:	Reed, Randi G, Tomko, Jr, Robert J
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - analogs & derivatives Adenosine Triphosphate - pharmacology Cross-Linking Reagents - chemistry Disulfides - chemistry Models, Molecular Proteasome Endopeptidase Complex - chemistry Proteasome Inhibitors - chemistry Proteasome Inhibitors - pharmacology Protein Conformation - drug effects Protein Subunits - chemistry Saccharomyces cerevisiae - chemistry Saccharomyces cerevisiae Proteins - antagonists & inhibitors Saccharomyces cerevisiae Proteins - chemistry
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!