Anti-Inflammatory and Antioxidant Properties of Dehydrated Potato-Derived Bioactive Compounds in Intestinal Cells
Inflammation and oxidative stress are always more recognized as responsible for chronic disease at the intestinal level. Currently, a growing interest is addressed to the discovery of diet-derived products which have anti-inflammatory and antioxidant properties. This work aims to characterize the ph...
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| Veröffentlicht in: | International journal of molecular sciences 2019-12, Vol.20 (23), p.6087 |
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| creator | Basilicata, Manuela Giovanna Pepe, Giacomo Rapa, Shara Francesca Merciai, Fabrizio Ostacolo, Carmine Manfra, Michele Di Sarno, Veronica Autore, Giuseppina De Vita, Daniela Marzocco, Stefania Campiglia, Pietro |
| description | Inflammation and oxidative stress are always more recognized as responsible for chronic disease at the intestinal level. Currently, a growing interest is addressed to the discovery of diet-derived products which have anti-inflammatory and antioxidant properties. This work aims to characterize the pharmacological potential of dehydrated potatoes. For this purpose, a simulated gastrointestinal digestion was carried out. The bioaccessible peptides were fractionated on the basis of their molecular weight and tested on intestinal epithelial cells (IEC-6) under oxidative and inflammatory conditions. Our results demonstrate that the tested peptide fractions were able to significantly inhibit tumor necrosis factor-α release and cycloxygenase-2 and inducible nitric oxide synthase expression. The tested peptides also showed significant antioxidant activity, being able to both reduce reactive oxygen species (ROS) release, also from mitochondria, and nitrotyrosine formation, and increase the antioxidant response by heme oxygenase-1 and superoxide dismutase expression. Moreover, the peptide fractions were able to significantly increase the wound repair in IEC-6. The obtained results indicate the anti-inflammatory and antioxidant potential of dehydrated potatoes at the intestinal level. |
| doi_str_mv | 10.3390/ijms20236087 |
| format | Article |
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Currently, a growing interest is addressed to the discovery of diet-derived products which have anti-inflammatory and antioxidant properties. This work aims to characterize the pharmacological potential of dehydrated potatoes. For this purpose, a simulated gastrointestinal digestion was carried out. The bioaccessible peptides were fractionated on the basis of their molecular weight and tested on intestinal epithelial cells (IEC-6) under oxidative and inflammatory conditions. Our results demonstrate that the tested peptide fractions were able to significantly inhibit tumor necrosis factor-α release and cycloxygenase-2 and inducible nitric oxide synthase expression. The tested peptides also showed significant antioxidant activity, being able to both reduce reactive oxygen species (ROS) release, also from mitochondria, and nitrotyrosine formation, and increase the antioxidant response by heme oxygenase-1 and superoxide dismutase expression. Moreover, the peptide fractions were able to significantly increase the wound repair in IEC-6. The obtained results indicate the anti-inflammatory and antioxidant potential of dehydrated potatoes at the intestinal level.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms20236087</identifier><identifier>PMID: 31816826</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Amino acids ; Animals ; Anti-Inflammatory Agents - pharmacology ; Antioxidants ; Antioxidants - pharmacology ; Bioactive compounds ; Cancer ; Cell Line ; Cell Movement - drug effects ; Cell Survival - drug effects ; Cyclooxygenase 2 - metabolism ; Cytokines ; Dehydration ; Desiccation ; Digestion - drug effects ; Disease ; Enzymes ; Epithelial cells ; Epithelium ; Fatty acids ; Gastrointestinal Tract - physiology ; Heme ; Heme oxygenase (decyclizing) ; Heme Oxygenase-1 - metabolism ; Inflammation ; Inflammatory bowel disease ; Interferons - pharmacology ; Intestine ; Intestines - cytology ; Lipopolysaccharides - pharmacology ; Mitochondria ; Molecular weight ; Nitric oxide ; Nitric Oxide Synthase Type II - metabolism ; Nitric-oxide synthase ; Nitrotyrosine ; Oxidative stress ; Oxygenase ; Pathogenesis ; Peptides ; Peptides - metabolism ; Phytochemicals - pharmacology ; Potatoes ; Proteins ; Rats ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Solanum tuberosum - chemistry ; Stress, Mechanical ; Superoxide dismutase ; Superoxide Dismutase - metabolism ; Tumor Necrosis Factor-alpha - metabolism ; Tumor necrosis factor-α ; Tyrosine - analogs & derivatives ; Tyrosine - metabolism ; Wound healing</subject><ispartof>International journal of molecular sciences, 2019-12, Vol.20 (23), p.6087</ispartof><rights>2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 by the authors. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-64c295a819fb5c6ae1bffed5065ebd473cac5031c60634078c6d4adf572d1fd13</citedby><cites>FETCH-LOGICAL-c412t-64c295a819fb5c6ae1bffed5065ebd473cac5031c60634078c6d4adf572d1fd13</cites><orcidid>0000-0001-8423-9489 ; 0000-0002-1069-2181 ; 0000-0003-2333-1630 ; 0000-0003-3715-8680</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928682/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928682/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31816826$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Basilicata, Manuela Giovanna</creatorcontrib><creatorcontrib>Pepe, Giacomo</creatorcontrib><creatorcontrib>Rapa, Shara Francesca</creatorcontrib><creatorcontrib>Merciai, Fabrizio</creatorcontrib><creatorcontrib>Ostacolo, Carmine</creatorcontrib><creatorcontrib>Manfra, Michele</creatorcontrib><creatorcontrib>Di Sarno, Veronica</creatorcontrib><creatorcontrib>Autore, Giuseppina</creatorcontrib><creatorcontrib>De Vita, Daniela</creatorcontrib><creatorcontrib>Marzocco, Stefania</creatorcontrib><creatorcontrib>Campiglia, Pietro</creatorcontrib><title>Anti-Inflammatory and Antioxidant Properties of Dehydrated Potato-Derived Bioactive Compounds in Intestinal Cells</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Inflammation and oxidative stress are always more recognized as responsible for chronic disease at the intestinal level. Currently, a growing interest is addressed to the discovery of diet-derived products which have anti-inflammatory and antioxidant properties. This work aims to characterize the pharmacological potential of dehydrated potatoes. For this purpose, a simulated gastrointestinal digestion was carried out. The bioaccessible peptides were fractionated on the basis of their molecular weight and tested on intestinal epithelial cells (IEC-6) under oxidative and inflammatory conditions. Our results demonstrate that the tested peptide fractions were able to significantly inhibit tumor necrosis factor-α release and cycloxygenase-2 and inducible nitric oxide synthase expression. The tested peptides also showed significant antioxidant activity, being able to both reduce reactive oxygen species (ROS) release, also from mitochondria, and nitrotyrosine formation, and increase the antioxidant response by heme oxygenase-1 and superoxide dismutase expression. 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The obtained results indicate the anti-inflammatory and antioxidant potential of dehydrated potatoes at the intestinal level.</description><subject>Amino acids</subject><subject>Animals</subject><subject>Anti-Inflammatory Agents - pharmacology</subject><subject>Antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>Bioactive compounds</subject><subject>Cancer</subject><subject>Cell Line</subject><subject>Cell Movement - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Cyclooxygenase 2 - metabolism</subject><subject>Cytokines</subject><subject>Dehydration</subject><subject>Desiccation</subject><subject>Digestion - drug effects</subject><subject>Disease</subject><subject>Enzymes</subject><subject>Epithelial cells</subject><subject>Epithelium</subject><subject>Fatty acids</subject><subject>Gastrointestinal Tract - physiology</subject><subject>Heme</subject><subject>Heme oxygenase (decyclizing)</subject><subject>Heme Oxygenase-1 - metabolism</subject><subject>Inflammation</subject><subject>Inflammatory bowel disease</subject><subject>Interferons - pharmacology</subject><subject>Intestine</subject><subject>Intestines - cytology</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Mitochondria</subject><subject>Molecular weight</subject><subject>Nitric oxide</subject><subject>Nitric Oxide Synthase Type II - metabolism</subject><subject>Nitric-oxide synthase</subject><subject>Nitrotyrosine</subject><subject>Oxidative stress</subject><subject>Oxygenase</subject><subject>Pathogenesis</subject><subject>Peptides</subject><subject>Peptides - metabolism</subject><subject>Phytochemicals - pharmacology</subject><subject>Potatoes</subject><subject>Proteins</subject><subject>Rats</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Solanum tuberosum - chemistry</subject><subject>Stress, Mechanical</subject><subject>Superoxide dismutase</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><subject>Tumor necrosis factor-α</subject><subject>Tyrosine - 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Currently, a growing interest is addressed to the discovery of diet-derived products which have anti-inflammatory and antioxidant properties. This work aims to characterize the pharmacological potential of dehydrated potatoes. For this purpose, a simulated gastrointestinal digestion was carried out. The bioaccessible peptides were fractionated on the basis of their molecular weight and tested on intestinal epithelial cells (IEC-6) under oxidative and inflammatory conditions. Our results demonstrate that the tested peptide fractions were able to significantly inhibit tumor necrosis factor-α release and cycloxygenase-2 and inducible nitric oxide synthase expression. The tested peptides also showed significant antioxidant activity, being able to both reduce reactive oxygen species (ROS) release, also from mitochondria, and nitrotyrosine formation, and increase the antioxidant response by heme oxygenase-1 and superoxide dismutase expression. 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| ispartof | International journal of molecular sciences, 2019-12, Vol.20 (23), p.6087 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; MDPI - Multidisciplinary Digital Publishing Institute; PubMed Central |
| subjects | Amino acids Animals Anti-Inflammatory Agents - pharmacology Antioxidants Antioxidants - pharmacology Bioactive compounds Cancer Cell Line Cell Movement - drug effects Cell Survival - drug effects Cyclooxygenase 2 - metabolism Cytokines Dehydration Desiccation Digestion - drug effects Disease Enzymes Epithelial cells Epithelium Fatty acids Gastrointestinal Tract - physiology Heme Heme oxygenase (decyclizing) Heme Oxygenase-1 - metabolism Inflammation Inflammatory bowel disease Interferons - pharmacology Intestine Intestines - cytology Lipopolysaccharides - pharmacology Mitochondria Molecular weight Nitric oxide Nitric Oxide Synthase Type II - metabolism Nitric-oxide synthase Nitrotyrosine Oxidative stress Oxygenase Pathogenesis Peptides Peptides - metabolism Phytochemicals - pharmacology Potatoes Proteins Rats Reactive oxygen species Reactive Oxygen Species - metabolism Solanum tuberosum - chemistry Stress, Mechanical Superoxide dismutase Superoxide Dismutase - metabolism Tumor Necrosis Factor-alpha - metabolism Tumor necrosis factor-α Tyrosine - analogs & derivatives Tyrosine - metabolism Wound healing |
| title | Anti-Inflammatory and Antioxidant Properties of Dehydrated Potato-Derived Bioactive Compounds in Intestinal Cells |
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