Classification of Cutibacterium acnes at phylotype level by MALDI-MS proteotyping
Cutibacterium acnes is a major commensal human skin bacteria. It is a producer of propionic acids that maintain skin acidic pH to inhibit the growth of pathogens. On the other hand, it is also associated with diseases such as acne vulgaris and sarcoidosis. C. acnes strains have been classified into...
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| Veröffentlicht in: | Proceedings of the Japan Academy Series B, 2019/12/11, Vol.95(10), pp.612-623 |
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| creator | TERAMOTO, Kanae OKUBO, Tatsuki YAMADA, Yoshihiro SEKIYA, Sadanori IWAMOTO, Shinichi TANAKA, Koichi |
| description | Cutibacterium acnes is a major commensal human skin bacteria. It is a producer of propionic acids that maintain skin acidic pH to inhibit the growth of pathogens. On the other hand, it is also associated with diseases such as acne vulgaris and sarcoidosis. C. acnes strains have been classified into six phylotypes using DNA-based approaches. Because several characteristic features of C. acnes vary according to the phylotype, the development of a practical method to identify these phylotypes is needed. For rapid identification of phylotypes for C. acnes strains, a matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) fingerprinting technique has been applied; however, some phylotypes have not been discriminated. We developed a high-throughput protein purification method to detect biomarker proteins by ultrafiltration. MALDI-MS proteotyping using profiling of identified biomarker peaks was applied for the classification of 24 strains of C. acnes, and these were successfully classified into the correct phylotypes. This is a promising method that allows the discrimination of C. acnes phylotypes independent of a DNA-based approach. |
| doi_str_mv | 10.2183/pjab.95.042 |
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It is a producer of propionic acids that maintain skin acidic pH to inhibit the growth of pathogens. On the other hand, it is also associated with diseases such as acne vulgaris and sarcoidosis. C. acnes strains have been classified into six phylotypes using DNA-based approaches. Because several characteristic features of C. acnes vary according to the phylotype, the development of a practical method to identify these phylotypes is needed. For rapid identification of phylotypes for C. acnes strains, a matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) fingerprinting technique has been applied; however, some phylotypes have not been discriminated. We developed a high-throughput protein purification method to detect biomarker proteins by ultrafiltration. MALDI-MS proteotyping using profiling of identified biomarker peaks was applied for the classification of 24 strains of C. acnes, and these were successfully classified into the correct phylotypes. This is a promising method that allows the discrimination of C. acnes phylotypes independent of a DNA-based approach.</description><identifier>ISSN: 0386-2208</identifier><identifier>ISSN: 0021-4280</identifier><identifier>EISSN: 1349-2896</identifier><identifier>DOI: 10.2183/pjab.95.042</identifier><identifier>PMID: 31827019</identifier><language>eng</language><publisher>Japan: The Japan Academy</publisher><subject>Acne ; Amino Acid Sequence ; Amino acids ; antitoxin ; Bacteria ; Bioinformatics ; Biological products ; Biomarkers ; Biomarkers - analysis ; Classification ; Cutibacterium acnes ; Deoxyribonucleic acid ; Discrimination ; DNA ; Drug resistance ; Fingerprinting ; Genomes ; High-Throughput Screening Assays ; Identification ; Instrument industry (Equipment) ; Ionization ; Ions ; MALDI-MS proteotyping ; Mass spectrometry ; Mass spectroscopy ; Methods ; Mutation ; Original ; Pathogens ; phylotype ; Propionibacteriaceae - classification ; Propionibacteriaceae - genetics ; Propionic acid ; Protein purification ; Proteins ; ribosomal protein ; Ribosomal Proteins - analysis ; Ribosomal Proteins - genetics ; Sarcoidosis ; Scientific imaging ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Strains (organisms) ; Ultrafiltration</subject><ispartof>Proceedings of the Japan Academy, Series B, 2019/12/11, Vol.95(10), pp.612-623</ispartof><rights>2019 The Japan Academy</rights><rights>COPYRIGHT 2019 The Japan Academy</rights><rights>Copyright The Japan Academy Dec 2019</rights><rights>2019 The Japan Academy 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-4b617436b5c3acf935db5f93ce75cbe59e83d6705b13960fd054a47402f61ec63</citedby><cites>FETCH-LOGICAL-c758t-4b617436b5c3acf935db5f93ce75cbe59e83d6705b13960fd054a47402f61ec63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6920080/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6920080/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1877,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31827019$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TERAMOTO, Kanae</creatorcontrib><creatorcontrib>OKUBO, Tatsuki</creatorcontrib><creatorcontrib>YAMADA, Yoshihiro</creatorcontrib><creatorcontrib>SEKIYA, Sadanori</creatorcontrib><creatorcontrib>IWAMOTO, Shinichi</creatorcontrib><creatorcontrib>TANAKA, Koichi</creatorcontrib><title>Classification of Cutibacterium acnes at phylotype level by MALDI-MS proteotyping</title><title>Proceedings of the Japan Academy</title><addtitle>Proc. Jpn. Acad., Ser. B</addtitle><description>Cutibacterium acnes is a major commensal human skin bacteria. It is a producer of propionic acids that maintain skin acidic pH to inhibit the growth of pathogens. On the other hand, it is also associated with diseases such as acne vulgaris and sarcoidosis. C. acnes strains have been classified into six phylotypes using DNA-based approaches. Because several characteristic features of C. acnes vary according to the phylotype, the development of a practical method to identify these phylotypes is needed. For rapid identification of phylotypes for C. acnes strains, a matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) fingerprinting technique has been applied; however, some phylotypes have not been discriminated. We developed a high-throughput protein purification method to detect biomarker proteins by ultrafiltration. MALDI-MS proteotyping using profiling of identified biomarker peaks was applied for the classification of 24 strains of C. acnes, and these were successfully classified into the correct phylotypes. This is a promising method that allows the discrimination of C. acnes phylotypes independent of a DNA-based approach.</description><subject>Acne</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>antitoxin</subject><subject>Bacteria</subject><subject>Bioinformatics</subject><subject>Biological products</subject><subject>Biomarkers</subject><subject>Biomarkers - analysis</subject><subject>Classification</subject><subject>Cutibacterium acnes</subject><subject>Deoxyribonucleic acid</subject><subject>Discrimination</subject><subject>DNA</subject><subject>Drug resistance</subject><subject>Fingerprinting</subject><subject>Genomes</subject><subject>High-Throughput Screening Assays</subject><subject>Identification</subject><subject>Instrument industry (Equipment)</subject><subject>Ionization</subject><subject>Ions</subject><subject>MALDI-MS proteotyping</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Methods</subject><subject>Mutation</subject><subject>Original</subject><subject>Pathogens</subject><subject>phylotype</subject><subject>Propionibacteriaceae - classification</subject><subject>Propionibacteriaceae - genetics</subject><subject>Propionic acid</subject><subject>Protein purification</subject><subject>Proteins</subject><subject>ribosomal protein</subject><subject>Ribosomal Proteins - analysis</subject><subject>Ribosomal Proteins - genetics</subject><subject>Sarcoidosis</subject><subject>Scientific imaging</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Strains (organisms)</subject><subject>Ultrafiltration</subject><issn>0386-2208</issn><issn>0021-4280</issn><issn>1349-2896</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptkt-L1DAQgIMo3nr65LsUfBGka340afMirOt5nuyhoj6HNJ3uZmmbmqQH-99fyt6tnkgggeSbb8jMIPSS4CUlFXs37nW9lHyJC_oILQgrZE4rKR6jBWaVyCnF1Rl6FsIeY0Z5RZ6iM0YqWmIiF-j7utMh2NYaHa0bMtdm6ynaWpsI3k59ps0AIdMxG3eHzsXDCFkHN9Bl9SG7Xm0-XuXXP7LRuwjzox22z9GTVncBXtyd5-jXp4uf68_55uvl1Xq1yU3Jq5gXtSBlwUTNDdOmlYw3NU-HgZKbGriEijWixLwmTArcNpgXuigLTFtBwAh2jt4fveNU99AYGKLXnRq97bU_KKetevgy2J3auhslJMW4wknw5k7g3e8JQlS9DQa6Tg_gpqBoqhZLNSvKhL7-B927yQ_pe4limEuanH-ore5A2aF1Ka-ZpWolCipSI8hMLf9DpdVAb40boLXp_kHA22OA8S4ED-3pjwSreQLUPAFKcpUmINGv_i7Lib1veQIuj8A-RL2FE6B9tCZlvpcledq_fVlJTvCH5D4RZqe9goHdAkzlw_I</recordid><startdate>20191211</startdate><enddate>20191211</enddate><creator>TERAMOTO, Kanae</creator><creator>OKUBO, Tatsuki</creator><creator>YAMADA, Yoshihiro</creator><creator>SEKIYA, Sadanori</creator><creator>IWAMOTO, Shinichi</creator><creator>TANAKA, Koichi</creator><general>The Japan Academy</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BVBZV</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20191211</creationdate><title>Classification of Cutibacterium acnes at phylotype level by MALDI-MS proteotyping</title><author>TERAMOTO, Kanae ; OKUBO, Tatsuki ; YAMADA, Yoshihiro ; SEKIYA, Sadanori ; IWAMOTO, Shinichi ; TANAKA, Koichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-4b617436b5c3acf935db5f93ce75cbe59e83d6705b13960fd054a47402f61ec63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acne</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>antitoxin</topic><topic>Bacteria</topic><topic>Bioinformatics</topic><topic>Biological products</topic><topic>Biomarkers</topic><topic>Biomarkers - analysis</topic><topic>Classification</topic><topic>Cutibacterium acnes</topic><topic>Deoxyribonucleic acid</topic><topic>Discrimination</topic><topic>DNA</topic><topic>Drug resistance</topic><topic>Fingerprinting</topic><topic>Genomes</topic><topic>High-Throughput Screening Assays</topic><topic>Identification</topic><topic>Instrument industry (Equipment)</topic><topic>Ionization</topic><topic>Ions</topic><topic>MALDI-MS proteotyping</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Methods</topic><topic>Mutation</topic><topic>Original</topic><topic>Pathogens</topic><topic>phylotype</topic><topic>Propionibacteriaceae - classification</topic><topic>Propionibacteriaceae - genetics</topic><topic>Propionic acid</topic><topic>Protein purification</topic><topic>Proteins</topic><topic>ribosomal protein</topic><topic>Ribosomal Proteins - analysis</topic><topic>Ribosomal Proteins - genetics</topic><topic>Sarcoidosis</topic><topic>Scientific imaging</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Strains (organisms)</topic><topic>Ultrafiltration</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TERAMOTO, Kanae</creatorcontrib><creatorcontrib>OKUBO, Tatsuki</creatorcontrib><creatorcontrib>YAMADA, Yoshihiro</creatorcontrib><creatorcontrib>SEKIYA, Sadanori</creatorcontrib><creatorcontrib>IWAMOTO, Shinichi</creatorcontrib><creatorcontrib>TANAKA, Koichi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>East & South Asia Database</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the Japan Academy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TERAMOTO, Kanae</au><au>OKUBO, Tatsuki</au><au>YAMADA, Yoshihiro</au><au>SEKIYA, Sadanori</au><au>IWAMOTO, Shinichi</au><au>TANAKA, Koichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Classification of Cutibacterium acnes at phylotype level by MALDI-MS proteotyping</atitle><jtitle>Proceedings of the Japan Academy</jtitle><addtitle>Proc. Jpn. Acad., Ser. B</addtitle><date>2019-12-11</date><risdate>2019</risdate><volume>95</volume><issue>10</issue><spage>612</spage><epage>623</epage><pages>612-623</pages><issn>0386-2208</issn><issn>0021-4280</issn><eissn>1349-2896</eissn><abstract>Cutibacterium acnes is a major commensal human skin bacteria. It is a producer of propionic acids that maintain skin acidic pH to inhibit the growth of pathogens. On the other hand, it is also associated with diseases such as acne vulgaris and sarcoidosis. C. acnes strains have been classified into six phylotypes using DNA-based approaches. Because several characteristic features of C. acnes vary according to the phylotype, the development of a practical method to identify these phylotypes is needed. For rapid identification of phylotypes for C. acnes strains, a matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) fingerprinting technique has been applied; however, some phylotypes have not been discriminated. We developed a high-throughput protein purification method to detect biomarker proteins by ultrafiltration. MALDI-MS proteotyping using profiling of identified biomarker peaks was applied for the classification of 24 strains of C. acnes, and these were successfully classified into the correct phylotypes. This is a promising method that allows the discrimination of C. acnes phylotypes independent of a DNA-based approach.</abstract><cop>Japan</cop><pub>The Japan Academy</pub><pmid>31827019</pmid><doi>10.2183/pjab.95.042</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acne Amino Acid Sequence Amino acids antitoxin Bacteria Bioinformatics Biological products Biomarkers Biomarkers - analysis Classification Cutibacterium acnes Deoxyribonucleic acid Discrimination DNA Drug resistance Fingerprinting Genomes High-Throughput Screening Assays Identification Instrument industry (Equipment) Ionization Ions MALDI-MS proteotyping Mass spectrometry Mass spectroscopy Methods Mutation Original Pathogens phylotype Propionibacteriaceae - classification Propionibacteriaceae - genetics Propionic acid Protein purification Proteins ribosomal protein Ribosomal Proteins - analysis Ribosomal Proteins - genetics Sarcoidosis Scientific imaging Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Strains (organisms) Ultrafiltration |
| title | Classification of Cutibacterium acnes at phylotype level by MALDI-MS proteotyping |
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