Analysis of allosteric communication in a multienzyme complex by ancestral sequence reconstruction

Tryptophan synthase (TS) is a heterotetrameric αββα complex. It is characterized by the channeling of the reaction intermediate indole and the mutual activation of the α-subunit TrpA and the β-subunit TrpB via a complex allosteric network. We have analyzed this allosteric network by means of ancestr...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2020-01, Vol.117 (1), p.346-354
Hauptverfasser:	Schupfner, Michael, Straub, Kristina, Busch, Florian, Merkl, Rainer, Sterner, Reinhard
Format:	Artikel
Sprache:	eng
Schlagworte:	Activation Allosteric properties Allosteric Regulation - genetics Amino Acid Sequence Amino acids Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Biological evolution Biological Sciences Channeling Computational Biology Computer simulation Extinction, Biological Indoles Intermediates Iterative methods Molecular dynamics Molecular Dynamics Simulation Multienzyme complexes Mutagenesis, Site-Directed Oceanospirillaceae - genetics Oceanospirillaceae - metabolism Phylogeny Protein Subunits - chemistry Protein Subunits - genetics Protein Subunits - metabolism Reconstruction Residues Sequence Alignment Site-directed mutagenesis Structural Homology, Protein Tryptophan Tryptophan - biosynthesis Tryptophan synthase Tryptophan Synthase - chemistry Tryptophan Synthase - genetics Tryptophan Synthase - metabolism
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