Micro-computed tomography as a platform for exploring Drosophila development
Understanding how events at the molecular and cellular scales contribute to tissue form and function is key to uncovering the mechanisms driving animal development, physiology and disease. Elucidating these mechanisms has been enhanced through the study of model organisms and the use of sophisticate...
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| Veröffentlicht in: | Development (Cambridge) 2019-12, Vol.146 (23) |
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| creator | Schoborg, Todd A Smith, Samantha L Smith, Lauren N Morris, H Douglas Rusan, Nasser M |
| description | Understanding how events at the molecular and cellular scales contribute to tissue form and function is key to uncovering the mechanisms driving animal development, physiology and disease. Elucidating these mechanisms has been enhanced through the study of model organisms and the use of sophisticated genetic, biochemical and imaging tools. Here, we present an accessible method for non-invasive imaging of
at high resolution using micro-computed tomography (µ-CT). We show how rapid processing of intact animals, at any developmental stage, provides precise quantitative assessment of tissue size and morphology, and permits analysis of inter-organ relationships. We then use µ-CT imaging to study growth defects in the
brain through the characterization of
(
) and
(
), orthologs of the two most commonly mutated genes in human microcephaly patients. Our work demonstrates the power of combining µ-CT with traditional genetic, cellular and developmental biology tools available in model organisms to address novel biological mechanisms that control animal development and disease. |
| doi_str_mv | 10.1242/dev.176685 |
| format | Article |
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at high resolution using micro-computed tomography (µ-CT). We show how rapid processing of intact animals, at any developmental stage, provides precise quantitative assessment of tissue size and morphology, and permits analysis of inter-organ relationships. We then use µ-CT imaging to study growth defects in the
brain through the characterization of
(
) and
(
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at high resolution using micro-computed tomography (µ-CT). We show how rapid processing of intact animals, at any developmental stage, provides precise quantitative assessment of tissue size and morphology, and permits analysis of inter-organ relationships. We then use µ-CT imaging to study growth defects in the
brain through the characterization of
(
) and
(
), orthologs of the two most commonly mutated genes in human microcephaly patients. Our work demonstrates the power of combining µ-CT with traditional genetic, cellular and developmental biology tools available in model organisms to address novel biological mechanisms that control animal development and disease.</description><subject>Animals</subject><subject>Drosophila melanogaster</subject><subject>Drosophila Proteins - genetics</subject><subject>Drosophila Proteins - metabolism</subject><subject>Embryo, Nonmammalian - diagnostic imaging</subject><subject>Embryo, Nonmammalian - embryology</subject><subject>Humans</subject><subject>Microcephaly - diagnostic imaging</subject><subject>Microcephaly - embryology</subject><subject>Microcephaly - genetics</subject><subject>Mutation</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Techniques and Resources</subject><subject>X-Ray Microtomography</subject><issn>0950-1991</issn><issn>1477-9129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkMtKAzEUhoMotlY3PoBkKcLUXGZy2QhSr1Bxo-uQTDPtyMwkJjPFvr2R1qKbcxbn4_8PHwDnGE0xycn1wq6nmDMmigMwxjnnmcREHoIxkgXKsJR4BE5i_EAIUcb5MRhRzAkRgo7B_KUug8tK1_qhtwvYu9Ytg_arDdQRaugb3VcutDANaL9840LdLeFdcNH5Vd1omNpt43xru_4UHFW6ifZstyfg_eH-bfaUzV8fn2e386ykXPQZEYwhZChi6YkC8ZLzHHFTWWs5KwgWxlhCMSsrIQtDpDRCCqyFpVKbvBR0Am62uX4wrV2UqTroRvlQtzpslNO1-n_p6pVaurViEgvOSQq43AUE9znY2Ku2jqVtGt1ZN0SV2vOC8ZzghF5t0aQpxmCrfQ1G6ke_SgLUVn-CL_4-tkd_fdNvspyBww</recordid><startdate>20191211</startdate><enddate>20191211</enddate><creator>Schoborg, Todd A</creator><creator>Smith, Samantha L</creator><creator>Smith, Lauren N</creator><creator>Morris, H Douglas</creator><creator>Rusan, Nasser M</creator><general>The Company of Biologists Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7343-8474</orcidid><orcidid>https://orcid.org/0000-0003-0017-0942</orcidid><orcidid>https://orcid.org/0000-0002-4194-1072</orcidid></search><sort><creationdate>20191211</creationdate><title>Micro-computed tomography as a platform for exploring Drosophila development</title><author>Schoborg, Todd A ; Smith, Samantha L ; Smith, Lauren N ; Morris, H Douglas ; Rusan, Nasser M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-286600b306228507c77407bfeee765218bbe2316cf895b299b8981a8e39ab4c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Drosophila melanogaster</topic><topic>Drosophila Proteins - genetics</topic><topic>Drosophila Proteins - metabolism</topic><topic>Embryo, Nonmammalian - diagnostic imaging</topic><topic>Embryo, Nonmammalian - embryology</topic><topic>Humans</topic><topic>Microcephaly - diagnostic imaging</topic><topic>Microcephaly - embryology</topic><topic>Microcephaly - genetics</topic><topic>Mutation</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Techniques and Resources</topic><topic>X-Ray Microtomography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schoborg, Todd A</creatorcontrib><creatorcontrib>Smith, Samantha L</creatorcontrib><creatorcontrib>Smith, Lauren N</creatorcontrib><creatorcontrib>Morris, H Douglas</creatorcontrib><creatorcontrib>Rusan, Nasser M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Development (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schoborg, Todd A</au><au>Smith, Samantha L</au><au>Smith, Lauren N</au><au>Morris, H Douglas</au><au>Rusan, Nasser M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Micro-computed tomography as a platform for exploring Drosophila development</atitle><jtitle>Development (Cambridge)</jtitle><addtitle>Development</addtitle><date>2019-12-11</date><risdate>2019</risdate><volume>146</volume><issue>23</issue><issn>0950-1991</issn><eissn>1477-9129</eissn><abstract>Understanding how events at the molecular and cellular scales contribute to tissue form and function is key to uncovering the mechanisms driving animal development, physiology and disease. Elucidating these mechanisms has been enhanced through the study of model organisms and the use of sophisticated genetic, biochemical and imaging tools. Here, we present an accessible method for non-invasive imaging of
at high resolution using micro-computed tomography (µ-CT). We show how rapid processing of intact animals, at any developmental stage, provides precise quantitative assessment of tissue size and morphology, and permits analysis of inter-organ relationships. We then use µ-CT imaging to study growth defects in the
brain through the characterization of
(
) and
(
), orthologs of the two most commonly mutated genes in human microcephaly patients. Our work demonstrates the power of combining µ-CT with traditional genetic, cellular and developmental biology tools available in model organisms to address novel biological mechanisms that control animal development and disease.</abstract><cop>England</cop><pub>The Company of Biologists Ltd</pub><pmid>31722883</pmid><doi>10.1242/dev.176685</doi><orcidid>https://orcid.org/0000-0001-7343-8474</orcidid><orcidid>https://orcid.org/0000-0003-0017-0942</orcidid><orcidid>https://orcid.org/0000-0002-4194-1072</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0950-1991 |
| ispartof | Development (Cambridge), 2019-12, Vol.146 (23) |
| issn | 0950-1991 1477-9129 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6918772 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Company of Biologists |
| subjects | Animals Drosophila melanogaster Drosophila Proteins - genetics Drosophila Proteins - metabolism Embryo, Nonmammalian - diagnostic imaging Embryo, Nonmammalian - embryology Humans Microcephaly - diagnostic imaging Microcephaly - embryology Microcephaly - genetics Mutation Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Techniques and Resources X-Ray Microtomography |
| title | Micro-computed tomography as a platform for exploring Drosophila development |
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