A Novel Thiophene-Based Fluorescent Chemosensor for the Detection of Zn2+ and CN−: Imaging Applications in Live Cells and Zebrafish
A novel fluorescent turn-on chemosensor DHADC ((E)-3-((4-(diethylamino)-2-hydroxybenzylidene)amino)-2,3-dihydrothiophene-2-carboxamide) has been developed and used to detect Zn2+ and CN−. Compound DHADC displayed a notable fluorescence increase with Zn2+. The limit of detection (2.55 ± 0.05 μM) for...
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| Veröffentlicht in: | Sensors (Basel, Switzerland) Switzerland), 2019-12, Vol.19 (24), p.5458 |
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| creator | Kim, Min Seon Yun, Dongju Chae, Ju Byeong So, Haeri Lee, Hyojin Kim, Ki-Tae Kim, Mingeun Lim, Mi Hee Kim, Cheal |
| description | A novel fluorescent turn-on chemosensor DHADC ((E)-3-((4-(diethylamino)-2-hydroxybenzylidene)amino)-2,3-dihydrothiophene-2-carboxamide) has been developed and used to detect Zn2+ and CN−. Compound DHADC displayed a notable fluorescence increase with Zn2+. The limit of detection (2.55 ± 0.05 μM) for zinc ion was far below the standard (76 μM) of the WHO (World Health Organization). In particular, compound DHADC could be applied to determine Zn2+ in real samples, and to image Zn2+ in both HeLa cells and zebrafish. Additionally, DHADC could detect CN− through a fluorescence enhancement with little inhibition with the existence of other types of anions. The detection processes of compound DHADC for Zn2+ and CN− were demonstrated with various analytical methods like Job plots, 1H NMR titrations, and ESI-Mass analyses. |
| doi_str_mv | 10.3390/s19245458 |
| format | Article |
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Compound DHADC displayed a notable fluorescence increase with Zn2+. The limit of detection (2.55 ± 0.05 μM) for zinc ion was far below the standard (76 μM) of the WHO (World Health Organization). In particular, compound DHADC could be applied to determine Zn2+ in real samples, and to image Zn2+ in both HeLa cells and zebrafish. Additionally, DHADC could detect CN− through a fluorescence enhancement with little inhibition with the existence of other types of anions. The detection processes of compound DHADC for Zn2+ and CN− were demonstrated with various analytical methods like Job plots, 1H NMR titrations, and ESI-Mass analyses.</description><identifier>ISSN: 1424-8220</identifier><identifier>EISSN: 1424-8220</identifier><identifier>DOI: 10.3390/s19245458</identifier><identifier>PMID: 31835755</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Chemical sensors ; Chemoreceptors ; Fluorescence ; Ions ; Solvents ; Zebrafish ; Zinc</subject><ispartof>Sensors (Basel, Switzerland), 2019-12, Vol.19 (24), p.5458</ispartof><rights>2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 by the authors. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c310t-1fc790f1f955cd987d99caf70f06d6ba79b9b9493e00d280008c96b3a24f28f63</citedby><cites>FETCH-LOGICAL-c310t-1fc790f1f955cd987d99caf70f06d6ba79b9b9493e00d280008c96b3a24f28f63</cites><orcidid>0000-0003-3377-4996</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6961029/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6961029/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,53770,53772</link.rule.ids></links><search><creatorcontrib>Kim, Min Seon</creatorcontrib><creatorcontrib>Yun, Dongju</creatorcontrib><creatorcontrib>Chae, Ju Byeong</creatorcontrib><creatorcontrib>So, Haeri</creatorcontrib><creatorcontrib>Lee, Hyojin</creatorcontrib><creatorcontrib>Kim, Ki-Tae</creatorcontrib><creatorcontrib>Kim, Mingeun</creatorcontrib><creatorcontrib>Lim, Mi Hee</creatorcontrib><creatorcontrib>Kim, Cheal</creatorcontrib><title>A Novel Thiophene-Based Fluorescent Chemosensor for the Detection of Zn2+ and CN−: Imaging Applications in Live Cells and Zebrafish</title><title>Sensors (Basel, Switzerland)</title><description>A novel fluorescent turn-on chemosensor DHADC ((E)-3-((4-(diethylamino)-2-hydroxybenzylidene)amino)-2,3-dihydrothiophene-2-carboxamide) has been developed and used to detect Zn2+ and CN−. Compound DHADC displayed a notable fluorescence increase with Zn2+. The limit of detection (2.55 ± 0.05 μM) for zinc ion was far below the standard (76 μM) of the WHO (World Health Organization). In particular, compound DHADC could be applied to determine Zn2+ in real samples, and to image Zn2+ in both HeLa cells and zebrafish. Additionally, DHADC could detect CN− through a fluorescence enhancement with little inhibition with the existence of other types of anions. The detection processes of compound DHADC for Zn2+ and CN− were demonstrated with various analytical methods like Job plots, 1H NMR titrations, and ESI-Mass analyses.</description><subject>Chemical sensors</subject><subject>Chemoreceptors</subject><subject>Fluorescence</subject><subject>Ions</subject><subject>Solvents</subject><subject>Zebrafish</subject><subject>Zinc</subject><issn>1424-8220</issn><issn>1424-8220</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpVkc1q3DAQx0VpyFdz6BsIeirFjT4s2-qhsHU-YdlekksuQpZHawWv5ErehTxAIOc8Yp6k3mwILcMwA_Pj_59hEPpMyXfOJTlNVLJc5KL6gA5pzvKsYox8_Kc_QEcp3RPCOOfVPjrgtOKiFOIQPc7wImygxzedC0MHHrJfOkGLL_p1iJAM-BHXHaxCAp9CxHbKsQN8BiOY0QWPg8V3nn3D2re4Xrw8Pf_A1yu9dH6JZ8PQO6O3WMLO47nbAK6h79MrfQdN1Nal7hPas7pPcPJWj9HtxflNfZXNf19e17N5ZjglY0atKSWx1EohTCurspXSaFsSS4q2aHQpmylyyYGQllWEkMrIouGa5ZZVtuDH6OdOd1g3K2i3x0XdqyG6lY4PKmin_p9416ll2KhCFpQwOQl8eROI4c8a0qjuwzr6aWfFBBeTNeN0or7uKBNDShHsuwMlavsx9f4x_hcY74hQ</recordid><startdate>20191211</startdate><enddate>20191211</enddate><creator>Kim, Min Seon</creator><creator>Yun, Dongju</creator><creator>Chae, Ju Byeong</creator><creator>So, Haeri</creator><creator>Lee, Hyojin</creator><creator>Kim, Ki-Tae</creator><creator>Kim, Mingeun</creator><creator>Lim, Mi Hee</creator><creator>Kim, Cheal</creator><general>MDPI AG</general><general>MDPI</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3377-4996</orcidid></search><sort><creationdate>20191211</creationdate><title>A Novel Thiophene-Based Fluorescent Chemosensor for the Detection of Zn2+ and CN−: Imaging Applications in Live Cells and Zebrafish</title><author>Kim, Min Seon ; Yun, Dongju ; Chae, Ju Byeong ; So, Haeri ; Lee, Hyojin ; Kim, Ki-Tae ; Kim, Mingeun ; Lim, Mi Hee ; Kim, Cheal</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c310t-1fc790f1f955cd987d99caf70f06d6ba79b9b9493e00d280008c96b3a24f28f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Chemical sensors</topic><topic>Chemoreceptors</topic><topic>Fluorescence</topic><topic>Ions</topic><topic>Solvents</topic><topic>Zebrafish</topic><topic>Zinc</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Min Seon</creatorcontrib><creatorcontrib>Yun, Dongju</creatorcontrib><creatorcontrib>Chae, Ju Byeong</creatorcontrib><creatorcontrib>So, Haeri</creatorcontrib><creatorcontrib>Lee, Hyojin</creatorcontrib><creatorcontrib>Kim, Ki-Tae</creatorcontrib><creatorcontrib>Kim, Mingeun</creatorcontrib><creatorcontrib>Lim, Mi Hee</creatorcontrib><creatorcontrib>Kim, Cheal</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Sensors (Basel, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Min Seon</au><au>Yun, Dongju</au><au>Chae, Ju Byeong</au><au>So, Haeri</au><au>Lee, Hyojin</au><au>Kim, Ki-Tae</au><au>Kim, Mingeun</au><au>Lim, Mi Hee</au><au>Kim, Cheal</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Novel Thiophene-Based Fluorescent Chemosensor for the Detection of Zn2+ and CN−: Imaging Applications in Live Cells and Zebrafish</atitle><jtitle>Sensors (Basel, Switzerland)</jtitle><date>2019-12-11</date><risdate>2019</risdate><volume>19</volume><issue>24</issue><spage>5458</spage><pages>5458-</pages><issn>1424-8220</issn><eissn>1424-8220</eissn><abstract>A novel fluorescent turn-on chemosensor DHADC ((E)-3-((4-(diethylamino)-2-hydroxybenzylidene)amino)-2,3-dihydrothiophene-2-carboxamide) has been developed and used to detect Zn2+ and CN−. 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| source | DOAJ Directory of Open Access Journals; MDPI - Multidisciplinary Digital Publishing Institute; PubMed Central; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library |
| subjects | Chemical sensors Chemoreceptors Fluorescence Ions Solvents Zebrafish Zinc |
| title | A Novel Thiophene-Based Fluorescent Chemosensor for the Detection of Zn2+ and CN−: Imaging Applications in Live Cells and Zebrafish |
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