Decellularized Annulus Fibrosus Matrix/Chitosan Hybrid Hydrogels with Basic Fibroblast Growth Factor for Annulus Fibrosus Tissue Engineering
Low back pain caused by degenerative disc disease affects many people worldwide and brings huge economical burden. Thus, attentions have focused on annulus fibrosus (AF) tissue engineering for treatment of intervertebral disc degeneration. To engineer a functional replacement for the AF, it is impor...
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| Veröffentlicht in: | Tissue engineering. Part A 2019-12, Vol.25 (23-24), p.165-1613 |
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| container_title | Tissue engineering. Part A |
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| creator | Liu, Chen Jin, Zhongxing Ge, Xin Zhang, Yu Xu, Hongguang |
| description | Low back pain caused by degenerative disc disease affects many people worldwide and brings huge economical burden. Thus, attentions have focused on annulus fibrosus (AF) tissue engineering for treatment of intervertebral disc degeneration. To engineer a functional replacement for the AF, it is important to fabricate scaffolds that mimic the structural and mechanical properties of native tissue. AF-derived stem cells are promising seed cells for AF tissue engineering due to their tissue specificity. In the present study, decellularized AF matrix (DAFM)/chitosan hybrid hydrogels were fabricated using genipin as a crosslinker. AF stem cells were cultured on hydrogel scaffolds with or without basic fibroblast growth factor (bFGF), and cell proliferation, morphology, gene expression, and AF tissue synthesis were examined. Overall, more
collagen-I
,
collagen-II
, and
aggrecan
were secreted by AF stem cells grown on hydrogels with bFGF compared to those without. These results support the application of DAFM/chitosan hybrid hydrogels as an appropriate candidate for AF tissue engineering. Furthermore, incorporation of bFGF into hydrogels promoted AF-related tissue synthesis. |
| doi_str_mv | 10.1089/ten.tea.2018.0297 |
| format | Article |
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collagen-I
,
collagen-II
, and
aggrecan
were secreted by AF stem cells grown on hydrogels with bFGF compared to those without. These results support the application of DAFM/chitosan hybrid hydrogels as an appropriate candidate for AF tissue engineering. Furthermore, incorporation of bFGF into hydrogels promoted AF-related tissue synthesis.</description><identifier>ISSN: 1937-3341</identifier><identifier>EISSN: 1937-335X</identifier><identifier>DOI: 10.1089/ten.tea.2018.0297</identifier><identifier>PMID: 30929614</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc., publishers</publisher><subject>Aggrecan ; Cell differentiation ; Cell proliferation ; Chitosan ; Collagen ; Controlled release ; Cytology ; Degeneration ; Degenerative disc disease ; Extracellular matrix ; Fibroblast growth factor 2 ; Fibroblasts ; Gene expression ; Genipin ; Glycosaminoglycans ; Growth factors ; Hydrogels ; Immunogenicity ; Low back pain ; Mechanical properties ; Original ; Original Articles ; Stem cells ; Tissue engineering</subject><ispartof>Tissue engineering. Part A, 2019-12, Vol.25 (23-24), p.165-1613</ispartof><rights>Chen Liu et al. 2019; Published by Mary Ann Liebert, Inc.</rights><rights>Copyright Mary Ann Liebert, Inc. Dec 2019</rights><rights>Chen Liu et al. 2019; Published by Mary Ann Liebert, Inc. 2019 Chen Liu et al.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c523t-2d51127f4e59d01f0f6f63c5a96fc78e8aa6300261753c2a4ec0cbcb66823e843</citedby><cites>FETCH-LOGICAL-c523t-2d51127f4e59d01f0f6f63c5a96fc78e8aa6300261753c2a4ec0cbcb66823e843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30929614$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Chen</creatorcontrib><creatorcontrib>Jin, Zhongxing</creatorcontrib><creatorcontrib>Ge, Xin</creatorcontrib><creatorcontrib>Zhang, Yu</creatorcontrib><creatorcontrib>Xu, Hongguang</creatorcontrib><title>Decellularized Annulus Fibrosus Matrix/Chitosan Hybrid Hydrogels with Basic Fibroblast Growth Factor for Annulus Fibrosus Tissue Engineering</title><title>Tissue engineering. Part A</title><addtitle>Tissue Eng Part A</addtitle><description>Low back pain caused by degenerative disc disease affects many people worldwide and brings huge economical burden. Thus, attentions have focused on annulus fibrosus (AF) tissue engineering for treatment of intervertebral disc degeneration. To engineer a functional replacement for the AF, it is important to fabricate scaffolds that mimic the structural and mechanical properties of native tissue. AF-derived stem cells are promising seed cells for AF tissue engineering due to their tissue specificity. In the present study, decellularized AF matrix (DAFM)/chitosan hybrid hydrogels were fabricated using genipin as a crosslinker. AF stem cells were cultured on hydrogel scaffolds with or without basic fibroblast growth factor (bFGF), and cell proliferation, morphology, gene expression, and AF tissue synthesis were examined. Overall, more
collagen-I
,
collagen-II
, and
aggrecan
were secreted by AF stem cells grown on hydrogels with bFGF compared to those without. These results support the application of DAFM/chitosan hybrid hydrogels as an appropriate candidate for AF tissue engineering. Furthermore, incorporation of bFGF into hydrogels promoted AF-related tissue synthesis.</description><subject>Aggrecan</subject><subject>Cell differentiation</subject><subject>Cell proliferation</subject><subject>Chitosan</subject><subject>Collagen</subject><subject>Controlled release</subject><subject>Cytology</subject><subject>Degeneration</subject><subject>Degenerative disc disease</subject><subject>Extracellular matrix</subject><subject>Fibroblast growth factor 2</subject><subject>Fibroblasts</subject><subject>Gene expression</subject><subject>Genipin</subject><subject>Glycosaminoglycans</subject><subject>Growth factors</subject><subject>Hydrogels</subject><subject>Immunogenicity</subject><subject>Low back pain</subject><subject>Mechanical properties</subject><subject>Original</subject><subject>Original Articles</subject><subject>Stem cells</subject><subject>Tissue engineering</subject><issn>1937-3341</issn><issn>1937-335X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>1-M</sourceid><recordid>eNqNkU1vEzEQhi0EoiXlB3BBK3HhktQfu_64ILWhaZGKuLQSN8vrnU1cbexieynlN_RH16uUiI8Lh9FY42dezcyL0BuCFwRLdZzBLzKYBcVELjBV4hk6JIqJOWPN1-f7d00O0KuUbjDmmAvxEh0wrKjipD5EDx_BwjCMg4nuJ3TViffjMKZq5doYUnl8Njm6H8fLjcshGV9d3LfRdSV1MaxhSNWdy5vq1CRnd03tYFKuzmO4K_WVsTnEqi_xj_KVS2mE6syvnQeIzq-P0IveDAleP-UZul6dXS0v5pdfzj8tTy7ntqEsz2nXEEJFX0OjOkx63POeM9sYxXsrJEhjOMOYciIaZqmpwWLb2pZzSRnIms3Qh53u7dhuobPgczSDvo1ua-K9DsbpP3-82-h1-K65IoqWGWbo_ZNADN9GSFlvXZruaDyEMWlaHBGESyUL-u4v9CaM0Zf1NGVUFE5iXiiyo2y5TYrQ74chWE9e6-J1CaMnr_Xkdel5-_sW-45f5hZA7ICpbLwfHLQQ839IPwJCkL0E</recordid><startdate>20191201</startdate><enddate>20191201</enddate><creator>Liu, Chen</creator><creator>Jin, Zhongxing</creator><creator>Ge, Xin</creator><creator>Zhang, Yu</creator><creator>Xu, Hongguang</creator><general>Mary Ann Liebert, Inc., publishers</general><general>Mary Ann Liebert, Inc</general><scope>1-M</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20191201</creationdate><title>Decellularized Annulus Fibrosus Matrix/Chitosan Hybrid Hydrogels with Basic Fibroblast Growth Factor for Annulus Fibrosus Tissue Engineering</title><author>Liu, Chen ; Jin, Zhongxing ; Ge, Xin ; Zhang, Yu ; Xu, Hongguang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c523t-2d51127f4e59d01f0f6f63c5a96fc78e8aa6300261753c2a4ec0cbcb66823e843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Aggrecan</topic><topic>Cell differentiation</topic><topic>Cell proliferation</topic><topic>Chitosan</topic><topic>Collagen</topic><topic>Controlled release</topic><topic>Cytology</topic><topic>Degeneration</topic><topic>Degenerative disc disease</topic><topic>Extracellular matrix</topic><topic>Fibroblast growth factor 2</topic><topic>Fibroblasts</topic><topic>Gene expression</topic><topic>Genipin</topic><topic>Glycosaminoglycans</topic><topic>Growth factors</topic><topic>Hydrogels</topic><topic>Immunogenicity</topic><topic>Low back pain</topic><topic>Mechanical properties</topic><topic>Original</topic><topic>Original Articles</topic><topic>Stem cells</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Chen</creatorcontrib><creatorcontrib>Jin, Zhongxing</creatorcontrib><creatorcontrib>Ge, Xin</creatorcontrib><creatorcontrib>Zhang, Yu</creatorcontrib><creatorcontrib>Xu, Hongguang</creatorcontrib><collection>Mary Ann Liebert Online - Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Tissue engineering. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Chen</au><au>Jin, Zhongxing</au><au>Ge, Xin</au><au>Zhang, Yu</au><au>Xu, Hongguang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Decellularized Annulus Fibrosus Matrix/Chitosan Hybrid Hydrogels with Basic Fibroblast Growth Factor for Annulus Fibrosus Tissue Engineering</atitle><jtitle>Tissue engineering. Part A</jtitle><addtitle>Tissue Eng Part A</addtitle><date>2019-12-01</date><risdate>2019</risdate><volume>25</volume><issue>23-24</issue><spage>165</spage><epage>1613</epage><pages>165-1613</pages><issn>1937-3341</issn><eissn>1937-335X</eissn><abstract>Low back pain caused by degenerative disc disease affects many people worldwide and brings huge economical burden. Thus, attentions have focused on annulus fibrosus (AF) tissue engineering for treatment of intervertebral disc degeneration. To engineer a functional replacement for the AF, it is important to fabricate scaffolds that mimic the structural and mechanical properties of native tissue. AF-derived stem cells are promising seed cells for AF tissue engineering due to their tissue specificity. In the present study, decellularized AF matrix (DAFM)/chitosan hybrid hydrogels were fabricated using genipin as a crosslinker. AF stem cells were cultured on hydrogel scaffolds with or without basic fibroblast growth factor (bFGF), and cell proliferation, morphology, gene expression, and AF tissue synthesis were examined. Overall, more
collagen-I
,
collagen-II
, and
aggrecan
were secreted by AF stem cells grown on hydrogels with bFGF compared to those without. These results support the application of DAFM/chitosan hybrid hydrogels as an appropriate candidate for AF tissue engineering. Furthermore, incorporation of bFGF into hydrogels promoted AF-related tissue synthesis.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc., publishers</pub><pmid>30929614</pmid><doi>10.1089/ten.tea.2018.0297</doi><tpages>1449</tpages><oa>free_for_read</oa></addata></record> |
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| source | Alma/SFX Local Collection |
| subjects | Aggrecan Cell differentiation Cell proliferation Chitosan Collagen Controlled release Cytology Degeneration Degenerative disc disease Extracellular matrix Fibroblast growth factor 2 Fibroblasts Gene expression Genipin Glycosaminoglycans Growth factors Hydrogels Immunogenicity Low back pain Mechanical properties Original Original Articles Stem cells Tissue engineering |
| title | Decellularized Annulus Fibrosus Matrix/Chitosan Hybrid Hydrogels with Basic Fibroblast Growth Factor for Annulus Fibrosus Tissue Engineering |
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