Laminin is the ECM niche for trophoblast stem cells
The niche is a specialized microenvironment for tissue stem cells in vivo. It has long been emphasized that niche ECM molecules act on tissue stem cells to regulate their behavior, but the molecular entities of these interactions remain to be fully elucidated. Here, we report that laminin forms the...
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| creator | Kiyozumi, Daiji Nakano, Itsuko Sato-Nishiuchi, Ryoko Tanaka, Satoshi Sekiguchi, Kiyotoshi |
| description | The niche is a specialized microenvironment for tissue stem cells in vivo. It has long been emphasized that niche ECM molecules act on tissue stem cells to regulate their behavior, but the molecular entities of these interactions remain to be fully elucidated. Here, we report that laminin forms the in vivo ECM niche for trophoblast stem cells (TSCs), the tissue stem cells of the placenta. TSCs expressed fibronectin-binding, vitronectin-binding, and laminin-binding integrins, whereas the integrin ligands present in the TSC niche were collagen and laminin. Therefore, the only niche integrin ligand available for TSCs in vivo was laminin. Laminin promoted TSC adhesion and proliferation in vitro in an integrin binding-dependent manner. Importantly, when the integrin-binding ability of laminin was genetically ablated in mice, the size of the TSC population was significantly reduced compared with that in control mice. The present findings underscore an ECM niche function of laminin to support tissue stem cell maintenance in vivo. |
| doi_str_mv | 10.26508/lsa.201900515 |
| format | Article |
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It has long been emphasized that niche ECM molecules act on tissue stem cells to regulate their behavior, but the molecular entities of these interactions remain to be fully elucidated. Here, we report that laminin forms the in vivo ECM niche for trophoblast stem cells (TSCs), the tissue stem cells of the placenta. TSCs expressed fibronectin-binding, vitronectin-binding, and laminin-binding integrins, whereas the integrin ligands present in the TSC niche were collagen and laminin. Therefore, the only niche integrin ligand available for TSCs in vivo was laminin. Laminin promoted TSC adhesion and proliferation in vitro in an integrin binding-dependent manner. Importantly, when the integrin-binding ability of laminin was genetically ablated in mice, the size of the TSC population was significantly reduced compared with that in control mice. The present findings underscore an ECM niche function of laminin to support tissue stem cell maintenance in vivo.</description><identifier>ISSN: 2575-1077</identifier><identifier>EISSN: 2575-1077</identifier><identifier>DOI: 10.26508/lsa.201900515</identifier><identifier>PMID: 31937556</identifier><language>eng</language><publisher>United States: Life Science Alliance LLC</publisher><subject>Animals ; Blastocyst - cytology ; Cell Adhesion - genetics ; Cell Proliferation - genetics ; Cells, Cultured ; Collagen - metabolism ; Extracellular Matrix - metabolism ; Female ; Gene Knock-In Techniques ; Integrins - metabolism ; Laminin - genetics ; Laminin - metabolism ; Mice ; Mice, Transgenic ; Pregnancy ; Stem Cell Niche - genetics ; Stem Cells - metabolism ; Trophoblasts - metabolism</subject><ispartof>Life science alliance, 2020-02, Vol.3 (2), p.e201900515</ispartof><rights>2020 Kiyozumi et al.</rights><rights>2020 Kiyozumi et al. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-ac1c635ba5d4bfdaa92785a873323d7d95878a8c637c8c408f4eed6df5058e293</citedby><cites>FETCH-LOGICAL-c456t-ac1c635ba5d4bfdaa92785a873323d7d95878a8c637c8c408f4eed6df5058e293</cites><orcidid>0000-0003-3289-227X ; 0000-0002-6433-1410</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977391/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977391/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31937556$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kiyozumi, Daiji</creatorcontrib><creatorcontrib>Nakano, Itsuko</creatorcontrib><creatorcontrib>Sato-Nishiuchi, Ryoko</creatorcontrib><creatorcontrib>Tanaka, Satoshi</creatorcontrib><creatorcontrib>Sekiguchi, Kiyotoshi</creatorcontrib><title>Laminin is the ECM niche for trophoblast stem cells</title><title>Life science alliance</title><addtitle>Life Sci Alliance</addtitle><description>The niche is a specialized microenvironment for tissue stem cells in vivo. It has long been emphasized that niche ECM molecules act on tissue stem cells to regulate their behavior, but the molecular entities of these interactions remain to be fully elucidated. Here, we report that laminin forms the in vivo ECM niche for trophoblast stem cells (TSCs), the tissue stem cells of the placenta. TSCs expressed fibronectin-binding, vitronectin-binding, and laminin-binding integrins, whereas the integrin ligands present in the TSC niche were collagen and laminin. Therefore, the only niche integrin ligand available for TSCs in vivo was laminin. Laminin promoted TSC adhesion and proliferation in vitro in an integrin binding-dependent manner. Importantly, when the integrin-binding ability of laminin was genetically ablated in mice, the size of the TSC population was significantly reduced compared with that in control mice. The present findings underscore an ECM niche function of laminin to support tissue stem cell maintenance in vivo.</description><subject>Animals</subject><subject>Blastocyst - cytology</subject><subject>Cell Adhesion - genetics</subject><subject>Cell Proliferation - genetics</subject><subject>Cells, Cultured</subject><subject>Collagen - metabolism</subject><subject>Extracellular Matrix - metabolism</subject><subject>Female</subject><subject>Gene Knock-In Techniques</subject><subject>Integrins - metabolism</subject><subject>Laminin - genetics</subject><subject>Laminin - metabolism</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Pregnancy</subject><subject>Stem Cell Niche - genetics</subject><subject>Stem Cells - metabolism</subject><subject>Trophoblasts - metabolism</subject><issn>2575-1077</issn><issn>2575-1077</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkE1LAzEQhoMottRePUqOXrbmY2eTXAQprQoVL3oO2WzWRnY3NdkK_ntXW0uFgRmYZ96ZeRG6pGTGCiDypklmxghVhACFEzRmICCjRIjTo3qEpim9E0LYEDnk52jEqeICoBgjvjKt73yHfcL92uHF_Al33g5VHSLuY9isQ9mY1OPUuxZb1zTpAp3Vpkluus8T9LpcvMwfstXz_eP8bpXZHIo-M5bagkNpoMrLujJGMSHBSME545WoFEghjRwYYaXNiaxz56qiqoGAdEzxCbrd6W62Zesq67o-mkZvom9N_NLBeP2_0_m1fgufulBCcEUHgeu9QAwfW5d63fr084LpXNgmzTiXSgpSsAGd7VAbQ0rR1Yc1lOhfs_Vgtj6YPQxcHR93wP-s5d_Ckno_</recordid><startdate>20200201</startdate><enddate>20200201</enddate><creator>Kiyozumi, Daiji</creator><creator>Nakano, Itsuko</creator><creator>Sato-Nishiuchi, Ryoko</creator><creator>Tanaka, Satoshi</creator><creator>Sekiguchi, Kiyotoshi</creator><general>Life Science Alliance LLC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3289-227X</orcidid><orcidid>https://orcid.org/0000-0002-6433-1410</orcidid></search><sort><creationdate>20200201</creationdate><title>Laminin is the ECM niche for trophoblast stem cells</title><author>Kiyozumi, Daiji ; Nakano, Itsuko ; Sato-Nishiuchi, Ryoko ; Tanaka, Satoshi ; Sekiguchi, Kiyotoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-ac1c635ba5d4bfdaa92785a873323d7d95878a8c637c8c408f4eed6df5058e293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Blastocyst - cytology</topic><topic>Cell Adhesion - genetics</topic><topic>Cell Proliferation - genetics</topic><topic>Cells, Cultured</topic><topic>Collagen - metabolism</topic><topic>Extracellular Matrix - metabolism</topic><topic>Female</topic><topic>Gene Knock-In Techniques</topic><topic>Integrins - metabolism</topic><topic>Laminin - genetics</topic><topic>Laminin - metabolism</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Pregnancy</topic><topic>Stem Cell Niche - genetics</topic><topic>Stem Cells - metabolism</topic><topic>Trophoblasts - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kiyozumi, Daiji</creatorcontrib><creatorcontrib>Nakano, Itsuko</creatorcontrib><creatorcontrib>Sato-Nishiuchi, Ryoko</creatorcontrib><creatorcontrib>Tanaka, Satoshi</creatorcontrib><creatorcontrib>Sekiguchi, Kiyotoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Life science alliance</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kiyozumi, Daiji</au><au>Nakano, Itsuko</au><au>Sato-Nishiuchi, Ryoko</au><au>Tanaka, Satoshi</au><au>Sekiguchi, Kiyotoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Laminin is the ECM niche for trophoblast stem cells</atitle><jtitle>Life science alliance</jtitle><addtitle>Life Sci Alliance</addtitle><date>2020-02-01</date><risdate>2020</risdate><volume>3</volume><issue>2</issue><spage>e201900515</spage><pages>e201900515-</pages><issn>2575-1077</issn><eissn>2575-1077</eissn><abstract>The niche is a specialized microenvironment for tissue stem cells in vivo. It has long been emphasized that niche ECM molecules act on tissue stem cells to regulate their behavior, but the molecular entities of these interactions remain to be fully elucidated. Here, we report that laminin forms the in vivo ECM niche for trophoblast stem cells (TSCs), the tissue stem cells of the placenta. TSCs expressed fibronectin-binding, vitronectin-binding, and laminin-binding integrins, whereas the integrin ligands present in the TSC niche were collagen and laminin. Therefore, the only niche integrin ligand available for TSCs in vivo was laminin. Laminin promoted TSC adhesion and proliferation in vitro in an integrin binding-dependent manner. Importantly, when the integrin-binding ability of laminin was genetically ablated in mice, the size of the TSC population was significantly reduced compared with that in control mice. 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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Animals Blastocyst - cytology Cell Adhesion - genetics Cell Proliferation - genetics Cells, Cultured Collagen - metabolism Extracellular Matrix - metabolism Female Gene Knock-In Techniques Integrins - metabolism Laminin - genetics Laminin - metabolism Mice Mice, Transgenic Pregnancy Stem Cell Niche - genetics Stem Cells - metabolism Trophoblasts - metabolism |
| title | Laminin is the ECM niche for trophoblast stem cells |
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