Synergistic Activity of Fluoroquinolones Combining with Artesunate Against Multidrug-Resistant Escherichia coli
Multidrug resistance (MDR) is an increasing public health concern worldwide. Artesunate (ART) has been reported to be significantly effective in enhancing the effectiveness of various β-lactam antibiotics against MDR Escherichia coli via inhibiting the efflux pump genes. Apart from β-lactam antibiot...
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| Veröffentlicht in: | Microbial drug resistance (Larchmont, N.Y.) N.Y.), 2020-01, Vol.26 (1), p.81-88 |
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| creator | Wei, SiMin Yang, YueFei Tian, Wei Liu, MingJiang Yin, ShaoJie Li, JinGui |
| description | Multidrug resistance (MDR) is an increasing public health concern worldwide. Artesunate (ART) has been reported to be significantly effective in enhancing the effectiveness of various β-lactam antibiotics against MDR
Escherichia coli
via inhibiting the efflux pump genes. Apart from β-lactam antibiotics, there is no report regarding the potential synergistic effects of ART combining with fluoroquinolones (FQs). In this study, we investigated whether ART can enhance the antibacterial effects of FQs
in vitro
. The antibacterial activity of ART and antibiotics against 13 animal-derived
E. coli
clinical isolates was assessed for screening MDR strains. Then the synergistic activity of FQs with ART against MDR
E. coli
isolates was evaluated. Daunorubicin (DNR) accumulation within
E. coli
and messenger RNA (mRNA) expressions of
acrA
,
acrB
,
tolC
, and
qnr
genes were investigated. The results showed that ART did not show significant antimicrobial activity. However, a dramatically synergistic activity of ART combining with FQs was obsessed with (ΣFIC) = 0.12–0.33. ART increased the DNR accumulation and reduced acrAB-tolC mRNA expression, but enhanced the mRNA expression of
qnrS
and
qnrB
within MDR
E. coli
isolates. These findings suggest that ART can potentiate FQs activity which may be associated with drug accumulation by inhibiting the expression of acrAB-tolC. |
| doi_str_mv | 10.1089/mdr.2018.0463 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6978754</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2315970534</sourcerecordid><originalsourceid>FETCH-LOGICAL-c459t-ae4fc06542f3c6ae20077d4fddcb59e73090a7d8c8644ba0bd781209c66a1c713</originalsourceid><addsrcrecordid>eNqFkU1v1DAQhiNERUvhyBVZ4sIlix07_rggrVYtIBVVauFsOc4k68qxi-0U7b8n0ZYKuHDyyPPo1cw8VfWG4A3BUn2Y-rRpMJEbzDh9Vp0RRUktGZPPlxoLXvNGsdPqZc53GOOWcPqiOqVEUMmpOKvi7SFAGl0uzqKtLe7BlQOKA7r0c0zxx-xC9DFARrs4dS64MKKfruzRNhXIczAF0HY0LuSCvs6-uD7NY30DeUk0oaCLbPeQnN07g2z07lV1Mhif4fXje159v7z4tvtcX11_-rLbXtWWtarUBthgMW9ZM1DLDTQYC9Gzoe9t1yoQFCtsRC-t5Ix1Bne9kKTBynJuiBWEnlcfj7n3czdBbyGUZLy-T24y6aCjcfrvTnB7PcYHzZWQomVLwPvHgPUKkIueXLbgvQkQ56wbSlolcEtX9N0_6F2cU1jWWygqCWvaZp2oPlI2xZwTDE_DEKxXlXpRqVeVelW58G__3OCJ_u1uAegRWL9NCN5BB6n8J_YXKWSucg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2338142521</pqid></control><display><type>article</type><title>Synergistic Activity of Fluoroquinolones Combining with Artesunate Against Multidrug-Resistant Escherichia coli</title><source>Alma/SFX Local Collection</source><creator>Wei, SiMin ; Yang, YueFei ; Tian, Wei ; Liu, MingJiang ; Yin, ShaoJie ; Li, JinGui</creator><creatorcontrib>Wei, SiMin ; Yang, YueFei ; Tian, Wei ; Liu, MingJiang ; Yin, ShaoJie ; Li, JinGui</creatorcontrib><description>Multidrug resistance (MDR) is an increasing public health concern worldwide. Artesunate (ART) has been reported to be significantly effective in enhancing the effectiveness of various β-lactam antibiotics against MDR
Escherichia coli
via inhibiting the efflux pump genes. Apart from β-lactam antibiotics, there is no report regarding the potential synergistic effects of ART combining with fluoroquinolones (FQs). In this study, we investigated whether ART can enhance the antibacterial effects of FQs
in vitro
. The antibacterial activity of ART and antibiotics against 13 animal-derived
E. coli
clinical isolates was assessed for screening MDR strains. Then the synergistic activity of FQs with ART against MDR
E. coli
isolates was evaluated. Daunorubicin (DNR) accumulation within
E. coli
and messenger RNA (mRNA) expressions of
acrA
,
acrB
,
tolC
, and
qnr
genes were investigated. The results showed that ART did not show significant antimicrobial activity. However, a dramatically synergistic activity of ART combining with FQs was obsessed with (ΣFIC) = 0.12–0.33. ART increased the DNR accumulation and reduced acrAB-tolC mRNA expression, but enhanced the mRNA expression of
qnrS
and
qnrB
within MDR
E. coli
isolates. These findings suggest that ART can potentiate FQs activity which may be associated with drug accumulation by inhibiting the expression of acrAB-tolC.</description><identifier>ISSN: 1076-6294</identifier><identifier>EISSN: 1931-8448</identifier><identifier>DOI: 10.1089/mdr.2018.0463</identifier><identifier>PMID: 31738637</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc., publishers</publisher><subject>Accumulation ; Amides ; Antibacterial activity ; Antibiotics ; Antimicrobial activity ; Artesunate ; Clinical isolates ; Daunorubicin ; E coli ; Efflux ; Escherichia coli ; Fluoroquinolones ; Gene expression ; Genes ; Multidrug resistance ; Multidrug resistant organisms ; Public health ; Synergistic effect ; Veterinary Microbiology ; β-Lactam antibiotics</subject><ispartof>Microbial drug resistance (Larchmont, N.Y.), 2020-01, Vol.26 (1), p.81-88</ispartof><rights>SiMin Wei et al., 2019; Published by Mary Ann Liebert, Inc.</rights><rights>Copyright Mary Ann Liebert, Inc. Jan 2020</rights><rights>SiMin Wei et al., 2019; Published by Mary Ann Liebert, Inc. 2019 SiMin Wei et al.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-ae4fc06542f3c6ae20077d4fddcb59e73090a7d8c8644ba0bd781209c66a1c713</citedby><cites>FETCH-LOGICAL-c459t-ae4fc06542f3c6ae20077d4fddcb59e73090a7d8c8644ba0bd781209c66a1c713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31738637$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wei, SiMin</creatorcontrib><creatorcontrib>Yang, YueFei</creatorcontrib><creatorcontrib>Tian, Wei</creatorcontrib><creatorcontrib>Liu, MingJiang</creatorcontrib><creatorcontrib>Yin, ShaoJie</creatorcontrib><creatorcontrib>Li, JinGui</creatorcontrib><title>Synergistic Activity of Fluoroquinolones Combining with Artesunate Against Multidrug-Resistant Escherichia coli</title><title>Microbial drug resistance (Larchmont, N.Y.)</title><addtitle>Microb Drug Resist</addtitle><description>Multidrug resistance (MDR) is an increasing public health concern worldwide. Artesunate (ART) has been reported to be significantly effective in enhancing the effectiveness of various β-lactam antibiotics against MDR
Escherichia coli
via inhibiting the efflux pump genes. Apart from β-lactam antibiotics, there is no report regarding the potential synergistic effects of ART combining with fluoroquinolones (FQs). In this study, we investigated whether ART can enhance the antibacterial effects of FQs
in vitro
. The antibacterial activity of ART and antibiotics against 13 animal-derived
E. coli
clinical isolates was assessed for screening MDR strains. Then the synergistic activity of FQs with ART against MDR
E. coli
isolates was evaluated. Daunorubicin (DNR) accumulation within
E. coli
and messenger RNA (mRNA) expressions of
acrA
,
acrB
,
tolC
, and
qnr
genes were investigated. The results showed that ART did not show significant antimicrobial activity. However, a dramatically synergistic activity of ART combining with FQs was obsessed with (ΣFIC) = 0.12–0.33. ART increased the DNR accumulation and reduced acrAB-tolC mRNA expression, but enhanced the mRNA expression of
qnrS
and
qnrB
within MDR
E. coli
isolates. These findings suggest that ART can potentiate FQs activity which may be associated with drug accumulation by inhibiting the expression of acrAB-tolC.</description><subject>Accumulation</subject><subject>Amides</subject><subject>Antibacterial activity</subject><subject>Antibiotics</subject><subject>Antimicrobial activity</subject><subject>Artesunate</subject><subject>Clinical isolates</subject><subject>Daunorubicin</subject><subject>E coli</subject><subject>Efflux</subject><subject>Escherichia coli</subject><subject>Fluoroquinolones</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Multidrug resistance</subject><subject>Multidrug resistant organisms</subject><subject>Public health</subject><subject>Synergistic effect</subject><subject>Veterinary Microbiology</subject><subject>β-Lactam antibiotics</subject><issn>1076-6294</issn><issn>1931-8448</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>1-M</sourceid><recordid>eNqFkU1v1DAQhiNERUvhyBVZ4sIlix07_rggrVYtIBVVauFsOc4k68qxi-0U7b8n0ZYKuHDyyPPo1cw8VfWG4A3BUn2Y-rRpMJEbzDh9Vp0RRUktGZPPlxoLXvNGsdPqZc53GOOWcPqiOqVEUMmpOKvi7SFAGl0uzqKtLe7BlQOKA7r0c0zxx-xC9DFARrs4dS64MKKfruzRNhXIczAF0HY0LuSCvs6-uD7NY30DeUk0oaCLbPeQnN07g2z07lV1Mhif4fXje159v7z4tvtcX11_-rLbXtWWtarUBthgMW9ZM1DLDTQYC9Gzoe9t1yoQFCtsRC-t5Ix1Bne9kKTBynJuiBWEnlcfj7n3czdBbyGUZLy-T24y6aCjcfrvTnB7PcYHzZWQomVLwPvHgPUKkIueXLbgvQkQ56wbSlolcEtX9N0_6F2cU1jWWygqCWvaZp2oPlI2xZwTDE_DEKxXlXpRqVeVelW58G__3OCJ_u1uAegRWL9NCN5BB6n8J_YXKWSucg</recordid><startdate>20200101</startdate><enddate>20200101</enddate><creator>Wei, SiMin</creator><creator>Yang, YueFei</creator><creator>Tian, Wei</creator><creator>Liu, MingJiang</creator><creator>Yin, ShaoJie</creator><creator>Li, JinGui</creator><general>Mary Ann Liebert, Inc., publishers</general><general>Mary Ann Liebert, Inc</general><scope>1-M</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200101</creationdate><title>Synergistic Activity of Fluoroquinolones Combining with Artesunate Against Multidrug-Resistant Escherichia coli</title><author>Wei, SiMin ; Yang, YueFei ; Tian, Wei ; Liu, MingJiang ; Yin, ShaoJie ; Li, JinGui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-ae4fc06542f3c6ae20077d4fddcb59e73090a7d8c8644ba0bd781209c66a1c713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Accumulation</topic><topic>Amides</topic><topic>Antibacterial activity</topic><topic>Antibiotics</topic><topic>Antimicrobial activity</topic><topic>Artesunate</topic><topic>Clinical isolates</topic><topic>Daunorubicin</topic><topic>E coli</topic><topic>Efflux</topic><topic>Escherichia coli</topic><topic>Fluoroquinolones</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Multidrug resistance</topic><topic>Multidrug resistant organisms</topic><topic>Public health</topic><topic>Synergistic effect</topic><topic>Veterinary Microbiology</topic><topic>β-Lactam antibiotics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wei, SiMin</creatorcontrib><creatorcontrib>Yang, YueFei</creatorcontrib><creatorcontrib>Tian, Wei</creatorcontrib><creatorcontrib>Liu, MingJiang</creatorcontrib><creatorcontrib>Yin, ShaoJie</creatorcontrib><creatorcontrib>Li, JinGui</creatorcontrib><collection>Mary Ann Liebert Online - Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Microbial drug resistance (Larchmont, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wei, SiMin</au><au>Yang, YueFei</au><au>Tian, Wei</au><au>Liu, MingJiang</au><au>Yin, ShaoJie</au><au>Li, JinGui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synergistic Activity of Fluoroquinolones Combining with Artesunate Against Multidrug-Resistant Escherichia coli</atitle><jtitle>Microbial drug resistance (Larchmont, N.Y.)</jtitle><addtitle>Microb Drug Resist</addtitle><date>2020-01-01</date><risdate>2020</risdate><volume>26</volume><issue>1</issue><spage>81</spage><epage>88</epage><pages>81-88</pages><issn>1076-6294</issn><eissn>1931-8448</eissn><abstract>Multidrug resistance (MDR) is an increasing public health concern worldwide. Artesunate (ART) has been reported to be significantly effective in enhancing the effectiveness of various β-lactam antibiotics against MDR
Escherichia coli
via inhibiting the efflux pump genes. Apart from β-lactam antibiotics, there is no report regarding the potential synergistic effects of ART combining with fluoroquinolones (FQs). In this study, we investigated whether ART can enhance the antibacterial effects of FQs
in vitro
. The antibacterial activity of ART and antibiotics against 13 animal-derived
E. coli
clinical isolates was assessed for screening MDR strains. Then the synergistic activity of FQs with ART against MDR
E. coli
isolates was evaluated. Daunorubicin (DNR) accumulation within
E. coli
and messenger RNA (mRNA) expressions of
acrA
,
acrB
,
tolC
, and
qnr
genes were investigated. The results showed that ART did not show significant antimicrobial activity. However, a dramatically synergistic activity of ART combining with FQs was obsessed with (ΣFIC) = 0.12–0.33. ART increased the DNR accumulation and reduced acrAB-tolC mRNA expression, but enhanced the mRNA expression of
qnrS
and
qnrB
within MDR
E. coli
isolates. These findings suggest that ART can potentiate FQs activity which may be associated with drug accumulation by inhibiting the expression of acrAB-tolC.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc., publishers</pub><pmid>31738637</pmid><doi>10.1089/mdr.2018.0463</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Microbial drug resistance (Larchmont, N.Y.), 2020-01, Vol.26 (1), p.81-88 |
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| source | Alma/SFX Local Collection |
| subjects | Accumulation Amides Antibacterial activity Antibiotics Antimicrobial activity Artesunate Clinical isolates Daunorubicin E coli Efflux Escherichia coli Fluoroquinolones Gene expression Genes Multidrug resistance Multidrug resistant organisms Public health Synergistic effect Veterinary Microbiology β-Lactam antibiotics |
| title | Synergistic Activity of Fluoroquinolones Combining with Artesunate Against Multidrug-Resistant Escherichia coli |
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