A Human Umbilical Cord Mesenchymal Stem Cell-Conditioned Medium/Chitosan/Collagen/β-Glycerophosphate Thermosensitive Hydrogel Promotes Burn Injury Healing in Mice
We investigated the effects of a human umbilical cord mesenchymal stem cell-conditioned medium (MSC-CM)/chitosan/collagen/β-glycerophosphate (β-GP) thermosensitive hydrogel (MSC-CM/hydrogel) on mice with third-degree burns. MSC-CM was collected and mixed with chitosan, collagen, and β-GP to generate...
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| creator | Li, Dong Shi, Qing Zhang, Bing Li, Xue Zhou, Panpan Ju, Xiuli |
| description | We investigated the effects of a human umbilical cord mesenchymal stem cell-conditioned medium (MSC-CM)/chitosan/collagen/β-glycerophosphate (β-GP) thermosensitive hydrogel (MSC-CM/hydrogel) on mice with third-degree burns. MSC-CM was collected and mixed with chitosan, collagen, and β-GP to generate the thermosensitive MSC-CM/hydrogel, which was stored in the liquid phase at 4°C. The wounds of established third-degree burned mice were then externally covered with the MSC-CM/hydrogel, which formed a gel when placed on the wounds at physiological temperature. Injured mice in three additional groups were treated with unconditioned MSC medium (UM), MSC-CM, or UM/chitosan/collagen/β-GP thermosensitive hydrogels. Skin wound samples were obtained 4, 14, and 28 days after burning for further analysis by hematoxylin and eosin and Ki-67 staining. Wound healing rates and times, in addition to immunohistochemical results, were then compared and analyzed among the four groups. Application of the MSC-CM/hydrogel shortened healing time, limited the area of inflammation, enhanced reepithelialization, promoted the formation of high-quality, well-vascularized granulation tissue, and attenuated the formation of fibrotic and hypertrophic scar tissue. In summary, MSC-CM/hydrogel effectively promotes wound healing in third-degree burned mice. |
| doi_str_mv | 10.1155/2019/5768285 |
| format | Article |
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MSC-CM was collected and mixed with chitosan, collagen, and β-GP to generate the thermosensitive MSC-CM/hydrogel, which was stored in the liquid phase at 4°C. The wounds of established third-degree burned mice were then externally covered with the MSC-CM/hydrogel, which formed a gel when placed on the wounds at physiological temperature. Injured mice in three additional groups were treated with unconditioned MSC medium (UM), MSC-CM, or UM/chitosan/collagen/β-GP thermosensitive hydrogels. Skin wound samples were obtained 4, 14, and 28 days after burning for further analysis by hematoxylin and eosin and Ki-67 staining. Wound healing rates and times, in addition to immunohistochemical results, were then compared and analyzed among the four groups. Application of the MSC-CM/hydrogel shortened healing time, limited the area of inflammation, enhanced reepithelialization, promoted the formation of high-quality, well-vascularized granulation tissue, and attenuated the formation of fibrotic and hypertrophic scar tissue. In summary, MSC-CM/hydrogel effectively promotes wound healing in third-degree burned mice.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2019/5768285</identifier><identifier>PMID: 31886229</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Animals ; Biocompatibility ; Burning ; Burns ; Burns - pathology ; Cell adhesion & migration ; Cells, Cultured ; Chitosan ; Chitosan - chemistry ; Collagen ; Collagen - chemistry ; Culture Media, Conditioned - chemistry ; Culture Media, Conditioned - pharmacology ; Cytotoxicity ; Eutrophication ; FDA approval ; Fibrosis ; Flow cytometry ; Glycerophosphate ; Glycerophosphates - chemistry ; Granulation ; Humans ; Hydrogels ; Hydrogels - chemistry ; Hydrogels - pharmacology ; Liquid phases ; Male ; Medical prognosis ; Mesenchymal Stem Cells - cytology ; Mesenchymal Stem Cells - metabolism ; Mesenchyme ; Mice ; Mice, Inbred C57BL ; Morphology ; Physiology ; Stem cells ; Temperature ; Umbilical cord ; Umbilical Cord - cytology ; Umbilical Cord - metabolism ; Viscosity ; Wound healing ; Wound Healing - drug effects ; β-Glycerophosphoric acid</subject><ispartof>BioMed research international, 2019, Vol.2019 (2019), p.1-14</ispartof><rights>Copyright © 2019 Panpan Zhou et al.</rights><rights>Copyright © 2019 Panpan Zhou et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. http://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2019 Panpan Zhou et al. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-b446d3aad7010ac00f88bf3b38d98303c2507ee09b6e2ab5629341fb5f984af93</citedby><cites>FETCH-LOGICAL-c471t-b446d3aad7010ac00f88bf3b38d98303c2507ee09b6e2ab5629341fb5f984af93</cites><orcidid>0000-0001-5464-3106 ; 0000-0002-1019-6436 ; 0000-0002-6496-4605</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915016/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915016/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31886229$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ono, Shoichiro</contributor><creatorcontrib>Li, Dong</creatorcontrib><creatorcontrib>Shi, Qing</creatorcontrib><creatorcontrib>Zhang, Bing</creatorcontrib><creatorcontrib>Li, Xue</creatorcontrib><creatorcontrib>Zhou, Panpan</creatorcontrib><creatorcontrib>Ju, Xiuli</creatorcontrib><title>A Human Umbilical Cord Mesenchymal Stem Cell-Conditioned Medium/Chitosan/Collagen/β-Glycerophosphate Thermosensitive Hydrogel Promotes Burn Injury Healing in Mice</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>We investigated the effects of a human umbilical cord mesenchymal stem cell-conditioned medium (MSC-CM)/chitosan/collagen/β-glycerophosphate (β-GP) thermosensitive hydrogel (MSC-CM/hydrogel) on mice with third-degree burns. MSC-CM was collected and mixed with chitosan, collagen, and β-GP to generate the thermosensitive MSC-CM/hydrogel, which was stored in the liquid phase at 4°C. The wounds of established third-degree burned mice were then externally covered with the MSC-CM/hydrogel, which formed a gel when placed on the wounds at physiological temperature. Injured mice in three additional groups were treated with unconditioned MSC medium (UM), MSC-CM, or UM/chitosan/collagen/β-GP thermosensitive hydrogels. Skin wound samples were obtained 4, 14, and 28 days after burning for further analysis by hematoxylin and eosin and Ki-67 staining. Wound healing rates and times, in addition to immunohistochemical results, were then compared and analyzed among the four groups. Application of the MSC-CM/hydrogel shortened healing time, limited the area of inflammation, enhanced reepithelialization, promoted the formation of high-quality, well-vascularized granulation tissue, and attenuated the formation of fibrotic and hypertrophic scar tissue. In summary, MSC-CM/hydrogel effectively promotes wound healing in third-degree burned mice.</description><subject>Animals</subject><subject>Biocompatibility</subject><subject>Burning</subject><subject>Burns</subject><subject>Burns - pathology</subject><subject>Cell adhesion & migration</subject><subject>Cells, Cultured</subject><subject>Chitosan</subject><subject>Chitosan - chemistry</subject><subject>Collagen</subject><subject>Collagen - chemistry</subject><subject>Culture Media, Conditioned - chemistry</subject><subject>Culture Media, Conditioned - pharmacology</subject><subject>Cytotoxicity</subject><subject>Eutrophication</subject><subject>FDA approval</subject><subject>Fibrosis</subject><subject>Flow cytometry</subject><subject>Glycerophosphate</subject><subject>Glycerophosphates - chemistry</subject><subject>Granulation</subject><subject>Humans</subject><subject>Hydrogels</subject><subject>Hydrogels - chemistry</subject><subject>Hydrogels - pharmacology</subject><subject>Liquid phases</subject><subject>Male</subject><subject>Medical prognosis</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>Mesenchymal Stem Cells - metabolism</subject><subject>Mesenchyme</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Morphology</subject><subject>Physiology</subject><subject>Stem cells</subject><subject>Temperature</subject><subject>Umbilical cord</subject><subject>Umbilical Cord - cytology</subject><subject>Umbilical Cord - metabolism</subject><subject>Viscosity</subject><subject>Wound healing</subject><subject>Wound Healing - drug effects</subject><subject>β-Glycerophosphoric acid</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkstu1DAUhiMEolXpjjWyxAYJQnxJHGeD1EbQqdQKJNq15SQnE48ce7CTojwPb8CD8Ex4NMNwWeHNsezv_DqXP0meE_yWkKLIKCZVVpRcUFE8Sk4pI3nKSU4eH--MnSTnIWxwPIJwXPGnyQkjQnBKq9Pk2wVazaOy6H5stNGtMqh2vkO3EMC2wzLGh88TjKgGY9La2U5P2lnYEZ2ex6we9OSCslntjFFrsNmP7-mVWVrwbju4sB3UBOhuAD-6KBli-gOg1dJ5twaDPnk3ugkCupy9Rdd2M_sFrUAZbddIW3SrW3iWPOmVCXB-iGfJ_Yf3d_Uqvfl4dV1f3KRtXpIpbfKcd0yprsQEqxbjXoimZw0TXSUYZi0tcAmAq4YDVU3BacVy0jdFX4lc9RU7S97tdbdzM0LXgp28MnLr9aj8Ip3S8u8fqwe5dg-SV6TAhEeBVwcB777MECY56tDGwSkLbg6SsrgTWghMI_ryH3Tj4gRie5GipaB5QUWk3uyp1rsQPPTHYgiWOwPInQHkwQARf_FnA0f417oj8HoPDNp26qv-TzmIDPTqN00opzlnPwHNeMWB</recordid><startdate>2019</startdate><enddate>2019</enddate><creator>Li, Dong</creator><creator>Shi, Qing</creator><creator>Zhang, Bing</creator><creator>Li, Xue</creator><creator>Zhou, Panpan</creator><creator>Ju, Xiuli</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5464-3106</orcidid><orcidid>https://orcid.org/0000-0002-1019-6436</orcidid><orcidid>https://orcid.org/0000-0002-6496-4605</orcidid></search><sort><creationdate>2019</creationdate><title>A Human Umbilical Cord Mesenchymal Stem Cell-Conditioned Medium/Chitosan/Collagen/β-Glycerophosphate Thermosensitive Hydrogel Promotes Burn Injury Healing in Mice</title><author>Li, Dong ; Shi, Qing ; Zhang, Bing ; Li, Xue ; Zhou, Panpan ; Ju, Xiuli</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-b446d3aad7010ac00f88bf3b38d98303c2507ee09b6e2ab5629341fb5f984af93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Biocompatibility</topic><topic>Burning</topic><topic>Burns</topic><topic>Burns - pathology</topic><topic>Cell adhesion & migration</topic><topic>Cells, Cultured</topic><topic>Chitosan</topic><topic>Chitosan - chemistry</topic><topic>Collagen</topic><topic>Collagen - chemistry</topic><topic>Culture Media, Conditioned - chemistry</topic><topic>Culture Media, Conditioned - pharmacology</topic><topic>Cytotoxicity</topic><topic>Eutrophication</topic><topic>FDA approval</topic><topic>Fibrosis</topic><topic>Flow cytometry</topic><topic>Glycerophosphate</topic><topic>Glycerophosphates - chemistry</topic><topic>Granulation</topic><topic>Humans</topic><topic>Hydrogels</topic><topic>Hydrogels - chemistry</topic><topic>Hydrogels - pharmacology</topic><topic>Liquid phases</topic><topic>Male</topic><topic>Medical prognosis</topic><topic>Mesenchymal Stem Cells - cytology</topic><topic>Mesenchymal Stem Cells - metabolism</topic><topic>Mesenchyme</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Morphology</topic><topic>Physiology</topic><topic>Stem cells</topic><topic>Temperature</topic><topic>Umbilical cord</topic><topic>Umbilical Cord - cytology</topic><topic>Umbilical Cord - metabolism</topic><topic>Viscosity</topic><topic>Wound healing</topic><topic>Wound Healing - drug effects</topic><topic>β-Glycerophosphoric acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Dong</creatorcontrib><creatorcontrib>Shi, Qing</creatorcontrib><creatorcontrib>Zhang, Bing</creatorcontrib><creatorcontrib>Li, Xue</creatorcontrib><creatorcontrib>Zhou, Panpan</creatorcontrib><creatorcontrib>Ju, Xiuli</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Dong</au><au>Shi, Qing</au><au>Zhang, Bing</au><au>Li, Xue</au><au>Zhou, Panpan</au><au>Ju, Xiuli</au><au>Ono, Shoichiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Human Umbilical Cord Mesenchymal Stem Cell-Conditioned Medium/Chitosan/Collagen/β-Glycerophosphate Thermosensitive Hydrogel Promotes Burn Injury Healing in Mice</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2019</date><risdate>2019</risdate><volume>2019</volume><issue>2019</issue><spage>1</spage><epage>14</epage><pages>1-14</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>We investigated the effects of a human umbilical cord mesenchymal stem cell-conditioned medium (MSC-CM)/chitosan/collagen/β-glycerophosphate (β-GP) thermosensitive hydrogel (MSC-CM/hydrogel) on mice with third-degree burns. MSC-CM was collected and mixed with chitosan, collagen, and β-GP to generate the thermosensitive MSC-CM/hydrogel, which was stored in the liquid phase at 4°C. The wounds of established third-degree burned mice were then externally covered with the MSC-CM/hydrogel, which formed a gel when placed on the wounds at physiological temperature. Injured mice in three additional groups were treated with unconditioned MSC medium (UM), MSC-CM, or UM/chitosan/collagen/β-GP thermosensitive hydrogels. Skin wound samples were obtained 4, 14, and 28 days after burning for further analysis by hematoxylin and eosin and Ki-67 staining. Wound healing rates and times, in addition to immunohistochemical results, were then compared and analyzed among the four groups. Application of the MSC-CM/hydrogel shortened healing time, limited the area of inflammation, enhanced reepithelialization, promoted the formation of high-quality, well-vascularized granulation tissue, and attenuated the formation of fibrotic and hypertrophic scar tissue. In summary, MSC-CM/hydrogel effectively promotes wound healing in third-degree burned mice.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>31886229</pmid><doi>10.1155/2019/5768285</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0001-5464-3106</orcidid><orcidid>https://orcid.org/0000-0002-1019-6436</orcidid><orcidid>https://orcid.org/0000-0002-6496-4605</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biocompatibility Burning Burns Burns - pathology Cell adhesion & migration Cells, Cultured Chitosan Chitosan - chemistry Collagen Collagen - chemistry Culture Media, Conditioned - chemistry Culture Media, Conditioned - pharmacology Cytotoxicity Eutrophication FDA approval Fibrosis Flow cytometry Glycerophosphate Glycerophosphates - chemistry Granulation Humans Hydrogels Hydrogels - chemistry Hydrogels - pharmacology Liquid phases Male Medical prognosis Mesenchymal Stem Cells - cytology Mesenchymal Stem Cells - metabolism Mesenchyme Mice Mice, Inbred C57BL Morphology Physiology Stem cells Temperature Umbilical cord Umbilical Cord - cytology Umbilical Cord - metabolism Viscosity Wound healing Wound Healing - drug effects β-Glycerophosphoric acid |
| title | A Human Umbilical Cord Mesenchymal Stem Cell-Conditioned Medium/Chitosan/Collagen/β-Glycerophosphate Thermosensitive Hydrogel Promotes Burn Injury Healing in Mice |
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