Architecture and modular assembly of Sulfolobus S-layers revealed by electron cryotomography
Surface protein layers (S-layers) often form the only structural component of the archaeal cell wall and are therefore important for cell survival. S-layers have a plethora of cellular functions including maintenance of cell shape, osmotic, and mechanical stability, the formation of a semipermeable...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2019-12, Vol.116 (50), p.25278-25286 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Gambelli, Lavinia Meyer, Benjamin H. McLaren, Mathew Sanders, Kelly Quax, Tessa E. F. Gold, Vicki A. M. Albers, Sonja-Verena Daum, Bertram |
| description | Surface protein layers (S-layers) often form the only structural component of the archaeal cell wall and are therefore important for cell survival. S-layers have a plethora of cellular functions including maintenance of cell shape, osmotic, and mechanical stability, the formation of a semipermeable protective barrier around the cell, and cell–cell interaction, as well as surface adhesion. Despite the central importance of S-layers for archaeal life, their 3-dimensional (3D) architecture is still poorly understood. Here we present detailed 3D electron cryomicroscopy maps of archaeal S-layers from 3 different Sulfolobus strains. We were able to pinpoint the positions and determine the structure of the 2 subunits SlaA and SlaB. We also present a model describing the assembly of the mature S-layer. |
| doi_str_mv | 10.1073/pnas.1911262116 |
| format | Article |
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F. ; Gold, Vicki A. M. ; Albers, Sonja-Verena ; Daum, Bertram</creator><creatorcontrib>Gambelli, Lavinia ; Meyer, Benjamin H. ; McLaren, Mathew ; Sanders, Kelly ; Quax, Tessa E. F. ; Gold, Vicki A. M. ; Albers, Sonja-Verena ; Daum, Bertram</creatorcontrib><description>Surface protein layers (S-layers) often form the only structural component of the archaeal cell wall and are therefore important for cell survival. S-layers have a plethora of cellular functions including maintenance of cell shape, osmotic, and mechanical stability, the formation of a semipermeable protective barrier around the cell, and cell–cell interaction, as well as surface adhesion. Despite the central importance of S-layers for archaeal life, their 3-dimensional (3D) architecture is still poorly understood. Here we present detailed 3D electron cryomicroscopy maps of archaeal S-layers from 3 different Sulfolobus strains. We were able to pinpoint the positions and determine the structure of the 2 subunits SlaA and SlaB. We also present a model describing the assembly of the mature S-layer.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.1911262116</identifier><identifier>PMID: 31767763</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Architecture ; Assembly ; Biological Sciences ; Cell size ; Cell survival ; Cell walls ; Cryoelectron Microscopy ; Dimerization ; Maintenance ; Membrane Glycoproteins - chemistry ; Membrane Glycoproteins - genetics ; Membrane Glycoproteins - metabolism ; Membrane Glycoproteins - ultrastructure ; Sulfolobus ; Sulfolobus - chemistry ; Sulfolobus - genetics ; Sulfolobus - metabolism ; Sulfolobus - ultrastructure</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2019-12, Vol.116 (50), p.25278-25286</ispartof><rights>Copyright © 2019 the Author(s). Published by PNAS.</rights><rights>Copyright National Academy of Sciences Dec 10, 2019</rights><rights>Copyright © 2019 the Author(s). 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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 2019-12, Vol.116 (50), p.25278-25286 |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Architecture Assembly Biological Sciences Cell size Cell survival Cell walls Cryoelectron Microscopy Dimerization Maintenance Membrane Glycoproteins - chemistry Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism Membrane Glycoproteins - ultrastructure Sulfolobus Sulfolobus - chemistry Sulfolobus - genetics Sulfolobus - metabolism Sulfolobus - ultrastructure |
| title | Architecture and modular assembly of Sulfolobus S-layers revealed by electron cryotomography |
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