Silencing of zinc finger protein 703 inhibits medullary thyroid carcinoma cell proliferation in vitro and in vivo
Zinc finger protein 703 (ZNF703) is a new member of the zinc finger protein family of transcription factors that plays an important role during embryogenesis in metazoans. The overexpression of ZNF703 contributes to tumorigenesis and progression of a number of malignancies by activating the Akt/mamm...
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| Veröffentlicht in: | Oncology letters 2020-01, Vol.19 (1), p.943-951 |
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| creator | Yang, Xiaolin Liu, Geling Li, Weijuan Zang, Luyang Li, Ding Wang, Qianqian Yu, Fang Xiang, Xiuxiu |
| description | Zinc finger protein 703 (ZNF703) is a new member of the zinc finger protein family of transcription factors that plays an important role during embryogenesis in metazoans. The overexpression of ZNF703 contributes to tumorigenesis and progression of a number of malignancies by activating the Akt/mammalian target of rapamycin (mTOR) signaling pathway. This pathway is activated in medullary thyroid cancer (MTC), but its mechanism of action is not yet fully understood. The aim of the present study was to examine the role of ZNF703 and its association with Akt/mTOR activation in MTC. The present study used the phosphorylation of Akt1 protein at serine 473 (pAkt
) as an indicator of signaling activation. Immunohistochemistry (IHC) staining and western blot analyses were performed in order to examine the expression of ZNF703 in 34 cases of MTC and 12 cases of corresponding normal thyroid tissues. ZNF703 expression in MTC was significantly higher compared with the corresponding normal thyroid tissues (P |
| doi_str_mv | 10.3892/ol.2019.11153 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6924197</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A612031460</galeid><sourcerecordid>A612031460</sourcerecordid><originalsourceid>FETCH-LOGICAL-c513t-4c83a741a938a120da696f91d8857597329fe125bbcd2880ef3080ae7206c94b3</originalsourceid><addsrcrecordid>eNptks1rHCEYh6W0NGGbY69FKJReZuvHjKOXQghNWwj0kOQsjqM7Bkc3OrOQ_vV1ssk2W6oHffH5vV--ALzHaE25IF-iXxOExRpj3NBX4BS3glQYcfL6cG_rE3CW8x0qq2GYc_YWnFDMRUtQewrur503QbuwgdHC3y5oaIthEtymOBkXYIsodGFwnZsyHE0_e6_SA5yGhxRdD7VKRR1HBbXxflF5Z01Sk4uh6ODOTSlCFfq9sYvvwBurfDZnT-cK3F5-u7n4UV39-v7z4vyq0g2mU1VrTlVbYyUoV5igXjHBrMA9503biJYSYQ0mTdfpnnCOjKWII2VKWUyLuqMr8HXvdzt3JW1twpSUl9vkxpK_jMrJ45fgBrmJO8kEqXEJsAKfnxykeD-bPMnR5aVIFUycsySUUoZI0y7ox3_QuzinUMpbqBozRjH6S22UN9IFG0tcvTiV56yUSHHNFmr9H6rs3oxOx2Bs-bFjwacXgsEoPw05-nn5gXwMVntQp5hzMvbQDIzkMk8yernMk3ycp8J_eNnBA_08PfQPpanD1Q</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2334166310</pqid></control><display><type>article</type><title>Silencing of zinc finger protein 703 inhibits medullary thyroid carcinoma cell proliferation in vitro and in vivo</title><source>Spandidos Publications Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Yang, Xiaolin ; Liu, Geling ; Li, Weijuan ; Zang, Luyang ; Li, Ding ; Wang, Qianqian ; Yu, Fang ; Xiang, Xiuxiu</creator><creatorcontrib>Yang, Xiaolin ; Liu, Geling ; Li, Weijuan ; Zang, Luyang ; Li, Ding ; Wang, Qianqian ; Yu, Fang ; Xiang, Xiuxiu</creatorcontrib><description>Zinc finger protein 703 (ZNF703) is a new member of the zinc finger protein family of transcription factors that plays an important role during embryogenesis in metazoans. The overexpression of ZNF703 contributes to tumorigenesis and progression of a number of malignancies by activating the Akt/mammalian target of rapamycin (mTOR) signaling pathway. This pathway is activated in medullary thyroid cancer (MTC), but its mechanism of action is not yet fully understood. The aim of the present study was to examine the role of ZNF703 and its association with Akt/mTOR activation in MTC. The present study used the phosphorylation of Akt1 protein at serine 473 (pAkt
) as an indicator of signaling activation. Immunohistochemistry (IHC) staining and western blot analyses were performed in order to examine the expression of ZNF703 in 34 cases of MTC and 12 cases of corresponding normal thyroid tissues. ZNF703 expression in MTC was significantly higher compared with the corresponding normal thyroid tissues (P<0.05). Furthermore, expression of ZNF703 was associated with tumor size, lymph node metastasis and advanced stage of disease. IHC also demonstrated that the level of ZNF703 was positively correlated with p-Akt
in the 34 cases of MTC. The human MTC cell line TT was selected for further investigation as TT cells exhibit Akt/mTOR activation. The biological effects of silencing ZNF703 in TT cells on proliferation and apoptosis, both
and
were investigated in the present study. ZNF703 silencing inhibited the proliferation of TT cells
and inhibited xenograft tumor growth
. These effects were accompanied by the substantial decrease of pAkt
and the induction of p53 protein. These results demonstrate that ZNF703 may play a relevant role in MTC due to its association with the Akt/mTOR signaling pathway.</description><identifier>ISSN: 1792-1074</identifier><identifier>EISSN: 1792-1082</identifier><identifier>DOI: 10.3892/ol.2019.11153</identifier><identifier>PMID: 31897207</identifier><language>eng</language><publisher>Greece: Spandidos Publications</publisher><subject>Biotechnology ; Cancer metastasis ; Carcinoma ; DNA binding proteins ; EDTA ; Ethanol ; Gene expression ; Immunohistochemistry ; Kinases ; Oncology ; Pharmaceutical industry ; Proteins ; Scientific equipment industry ; Serine ; Studies ; Thyroid cancer ; Thyroid diseases ; Thyroid gland ; Tumor proteins ; Tumors</subject><ispartof>Oncology letters, 2020-01, Vol.19 (1), p.943-951</ispartof><rights>Copyright: © Yang et al.</rights><rights>COPYRIGHT 2020 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2020</rights><rights>Copyright: © Yang et al. 2020</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c513t-4c83a741a938a120da696f91d8857597329fe125bbcd2880ef3080ae7206c94b3</citedby><cites>FETCH-LOGICAL-c513t-4c83a741a938a120da696f91d8857597329fe125bbcd2880ef3080ae7206c94b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6924197/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6924197/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31897207$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Xiaolin</creatorcontrib><creatorcontrib>Liu, Geling</creatorcontrib><creatorcontrib>Li, Weijuan</creatorcontrib><creatorcontrib>Zang, Luyang</creatorcontrib><creatorcontrib>Li, Ding</creatorcontrib><creatorcontrib>Wang, Qianqian</creatorcontrib><creatorcontrib>Yu, Fang</creatorcontrib><creatorcontrib>Xiang, Xiuxiu</creatorcontrib><title>Silencing of zinc finger protein 703 inhibits medullary thyroid carcinoma cell proliferation in vitro and in vivo</title><title>Oncology letters</title><addtitle>Oncol Lett</addtitle><description>Zinc finger protein 703 (ZNF703) is a new member of the zinc finger protein family of transcription factors that plays an important role during embryogenesis in metazoans. The overexpression of ZNF703 contributes to tumorigenesis and progression of a number of malignancies by activating the Akt/mammalian target of rapamycin (mTOR) signaling pathway. This pathway is activated in medullary thyroid cancer (MTC), but its mechanism of action is not yet fully understood. The aim of the present study was to examine the role of ZNF703 and its association with Akt/mTOR activation in MTC. The present study used the phosphorylation of Akt1 protein at serine 473 (pAkt
) as an indicator of signaling activation. Immunohistochemistry (IHC) staining and western blot analyses were performed in order to examine the expression of ZNF703 in 34 cases of MTC and 12 cases of corresponding normal thyroid tissues. ZNF703 expression in MTC was significantly higher compared with the corresponding normal thyroid tissues (P<0.05). Furthermore, expression of ZNF703 was associated with tumor size, lymph node metastasis and advanced stage of disease. IHC also demonstrated that the level of ZNF703 was positively correlated with p-Akt
in the 34 cases of MTC. The human MTC cell line TT was selected for further investigation as TT cells exhibit Akt/mTOR activation. The biological effects of silencing ZNF703 in TT cells on proliferation and apoptosis, both
and
were investigated in the present study. ZNF703 silencing inhibited the proliferation of TT cells
and inhibited xenograft tumor growth
. These effects were accompanied by the substantial decrease of pAkt
and the induction of p53 protein. These results demonstrate that ZNF703 may play a relevant role in MTC due to its association with the Akt/mTOR signaling pathway.</description><subject>Biotechnology</subject><subject>Cancer metastasis</subject><subject>Carcinoma</subject><subject>DNA binding proteins</subject><subject>EDTA</subject><subject>Ethanol</subject><subject>Gene expression</subject><subject>Immunohistochemistry</subject><subject>Kinases</subject><subject>Oncology</subject><subject>Pharmaceutical industry</subject><subject>Proteins</subject><subject>Scientific equipment industry</subject><subject>Serine</subject><subject>Studies</subject><subject>Thyroid cancer</subject><subject>Thyroid diseases</subject><subject>Thyroid gland</subject><subject>Tumor proteins</subject><subject>Tumors</subject><issn>1792-1074</issn><issn>1792-1082</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNptks1rHCEYh6W0NGGbY69FKJReZuvHjKOXQghNWwj0kOQsjqM7Bkc3OrOQ_vV1ssk2W6oHffH5vV--ALzHaE25IF-iXxOExRpj3NBX4BS3glQYcfL6cG_rE3CW8x0qq2GYc_YWnFDMRUtQewrur503QbuwgdHC3y5oaIthEtymOBkXYIsodGFwnZsyHE0_e6_SA5yGhxRdD7VKRR1HBbXxflF5Z01Sk4uh6ODOTSlCFfq9sYvvwBurfDZnT-cK3F5-u7n4UV39-v7z4vyq0g2mU1VrTlVbYyUoV5igXjHBrMA9503biJYSYQ0mTdfpnnCOjKWII2VKWUyLuqMr8HXvdzt3JW1twpSUl9vkxpK_jMrJ45fgBrmJO8kEqXEJsAKfnxykeD-bPMnR5aVIFUycsySUUoZI0y7ox3_QuzinUMpbqBozRjH6S22UN9IFG0tcvTiV56yUSHHNFmr9H6rs3oxOx2Bs-bFjwacXgsEoPw05-nn5gXwMVntQp5hzMvbQDIzkMk8yernMk3ycp8J_eNnBA_08PfQPpanD1Q</recordid><startdate>20200101</startdate><enddate>20200101</enddate><creator>Yang, Xiaolin</creator><creator>Liu, Geling</creator><creator>Li, Weijuan</creator><creator>Zang, Luyang</creator><creator>Li, Ding</creator><creator>Wang, Qianqian</creator><creator>Yu, Fang</creator><creator>Xiang, Xiuxiu</creator><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><general>D.A. Spandidos</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200101</creationdate><title>Silencing of zinc finger protein 703 inhibits medullary thyroid carcinoma cell proliferation in vitro and in vivo</title><author>Yang, Xiaolin ; Liu, Geling ; Li, Weijuan ; Zang, Luyang ; Li, Ding ; Wang, Qianqian ; Yu, Fang ; Xiang, Xiuxiu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-4c83a741a938a120da696f91d8857597329fe125bbcd2880ef3080ae7206c94b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Biotechnology</topic><topic>Cancer metastasis</topic><topic>Carcinoma</topic><topic>DNA binding proteins</topic><topic>EDTA</topic><topic>Ethanol</topic><topic>Gene expression</topic><topic>Immunohistochemistry</topic><topic>Kinases</topic><topic>Oncology</topic><topic>Pharmaceutical industry</topic><topic>Proteins</topic><topic>Scientific equipment industry</topic><topic>Serine</topic><topic>Studies</topic><topic>Thyroid cancer</topic><topic>Thyroid diseases</topic><topic>Thyroid gland</topic><topic>Tumor proteins</topic><topic>Tumors</topic><toplevel>online_resources</toplevel><creatorcontrib>Yang, Xiaolin</creatorcontrib><creatorcontrib>Liu, Geling</creatorcontrib><creatorcontrib>Li, Weijuan</creatorcontrib><creatorcontrib>Zang, Luyang</creatorcontrib><creatorcontrib>Li, Ding</creatorcontrib><creatorcontrib>Wang, Qianqian</creatorcontrib><creatorcontrib>Yu, Fang</creatorcontrib><creatorcontrib>Xiang, Xiuxiu</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oncology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Xiaolin</au><au>Liu, Geling</au><au>Li, Weijuan</au><au>Zang, Luyang</au><au>Li, Ding</au><au>Wang, Qianqian</au><au>Yu, Fang</au><au>Xiang, Xiuxiu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silencing of zinc finger protein 703 inhibits medullary thyroid carcinoma cell proliferation in vitro and in vivo</atitle><jtitle>Oncology letters</jtitle><addtitle>Oncol Lett</addtitle><date>2020-01-01</date><risdate>2020</risdate><volume>19</volume><issue>1</issue><spage>943</spage><epage>951</epage><pages>943-951</pages><issn>1792-1074</issn><eissn>1792-1082</eissn><abstract>Zinc finger protein 703 (ZNF703) is a new member of the zinc finger protein family of transcription factors that plays an important role during embryogenesis in metazoans. The overexpression of ZNF703 contributes to tumorigenesis and progression of a number of malignancies by activating the Akt/mammalian target of rapamycin (mTOR) signaling pathway. This pathway is activated in medullary thyroid cancer (MTC), but its mechanism of action is not yet fully understood. The aim of the present study was to examine the role of ZNF703 and its association with Akt/mTOR activation in MTC. The present study used the phosphorylation of Akt1 protein at serine 473 (pAkt
) as an indicator of signaling activation. Immunohistochemistry (IHC) staining and western blot analyses were performed in order to examine the expression of ZNF703 in 34 cases of MTC and 12 cases of corresponding normal thyroid tissues. ZNF703 expression in MTC was significantly higher compared with the corresponding normal thyroid tissues (P<0.05). Furthermore, expression of ZNF703 was associated with tumor size, lymph node metastasis and advanced stage of disease. IHC also demonstrated that the level of ZNF703 was positively correlated with p-Akt
in the 34 cases of MTC. The human MTC cell line TT was selected for further investigation as TT cells exhibit Akt/mTOR activation. The biological effects of silencing ZNF703 in TT cells on proliferation and apoptosis, both
and
were investigated in the present study. ZNF703 silencing inhibited the proliferation of TT cells
and inhibited xenograft tumor growth
. These effects were accompanied by the substantial decrease of pAkt
and the induction of p53 protein. These results demonstrate that ZNF703 may play a relevant role in MTC due to its association with the Akt/mTOR signaling pathway.</abstract><cop>Greece</cop><pub>Spandidos Publications</pub><pmid>31897207</pmid><doi>10.3892/ol.2019.11153</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | Spandidos Publications Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Biotechnology Cancer metastasis Carcinoma DNA binding proteins EDTA Ethanol Gene expression Immunohistochemistry Kinases Oncology Pharmaceutical industry Proteins Scientific equipment industry Serine Studies Thyroid cancer Thyroid diseases Thyroid gland Tumor proteins Tumors |
| title | Silencing of zinc finger protein 703 inhibits medullary thyroid carcinoma cell proliferation in vitro and in vivo |
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