Isolation and phenotypic characterization of tumor cells of patients with a diagnosis of ovarian cancer
Ovarian cancer is the fifth leading cause of cancer‐related deaths. It causes approximately 125,000 deaths per year worldwide; its diagnosis is made in advanced stages resulting in a high mortality rate. The objective of the study was optimizing the isolation of cells obtained from the solid tumor a...
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| Veröffentlicht in: | Journal of cellular physiology 2020-04, Vol.235 (4), p.3320-3328 |
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| creator | Gutiérrez‐Castañeda, Luz D. Tovar‐Parra, David Quintero, Gloria Amezquita, Lorena Guerrero, Carlos Sanabria, Daniel |
| description | Ovarian cancer is the fifth leading cause of cancer‐related deaths. It causes approximately 125,000 deaths per year worldwide; its diagnosis is made in advanced stages resulting in a high mortality rate. The objective of the study was optimizing the isolation of cells obtained from the solid tumor and ascitic fluid of patients with ovarian cancer and the phenotype with markers related to the epithelial–mesenchymal transition. For this, the solid tumor tissue was disaggregated and cultivated with different methodologies. As a result, cell growth was obtained and epi‐immunofluorescence was performed using antibodies against E‐cadherin, EpCAM, N‐cadherin, vimentin, CD133, and CD44. The primary culture from the solid tumor was obtained using Dispase II and DMEM/F12. Finally, heterogeneity was detected in terms of the expression of mesenchymal and epithelial type markers in the two types of isolated cells. Additionally, CD133 and CD44 expression was detected, proteins associated with the tumor stem cells phenotype.
The ovarian cancer cells isolated were heterogeneous. This was detected in terms of expression of mesenchymal and epithelial type markers. Additionally, CD133 and CD44 expression was detected, proteins associated with the tumor stem cells phenotype. |
| doi_str_mv | 10.1002/jcp.29220 |
| format | Article |
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The ovarian cancer cells isolated were heterogeneous. This was detected in terms of expression of mesenchymal and epithelial type markers. Additionally, CD133 and CD44 expression was detected, proteins associated with the tumor stem cells phenotype.</description><identifier>ISSN: 0021-9541</identifier><identifier>EISSN: 1097-4652</identifier><identifier>DOI: 10.1002/jcp.29220</identifier><identifier>PMID: 31549393</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Antibodies ; Ascitic fluid ; Biomarkers, Tumor - analysis ; Cadherins - metabolism ; Cancer ; CD133 ; CD44 ; CD44 antigen ; Cell culture ; Cell Line, Tumor ; Cell Proliferation - physiology ; Diagnosis ; epithelial ; Epithelial-Mesenchymal Transition - genetics ; Fatalities ; Female ; Heterogeneity ; Humans ; Immunofluorescence ; Markers ; mesenchymal ; Mesenchyme ; Neoplastic Stem Cells - pathology ; Original ; Original s ; Ovarian cancer ; Ovarian Neoplasms - diagnosis ; Ovarian Neoplasms - pathology ; Phenotypes ; Solid tumors ; Stem cells ; tumor ; Tumor cells ; Tumors ; Vimentin ; Vimentin - metabolism</subject><ispartof>Journal of cellular physiology, 2020-04, Vol.235 (4), p.3320-3328</ispartof><rights>2019 The Authors. Published by Wiley Periodicals, Inc.</rights><rights>2019 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals, Inc.</rights><rights>2019. This article is published under http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4430-c643c16eac069a4ebe1c4639b485f687a0abef4e3e6f2cac55d9041e980896043</citedby><cites>FETCH-LOGICAL-c4430-c643c16eac069a4ebe1c4639b485f687a0abef4e3e6f2cac55d9041e980896043</cites><orcidid>0000-0003-2863-6345 ; 0000-0002-6155-3771 ; 0000-0002-1005-4526 ; 0000-0002-2258-6546 ; 0000-0003-4675-8688 ; 0000-0001-5591-2531</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcp.29220$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcp.29220$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31549393$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gutiérrez‐Castañeda, Luz D.</creatorcontrib><creatorcontrib>Tovar‐Parra, David</creatorcontrib><creatorcontrib>Quintero, Gloria</creatorcontrib><creatorcontrib>Amezquita, Lorena</creatorcontrib><creatorcontrib>Guerrero, Carlos</creatorcontrib><creatorcontrib>Sanabria, Daniel</creatorcontrib><title>Isolation and phenotypic characterization of tumor cells of patients with a diagnosis of ovarian cancer</title><title>Journal of cellular physiology</title><addtitle>J Cell Physiol</addtitle><description>Ovarian cancer is the fifth leading cause of cancer‐related deaths. It causes approximately 125,000 deaths per year worldwide; its diagnosis is made in advanced stages resulting in a high mortality rate. The objective of the study was optimizing the isolation of cells obtained from the solid tumor and ascitic fluid of patients with ovarian cancer and the phenotype with markers related to the epithelial–mesenchymal transition. For this, the solid tumor tissue was disaggregated and cultivated with different methodologies. As a result, cell growth was obtained and epi‐immunofluorescence was performed using antibodies against E‐cadherin, EpCAM, N‐cadherin, vimentin, CD133, and CD44. The primary culture from the solid tumor was obtained using Dispase II and DMEM/F12. Finally, heterogeneity was detected in terms of the expression of mesenchymal and epithelial type markers in the two types of isolated cells. Additionally, CD133 and CD44 expression was detected, proteins associated with the tumor stem cells phenotype.
The ovarian cancer cells isolated were heterogeneous. This was detected in terms of expression of mesenchymal and epithelial type markers. Additionally, CD133 and CD44 expression was detected, proteins associated with the tumor stem cells phenotype.</description><subject>Antibodies</subject><subject>Ascitic fluid</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Cadherins - metabolism</subject><subject>Cancer</subject><subject>CD133</subject><subject>CD44</subject><subject>CD44 antigen</subject><subject>Cell culture</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - physiology</subject><subject>Diagnosis</subject><subject>epithelial</subject><subject>Epithelial-Mesenchymal Transition - genetics</subject><subject>Fatalities</subject><subject>Female</subject><subject>Heterogeneity</subject><subject>Humans</subject><subject>Immunofluorescence</subject><subject>Markers</subject><subject>mesenchymal</subject><subject>Mesenchyme</subject><subject>Neoplastic Stem Cells - pathology</subject><subject>Original</subject><subject>Original s</subject><subject>Ovarian cancer</subject><subject>Ovarian Neoplasms - diagnosis</subject><subject>Ovarian Neoplasms - pathology</subject><subject>Phenotypes</subject><subject>Solid tumors</subject><subject>Stem cells</subject><subject>tumor</subject><subject>Tumor cells</subject><subject>Tumors</subject><subject>Vimentin</subject><subject>Vimentin - metabolism</subject><issn>0021-9541</issn><issn>1097-4652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNp1kc1rGzEQxUVpaFy3h_4DRdBLe9hEXyuvLoVg-pEQSA_tWYzlWVtmLW2l3QT3r6-cTUNbyGkY3o83b3iEvOHsjDMmzneuPxNGCPaMzDgzi0rpWjwns6LxytSKn5KXOe8YY8ZI-YKcSl4rI42ckc1ljh0MPgYKYU37LYY4HHrvqNtCAjdg8r8mPbZ0GPcxUYddl49rXwQMQ6Z3fthSoGsPmxCzvxfjLSQPgToIDtMrctJCl_H1w5yTH58_fV9-ra5vvlwuL64rp5RkldNKOq4RHNMGFK6QO6WlWammbnWzAAYrbBVK1K1w4Op6bZjiaBrWGM2UnJOPk28_rva4diVegs72ye8hHWwEb_9Vgt_aTby12iykWDTF4P2DQYo_R8yD3ft8_BgCxjFbIYzWtWbieOvdf-gujimU96yQUtZaNmXOyYeJcinmnLB9DMOZPdZnS332vr7Cvv07_SP5p68CnE_Ane_w8LSTvVp-myx_A0Xwph0</recordid><startdate>202004</startdate><enddate>202004</enddate><creator>Gutiérrez‐Castañeda, Luz D.</creator><creator>Tovar‐Parra, David</creator><creator>Quintero, Gloria</creator><creator>Amezquita, Lorena</creator><creator>Guerrero, Carlos</creator><creator>Sanabria, Daniel</creator><general>Wiley Subscription Services, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-2863-6345</orcidid><orcidid>https://orcid.org/0000-0002-6155-3771</orcidid><orcidid>https://orcid.org/0000-0002-1005-4526</orcidid><orcidid>https://orcid.org/0000-0002-2258-6546</orcidid><orcidid>https://orcid.org/0000-0003-4675-8688</orcidid><orcidid>https://orcid.org/0000-0001-5591-2531</orcidid></search><sort><creationdate>202004</creationdate><title>Isolation and phenotypic characterization of tumor cells of patients with a diagnosis of ovarian cancer</title><author>Gutiérrez‐Castañeda, Luz D. ; Tovar‐Parra, David ; Quintero, Gloria ; Amezquita, Lorena ; Guerrero, Carlos ; Sanabria, Daniel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4430-c643c16eac069a4ebe1c4639b485f687a0abef4e3e6f2cac55d9041e980896043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antibodies</topic><topic>Ascitic fluid</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Cadherins - metabolism</topic><topic>Cancer</topic><topic>CD133</topic><topic>CD44</topic><topic>CD44 antigen</topic><topic>Cell culture</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - physiology</topic><topic>Diagnosis</topic><topic>epithelial</topic><topic>Epithelial-Mesenchymal Transition - genetics</topic><topic>Fatalities</topic><topic>Female</topic><topic>Heterogeneity</topic><topic>Humans</topic><topic>Immunofluorescence</topic><topic>Markers</topic><topic>mesenchymal</topic><topic>Mesenchyme</topic><topic>Neoplastic Stem Cells - pathology</topic><topic>Original</topic><topic>Original s</topic><topic>Ovarian cancer</topic><topic>Ovarian Neoplasms - diagnosis</topic><topic>Ovarian Neoplasms - pathology</topic><topic>Phenotypes</topic><topic>Solid tumors</topic><topic>Stem cells</topic><topic>tumor</topic><topic>Tumor cells</topic><topic>Tumors</topic><topic>Vimentin</topic><topic>Vimentin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gutiérrez‐Castañeda, Luz D.</creatorcontrib><creatorcontrib>Tovar‐Parra, David</creatorcontrib><creatorcontrib>Quintero, Gloria</creatorcontrib><creatorcontrib>Amezquita, Lorena</creatorcontrib><creatorcontrib>Guerrero, Carlos</creatorcontrib><creatorcontrib>Sanabria, Daniel</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Wiley Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of cellular physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gutiérrez‐Castañeda, Luz D.</au><au>Tovar‐Parra, David</au><au>Quintero, Gloria</au><au>Amezquita, Lorena</au><au>Guerrero, Carlos</au><au>Sanabria, Daniel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and phenotypic characterization of tumor cells of patients with a diagnosis of ovarian cancer</atitle><jtitle>Journal of cellular physiology</jtitle><addtitle>J Cell Physiol</addtitle><date>2020-04</date><risdate>2020</risdate><volume>235</volume><issue>4</issue><spage>3320</spage><epage>3328</epage><pages>3320-3328</pages><issn>0021-9541</issn><eissn>1097-4652</eissn><abstract>Ovarian cancer is the fifth leading cause of cancer‐related deaths. It causes approximately 125,000 deaths per year worldwide; its diagnosis is made in advanced stages resulting in a high mortality rate. The objective of the study was optimizing the isolation of cells obtained from the solid tumor and ascitic fluid of patients with ovarian cancer and the phenotype with markers related to the epithelial–mesenchymal transition. For this, the solid tumor tissue was disaggregated and cultivated with different methodologies. As a result, cell growth was obtained and epi‐immunofluorescence was performed using antibodies against E‐cadherin, EpCAM, N‐cadherin, vimentin, CD133, and CD44. The primary culture from the solid tumor was obtained using Dispase II and DMEM/F12. Finally, heterogeneity was detected in terms of the expression of mesenchymal and epithelial type markers in the two types of isolated cells. Additionally, CD133 and CD44 expression was detected, proteins associated with the tumor stem cells phenotype.
The ovarian cancer cells isolated were heterogeneous. This was detected in terms of expression of mesenchymal and epithelial type markers. Additionally, CD133 and CD44 expression was detected, proteins associated with the tumor stem cells phenotype.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31549393</pmid><doi>10.1002/jcp.29220</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-2863-6345</orcidid><orcidid>https://orcid.org/0000-0002-6155-3771</orcidid><orcidid>https://orcid.org/0000-0002-1005-4526</orcidid><orcidid>https://orcid.org/0000-0002-2258-6546</orcidid><orcidid>https://orcid.org/0000-0003-4675-8688</orcidid><orcidid>https://orcid.org/0000-0001-5591-2531</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Antibodies Ascitic fluid Biomarkers, Tumor - analysis Cadherins - metabolism Cancer CD133 CD44 CD44 antigen Cell culture Cell Line, Tumor Cell Proliferation - physiology Diagnosis epithelial Epithelial-Mesenchymal Transition - genetics Fatalities Female Heterogeneity Humans Immunofluorescence Markers mesenchymal Mesenchyme Neoplastic Stem Cells - pathology Original Original s Ovarian cancer Ovarian Neoplasms - diagnosis Ovarian Neoplasms - pathology Phenotypes Solid tumors Stem cells tumor Tumor cells Tumors Vimentin Vimentin - metabolism |
| title | Isolation and phenotypic characterization of tumor cells of patients with a diagnosis of ovarian cancer |
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