Immobilization of Lipozyme TL 100L for methyl esterification of soybean oil deodorizer distillate
An immobilization method for binding cross-linked enzyme aggregates of Lipozyme TL 100L on macroporous resin NKA (CLEA-TLL@NKA) was developed in this study. The esterification activity of CLEA-TLL@NKA reached 6.4 U/mg. The surface structure of immobilized lipase was characterized by scanning electro...
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| Veröffentlicht in: | 3 Biotech 2020-02, Vol.10 (2), p.51-51, Article 51 |
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| creator | Zheng, Jianyong Wei, Wei Wang, Shengfan Li, Xiaojun Zhang, Yinjun Wang, Zhao |
| description | An immobilization method for binding cross-linked enzyme aggregates of Lipozyme TL 100L on macroporous resin NKA (CLEA-TLL@NKA) was developed in this study. The esterification activity of CLEA-TLL@NKA reached 6.4 U/mg. The surface structure of immobilized lipase was characterized by scanning electron microscopy. Methyl esterification reaction of soybean oil deodorizer distillate (SODD) was catalyzed by CLEA-TLL@NKA, which the conversion rate reached 98% and its activity retained over 90% after 20 batches of reaction. Compared with the commercial enzyme Lipozyme TLIM, half-life (
t
1/2
) of CLEA-TLL@NKA increased by 25 times and the catalytic activity increased by approximate 10 times. Thus, CLEA-TLL@NKA had high catalytic activity, good operational stability, and potential industrial application in the field of oil processing. |
| doi_str_mv | 10.1007/s13205-019-2028-6 |
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t
1/2
) of CLEA-TLL@NKA increased by 25 times and the catalytic activity increased by approximate 10 times. Thus, CLEA-TLL@NKA had high catalytic activity, good operational stability, and potential industrial application in the field of oil processing.</description><identifier>ISSN: 2190-572X</identifier><identifier>EISSN: 2190-5738</identifier><identifier>DOI: 10.1007/s13205-019-2028-6</identifier><identifier>PMID: 32002342</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Agriculture ; Bioinformatics ; Biomaterials ; Biotechnology ; Cancer Research ; Catalytic activity ; Chemistry ; Chemistry and Materials Science ; Crosslinking ; Distillates ; Enzymes ; Esterification ; half life ; Immobilization ; Industrial applications ; Lipase ; Original ; Original Article ; porous media ; Scanning electron microscopy ; Soybean oil ; Soybeans ; Stem Cells ; Surface structure ; triacylglycerol lipase ; Vegetable oils</subject><ispartof>3 Biotech, 2020-02, Vol.10 (2), p.51-51, Article 51</ispartof><rights>King Abdulaziz City for Science and Technology 2020</rights><rights>King Abdulaziz City for Science and Technology 2020.</rights><rights>2020© King Abdulaziz City for Science and Technology 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-13cc94a966332434162b1f6a4e477c7d1a154f38f18ae88eb00b7d31e40494313</citedby><cites>FETCH-LOGICAL-c503t-13cc94a966332434162b1f6a4e477c7d1a154f38f18ae88eb00b7d31e40494313</cites><orcidid>0000-0002-9821-1062 ; 0000-0002-9199-0059</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6969875/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6969875/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41464,42533,51294,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32002342$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zheng, Jianyong</creatorcontrib><creatorcontrib>Wei, Wei</creatorcontrib><creatorcontrib>Wang, Shengfan</creatorcontrib><creatorcontrib>Li, Xiaojun</creatorcontrib><creatorcontrib>Zhang, Yinjun</creatorcontrib><creatorcontrib>Wang, Zhao</creatorcontrib><title>Immobilization of Lipozyme TL 100L for methyl esterification of soybean oil deodorizer distillate</title><title>3 Biotech</title><addtitle>3 Biotech</addtitle><addtitle>3 Biotech</addtitle><description>An immobilization method for binding cross-linked enzyme aggregates of Lipozyme TL 100L on macroporous resin NKA (CLEA-TLL@NKA) was developed in this study. The esterification activity of CLEA-TLL@NKA reached 6.4 U/mg. The surface structure of immobilized lipase was characterized by scanning electron microscopy. Methyl esterification reaction of soybean oil deodorizer distillate (SODD) was catalyzed by CLEA-TLL@NKA, which the conversion rate reached 98% and its activity retained over 90% after 20 batches of reaction. Compared with the commercial enzyme Lipozyme TLIM, half-life (
t
1/2
) of CLEA-TLL@NKA increased by 25 times and the catalytic activity increased by approximate 10 times. Thus, CLEA-TLL@NKA had high catalytic activity, good operational stability, and potential industrial application in the field of oil processing.</description><subject>Agriculture</subject><subject>Bioinformatics</subject><subject>Biomaterials</subject><subject>Biotechnology</subject><subject>Cancer Research</subject><subject>Catalytic activity</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Crosslinking</subject><subject>Distillates</subject><subject>Enzymes</subject><subject>Esterification</subject><subject>half life</subject><subject>Immobilization</subject><subject>Industrial applications</subject><subject>Lipase</subject><subject>Original</subject><subject>Original Article</subject><subject>porous media</subject><subject>Scanning electron microscopy</subject><subject>Soybean oil</subject><subject>Soybeans</subject><subject>Stem Cells</subject><subject>Surface structure</subject><subject>triacylglycerol lipase</subject><subject>Vegetable oils</subject><issn>2190-572X</issn><issn>2190-5738</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqNkU2LFDEQhoMo7rLuD_AiAS9eWquSdCe5CLL4sdDgZQVvId1dvZuluzMmPcLMrzfDrOMHCOaSgnrqrbx5GXuO8BoB9JuMUkBdAdpKgDBV84idC7RQ1Vqax6dafD1jlznfQzk11hbhKTsrkyCkEufMX89z7MIU9n4NceFx5G3YxP1uJn7T8rKp5WNMfKb1bjdxyiulMIb-ROe468iXMkx8oDjEFPaU-BDyGqbJr_SMPRn9lOny4b5gXz68v7n6VLWfP15fvWurvga5Vij73ipvm0ZKoaTCRnQ4Nl6R0rrXA3qs1SjNiMaTMdQBdHqQSAqUVRLlBXt71N1su5mGnpY1-cltUph92rnog_uzs4Q7dxu_u8Y21ui6CLx6EEjx27Y4dXPIPRUTC8VtdkIaow2iEv-B1gAW0TYFffkXeh-3aSk_USglhdZFt1B4pPoUc040nt6N4A5xu2PcrsTtDnG7g_KL3w2fJn6GWwBxBHJpLbeUfq3-t-oPBxK0Yg</recordid><startdate>20200201</startdate><enddate>20200201</enddate><creator>Zheng, Jianyong</creator><creator>Wei, Wei</creator><creator>Wang, Shengfan</creator><creator>Li, Xiaojun</creator><creator>Zhang, Yinjun</creator><creator>Wang, Zhao</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9821-1062</orcidid><orcidid>https://orcid.org/0000-0002-9199-0059</orcidid></search><sort><creationdate>20200201</creationdate><title>Immobilization of Lipozyme TL 100L for methyl esterification of soybean oil deodorizer distillate</title><author>Zheng, Jianyong ; Wei, Wei ; Wang, Shengfan ; Li, Xiaojun ; Zhang, Yinjun ; Wang, Zhao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-13cc94a966332434162b1f6a4e477c7d1a154f38f18ae88eb00b7d31e40494313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Agriculture</topic><topic>Bioinformatics</topic><topic>Biomaterials</topic><topic>Biotechnology</topic><topic>Cancer Research</topic><topic>Catalytic activity</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Crosslinking</topic><topic>Distillates</topic><topic>Enzymes</topic><topic>Esterification</topic><topic>half life</topic><topic>Immobilization</topic><topic>Industrial applications</topic><topic>Lipase</topic><topic>Original</topic><topic>Original Article</topic><topic>porous media</topic><topic>Scanning electron microscopy</topic><topic>Soybean oil</topic><topic>Soybeans</topic><topic>Stem Cells</topic><topic>Surface structure</topic><topic>triacylglycerol lipase</topic><topic>Vegetable oils</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zheng, Jianyong</creatorcontrib><creatorcontrib>Wei, Wei</creatorcontrib><creatorcontrib>Wang, Shengfan</creatorcontrib><creatorcontrib>Li, Xiaojun</creatorcontrib><creatorcontrib>Zhang, Yinjun</creatorcontrib><creatorcontrib>Wang, Zhao</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>3 Biotech</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zheng, Jianyong</au><au>Wei, Wei</au><au>Wang, Shengfan</au><au>Li, Xiaojun</au><au>Zhang, Yinjun</au><au>Wang, Zhao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immobilization of Lipozyme TL 100L for methyl esterification of soybean oil deodorizer distillate</atitle><jtitle>3 Biotech</jtitle><stitle>3 Biotech</stitle><addtitle>3 Biotech</addtitle><date>2020-02-01</date><risdate>2020</risdate><volume>10</volume><issue>2</issue><spage>51</spage><epage>51</epage><pages>51-51</pages><artnum>51</artnum><issn>2190-572X</issn><eissn>2190-5738</eissn><abstract>An immobilization method for binding cross-linked enzyme aggregates of Lipozyme TL 100L on macroporous resin NKA (CLEA-TLL@NKA) was developed in this study. The esterification activity of CLEA-TLL@NKA reached 6.4 U/mg. The surface structure of immobilized lipase was characterized by scanning electron microscopy. Methyl esterification reaction of soybean oil deodorizer distillate (SODD) was catalyzed by CLEA-TLL@NKA, which the conversion rate reached 98% and its activity retained over 90% after 20 batches of reaction. Compared with the commercial enzyme Lipozyme TLIM, half-life (
t
1/2
) of CLEA-TLL@NKA increased by 25 times and the catalytic activity increased by approximate 10 times. Thus, CLEA-TLL@NKA had high catalytic activity, good operational stability, and potential industrial application in the field of oil processing.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>32002342</pmid><doi>10.1007/s13205-019-2028-6</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-9821-1062</orcidid><orcidid>https://orcid.org/0000-0002-9199-0059</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Agriculture Bioinformatics Biomaterials Biotechnology Cancer Research Catalytic activity Chemistry Chemistry and Materials Science Crosslinking Distillates Enzymes Esterification half life Immobilization Industrial applications Lipase Original Original Article porous media Scanning electron microscopy Soybean oil Soybeans Stem Cells Surface structure triacylglycerol lipase Vegetable oils |
| title | Immobilization of Lipozyme TL 100L for methyl esterification of soybean oil deodorizer distillate |
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