Detection of the Plant Pathogen Pseudomonas Syringae pv. Lachrymans on Antibody-Modified Gold Electrodes by Electrochemical Impedance Spectroscopy
The present work describes an impedimetric immunosensor for pv. ( ) detection. This pathogen infects many crop species causing considerable yield losses, thus fast and cheap detection method is in high demand. In the assay, the gold disc electrode was modified with 4-aminothiophenol (4-ATP), glutara...
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| Veröffentlicht in: | Sensors (Basel, Switzerland) Switzerland), 2019-12, Vol.19 (24), p.5411 |
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| creator | Cebula, Zofia Żołędowska, Sabina Dziąbowska, Karolina Skwarecka, Marta Malinowska, Natalia Białobrzeska, Wioleta Czaczyk, Elżbieta Siuzdak, Katarzyna Sawczak, Mirosław Bogdanowicz, Robert Nidzworski, Dawid |
| description | The present work describes an impedimetric immunosensor for
pv.
(
) detection. This pathogen infects many crop species causing considerable yield losses, thus fast and cheap detection method is in high demand. In the assay, the gold disc electrode was modified with 4-aminothiophenol (4-ATP), glutaraldehyde (GA), and anti-Psl antibodies, and free-sites were blocked with bovine serum albumin (BSA). Sensor development was characterized by cyclic voltammetry (CV) and antigen detection by electrochemical impedance spectroscopy (EIS) measurements. Seven analyzed strains of
were verified as positive by the reference method (PCR) and this immunoassay, proving sensor specificity. Label-free electrochemical detection was in the linear range 1 × 10
-1.2 × 10
CFU/mL (colony-forming unit) with an R
coefficient of 0.992 and a detection limit (LOD) of 337 CFU/mL. The sensor did not interfere with negative probes like buffers and other bacteria. The assay was proven to be fast (10 min detection) and easy in preparation. The advantage was the simplicity and availability of the verified analyte (whole bacteria) as the method does not require sample pretreatment (e.g., DNA isolation). EIS biosensing technique was chosen as one of the simplest and most sensitive with the least destructive influence on the probes compared to other electrochemical methods. |
| doi_str_mv | 10.3390/s19245411 |
| format | Article |
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pv.
(
) detection. This pathogen infects many crop species causing considerable yield losses, thus fast and cheap detection method is in high demand. In the assay, the gold disc electrode was modified with 4-aminothiophenol (4-ATP), glutaraldehyde (GA), and anti-Psl antibodies, and free-sites were blocked with bovine serum albumin (BSA). Sensor development was characterized by cyclic voltammetry (CV) and antigen detection by electrochemical impedance spectroscopy (EIS) measurements. Seven analyzed strains of
were verified as positive by the reference method (PCR) and this immunoassay, proving sensor specificity. Label-free electrochemical detection was in the linear range 1 × 10
-1.2 × 10
CFU/mL (colony-forming unit) with an R
coefficient of 0.992 and a detection limit (LOD) of 337 CFU/mL. The sensor did not interfere with negative probes like buffers and other bacteria. The assay was proven to be fast (10 min detection) and easy in preparation. The advantage was the simplicity and availability of the verified analyte (whole bacteria) as the method does not require sample pretreatment (e.g., DNA isolation). EIS biosensing technique was chosen as one of the simplest and most sensitive with the least destructive influence on the probes compared to other electrochemical methods.</description><identifier>ISSN: 1424-8220</identifier><identifier>EISSN: 1424-8220</identifier><identifier>DOI: 10.3390/s19245411</identifier><identifier>PMID: 31835291</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Antibodies ; Antibodies - chemistry ; Aqueous solutions ; Bacteria ; Biosensing Techniques ; Crop diseases ; Dielectric Spectroscopy ; Electrochemical analysis ; Electrochemical impedance spectroscopy ; Electrodes ; Fruits ; Gold ; Gold - chemistry ; Immunoassay ; Immunosensors ; Methods ; Nanoparticles ; Pathogens ; Plant Diseases - genetics ; Plant Diseases - microbiology ; Potassium ; Pretreatment ; Pseudomonas ; Pseudomonas syringae - genetics ; Pseudomonas syringae - isolation & purification ; Pseudomonas syringae - pathogenicity ; Serum albumin ; Spectrum analysis ; Voltammetry</subject><ispartof>Sensors (Basel, Switzerland), 2019-12, Vol.19 (24), p.5411</ispartof><rights>2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 by the authors. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-4c783378c50527ad7f9128b6734c3717a6ec4a0d5367ce146164b577fdfe59a43</citedby><cites>FETCH-LOGICAL-c403t-4c783378c50527ad7f9128b6734c3717a6ec4a0d5367ce146164b577fdfe59a43</cites><orcidid>0000-0003-0533-0837 ; 0000-0001-6218-8342 ; 0000-0002-0226-3635 ; 0000-0002-1365-5351 ; 0000-0002-7543-2620</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6960962/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6960962/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31835291$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cebula, Zofia</creatorcontrib><creatorcontrib>Żołędowska, Sabina</creatorcontrib><creatorcontrib>Dziąbowska, Karolina</creatorcontrib><creatorcontrib>Skwarecka, Marta</creatorcontrib><creatorcontrib>Malinowska, Natalia</creatorcontrib><creatorcontrib>Białobrzeska, Wioleta</creatorcontrib><creatorcontrib>Czaczyk, Elżbieta</creatorcontrib><creatorcontrib>Siuzdak, Katarzyna</creatorcontrib><creatorcontrib>Sawczak, Mirosław</creatorcontrib><creatorcontrib>Bogdanowicz, Robert</creatorcontrib><creatorcontrib>Nidzworski, Dawid</creatorcontrib><title>Detection of the Plant Pathogen Pseudomonas Syringae pv. Lachrymans on Antibody-Modified Gold Electrodes by Electrochemical Impedance Spectroscopy</title><title>Sensors (Basel, Switzerland)</title><addtitle>Sensors (Basel)</addtitle><description>The present work describes an impedimetric immunosensor for
pv.
(
) detection. This pathogen infects many crop species causing considerable yield losses, thus fast and cheap detection method is in high demand. In the assay, the gold disc electrode was modified with 4-aminothiophenol (4-ATP), glutaraldehyde (GA), and anti-Psl antibodies, and free-sites were blocked with bovine serum albumin (BSA). Sensor development was characterized by cyclic voltammetry (CV) and antigen detection by electrochemical impedance spectroscopy (EIS) measurements. Seven analyzed strains of
were verified as positive by the reference method (PCR) and this immunoassay, proving sensor specificity. Label-free electrochemical detection was in the linear range 1 × 10
-1.2 × 10
CFU/mL (colony-forming unit) with an R
coefficient of 0.992 and a detection limit (LOD) of 337 CFU/mL. The sensor did not interfere with negative probes like buffers and other bacteria. The assay was proven to be fast (10 min detection) and easy in preparation. The advantage was the simplicity and availability of the verified analyte (whole bacteria) as the method does not require sample pretreatment (e.g., DNA isolation). EIS biosensing technique was chosen as one of the simplest and most sensitive with the least destructive influence on the probes compared to other electrochemical methods.</description><subject>Antibodies</subject><subject>Antibodies - chemistry</subject><subject>Aqueous solutions</subject><subject>Bacteria</subject><subject>Biosensing Techniques</subject><subject>Crop diseases</subject><subject>Dielectric Spectroscopy</subject><subject>Electrochemical analysis</subject><subject>Electrochemical impedance spectroscopy</subject><subject>Electrodes</subject><subject>Fruits</subject><subject>Gold</subject><subject>Gold - chemistry</subject><subject>Immunoassay</subject><subject>Immunosensors</subject><subject>Methods</subject><subject>Nanoparticles</subject><subject>Pathogens</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - microbiology</subject><subject>Potassium</subject><subject>Pretreatment</subject><subject>Pseudomonas</subject><subject>Pseudomonas syringae - genetics</subject><subject>Pseudomonas syringae - isolation & purification</subject><subject>Pseudomonas syringae - pathogenicity</subject><subject>Serum albumin</subject><subject>Spectrum analysis</subject><subject>Voltammetry</subject><issn>1424-8220</issn><issn>1424-8220</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkc1u1TAQhS1ERUthwQsgS2xgkeK_xMkGqSqlVLqIKxXWlmNPblwldrCTSnmNPnENba9KVx5rvjmaOQehd5SccN6Qz4k2TJSC0hfoiAomipox8vJJfYhep3RNCOOc16_QIac1L1lDj9DtV5jBzC54HDo894C3g_Yz3uq5DzvweJtgsWEMXid8tUbndxrwdHOCN9r0cR21TzgPn_rZtcGuxY9gXefA4oswWHw-ZPEYLCTcro8_08PojB7w5TiB1d4Avpr-dZIJ0_oGHXR6SPD24T1Gv7-d_zr7Xmx-XlyenW4KIwifC2FkzbmsTUlKJrWVXUNZ3VaSC8MllboCIzSxJa-kASoqWom2lLKzHZSNFvwYfbnXnZZ2BGvAz1EPaopu1HFVQTv1f8e7Xu3CjaqaijQVywIfHwRi-LNAmtXokoEhGwhhSSq7LQmhtZAZ_fAMvQ5L9Pk8xUpeioZWjGTq0z1lshUpQrdfhhL1N2m1Tzqz759uvycfo-V37kClUQ</recordid><startdate>20191209</startdate><enddate>20191209</enddate><creator>Cebula, Zofia</creator><creator>Żołędowska, Sabina</creator><creator>Dziąbowska, Karolina</creator><creator>Skwarecka, Marta</creator><creator>Malinowska, Natalia</creator><creator>Białobrzeska, Wioleta</creator><creator>Czaczyk, Elżbieta</creator><creator>Siuzdak, Katarzyna</creator><creator>Sawczak, Mirosław</creator><creator>Bogdanowicz, Robert</creator><creator>Nidzworski, Dawid</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-0533-0837</orcidid><orcidid>https://orcid.org/0000-0001-6218-8342</orcidid><orcidid>https://orcid.org/0000-0002-0226-3635</orcidid><orcidid>https://orcid.org/0000-0002-1365-5351</orcidid><orcidid>https://orcid.org/0000-0002-7543-2620</orcidid></search><sort><creationdate>20191209</creationdate><title>Detection of the Plant Pathogen Pseudomonas Syringae pv. Lachrymans on Antibody-Modified Gold Electrodes by Electrochemical Impedance Spectroscopy</title><author>Cebula, Zofia ; Żołędowska, Sabina ; Dziąbowska, Karolina ; Skwarecka, Marta ; Malinowska, Natalia ; Białobrzeska, Wioleta ; Czaczyk, Elżbieta ; Siuzdak, Katarzyna ; Sawczak, Mirosław ; Bogdanowicz, Robert ; Nidzworski, Dawid</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-4c783378c50527ad7f9128b6734c3717a6ec4a0d5367ce146164b577fdfe59a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antibodies</topic><topic>Antibodies - chemistry</topic><topic>Aqueous solutions</topic><topic>Bacteria</topic><topic>Biosensing Techniques</topic><topic>Crop diseases</topic><topic>Dielectric Spectroscopy</topic><topic>Electrochemical analysis</topic><topic>Electrochemical impedance spectroscopy</topic><topic>Electrodes</topic><topic>Fruits</topic><topic>Gold</topic><topic>Gold - chemistry</topic><topic>Immunoassay</topic><topic>Immunosensors</topic><topic>Methods</topic><topic>Nanoparticles</topic><topic>Pathogens</topic><topic>Plant Diseases - genetics</topic><topic>Plant Diseases - microbiology</topic><topic>Potassium</topic><topic>Pretreatment</topic><topic>Pseudomonas</topic><topic>Pseudomonas syringae - genetics</topic><topic>Pseudomonas syringae - isolation & purification</topic><topic>Pseudomonas syringae - pathogenicity</topic><topic>Serum albumin</topic><topic>Spectrum analysis</topic><topic>Voltammetry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cebula, Zofia</creatorcontrib><creatorcontrib>Żołędowska, Sabina</creatorcontrib><creatorcontrib>Dziąbowska, Karolina</creatorcontrib><creatorcontrib>Skwarecka, Marta</creatorcontrib><creatorcontrib>Malinowska, Natalia</creatorcontrib><creatorcontrib>Białobrzeska, Wioleta</creatorcontrib><creatorcontrib>Czaczyk, Elżbieta</creatorcontrib><creatorcontrib>Siuzdak, Katarzyna</creatorcontrib><creatorcontrib>Sawczak, Mirosław</creatorcontrib><creatorcontrib>Bogdanowicz, Robert</creatorcontrib><creatorcontrib>Nidzworski, Dawid</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Sensors (Basel, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cebula, Zofia</au><au>Żołędowska, Sabina</au><au>Dziąbowska, Karolina</au><au>Skwarecka, Marta</au><au>Malinowska, Natalia</au><au>Białobrzeska, Wioleta</au><au>Czaczyk, Elżbieta</au><au>Siuzdak, Katarzyna</au><au>Sawczak, Mirosław</au><au>Bogdanowicz, Robert</au><au>Nidzworski, Dawid</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of the Plant Pathogen Pseudomonas Syringae pv. Lachrymans on Antibody-Modified Gold Electrodes by Electrochemical Impedance Spectroscopy</atitle><jtitle>Sensors (Basel, Switzerland)</jtitle><addtitle>Sensors (Basel)</addtitle><date>2019-12-09</date><risdate>2019</risdate><volume>19</volume><issue>24</issue><spage>5411</spage><pages>5411-</pages><issn>1424-8220</issn><eissn>1424-8220</eissn><abstract>The present work describes an impedimetric immunosensor for
pv.
(
) detection. This pathogen infects many crop species causing considerable yield losses, thus fast and cheap detection method is in high demand. In the assay, the gold disc electrode was modified with 4-aminothiophenol (4-ATP), glutaraldehyde (GA), and anti-Psl antibodies, and free-sites were blocked with bovine serum albumin (BSA). Sensor development was characterized by cyclic voltammetry (CV) and antigen detection by electrochemical impedance spectroscopy (EIS) measurements. Seven analyzed strains of
were verified as positive by the reference method (PCR) and this immunoassay, proving sensor specificity. Label-free electrochemical detection was in the linear range 1 × 10
-1.2 × 10
CFU/mL (colony-forming unit) with an R
coefficient of 0.992 and a detection limit (LOD) of 337 CFU/mL. The sensor did not interfere with negative probes like buffers and other bacteria. The assay was proven to be fast (10 min detection) and easy in preparation. The advantage was the simplicity and availability of the verified analyte (whole bacteria) as the method does not require sample pretreatment (e.g., DNA isolation). EIS biosensing technique was chosen as one of the simplest and most sensitive with the least destructive influence on the probes compared to other electrochemical methods.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>31835291</pmid><doi>10.3390/s19245411</doi><orcidid>https://orcid.org/0000-0003-0533-0837</orcidid><orcidid>https://orcid.org/0000-0001-6218-8342</orcidid><orcidid>https://orcid.org/0000-0002-0226-3635</orcidid><orcidid>https://orcid.org/0000-0002-1365-5351</orcidid><orcidid>https://orcid.org/0000-0002-7543-2620</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Antibodies Antibodies - chemistry Aqueous solutions Bacteria Biosensing Techniques Crop diseases Dielectric Spectroscopy Electrochemical analysis Electrochemical impedance spectroscopy Electrodes Fruits Gold Gold - chemistry Immunoassay Immunosensors Methods Nanoparticles Pathogens Plant Diseases - genetics Plant Diseases - microbiology Potassium Pretreatment Pseudomonas Pseudomonas syringae - genetics Pseudomonas syringae - isolation & purification Pseudomonas syringae - pathogenicity Serum albumin Spectrum analysis Voltammetry |
| title | Detection of the Plant Pathogen Pseudomonas Syringae pv. Lachrymans on Antibody-Modified Gold Electrodes by Electrochemical Impedance Spectroscopy |
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