An ultrasensitive fiveplex activity assay for cellular kinases

Protein kinases are enzymes whose abundance, protein-protein interactions, and posttranslational modifications together determine net signaling activity in cells. Large-scale data on cellular kinase activity are limited, because existing assays are cumbersome, poorly sensitive, low throughput, and r...

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Veröffentlicht in:	Scientific reports 2019-12, Vol.9 (1), p.19409-18, Article 19409
Hauptverfasser:	Smolko, Christian M., Janes, Kevin A.
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/2163 631/1647/2196/2197 631/1647/664/2089 631/553/1806 AKT protein Amino Acid Sequence Biocatalysis c-Jun protein Enterovirus - physiology Enzyme Assays - methods Genes, Reporter High-Throughput Screening Assays HT29 Cells Humanities and Social Sciences Humans Insulin - pharmacology JNK protein Kinases MAP kinase multidisciplinary Peptides - metabolism Phosphorylation Protein interaction Protein kinase Protein Kinases - chemistry Protein Kinases - metabolism Proteins Reproducibility of Results Science Science (multidisciplinary) Signal transduction Substrate Specificity Transcription factors Tumor Necrosis Factor-alpha - pharmacology
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