Zika virus targets the human thymic epithelium
Previous work showed that the thymus can be infected by RNA viruses as HIV and HTLV-1. We thus hypothesized that the thymus might also be infected by the Zika virus (ZIKV). Herein we provide compelling evidence that ZIKV targets human thymic epithelial cells (TEC) in vivo and in vitro . ZIKV-infecti...
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| Veröffentlicht in: | Scientific reports 2020-01, Vol.10 (1), p.1378-1378, Article 1378 |
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| creator | Messias, Carolina V. Loss-Morais, Guilherme Carvalho, Joseane Biso de González, Mariela N. Cunha, Daniela P. Vasconcelos, Zilton Arge, Luis W. P. Farias-de-Oliveira, Désio A. Gerber, Alexandra L. Portari, Elyzabeth A. Ferreira, Nilma Raphael, Lidiane M. S. Bonaldo, Myrna C. Riederer, Ingo Lopes Moreira, Maria E. Cotta-de-Almeida, Vinicius Vasconcelos, Ana T. R. Mendes-da-Cruz, Daniella A. Savino, Wilson |
| description | Previous work showed that the thymus can be infected by RNA viruses as HIV and HTLV-1. We thus hypothesized that the thymus might also be infected by the Zika virus (ZIKV). Herein we provide compelling evidence that ZIKV targets human thymic epithelial cells (TEC)
in vivo
and
in vitro
. ZIKV-infection enhances keratinization of TEC, with a decrease in proliferation and increase in cell death. Moreover, ZIKV modulates a high amount of coding RNAs with upregulation of genes related to cell adhesion and migration, as well as non-coding genes including miRNAs, circRNAs and lncRNAs. Moreover, we observed enhanced attachment of lymphoblastic T-cells to infected TEC, as well as virus transfer to those cells. Lastly, alterations in thymuses from babies congenitally infected were seen, with the presence of viral envelope protein in TEC. Taken together, our data reveals that the thymus, particularly the thymic epithelium, is a target for the ZIKV with changes in the expression of molecules that are relevant for interactions with developing thymocytes. |
| doi_str_mv | 10.1038/s41598-020-58135-y |
| format | Article |
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in vivo
and
in vitro
. ZIKV-infection enhances keratinization of TEC, with a decrease in proliferation and increase in cell death. Moreover, ZIKV modulates a high amount of coding RNAs with upregulation of genes related to cell adhesion and migration, as well as non-coding genes including miRNAs, circRNAs and lncRNAs. Moreover, we observed enhanced attachment of lymphoblastic T-cells to infected TEC, as well as virus transfer to those cells. Lastly, alterations in thymuses from babies congenitally infected were seen, with the presence of viral envelope protein in TEC. Taken together, our data reveals that the thymus, particularly the thymic epithelium, is a target for the ZIKV with changes in the expression of molecules that are relevant for interactions with developing thymocytes.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-020-58135-y</identifier><identifier>PMID: 31992777</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38/1 ; 38/39 ; 38/77 ; 631/250/1620 ; 631/250/255/2514 ; 82/51 ; 96/2 ; 96/31 ; 96/63 ; Animals ; Cell adhesion ; Cell adhesion & migration ; Cell death ; Cell migration ; Chlorocebus aethiops ; Epithelial cells ; Epithelial Cells - metabolism ; Epithelial Cells - pathology ; Epithelial Cells - virology ; Epithelium ; Epithelium - metabolism ; Epithelium - pathology ; Epithelium - virology ; HIV ; Human immunodeficiency virus ; Humanities and Social Sciences ; Humans ; Keratinization ; Lymphocytes T ; multidisciplinary ; Multidisciplinary Sciences ; RNA viruses ; Science ; Science & Technology ; Science & Technology - Other Topics ; Science (multidisciplinary) ; Thymocytes ; Thymocytes - metabolism ; Thymocytes - pathology ; Thymocytes - virology ; Thymus ; Thymus Gland - metabolism ; Thymus Gland - pathology ; Thymus Gland - virology ; Vector-borne diseases ; Vero Cells ; Viral envelope proteins ; Viral Tropism ; Zika virus ; Zika Virus - physiology ; Zika Virus Infection - metabolism ; Zika Virus Infection - pathology</subject><ispartof>Scientific reports, 2020-01, Vol.10 (1), p.1378-1378, Article 1378</ispartof><rights>The Author(s) 2020</rights><rights>This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). 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P.</creatorcontrib><creatorcontrib>Farias-de-Oliveira, Désio A.</creatorcontrib><creatorcontrib>Gerber, Alexandra L.</creatorcontrib><creatorcontrib>Portari, Elyzabeth A.</creatorcontrib><creatorcontrib>Ferreira, Nilma</creatorcontrib><creatorcontrib>Raphael, Lidiane M. S.</creatorcontrib><creatorcontrib>Bonaldo, Myrna C.</creatorcontrib><creatorcontrib>Riederer, Ingo</creatorcontrib><creatorcontrib>Lopes Moreira, Maria E.</creatorcontrib><creatorcontrib>Cotta-de-Almeida, Vinicius</creatorcontrib><creatorcontrib>Vasconcelos, Ana T. R.</creatorcontrib><creatorcontrib>Mendes-da-Cruz, Daniella A.</creatorcontrib><creatorcontrib>Savino, Wilson</creatorcontrib><title>Zika virus targets the human thymic epithelium</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>SCI REP-UK</addtitle><addtitle>Sci Rep</addtitle><description>Previous work showed that the thymus can be infected by RNA viruses as HIV and HTLV-1. We thus hypothesized that the thymus might also be infected by the Zika virus (ZIKV). Herein we provide compelling evidence that ZIKV targets human thymic epithelial cells (TEC)
in vivo
and
in vitro
. ZIKV-infection enhances keratinization of TEC, with a decrease in proliferation and increase in cell death. Moreover, ZIKV modulates a high amount of coding RNAs with upregulation of genes related to cell adhesion and migration, as well as non-coding genes including miRNAs, circRNAs and lncRNAs. Moreover, we observed enhanced attachment of lymphoblastic T-cells to infected TEC, as well as virus transfer to those cells. Lastly, alterations in thymuses from babies congenitally infected were seen, with the presence of viral envelope protein in TEC. 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P.</au><au>Farias-de-Oliveira, Désio A.</au><au>Gerber, Alexandra L.</au><au>Portari, Elyzabeth A.</au><au>Ferreira, Nilma</au><au>Raphael, Lidiane M. S.</au><au>Bonaldo, Myrna C.</au><au>Riederer, Ingo</au><au>Lopes Moreira, Maria E.</au><au>Cotta-de-Almeida, Vinicius</au><au>Vasconcelos, Ana T. R.</au><au>Mendes-da-Cruz, Daniella A.</au><au>Savino, Wilson</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Zika virus targets the human thymic epithelium</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><stitle>SCI REP-UK</stitle><addtitle>Sci Rep</addtitle><date>2020-01-28</date><risdate>2020</risdate><volume>10</volume><issue>1</issue><spage>1378</spage><epage>1378</epage><pages>1378-1378</pages><artnum>1378</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Previous work showed that the thymus can be infected by RNA viruses as HIV and HTLV-1. We thus hypothesized that the thymus might also be infected by the Zika virus (ZIKV). Herein we provide compelling evidence that ZIKV targets human thymic epithelial cells (TEC)
in vivo
and
in vitro
. ZIKV-infection enhances keratinization of TEC, with a decrease in proliferation and increase in cell death. Moreover, ZIKV modulates a high amount of coding RNAs with upregulation of genes related to cell adhesion and migration, as well as non-coding genes including miRNAs, circRNAs and lncRNAs. Moreover, we observed enhanced attachment of lymphoblastic T-cells to infected TEC, as well as virus transfer to those cells. Lastly, alterations in thymuses from babies congenitally infected were seen, with the presence of viral envelope protein in TEC. Taken together, our data reveals that the thymus, particularly the thymic epithelium, is a target for the ZIKV with changes in the expression of molecules that are relevant for interactions with developing thymocytes.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31992777</pmid><doi>10.1038/s41598-020-58135-y</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0003-3697-1506</orcidid><orcidid>https://orcid.org/0000-0002-0109-4809</orcidid><orcidid>https://orcid.org/0000-0002-2193-2224</orcidid><orcidid>https://orcid.org/0000-0002-2034-0294</orcidid><orcidid>https://orcid.org/0000-0001-9899-3386</orcidid><orcidid>https://orcid.org/0000-0001-7171-9977</orcidid><orcidid>https://orcid.org/0000-0001-5724-6106</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2020-01, Vol.10 (1), p.1378-1378, Article 1378 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6987159 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Springer Nature OA Free Journals; Nature Free; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 38/1 38/39 38/77 631/250/1620 631/250/255/2514 82/51 96/2 96/31 96/63 Animals Cell adhesion Cell adhesion & migration Cell death Cell migration Chlorocebus aethiops Epithelial cells Epithelial Cells - metabolism Epithelial Cells - pathology Epithelial Cells - virology Epithelium Epithelium - metabolism Epithelium - pathology Epithelium - virology HIV Human immunodeficiency virus Humanities and Social Sciences Humans Keratinization Lymphocytes T multidisciplinary Multidisciplinary Sciences RNA viruses Science Science & Technology Science & Technology - Other Topics Science (multidisciplinary) Thymocytes Thymocytes - metabolism Thymocytes - pathology Thymocytes - virology Thymus Thymus Gland - metabolism Thymus Gland - pathology Thymus Gland - virology Vector-borne diseases Vero Cells Viral envelope proteins Viral Tropism Zika virus Zika Virus - physiology Zika Virus Infection - metabolism Zika Virus Infection - pathology |
| title | Zika virus targets the human thymic epithelium |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T02%3A10%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_sprin&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Zika%20virus%20targets%20the%20human%20thymic%20epithelium&rft.jtitle=Scientific%20reports&rft.au=Messias,%20Carolina%20V.&rft.date=2020-01-28&rft.volume=10&rft.issue=1&rft.spage=1378&rft.epage=1378&rft.pages=1378-1378&rft.artnum=1378&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-020-58135-y&rft_dat=%3Cproquest_sprin%3E2348112879%3C/proquest_sprin%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2348112879&rft_id=info:pmid/31992777&rfr_iscdi=true |