miR-190b promotes colorectal cancer progression through targeting forkhead box protein P2
microRNA-190b (miR-190b) is abnormally expressed in multiple types of cancer, however, its role in colorectal cancer (CRC) is largely unknown. In the present study, it was demonstrated that miR-190b expression was upregulated in CRC cell lines compared with the normal epithelial colon cell line. Kno...
Gespeichert in:
| Veröffentlicht in: | Experimental and therapeutic medicine 2020-01, Vol.19 (1), p.79-84 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 84 |
|---|---|
| container_issue | 1 |
| container_start_page | 79 |
| container_title | Experimental and therapeutic medicine |
| container_volume | 19 |
| creator | Zhao, Qiang Liu, Chengkui Cui, Qing Luan, Xuerong Wang, Qingfeng Zhou, Chengfu |
| description | microRNA-190b (miR-190b) is abnormally expressed in multiple types of cancer, however, its role in colorectal cancer (CRC) is largely unknown. In the present study, it was demonstrated that miR-190b expression was upregulated in CRC cell lines compared with the normal epithelial colon cell line. Knockdown of miR-190b decreased proliferation, colony formation and invasion, and increased apoptosis of CRC cells. Furthermore, forkhead box protein P2 (FOXP2) was predicted as a target of miR-190b and further validated by luciferase activity reporter assay and western blotting. Rescue experiments showed that knockdown of FOXP2 reversed the inhibitory effects of miR-190b inhibitor on the behavior of the CRC cell lines. Taken together, the present study demonstrated the oncogenic role of miR-190b in CRC through regulation of FOXP2 expression. |
| doi_str_mv | 10.3892/etm.2019.8175 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6909528</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A615363346</galeid><sourcerecordid>A615363346</sourcerecordid><originalsourceid>FETCH-LOGICAL-c412t-effb65e18428f9bddf65df4f9e2d304e9bb30911ac1bffc90d81d7c291972c0b3</originalsourceid><addsrcrecordid>eNptks-L1TAQx4so7rLu0asUvHjpM5M0bXIRlmX9AQuK6MFTSNNJX9Y2eSap6H9vyj5XV0wOGTKf-YbJfKvqKZAdE5K-xLzsKAG5E9DzB9Up9JI2QIA_PMZECjipzlO6IWXxDoTgj6sTBoIz2vPT6sviPjYgyVAfYlhCxlSbMIeIJuu5NtobjFtqipiSC77O-xjWaV9nHSfMzk-1DfHrHvVYD-HHhmZ0vv5An1SPrJ4Tnh_Ps-rz66tPl2-b6_dv3l1eXDemBZobtHboOIJoqbByGEfb8dG2ViIdGWlRDgMjEkAbGKw1kowCxt5QCbKnhgzsrHp1q3tYhwVHgz5HPatDdIuOP1XQTt3PeLdXU_iuOkkkp6IIvDgKxPBtxZTV4pLBedYew5oUZVT0vJNdV9Dn_6A3YY2-tFcoVkbS9wL-UJOeUTlvQ3nXbKLqogPOOsbaTWv3H6rsERdngkfryv29gua2wMSQUkR71yMQtdlBFTuozQ5qs0Phn_39MXf07-GzX84SsJU</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2333897781</pqid></control><display><type>article</type><title>miR-190b promotes colorectal cancer progression through targeting forkhead box protein P2</title><source>PubMed Central</source><creator>Zhao, Qiang ; Liu, Chengkui ; Cui, Qing ; Luan, Xuerong ; Wang, Qingfeng ; Zhou, Chengfu</creator><creatorcontrib>Zhao, Qiang ; Liu, Chengkui ; Cui, Qing ; Luan, Xuerong ; Wang, Qingfeng ; Zhou, Chengfu</creatorcontrib><description>microRNA-190b (miR-190b) is abnormally expressed in multiple types of cancer, however, its role in colorectal cancer (CRC) is largely unknown. In the present study, it was demonstrated that miR-190b expression was upregulated in CRC cell lines compared with the normal epithelial colon cell line. Knockdown of miR-190b decreased proliferation, colony formation and invasion, and increased apoptosis of CRC cells. Furthermore, forkhead box protein P2 (FOXP2) was predicted as a target of miR-190b and further validated by luciferase activity reporter assay and western blotting. Rescue experiments showed that knockdown of FOXP2 reversed the inhibitory effects of miR-190b inhibitor on the behavior of the CRC cell lines. Taken together, the present study demonstrated the oncogenic role of miR-190b in CRC through regulation of FOXP2 expression.</description><identifier>ISSN: 1792-0981</identifier><identifier>EISSN: 1792-1015</identifier><identifier>DOI: 10.3892/etm.2019.8175</identifier><identifier>PMID: 31853275</identifier><language>eng</language><publisher>Greece: Spandidos Publications</publisher><subject>Apoptosis ; Biotechnology ; Bone cancer ; Cell growth ; Colorectal cancer ; Comparative analysis ; Experiments ; Gastric cancer ; Gene expression ; Luciferase ; MicroRNA ; Proteins ; Scientific equipment industry ; Statistical analysis</subject><ispartof>Experimental and therapeutic medicine, 2020-01, Vol.19 (1), p.79-84</ispartof><rights>Copyright: © Zhao et al.</rights><rights>COPYRIGHT 2020 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2020</rights><rights>Copyright: © Zhao et al. 2019</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-effb65e18428f9bddf65df4f9e2d304e9bb30911ac1bffc90d81d7c291972c0b3</citedby><cites>FETCH-LOGICAL-c412t-effb65e18428f9bddf65df4f9e2d304e9bb30911ac1bffc90d81d7c291972c0b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6909528/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6909528/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31853275$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Qiang</creatorcontrib><creatorcontrib>Liu, Chengkui</creatorcontrib><creatorcontrib>Cui, Qing</creatorcontrib><creatorcontrib>Luan, Xuerong</creatorcontrib><creatorcontrib>Wang, Qingfeng</creatorcontrib><creatorcontrib>Zhou, Chengfu</creatorcontrib><title>miR-190b promotes colorectal cancer progression through targeting forkhead box protein P2</title><title>Experimental and therapeutic medicine</title><addtitle>Exp Ther Med</addtitle><description>microRNA-190b (miR-190b) is abnormally expressed in multiple types of cancer, however, its role in colorectal cancer (CRC) is largely unknown. In the present study, it was demonstrated that miR-190b expression was upregulated in CRC cell lines compared with the normal epithelial colon cell line. Knockdown of miR-190b decreased proliferation, colony formation and invasion, and increased apoptosis of CRC cells. Furthermore, forkhead box protein P2 (FOXP2) was predicted as a target of miR-190b and further validated by luciferase activity reporter assay and western blotting. Rescue experiments showed that knockdown of FOXP2 reversed the inhibitory effects of miR-190b inhibitor on the behavior of the CRC cell lines. Taken together, the present study demonstrated the oncogenic role of miR-190b in CRC through regulation of FOXP2 expression.</description><subject>Apoptosis</subject><subject>Biotechnology</subject><subject>Bone cancer</subject><subject>Cell growth</subject><subject>Colorectal cancer</subject><subject>Comparative analysis</subject><subject>Experiments</subject><subject>Gastric cancer</subject><subject>Gene expression</subject><subject>Luciferase</subject><subject>MicroRNA</subject><subject>Proteins</subject><subject>Scientific equipment industry</subject><subject>Statistical analysis</subject><issn>1792-0981</issn><issn>1792-1015</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNptks-L1TAQx4so7rLu0asUvHjpM5M0bXIRlmX9AQuK6MFTSNNJX9Y2eSap6H9vyj5XV0wOGTKf-YbJfKvqKZAdE5K-xLzsKAG5E9DzB9Up9JI2QIA_PMZECjipzlO6IWXxDoTgj6sTBoIz2vPT6sviPjYgyVAfYlhCxlSbMIeIJuu5NtobjFtqipiSC77O-xjWaV9nHSfMzk-1DfHrHvVYD-HHhmZ0vv5An1SPrJ4Tnh_Ps-rz66tPl2-b6_dv3l1eXDemBZobtHboOIJoqbByGEfb8dG2ViIdGWlRDgMjEkAbGKw1kowCxt5QCbKnhgzsrHp1q3tYhwVHgz5HPatDdIuOP1XQTt3PeLdXU_iuOkkkp6IIvDgKxPBtxZTV4pLBedYew5oUZVT0vJNdV9Dn_6A3YY2-tFcoVkbS9wL-UJOeUTlvQ3nXbKLqogPOOsbaTWv3H6rsERdngkfryv29gua2wMSQUkR71yMQtdlBFTuozQ5qs0Phn_39MXf07-GzX84SsJU</recordid><startdate>20200101</startdate><enddate>20200101</enddate><creator>Zhao, Qiang</creator><creator>Liu, Chengkui</creator><creator>Cui, Qing</creator><creator>Luan, Xuerong</creator><creator>Wang, Qingfeng</creator><creator>Zhou, Chengfu</creator><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><general>D.A. Spandidos</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200101</creationdate><title>miR-190b promotes colorectal cancer progression through targeting forkhead box protein P2</title><author>Zhao, Qiang ; Liu, Chengkui ; Cui, Qing ; Luan, Xuerong ; Wang, Qingfeng ; Zhou, Chengfu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-effb65e18428f9bddf65df4f9e2d304e9bb30911ac1bffc90d81d7c291972c0b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Apoptosis</topic><topic>Biotechnology</topic><topic>Bone cancer</topic><topic>Cell growth</topic><topic>Colorectal cancer</topic><topic>Comparative analysis</topic><topic>Experiments</topic><topic>Gastric cancer</topic><topic>Gene expression</topic><topic>Luciferase</topic><topic>MicroRNA</topic><topic>Proteins</topic><topic>Scientific equipment industry</topic><topic>Statistical analysis</topic><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Qiang</creatorcontrib><creatorcontrib>Liu, Chengkui</creatorcontrib><creatorcontrib>Cui, Qing</creatorcontrib><creatorcontrib>Luan, Xuerong</creatorcontrib><creatorcontrib>Wang, Qingfeng</creatorcontrib><creatorcontrib>Zhou, Chengfu</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental and therapeutic medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Qiang</au><au>Liu, Chengkui</au><au>Cui, Qing</au><au>Luan, Xuerong</au><au>Wang, Qingfeng</au><au>Zhou, Chengfu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>miR-190b promotes colorectal cancer progression through targeting forkhead box protein P2</atitle><jtitle>Experimental and therapeutic medicine</jtitle><addtitle>Exp Ther Med</addtitle><date>2020-01-01</date><risdate>2020</risdate><volume>19</volume><issue>1</issue><spage>79</spage><epage>84</epage><pages>79-84</pages><issn>1792-0981</issn><eissn>1792-1015</eissn><abstract>microRNA-190b (miR-190b) is abnormally expressed in multiple types of cancer, however, its role in colorectal cancer (CRC) is largely unknown. In the present study, it was demonstrated that miR-190b expression was upregulated in CRC cell lines compared with the normal epithelial colon cell line. Knockdown of miR-190b decreased proliferation, colony formation and invasion, and increased apoptosis of CRC cells. Furthermore, forkhead box protein P2 (FOXP2) was predicted as a target of miR-190b and further validated by luciferase activity reporter assay and western blotting. Rescue experiments showed that knockdown of FOXP2 reversed the inhibitory effects of miR-190b inhibitor on the behavior of the CRC cell lines. Taken together, the present study demonstrated the oncogenic role of miR-190b in CRC through regulation of FOXP2 expression.</abstract><cop>Greece</cop><pub>Spandidos Publications</pub><pmid>31853275</pmid><doi>10.3892/etm.2019.8175</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1792-0981 |
| ispartof | Experimental and therapeutic medicine, 2020-01, Vol.19 (1), p.79-84 |
| issn | 1792-0981 1792-1015 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6909528 |
| source | PubMed Central |
| subjects | Apoptosis Biotechnology Bone cancer Cell growth Colorectal cancer Comparative analysis Experiments Gastric cancer Gene expression Luciferase MicroRNA Proteins Scientific equipment industry Statistical analysis |
| title | miR-190b promotes colorectal cancer progression through targeting forkhead box protein P2 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T08%3A26%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=miR-190b%20promotes%20colorectal%20cancer%20progression%20through%20targeting%20forkhead%20box%20protein%20P2&rft.jtitle=Experimental%20and%20therapeutic%20medicine&rft.au=Zhao,%20Qiang&rft.date=2020-01-01&rft.volume=19&rft.issue=1&rft.spage=79&rft.epage=84&rft.pages=79-84&rft.issn=1792-0981&rft.eissn=1792-1015&rft_id=info:doi/10.3892/etm.2019.8175&rft_dat=%3Cgale_pubme%3EA615363346%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2333897781&rft_id=info:pmid/31853275&rft_galeid=A615363346&rfr_iscdi=true |