Deoxynivalenol Induces Inflammatory Injury in IPEC-J2 Cells via NF-κB Signaling Pathway
The aim of this study was to investigate the effects of deoxynivalenol (DON) exposure on the inflammatory injury nuclear factor kappa-B (NF-κB) pathway in intestinal epithelial cells (IPEC-J2 cells) of pig. The different concentrations of DON (0, 125, 250, 500, 1000, 2000 ng/mL) were added to the cu...
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| Veröffentlicht in: | Toxins 2019-12, Vol.11 (12), p.733 |
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| creator | Wang, Xichun Zhang, Yafei Zhao, Jie Cao, Li Zhu, Lei Huang, Yingying Chen, Xiaofang Rahman, Sajid Ur Feng, Shibin Li, Yu Wu, Jinjie |
| description | The aim of this study was to investigate the effects of deoxynivalenol (DON) exposure on the inflammatory injury nuclear factor kappa-B (NF-κB) pathway in intestinal epithelial cells (IPEC-J2 cells) of pig. The different concentrations of DON (0, 125, 250, 500, 1000, 2000 ng/mL) were added to the culture solution for treatment. The NF-κB pathway inhibitor pyrrolidine dithiocarbamate (PDTC) was used as a reference. The results showed that when the DON concentration increased, the cell density decreased and seemed damaged. With the increase of DON concentration in the culture medium, the action of diamine oxidase (DAO) in the culture supernatant also increased. The activities of IL-6, TNF-α, and NO in the cells were increased with the increasing DON concentration. The relative mRNA expression of
and
were increased in the cells. The mRNA relative expression of
,
and
were upregulated with the increasing of DON concentration, while the relative expression of
mRNA was downregulated. At the same time, the expression of NF-κB p65 protein increased gradually in the cytoplasm and nucleus with a higher concentration of DON. These results showed that DON could change the morphology of IPEC-J2 cells, destroy its submicroscopic structure, and enhance the permeability of cell membrane, as well as upregulate the transcription of some inflammatory factors and change the expression of NF-κB-related gene or protein in cells. |
| doi_str_mv | 10.3390/toxins11120733 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6950076</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2550280717</sourcerecordid><originalsourceid>FETCH-LOGICAL-c418t-c020d78c8142c54459e471e7ec32b5c9c04b5018738f287f5e8916726245c97d3</originalsourceid><addsrcrecordid>eNpdkd1KwzAcxYMoTuZuvZSCN95U89E06Y2g9WsiOlDBu5Bl6cxoE23aaV_Nh_CZjGzKZm5Owv-XQ04OAHsIHhGSwePGfRjrEUIYMkI2wE5QHKcpRZsr-x4YeD-DYRGCMsS2QY8gzjnO2A54Ptfuo7NmLkttXRkN7aRV2gctSllVsnF1Fw6zNoix0XB0kcc3OMp1WfpobmR0dxl_fZ5FD2ZqZWnsNBrJ5uVddrtgq5Cl14Ol9sHT5cVjfh3f3l8N89PbWCWIN7GCGE4YVxwlWNEkoZlOGNJMK4LHVGUKJmMKEWeEF5izgmqeoZThFCdhyiakD04Wvq_tuNITpW1Ty1K81qaSdSecNGJ9Ys2LmLq5SDMKIUuDweHSoHZvrfaNqIxXIZ-02rVe4PBrKSIkIQE9-IfOXFuH3IGiFGIOGWKBOlpQqnbe17r4ewyC4qc3sd5buLC_GuEP_22JfAOxc5OD</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2550280717</pqid></control><display><type>article</type><title>Deoxynivalenol Induces Inflammatory Injury in IPEC-J2 Cells via NF-κB Signaling Pathway</title><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central Open Access</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Wang, Xichun ; Zhang, Yafei ; Zhao, Jie ; Cao, Li ; Zhu, Lei ; Huang, Yingying ; Chen, Xiaofang ; Rahman, Sajid Ur ; Feng, Shibin ; Li, Yu ; Wu, Jinjie</creator><creatorcontrib>Wang, Xichun ; Zhang, Yafei ; Zhao, Jie ; Cao, Li ; Zhu, Lei ; Huang, Yingying ; Chen, Xiaofang ; Rahman, Sajid Ur ; Feng, Shibin ; Li, Yu ; Wu, Jinjie</creatorcontrib><description>The aim of this study was to investigate the effects of deoxynivalenol (DON) exposure on the inflammatory injury nuclear factor kappa-B (NF-κB) pathway in intestinal epithelial cells (IPEC-J2 cells) of pig. The different concentrations of DON (0, 125, 250, 500, 1000, 2000 ng/mL) were added to the culture solution for treatment. The NF-κB pathway inhibitor pyrrolidine dithiocarbamate (PDTC) was used as a reference. The results showed that when the DON concentration increased, the cell density decreased and seemed damaged. With the increase of DON concentration in the culture medium, the action of diamine oxidase (DAO) in the culture supernatant also increased. The activities of IL-6, TNF-α, and NO in the cells were increased with the increasing DON concentration. The relative mRNA expression of
and
were increased in the cells. The mRNA relative expression of
,
and
were upregulated with the increasing of DON concentration, while the relative expression of
mRNA was downregulated. At the same time, the expression of NF-κB p65 protein increased gradually in the cytoplasm and nucleus with a higher concentration of DON. These results showed that DON could change the morphology of IPEC-J2 cells, destroy its submicroscopic structure, and enhance the permeability of cell membrane, as well as upregulate the transcription of some inflammatory factors and change the expression of NF-κB-related gene or protein in cells.</description><identifier>ISSN: 2072-6651</identifier><identifier>EISSN: 2072-6651</identifier><identifier>DOI: 10.3390/toxins11120733</identifier><identifier>PMID: 31888297</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Cell culture ; Cell density ; Cell membranes ; Cytology ; Cytoplasm ; Deoxynivalenol ; Diamines ; Epithelial cells ; Epithelium ; Feeds ; Gene expression ; Genes ; Hogs ; IL-1β ; Inflammation ; Interleukin 6 ; Membrane permeability ; Morphology ; NF-κB protein ; Proteins ; Pyrrolidine ; Pyrrolidine dithiocarbamate ; Signal transduction ; Synaptotagmin ; Transcription factors ; Tumor necrosis factor-α</subject><ispartof>Toxins, 2019-12, Vol.11 (12), p.733</ispartof><rights>2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 by the authors. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-c020d78c8142c54459e471e7ec32b5c9c04b5018738f287f5e8916726245c97d3</citedby><cites>FETCH-LOGICAL-c418t-c020d78c8142c54459e471e7ec32b5c9c04b5018738f287f5e8916726245c97d3</cites><orcidid>0000-0002-5969-5463 ; 0000-0002-9886-4952 ; 0000-0001-5096-3970</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6950076/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6950076/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31888297$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Xichun</creatorcontrib><creatorcontrib>Zhang, Yafei</creatorcontrib><creatorcontrib>Zhao, Jie</creatorcontrib><creatorcontrib>Cao, Li</creatorcontrib><creatorcontrib>Zhu, Lei</creatorcontrib><creatorcontrib>Huang, Yingying</creatorcontrib><creatorcontrib>Chen, Xiaofang</creatorcontrib><creatorcontrib>Rahman, Sajid Ur</creatorcontrib><creatorcontrib>Feng, Shibin</creatorcontrib><creatorcontrib>Li, Yu</creatorcontrib><creatorcontrib>Wu, Jinjie</creatorcontrib><title>Deoxynivalenol Induces Inflammatory Injury in IPEC-J2 Cells via NF-κB Signaling Pathway</title><title>Toxins</title><addtitle>Toxins (Basel)</addtitle><description>The aim of this study was to investigate the effects of deoxynivalenol (DON) exposure on the inflammatory injury nuclear factor kappa-B (NF-κB) pathway in intestinal epithelial cells (IPEC-J2 cells) of pig. The different concentrations of DON (0, 125, 250, 500, 1000, 2000 ng/mL) were added to the culture solution for treatment. The NF-κB pathway inhibitor pyrrolidine dithiocarbamate (PDTC) was used as a reference. The results showed that when the DON concentration increased, the cell density decreased and seemed damaged. With the increase of DON concentration in the culture medium, the action of diamine oxidase (DAO) in the culture supernatant also increased. The activities of IL-6, TNF-α, and NO in the cells were increased with the increasing DON concentration. The relative mRNA expression of
and
were increased in the cells. The mRNA relative expression of
,
and
were upregulated with the increasing of DON concentration, while the relative expression of
mRNA was downregulated. At the same time, the expression of NF-κB p65 protein increased gradually in the cytoplasm and nucleus with a higher concentration of DON. These results showed that DON could change the morphology of IPEC-J2 cells, destroy its submicroscopic structure, and enhance the permeability of cell membrane, as well as upregulate the transcription of some inflammatory factors and change the expression of NF-κB-related gene or protein in cells.</description><subject>Cell culture</subject><subject>Cell density</subject><subject>Cell membranes</subject><subject>Cytology</subject><subject>Cytoplasm</subject><subject>Deoxynivalenol</subject><subject>Diamines</subject><subject>Epithelial cells</subject><subject>Epithelium</subject><subject>Feeds</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Hogs</subject><subject>IL-1β</subject><subject>Inflammation</subject><subject>Interleukin 6</subject><subject>Membrane permeability</subject><subject>Morphology</subject><subject>NF-κB protein</subject><subject>Proteins</subject><subject>Pyrrolidine</subject><subject>Pyrrolidine dithiocarbamate</subject><subject>Signal 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Induces Inflammatory Injury in IPEC-J2 Cells via NF-κB Signaling Pathway</title><author>Wang, Xichun ; Zhang, Yafei ; Zhao, Jie ; Cao, Li ; Zhu, Lei ; Huang, Yingying ; Chen, Xiaofang ; Rahman, Sajid Ur ; Feng, Shibin ; Li, Yu ; Wu, Jinjie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-c020d78c8142c54459e471e7ec32b5c9c04b5018738f287f5e8916726245c97d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Cell culture</topic><topic>Cell density</topic><topic>Cell membranes</topic><topic>Cytology</topic><topic>Cytoplasm</topic><topic>Deoxynivalenol</topic><topic>Diamines</topic><topic>Epithelial cells</topic><topic>Epithelium</topic><topic>Feeds</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Hogs</topic><topic>IL-1β</topic><topic>Inflammation</topic><topic>Interleukin 6</topic><topic>Membrane permeability</topic><topic>Morphology</topic><topic>NF-κB protein</topic><topic>Proteins</topic><topic>Pyrrolidine</topic><topic>Pyrrolidine dithiocarbamate</topic><topic>Signal transduction</topic><topic>Synaptotagmin</topic><topic>Transcription factors</topic><topic>Tumor necrosis factor-α</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Xichun</creatorcontrib><creatorcontrib>Zhang, Yafei</creatorcontrib><creatorcontrib>Zhao, Jie</creatorcontrib><creatorcontrib>Cao, Li</creatorcontrib><creatorcontrib>Zhu, Lei</creatorcontrib><creatorcontrib>Huang, Yingying</creatorcontrib><creatorcontrib>Chen, Xiaofang</creatorcontrib><creatorcontrib>Rahman, Sajid Ur</creatorcontrib><creatorcontrib>Feng, Shibin</creatorcontrib><creatorcontrib>Li, Yu</creatorcontrib><creatorcontrib>Wu, Jinjie</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology 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Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Toxins</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Xichun</au><au>Zhang, Yafei</au><au>Zhao, Jie</au><au>Cao, Li</au><au>Zhu, Lei</au><au>Huang, Yingying</au><au>Chen, Xiaofang</au><au>Rahman, Sajid Ur</au><au>Feng, Shibin</au><au>Li, Yu</au><au>Wu, Jinjie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Deoxynivalenol Induces Inflammatory Injury in IPEC-J2 Cells via NF-κB Signaling Pathway</atitle><jtitle>Toxins</jtitle><addtitle>Toxins (Basel)</addtitle><date>2019-12-16</date><risdate>2019</risdate><volume>11</volume><issue>12</issue><spage>733</spage><pages>733-</pages><issn>2072-6651</issn><eissn>2072-6651</eissn><abstract>The aim of this study was to investigate the effects of deoxynivalenol (DON) exposure on the inflammatory injury nuclear factor kappa-B (NF-κB) pathway in intestinal epithelial cells (IPEC-J2 cells) of pig. The different concentrations of DON (0, 125, 250, 500, 1000, 2000 ng/mL) were added to the culture solution for treatment. The NF-κB pathway inhibitor pyrrolidine dithiocarbamate (PDTC) was used as a reference. The results showed that when the DON concentration increased, the cell density decreased and seemed damaged. With the increase of DON concentration in the culture medium, the action of diamine oxidase (DAO) in the culture supernatant also increased. The activities of IL-6, TNF-α, and NO in the cells were increased with the increasing DON concentration. The relative mRNA expression of
and
were increased in the cells. The mRNA relative expression of
,
and
were upregulated with the increasing of DON concentration, while the relative expression of
mRNA was downregulated. At the same time, the expression of NF-κB p65 protein increased gradually in the cytoplasm and nucleus with a higher concentration of DON. These results showed that DON could change the morphology of IPEC-J2 cells, destroy its submicroscopic structure, and enhance the permeability of cell membrane, as well as upregulate the transcription of some inflammatory factors and change the expression of NF-κB-related gene or protein in cells.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>31888297</pmid><doi>10.3390/toxins11120733</doi><orcidid>https://orcid.org/0000-0002-5969-5463</orcidid><orcidid>https://orcid.org/0000-0002-9886-4952</orcidid><orcidid>https://orcid.org/0000-0001-5096-3970</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Cell culture Cell density Cell membranes Cytology Cytoplasm Deoxynivalenol Diamines Epithelial cells Epithelium Feeds Gene expression Genes Hogs IL-1β Inflammation Interleukin 6 Membrane permeability Morphology NF-κB protein Proteins Pyrrolidine Pyrrolidine dithiocarbamate Signal transduction Synaptotagmin Transcription factors Tumor necrosis factor-α |
| title | Deoxynivalenol Induces Inflammatory Injury in IPEC-J2 Cells via NF-κB Signaling Pathway |
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