Citrullinome of Porphyromonas gingivalis Outer Membrane Vesicles: Confident Identification of Citrullinated Peptides

The citrullinome of P. gingivalis outer membrane vesicles (OMV) has been explored by a novel two-dimensional separation system combined with high resolution mass spectrometry and in-house build software. Analysis of OMVs from wild-type and two PPAD mutants resulted in confident discrimination based...

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Veröffentlicht in:	Molecular & cellular proteomics 2020-01, Vol.19 (1), p.167-180
Hauptverfasser:	Larsen, Daniel Nyberg, Mikkelsen, Christian Engelbrecht, Kierkegaard, Mads, Bereta, Grzegorz P., Nowakowska, Zuzanna, Kaczmarek, Jakub Z., Potempa, Jan, Højrup, Peter
Format:	Artikel
Sprache:	eng
Schlagworte:	Alanine - metabolism Arthritis, Rheumatoid - microbiology bacteria Bacterial Outer Membrane - metabolism Bacterial Proteins - metabolism bioinformatics software Catalytic Domain Chromatography Chromatography, Liquid Citrullination Extracellular Vesicles - metabolism Gene Knockout Techniques HFBA HPLC Humans Mass Spectrometry Membrane Proteins - metabolism omics orthogonal Peptides - metabolism Porphyromonas gingivalis - metabolism post-translational modifications protein identification protein modification Protein-Arginine Deiminases - genetics Protein-Arginine Deiminases - metabolism Proteomics - methods two-dimensional
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