Waste Autochthonous Tuscan Olive Leaves ( Olea europaea var. Olivastra seggianese ) as Antioxidant Source for Biomedicine
Olive leaf extract (OLE) can be obtained as biowaste and is extensively used a food supplement and an over-the-counter drug for its beneficial effects. New studies have investigated OLE concerning the role of oxidative stress in the pathogenesis of vascular disease. This in vitro study aims to evalu...
Gespeichert in:
| Veröffentlicht in: | International journal of molecular sciences 2019-11, Vol.20 (23), p.5918 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 23 |
| container_start_page | 5918 |
| container_title | International journal of molecular sciences |
| container_volume | 20 |
| creator | De la Ossa, Jose Gustavo Felice, Francesca Azimi, Bahareh Salsano, Jasmine Esposito Digiacomo, Maria Macchia, Marco Danti, Serena Di Stefano, Rossella |
| description | Olive leaf extract (OLE) can be obtained as biowaste and is extensively used a food supplement and an over-the-counter drug for its beneficial effects. New studies have investigated OLE concerning the role of oxidative stress in the pathogenesis of vascular disease. This in vitro study aims to evaluate if OLE extracted from the Tuscan
protects endothelial cells against oxidative stress generated by reactive oxygen species (ROS).
OLE total polyphenols (TPs) were characterized by the Folin-Ciocalteu method. Endothelial cells were grown in conventional cultures (i.e., two-dimensional, 2D) and on a biomaterial scaffold (i.e., three-dimensional, 3D) fabricated via electrospinning. Cell viability and ROS measurement after H
O
insults were performed.
OLE TP content was 23.29 mg GAE/g, and oleuropein was the principal compound. The dose-dependent viability curve highlighted the absence of significant cytotoxic effects at OLE concentrations below 250 µg/mL TPs. By using OLE preconditioning at 100 µg/mL, cell viability decrease was observed, being in 3D lower than in the 2D model. OLE was protective against ROS in both models.
OLE represents a high-value antioxidant source obtained by biowaste that is interesting for biomedical products. Using a 3D scaffold could be the best predictive model to mimic the physiological conditions of vascular tissue reaction. |
| doi_str_mv | 10.3390/ijms20235918 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6928929</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2548650851</sourcerecordid><originalsourceid>FETCH-LOGICAL-c412t-9f4ce1a64b6b5abe9092566124899119f44ab27714e205e82c7fe26f34ee8acd3</originalsourceid><addsrcrecordid>eNpdkc1PGzEQxS1UBBR644ws9QJSQ22vvWtfkFLUQqVIHArq0Zp1ZhNHGzu1d6Py3-OWD6U9eaz5zfM8P0JOObusKsM--9U6CyYqZbjeI0dcCjFhrG7e7dSH5H3OK1YoocwBOax406gyfUQef0IekE7HIbrlsIwhjpnej9lBoHe93yKdIWwx0_NyRaA4priBUmwhXf4lynwCmnGx8BAwI72gkOk0DD7-9nMIA_0Rx-SQdjHRLz6uce6dD3hC9jvoM354OY_Jw7ev99e3k9ndzffr6WziJBfDxHTSIYdatnWroEXDjFB1zYXUxnBe2hJa0TRcomAKtXBNh6LuKomowc2rY3L1rLsZ2_K2w1D27e0m-TWkRxvB2387wS_tIm5tbYQ2whSB8xeBFH-NmAe79tlh3xe75besqLiRRmvGCvrxP3RVvIdizwolda2YVrxQn54pl2LOCbu3ZTizfzK1u5kW_GzXwBv8GmL1BL4vnkA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2548650851</pqid></control><display><type>article</type><title>Waste Autochthonous Tuscan Olive Leaves ( Olea europaea var. Olivastra seggianese ) as Antioxidant Source for Biomedicine</title><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>De la Ossa, Jose Gustavo ; Felice, Francesca ; Azimi, Bahareh ; Salsano, Jasmine Esposito ; Digiacomo, Maria ; Macchia, Marco ; Danti, Serena ; Di Stefano, Rossella</creator><creatorcontrib>De la Ossa, Jose Gustavo ; Felice, Francesca ; Azimi, Bahareh ; Salsano, Jasmine Esposito ; Digiacomo, Maria ; Macchia, Marco ; Danti, Serena ; Di Stefano, Rossella</creatorcontrib><description>Olive leaf extract (OLE) can be obtained as biowaste and is extensively used a food supplement and an over-the-counter drug for its beneficial effects. New studies have investigated OLE concerning the role of oxidative stress in the pathogenesis of vascular disease. This in vitro study aims to evaluate if OLE extracted from the Tuscan
protects endothelial cells against oxidative stress generated by reactive oxygen species (ROS).
OLE total polyphenols (TPs) were characterized by the Folin-Ciocalteu method. Endothelial cells were grown in conventional cultures (i.e., two-dimensional, 2D) and on a biomaterial scaffold (i.e., three-dimensional, 3D) fabricated via electrospinning. Cell viability and ROS measurement after H
O
insults were performed.
OLE TP content was 23.29 mg GAE/g, and oleuropein was the principal compound. The dose-dependent viability curve highlighted the absence of significant cytotoxic effects at OLE concentrations below 250 µg/mL TPs. By using OLE preconditioning at 100 µg/mL, cell viability decrease was observed, being in 3D lower than in the 2D model. OLE was protective against ROS in both models.
OLE represents a high-value antioxidant source obtained by biowaste that is interesting for biomedical products. Using a 3D scaffold could be the best predictive model to mimic the physiological conditions of vascular tissue reaction.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms20235918</identifier><identifier>PMID: 31775339</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Antioxidants ; Antioxidants - pharmacology ; Biomaterials ; Biomedical materials ; Cell Survival ; Cell viability ; Cytotoxicity ; Drug dosages ; Endothelial cells ; Endothelium, Vascular - cytology ; Endothelium, Vascular - drug effects ; Fluorides ; Humans ; Hydrogen peroxide ; Metabolism ; Microscopy ; Morphology ; Olea - chemistry ; Olea europaea ; Oleuropein ; Oxidative stress ; Oxidative Stress - drug effects ; Plant extracts ; Plant Extracts - pharmacology ; Plant Leaves - chemistry ; Polyphenols ; Preconditioning ; Prediction models ; Protective Agents - pharmacology ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Scaffolds ; Tissue engineering ; Two dimensional models ; Vascular diseases ; Vascular tissue</subject><ispartof>International journal of molecular sciences, 2019-11, Vol.20 (23), p.5918</ispartof><rights>2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 by the authors. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-9f4ce1a64b6b5abe9092566124899119f44ab27714e205e82c7fe26f34ee8acd3</citedby><cites>FETCH-LOGICAL-c412t-9f4ce1a64b6b5abe9092566124899119f44ab27714e205e82c7fe26f34ee8acd3</cites><orcidid>0000-0001-8968-2228 ; 0000-0003-0653-6642 ; 0000-0002-8155-8537</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928929/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928929/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31775339$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>De la Ossa, Jose Gustavo</creatorcontrib><creatorcontrib>Felice, Francesca</creatorcontrib><creatorcontrib>Azimi, Bahareh</creatorcontrib><creatorcontrib>Salsano, Jasmine Esposito</creatorcontrib><creatorcontrib>Digiacomo, Maria</creatorcontrib><creatorcontrib>Macchia, Marco</creatorcontrib><creatorcontrib>Danti, Serena</creatorcontrib><creatorcontrib>Di Stefano, Rossella</creatorcontrib><title>Waste Autochthonous Tuscan Olive Leaves ( Olea europaea var. Olivastra seggianese ) as Antioxidant Source for Biomedicine</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Olive leaf extract (OLE) can be obtained as biowaste and is extensively used a food supplement and an over-the-counter drug for its beneficial effects. New studies have investigated OLE concerning the role of oxidative stress in the pathogenesis of vascular disease. This in vitro study aims to evaluate if OLE extracted from the Tuscan
protects endothelial cells against oxidative stress generated by reactive oxygen species (ROS).
OLE total polyphenols (TPs) were characterized by the Folin-Ciocalteu method. Endothelial cells were grown in conventional cultures (i.e., two-dimensional, 2D) and on a biomaterial scaffold (i.e., three-dimensional, 3D) fabricated via electrospinning. Cell viability and ROS measurement after H
O
insults were performed.
OLE TP content was 23.29 mg GAE/g, and oleuropein was the principal compound. The dose-dependent viability curve highlighted the absence of significant cytotoxic effects at OLE concentrations below 250 µg/mL TPs. By using OLE preconditioning at 100 µg/mL, cell viability decrease was observed, being in 3D lower than in the 2D model. OLE was protective against ROS in both models.
OLE represents a high-value antioxidant source obtained by biowaste that is interesting for biomedical products. Using a 3D scaffold could be the best predictive model to mimic the physiological conditions of vascular tissue reaction.</description><subject>Antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>Biomaterials</subject><subject>Biomedical materials</subject><subject>Cell Survival</subject><subject>Cell viability</subject><subject>Cytotoxicity</subject><subject>Drug dosages</subject><subject>Endothelial cells</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Fluorides</subject><subject>Humans</subject><subject>Hydrogen peroxide</subject><subject>Metabolism</subject><subject>Microscopy</subject><subject>Morphology</subject><subject>Olea - chemistry</subject><subject>Olea europaea</subject><subject>Oleuropein</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Plant extracts</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Leaves - chemistry</subject><subject>Polyphenols</subject><subject>Preconditioning</subject><subject>Prediction models</subject><subject>Protective Agents - pharmacology</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Scaffolds</subject><subject>Tissue engineering</subject><subject>Two dimensional models</subject><subject>Vascular diseases</subject><subject>Vascular tissue</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkc1PGzEQxS1UBBR644ws9QJSQ22vvWtfkFLUQqVIHArq0Zp1ZhNHGzu1d6Py3-OWD6U9eaz5zfM8P0JOObusKsM--9U6CyYqZbjeI0dcCjFhrG7e7dSH5H3OK1YoocwBOax406gyfUQef0IekE7HIbrlsIwhjpnej9lBoHe93yKdIWwx0_NyRaA4priBUmwhXf4lynwCmnGx8BAwI72gkOk0DD7-9nMIA_0Rx-SQdjHRLz6uce6dD3hC9jvoM354OY_Jw7ev99e3k9ndzffr6WziJBfDxHTSIYdatnWroEXDjFB1zYXUxnBe2hJa0TRcomAKtXBNh6LuKomowc2rY3L1rLsZ2_K2w1D27e0m-TWkRxvB2387wS_tIm5tbYQ2whSB8xeBFH-NmAe79tlh3xe75besqLiRRmvGCvrxP3RVvIdizwolda2YVrxQn54pl2LOCbu3ZTizfzK1u5kW_GzXwBv8GmL1BL4vnkA</recordid><startdate>20191125</startdate><enddate>20191125</enddate><creator>De la Ossa, Jose Gustavo</creator><creator>Felice, Francesca</creator><creator>Azimi, Bahareh</creator><creator>Salsano, Jasmine Esposito</creator><creator>Digiacomo, Maria</creator><creator>Macchia, Marco</creator><creator>Danti, Serena</creator><creator>Di Stefano, Rossella</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-8968-2228</orcidid><orcidid>https://orcid.org/0000-0003-0653-6642</orcidid><orcidid>https://orcid.org/0000-0002-8155-8537</orcidid></search><sort><creationdate>20191125</creationdate><title>Waste Autochthonous Tuscan Olive Leaves ( Olea europaea var. Olivastra seggianese ) as Antioxidant Source for Biomedicine</title><author>De la Ossa, Jose Gustavo ; Felice, Francesca ; Azimi, Bahareh ; Salsano, Jasmine Esposito ; Digiacomo, Maria ; Macchia, Marco ; Danti, Serena ; Di Stefano, Rossella</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-9f4ce1a64b6b5abe9092566124899119f44ab27714e205e82c7fe26f34ee8acd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antioxidants</topic><topic>Antioxidants - pharmacology</topic><topic>Biomaterials</topic><topic>Biomedical materials</topic><topic>Cell Survival</topic><topic>Cell viability</topic><topic>Cytotoxicity</topic><topic>Drug dosages</topic><topic>Endothelial cells</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Fluorides</topic><topic>Humans</topic><topic>Hydrogen peroxide</topic><topic>Metabolism</topic><topic>Microscopy</topic><topic>Morphology</topic><topic>Olea - chemistry</topic><topic>Olea europaea</topic><topic>Oleuropein</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Plant extracts</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Leaves - chemistry</topic><topic>Polyphenols</topic><topic>Preconditioning</topic><topic>Prediction models</topic><topic>Protective Agents - pharmacology</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Scaffolds</topic><topic>Tissue engineering</topic><topic>Two dimensional models</topic><topic>Vascular diseases</topic><topic>Vascular tissue</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De la Ossa, Jose Gustavo</creatorcontrib><creatorcontrib>Felice, Francesca</creatorcontrib><creatorcontrib>Azimi, Bahareh</creatorcontrib><creatorcontrib>Salsano, Jasmine Esposito</creatorcontrib><creatorcontrib>Digiacomo, Maria</creatorcontrib><creatorcontrib>Macchia, Marco</creatorcontrib><creatorcontrib>Danti, Serena</creatorcontrib><creatorcontrib>Di Stefano, Rossella</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De la Ossa, Jose Gustavo</au><au>Felice, Francesca</au><au>Azimi, Bahareh</au><au>Salsano, Jasmine Esposito</au><au>Digiacomo, Maria</au><au>Macchia, Marco</au><au>Danti, Serena</au><au>Di Stefano, Rossella</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Waste Autochthonous Tuscan Olive Leaves ( Olea europaea var. Olivastra seggianese ) as Antioxidant Source for Biomedicine</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2019-11-25</date><risdate>2019</risdate><volume>20</volume><issue>23</issue><spage>5918</spage><pages>5918-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Olive leaf extract (OLE) can be obtained as biowaste and is extensively used a food supplement and an over-the-counter drug for its beneficial effects. New studies have investigated OLE concerning the role of oxidative stress in the pathogenesis of vascular disease. This in vitro study aims to evaluate if OLE extracted from the Tuscan
protects endothelial cells against oxidative stress generated by reactive oxygen species (ROS).
OLE total polyphenols (TPs) were characterized by the Folin-Ciocalteu method. Endothelial cells were grown in conventional cultures (i.e., two-dimensional, 2D) and on a biomaterial scaffold (i.e., three-dimensional, 3D) fabricated via electrospinning. Cell viability and ROS measurement after H
O
insults were performed.
OLE TP content was 23.29 mg GAE/g, and oleuropein was the principal compound. The dose-dependent viability curve highlighted the absence of significant cytotoxic effects at OLE concentrations below 250 µg/mL TPs. By using OLE preconditioning at 100 µg/mL, cell viability decrease was observed, being in 3D lower than in the 2D model. OLE was protective against ROS in both models.
OLE represents a high-value antioxidant source obtained by biowaste that is interesting for biomedical products. Using a 3D scaffold could be the best predictive model to mimic the physiological conditions of vascular tissue reaction.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>31775339</pmid><doi>10.3390/ijms20235918</doi><orcidid>https://orcid.org/0000-0001-8968-2228</orcidid><orcidid>https://orcid.org/0000-0003-0653-6642</orcidid><orcidid>https://orcid.org/0000-0002-8155-8537</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1422-0067 |
| ispartof | International journal of molecular sciences, 2019-11, Vol.20 (23), p.5918 |
| issn | 1422-0067 1661-6596 1422-0067 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6928929 |
| source | MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Antioxidants Antioxidants - pharmacology Biomaterials Biomedical materials Cell Survival Cell viability Cytotoxicity Drug dosages Endothelial cells Endothelium, Vascular - cytology Endothelium, Vascular - drug effects Fluorides Humans Hydrogen peroxide Metabolism Microscopy Morphology Olea - chemistry Olea europaea Oleuropein Oxidative stress Oxidative Stress - drug effects Plant extracts Plant Extracts - pharmacology Plant Leaves - chemistry Polyphenols Preconditioning Prediction models Protective Agents - pharmacology Reactive oxygen species Reactive Oxygen Species - metabolism Scaffolds Tissue engineering Two dimensional models Vascular diseases Vascular tissue |
| title | Waste Autochthonous Tuscan Olive Leaves ( Olea europaea var. Olivastra seggianese ) as Antioxidant Source for Biomedicine |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T04%3A10%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Waste%20Autochthonous%20Tuscan%20Olive%20Leaves%20(%20Olea%20europaea%20var.%20Olivastra%20seggianese%20)%20as%20Antioxidant%20Source%20for%20Biomedicine&rft.jtitle=International%20journal%20of%20molecular%20sciences&rft.au=De%20la%20Ossa,%20Jose%20Gustavo&rft.date=2019-11-25&rft.volume=20&rft.issue=23&rft.spage=5918&rft.pages=5918-&rft.issn=1422-0067&rft.eissn=1422-0067&rft_id=info:doi/10.3390/ijms20235918&rft_dat=%3Cproquest_pubme%3E2548650851%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2548650851&rft_id=info:pmid/31775339&rfr_iscdi=true |