Upregulation of VEGF and PEDF in Placentas of Women with Lower Extremity Venous Insufficiency during Pregnancy and Its Implication in Villous Calcification
Pregnancy is a period in a woman’s life in which changes can occur that affect different physiological processes. Common conditions during this period include vascular changes, such as lower extremity venous insufficiency (VI). This is an observational, analytical, and prospective cohort study in wh...
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| Veröffentlicht in: | BioMed research international 2019, Vol.2019 (2019), p.1-8 |
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| creator | García-Honduvilla, Natalio Buján, Julia Sainz, Felipe Coca, Santiago Bravo, Coral Romero, Beatriz Asúnsolo, Ángel Saez, Miguel Ángel Ortega, Miguel A Alvarez-Mon, Melchor |
| description | Pregnancy is a period in a woman’s life in which changes can occur that affect different physiological processes. Common conditions during this period include vascular changes, such as lower extremity venous insufficiency (VI). This is an observational, analytical, and prospective cohort study in which 114 pregnant women were analyzed, of which 62 were clinically diagnosed with VI. In parallel, 52 control patients without VI (HC) were studied. The aim of this study was to observe changes in angiogenesis and inflammation markers as well as the presence of calcium deposits. The expression of vascular endothelial growth factor (VEGF), transforming growth factor-β (TGF-β), and pigment epithelium-derived factor (PEDF) was analyzed by immunohistochemistry and RT-qPCR. The presence of calcium deposits was revealed using the von Kossa method. In the placentas of mothers with VI, gene expression of VEGF (34.575 [32.380–36.720] VI vs 32.965 [30.580–36.320] HC) and PEDF (25.417 [24.459–27.675] VI vs 24.400 [23.102–30.223] HC) significantly increased, as was protein expression in the placental villi. An increase in calcium deposits was observed in the placentas of women with VI (72.58% VI/53.84% HC). This study revealed the existence of cellular damage in the placental villi of mothers with VI with tissue implications such as increased calcification. |
| doi_str_mv | 10.1155/2019/5320902 |
| format | Article |
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Common conditions during this period include vascular changes, such as lower extremity venous insufficiency (VI). This is an observational, analytical, and prospective cohort study in which 114 pregnant women were analyzed, of which 62 were clinically diagnosed with VI. In parallel, 52 control patients without VI (HC) were studied. The aim of this study was to observe changes in angiogenesis and inflammation markers as well as the presence of calcium deposits. The expression of vascular endothelial growth factor (VEGF), transforming growth factor-β (TGF-β), and pigment epithelium-derived factor (PEDF) was analyzed by immunohistochemistry and RT-qPCR. The presence of calcium deposits was revealed using the von Kossa method. In the placentas of mothers with VI, gene expression of VEGF (34.575 [32.380–36.720] VI vs 32.965 [30.580–36.320] HC) and PEDF (25.417 [24.459–27.675] VI vs 24.400 [23.102–30.223] HC) significantly increased, as was protein expression in the placental villi. An increase in calcium deposits was observed in the placentas of women with VI (72.58% VI/53.84% HC). This study revealed the existence of cellular damage in the placental villi of mothers with VI with tissue implications such as increased calcification.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2019/5320902</identifier><identifier>PMID: 31886225</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Adolescent ; Adult ; Alcohol ; Analysis ; Angiogenesis ; Antibiotics ; Binding sites ; Calcification ; Calcinosis - metabolism ; Calcinosis - physiopathology ; Calcium ; Classification ; Deposits ; Epithelium ; Eye Proteins - analysis ; Eye Proteins - chemistry ; Eye Proteins - metabolism ; Female ; Gene expression ; Growth factors ; Humans ; Immunohistochemistry ; Laboratories ; Lower Extremity - physiopathology ; Nerve Growth Factors - analysis ; Nerve Growth Factors - chemistry ; Nerve Growth Factors - metabolism ; Pathology ; Phosphatase ; Pigment epithelium-derived factor ; Placenta ; Placenta - blood supply ; Placenta - chemistry ; Placenta - pathology ; Preeclampsia ; Pregnancy ; Pregnancy Complications, Cardiovascular - metabolism ; Pregnancy Complications, Cardiovascular - physiopathology ; Pregnant women ; Prospective Studies ; Serpins - analysis ; Serpins - chemistry ; Serpins - metabolism ; Studies ; Temperature ; Transforming growth factor-b ; Up-Regulation ; Vascular endothelial growth factor ; Vascular Endothelial Growth Factor A - analysis ; Vascular Endothelial Growth Factor A - chemistry ; Vascular Endothelial Growth Factor A - metabolism ; Venous insufficiency ; Venous Insufficiency - metabolism ; Venous Insufficiency - physiopathology ; Womens health ; Young Adult</subject><ispartof>BioMed research international, 2019, Vol.2019 (2019), p.1-8</ispartof><rights>Copyright © 2019 Miguel A Ortega et al.</rights><rights>COPYRIGHT 2019 John Wiley & Sons, Inc.</rights><rights>Copyright © 2019 Miguel A Ortega et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. http://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2019 Miguel A Ortega et al. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-b4960c88ead69bb125958c9e1ca3abcb82367320ed7be347b735e1fc86be6f133</citedby><cites>FETCH-LOGICAL-c499t-b4960c88ead69bb125958c9e1ca3abcb82367320ed7be347b735e1fc86be6f133</cites><orcidid>0000-0003-0904-6198 ; 0000-0003-1309-7510 ; 0000-0003-2419-2476 ; 0000-0002-4705-8651</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925773/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925773/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,4024,27923,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31886225$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Naruse, Katsuhiko</contributor><contributor>Katsuhiko Naruse</contributor><creatorcontrib>García-Honduvilla, Natalio</creatorcontrib><creatorcontrib>Buján, Julia</creatorcontrib><creatorcontrib>Sainz, Felipe</creatorcontrib><creatorcontrib>Coca, Santiago</creatorcontrib><creatorcontrib>Bravo, Coral</creatorcontrib><creatorcontrib>Romero, Beatriz</creatorcontrib><creatorcontrib>Asúnsolo, Ángel</creatorcontrib><creatorcontrib>Saez, Miguel Ángel</creatorcontrib><creatorcontrib>Ortega, Miguel A</creatorcontrib><creatorcontrib>Alvarez-Mon, Melchor</creatorcontrib><title>Upregulation of VEGF and PEDF in Placentas of Women with Lower Extremity Venous Insufficiency during Pregnancy and Its Implication in Villous Calcification</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Pregnancy is a period in a woman’s life in which changes can occur that affect different physiological processes. 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An increase in calcium deposits was observed in the placentas of women with VI (72.58% VI/53.84% HC). 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analysis</subject><subject>Vascular Endothelial Growth Factor A - chemistry</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>Venous insufficiency</subject><subject>Venous Insufficiency - metabolism</subject><subject>Venous Insufficiency - physiopathology</subject><subject>Womens health</subject><subject>Young Adult</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkk-PEyEYxidG427WvXk2JF5MtO4AMwxcTDa1XZs0sQe3HgnDvLRsGKjMjLWfxS8rk9auepILhPfH8_D-ybKXOH-PcVnekByLm5KSXOTkSXZJKC4mDBf46flM6UV23XUPeVocs1yw59kFxZwzQsrL7Of9LsJmcKq3waNg0Hp2N0fKN2g1-zhH1qOVUxp8r7ox-jW04NHe9lu0DHuIaPajj9Da_oDW4MPQoYXvBmOstuD1ATVDtH6DVsnDq_FiVF70CWt3zuqjazJZW-fG11PltDWnwIvsmVGug-vTfpXdz2dfpp8my893i-ntcqILIfpJXQiWa85BNUzUNSalKLkWgLWiqtY1J5RVqUTQVDXQoqorWgI2mrMamEkFuso-HHV3Q91CM2YblZO7aFsVDzIoK_-OeLuVm_BdMkHKqhoF3pwEYvg2QNfL1nYanFMeUlaS0NQLUmHCEvr6H_QhDNGn9BJFBOMFwdUjtVEOpPUmJF89isrb1F7GaWpeot4dKR1D10Uw5y_jXI7jIcfxkKfxSPirP9M8w7-HIQFvj8DW-kbt7X_KQWLAqEd67AAj9BfwXsy7</recordid><startdate>2019</startdate><enddate>2019</enddate><creator>García-Honduvilla, Natalio</creator><creator>Buján, Julia</creator><creator>Sainz, Felipe</creator><creator>Coca, Santiago</creator><creator>Bravo, Coral</creator><creator>Romero, Beatriz</creator><creator>Asúnsolo, Ángel</creator><creator>Saez, Miguel Ángel</creator><creator>Ortega, Miguel A</creator><creator>Alvarez-Mon, Melchor</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-0904-6198</orcidid><orcidid>https://orcid.org/0000-0003-1309-7510</orcidid><orcidid>https://orcid.org/0000-0003-2419-2476</orcidid><orcidid>https://orcid.org/0000-0002-4705-8651</orcidid></search><sort><creationdate>2019</creationdate><title>Upregulation of VEGF and PEDF in Placentas of Women with Lower Extremity Venous Insufficiency during Pregnancy and Its Implication in Villous Calcification</title><author>García-Honduvilla, Natalio ; Buján, Julia ; Sainz, Felipe ; Coca, Santiago ; Bravo, Coral ; Romero, Beatriz ; Asúnsolo, Ángel ; Saez, Miguel Ángel ; Ortega, Miguel A ; Alvarez-Mon, Melchor</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-b4960c88ead69bb125958c9e1ca3abcb82367320ed7be347b735e1fc86be6f133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Alcohol</topic><topic>Analysis</topic><topic>Angiogenesis</topic><topic>Antibiotics</topic><topic>Binding sites</topic><topic>Calcification</topic><topic>Calcinosis - 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Common conditions during this period include vascular changes, such as lower extremity venous insufficiency (VI). This is an observational, analytical, and prospective cohort study in which 114 pregnant women were analyzed, of which 62 were clinically diagnosed with VI. In parallel, 52 control patients without VI (HC) were studied. The aim of this study was to observe changes in angiogenesis and inflammation markers as well as the presence of calcium deposits. The expression of vascular endothelial growth factor (VEGF), transforming growth factor-β (TGF-β), and pigment epithelium-derived factor (PEDF) was analyzed by immunohistochemistry and RT-qPCR. The presence of calcium deposits was revealed using the von Kossa method. In the placentas of mothers with VI, gene expression of VEGF (34.575 [32.380–36.720] VI vs 32.965 [30.580–36.320] HC) and PEDF (25.417 [24.459–27.675] VI vs 24.400 [23.102–30.223] HC) significantly increased, as was protein expression in the placental villi. 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| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6925773 |
| source | MEDLINE; PubMed Central Open Access; Wiley-Blackwell Open Access Titles; PubMed Central; Alma/SFX Local Collection |
| subjects | Adolescent Adult Alcohol Analysis Angiogenesis Antibiotics Binding sites Calcification Calcinosis - metabolism Calcinosis - physiopathology Calcium Classification Deposits Epithelium Eye Proteins - analysis Eye Proteins - chemistry Eye Proteins - metabolism Female Gene expression Growth factors Humans Immunohistochemistry Laboratories Lower Extremity - physiopathology Nerve Growth Factors - analysis Nerve Growth Factors - chemistry Nerve Growth Factors - metabolism Pathology Phosphatase Pigment epithelium-derived factor Placenta Placenta - blood supply Placenta - chemistry Placenta - pathology Preeclampsia Pregnancy Pregnancy Complications, Cardiovascular - metabolism Pregnancy Complications, Cardiovascular - physiopathology Pregnant women Prospective Studies Serpins - analysis Serpins - chemistry Serpins - metabolism Studies Temperature Transforming growth factor-b Up-Regulation Vascular endothelial growth factor Vascular Endothelial Growth Factor A - analysis Vascular Endothelial Growth Factor A - chemistry Vascular Endothelial Growth Factor A - metabolism Venous insufficiency Venous Insufficiency - metabolism Venous Insufficiency - physiopathology Womens health Young Adult |
| title | Upregulation of VEGF and PEDF in Placentas of Women with Lower Extremity Venous Insufficiency during Pregnancy and Its Implication in Villous Calcification |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T10%3A52%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Upregulation%20of%20VEGF%20and%20PEDF%20in%20Placentas%20of%20Women%20with%20Lower%20Extremity%20Venous%20Insufficiency%20during%20Pregnancy%20and%20Its%20Implication%20in%20Villous%20Calcification&rft.jtitle=BioMed%20research%20international&rft.au=Garc%C3%ADa-Honduvilla,%20Natalio&rft.date=2019&rft.volume=2019&rft.issue=2019&rft.spage=1&rft.epage=8&rft.pages=1-8&rft.issn=2314-6133&rft.eissn=2314-6141&rft_id=info:doi/10.1155/2019/5320902&rft_dat=%3Cgale_pubme%3EA614683225%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2329684217&rft_id=info:pmid/31886225&rft_galeid=A614683225&rfr_iscdi=true |