Discovery of a bone‐like blood particle in the peripheral circulation of humans and rodents
Objective To characterize ossified bone marrow blood vessels and confirm the presence of ossified particles (OSP) in humans and rodents. Methods Human bone marrow blood vessels were processed for scanning and transmission electron microscopy. Whole blood samples were collected from younger (26‐39 ye...
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| Veröffentlicht in: | Microcirculation (New York, N.Y. 1994) N.Y. 1994), 2019-11, Vol.26 (8), p.e12579-n/a |
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| creator | Prisby, Rhonda Ross, Jean Opdenaker, Lynn McLane, Mary Ann Lee, Seungyong Sun, Xiangle Guderian, Sophie |
| description | Objective
To characterize ossified bone marrow blood vessels and confirm the presence of ossified particles (OSP) in humans and rodents.
Methods
Human bone marrow blood vessels were processed for scanning and transmission electron microscopy. Whole blood samples were collected from younger (26‐39 years; n = 6) and older (55‐63 years; n = 6) volunteers and male Fischer‐344 rats (1 month, n = 7; 6 months, n = 7; 12 months, n = 7; 18‐months, n = 6; 24 months, n = 8). OSP in the whole blood samples were sorted and imaged with microscopy to determine diameter, circularity, and solidity. Additionally, the chemical composition of OSP was determined via elemental analysis.
Results
SEM revealed two types of ossified bone marrow blood vessels: that is, “transitioning” and “ossified.” OSP were adhered to the surface of transitioning vessels and theoretically gain access to and circulate within the blood. The majority of OSP were ≤15 μm in diameter, but many were of sufficient size to serve as emboli (ie, >15 μm).OSP were predominately oblong in shape and several had jagged tips and edges.
Conclusions
We introduce a novel, bone‐like blood particle that may be diagnostic of bone marrow blood vessel ossification. Further, OSP may associate with several disease states (eg, atherosclerosis). |
| doi_str_mv | 10.1111/micc.12579 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6899169</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2248384921</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4079-33de2b342ea0e54863e5cbcc3b2b0186e7857820a34075ece94bbd576eddcdb43</originalsourceid><addsrcrecordid>eNp9kc1uFSEYhomxsbW68QIMiRtjMpW_mYGNSXP8aZMaN7o0hJ_veKjMMMJMzdl5CV6jVyKnp23UhWwg4eHhhRehJ5Sc0DpeDsG5E8raXt1DR7QVqpE9VffrmvS8UZ2Uh-hhKZeEECmZeoAOOWWi41wcoc-vQ3HpCvIWpzU22KYRfv34GcNXwDam5PFk8hxcBBxGPG8AT5DDtIFsInYhuyWaOaRxd3qzDGYs2Iwe5-RhnMsjdLA2scDjm_kYfXr75uPqrLn48O58dXrROEF61XDugVkuGBgCrZAdh9ZZ57hlllDZQS_bXjJieMVbcKCEtb7tO_DeeSv4MXq1906LHcC7enfNp6ccBpO3Opmg_94Zw0Z_SVe6k0rRTlXB8xtBTt8WKLMe6r9AjGaEtBTNmJBcCsVoRZ_9g16mJY_1eZpxxjhRiveVerGnXE6lZFjfhaFE71rTu9b0dWsVfvpn_Dv0tqYK0D3wPUTY_kel35-vVnvpb6qapKc</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2322309937</pqid></control><display><type>article</type><title>Discovery of a bone‐like blood particle in the peripheral circulation of humans and rodents</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Prisby, Rhonda ; Ross, Jean ; Opdenaker, Lynn ; McLane, Mary Ann ; Lee, Seungyong ; Sun, Xiangle ; Guderian, Sophie</creator><creatorcontrib>Prisby, Rhonda ; Ross, Jean ; Opdenaker, Lynn ; McLane, Mary Ann ; Lee, Seungyong ; Sun, Xiangle ; Guderian, Sophie</creatorcontrib><description>Objective
To characterize ossified bone marrow blood vessels and confirm the presence of ossified particles (OSP) in humans and rodents.
Methods
Human bone marrow blood vessels were processed for scanning and transmission electron microscopy. Whole blood samples were collected from younger (26‐39 years; n = 6) and older (55‐63 years; n = 6) volunteers and male Fischer‐344 rats (1 month, n = 7; 6 months, n = 7; 12 months, n = 7; 18‐months, n = 6; 24 months, n = 8). OSP in the whole blood samples were sorted and imaged with microscopy to determine diameter, circularity, and solidity. Additionally, the chemical composition of OSP was determined via elemental analysis.
Results
SEM revealed two types of ossified bone marrow blood vessels: that is, “transitioning” and “ossified.” OSP were adhered to the surface of transitioning vessels and theoretically gain access to and circulate within the blood. The majority of OSP were ≤15 μm in diameter, but many were of sufficient size to serve as emboli (ie, >15 μm).OSP were predominately oblong in shape and several had jagged tips and edges.
Conclusions
We introduce a novel, bone‐like blood particle that may be diagnostic of bone marrow blood vessel ossification. Further, OSP may associate with several disease states (eg, atherosclerosis).</description><identifier>ISSN: 1073-9688</identifier><identifier>EISSN: 1549-8719</identifier><identifier>DOI: 10.1111/micc.12579</identifier><identifier>PMID: 31246334</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Adult ; Aged ; Animals ; Bone marrow ; Bone Marrow - blood supply ; Bone Marrow - ultrastructure ; bone marrow blood vessel ossification ; Bone Marrow Diseases - blood ; Bone Marrow Diseases - pathology ; emboli ; Extracellular Vesicles - metabolism ; Extracellular Vesicles - ultrastructure ; Female ; human ; Humans ; Male ; Microscopy ; Middle Aged ; Ossification, Heterotopic - blood ; Ossification, Heterotopic - pathology ; ossified particles ; rat ; Rats ; Rats, Inbred F344 ; Vascular Calcification - blood ; Vascular Calcification - pathology</subject><ispartof>Microcirculation (New York, N.Y. 1994), 2019-11, Vol.26 (8), p.e12579-n/a</ispartof><rights>2019 John Wiley & Sons Ltd</rights><rights>2019 John Wiley & Sons Ltd.</rights><rights>Copyright © 2019 John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4079-33de2b342ea0e54863e5cbcc3b2b0186e7857820a34075ece94bbd576eddcdb43</cites><orcidid>0000-0002-1986-1447</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fmicc.12579$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fmicc.12579$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31246334$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Prisby, Rhonda</creatorcontrib><creatorcontrib>Ross, Jean</creatorcontrib><creatorcontrib>Opdenaker, Lynn</creatorcontrib><creatorcontrib>McLane, Mary Ann</creatorcontrib><creatorcontrib>Lee, Seungyong</creatorcontrib><creatorcontrib>Sun, Xiangle</creatorcontrib><creatorcontrib>Guderian, Sophie</creatorcontrib><title>Discovery of a bone‐like blood particle in the peripheral circulation of humans and rodents</title><title>Microcirculation (New York, N.Y. 1994)</title><addtitle>Microcirculation</addtitle><description>Objective
To characterize ossified bone marrow blood vessels and confirm the presence of ossified particles (OSP) in humans and rodents.
Methods
Human bone marrow blood vessels were processed for scanning and transmission electron microscopy. Whole blood samples were collected from younger (26‐39 years; n = 6) and older (55‐63 years; n = 6) volunteers and male Fischer‐344 rats (1 month, n = 7; 6 months, n = 7; 12 months, n = 7; 18‐months, n = 6; 24 months, n = 8). OSP in the whole blood samples were sorted and imaged with microscopy to determine diameter, circularity, and solidity. Additionally, the chemical composition of OSP was determined via elemental analysis.
Results
SEM revealed two types of ossified bone marrow blood vessels: that is, “transitioning” and “ossified.” OSP were adhered to the surface of transitioning vessels and theoretically gain access to and circulate within the blood. The majority of OSP were ≤15 μm in diameter, but many were of sufficient size to serve as emboli (ie, >15 μm).OSP were predominately oblong in shape and several had jagged tips and edges.
Conclusions
We introduce a novel, bone‐like blood particle that may be diagnostic of bone marrow blood vessel ossification. Further, OSP may associate with several disease states (eg, atherosclerosis).</description><subject>Adult</subject><subject>Aged</subject><subject>Animals</subject><subject>Bone marrow</subject><subject>Bone Marrow - blood supply</subject><subject>Bone Marrow - ultrastructure</subject><subject>bone marrow blood vessel ossification</subject><subject>Bone Marrow Diseases - blood</subject><subject>Bone Marrow Diseases - pathology</subject><subject>emboli</subject><subject>Extracellular Vesicles - metabolism</subject><subject>Extracellular Vesicles - ultrastructure</subject><subject>Female</subject><subject>human</subject><subject>Humans</subject><subject>Male</subject><subject>Microscopy</subject><subject>Middle Aged</subject><subject>Ossification, Heterotopic - blood</subject><subject>Ossification, Heterotopic - pathology</subject><subject>ossified particles</subject><subject>rat</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>Vascular Calcification - blood</subject><subject>Vascular Calcification - pathology</subject><issn>1073-9688</issn><issn>1549-8719</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1uFSEYhomxsbW68QIMiRtjMpW_mYGNSXP8aZMaN7o0hJ_veKjMMMJMzdl5CV6jVyKnp23UhWwg4eHhhRehJ5Sc0DpeDsG5E8raXt1DR7QVqpE9VffrmvS8UZ2Uh-hhKZeEECmZeoAOOWWi41wcoc-vQ3HpCvIWpzU22KYRfv34GcNXwDam5PFk8hxcBBxGPG8AT5DDtIFsInYhuyWaOaRxd3qzDGYs2Iwe5-RhnMsjdLA2scDjm_kYfXr75uPqrLn48O58dXrROEF61XDugVkuGBgCrZAdh9ZZ57hlllDZQS_bXjJieMVbcKCEtb7tO_DeeSv4MXq1906LHcC7enfNp6ccBpO3Opmg_94Zw0Z_SVe6k0rRTlXB8xtBTt8WKLMe6r9AjGaEtBTNmJBcCsVoRZ_9g16mJY_1eZpxxjhRiveVerGnXE6lZFjfhaFE71rTu9b0dWsVfvpn_Dv0tqYK0D3wPUTY_kel35-vVnvpb6qapKc</recordid><startdate>201911</startdate><enddate>201911</enddate><creator>Prisby, Rhonda</creator><creator>Ross, Jean</creator><creator>Opdenaker, Lynn</creator><creator>McLane, Mary Ann</creator><creator>Lee, Seungyong</creator><creator>Sun, Xiangle</creator><creator>Guderian, Sophie</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-1986-1447</orcidid></search><sort><creationdate>201911</creationdate><title>Discovery of a bone‐like blood particle in the peripheral circulation of humans and rodents</title><author>Prisby, Rhonda ; Ross, Jean ; Opdenaker, Lynn ; McLane, Mary Ann ; Lee, Seungyong ; Sun, Xiangle ; Guderian, Sophie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4079-33de2b342ea0e54863e5cbcc3b2b0186e7857820a34075ece94bbd576eddcdb43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Animals</topic><topic>Bone marrow</topic><topic>Bone Marrow - blood supply</topic><topic>Bone Marrow - ultrastructure</topic><topic>bone marrow blood vessel ossification</topic><topic>Bone Marrow Diseases - blood</topic><topic>Bone Marrow Diseases - pathology</topic><topic>emboli</topic><topic>Extracellular Vesicles - metabolism</topic><topic>Extracellular Vesicles - ultrastructure</topic><topic>Female</topic><topic>human</topic><topic>Humans</topic><topic>Male</topic><topic>Microscopy</topic><topic>Middle Aged</topic><topic>Ossification, Heterotopic - blood</topic><topic>Ossification, Heterotopic - pathology</topic><topic>ossified particles</topic><topic>rat</topic><topic>Rats</topic><topic>Rats, Inbred F344</topic><topic>Vascular Calcification - blood</topic><topic>Vascular Calcification - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Prisby, Rhonda</creatorcontrib><creatorcontrib>Ross, Jean</creatorcontrib><creatorcontrib>Opdenaker, Lynn</creatorcontrib><creatorcontrib>McLane, Mary Ann</creatorcontrib><creatorcontrib>Lee, Seungyong</creatorcontrib><creatorcontrib>Sun, Xiangle</creatorcontrib><creatorcontrib>Guderian, Sophie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Microcirculation (New York, N.Y. 1994)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Prisby, Rhonda</au><au>Ross, Jean</au><au>Opdenaker, Lynn</au><au>McLane, Mary Ann</au><au>Lee, Seungyong</au><au>Sun, Xiangle</au><au>Guderian, Sophie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Discovery of a bone‐like blood particle in the peripheral circulation of humans and rodents</atitle><jtitle>Microcirculation (New York, N.Y. 1994)</jtitle><addtitle>Microcirculation</addtitle><date>2019-11</date><risdate>2019</risdate><volume>26</volume><issue>8</issue><spage>e12579</spage><epage>n/a</epage><pages>e12579-n/a</pages><issn>1073-9688</issn><eissn>1549-8719</eissn><abstract>Objective
To characterize ossified bone marrow blood vessels and confirm the presence of ossified particles (OSP) in humans and rodents.
Methods
Human bone marrow blood vessels were processed for scanning and transmission electron microscopy. Whole blood samples were collected from younger (26‐39 years; n = 6) and older (55‐63 years; n = 6) volunteers and male Fischer‐344 rats (1 month, n = 7; 6 months, n = 7; 12 months, n = 7; 18‐months, n = 6; 24 months, n = 8). OSP in the whole blood samples were sorted and imaged with microscopy to determine diameter, circularity, and solidity. Additionally, the chemical composition of OSP was determined via elemental analysis.
Results
SEM revealed two types of ossified bone marrow blood vessels: that is, “transitioning” and “ossified.” OSP were adhered to the surface of transitioning vessels and theoretically gain access to and circulate within the blood. The majority of OSP were ≤15 μm in diameter, but many were of sufficient size to serve as emboli (ie, >15 μm).OSP were predominately oblong in shape and several had jagged tips and edges.
Conclusions
We introduce a novel, bone‐like blood particle that may be diagnostic of bone marrow blood vessel ossification. Further, OSP may associate with several disease states (eg, atherosclerosis).</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31246334</pmid><doi>10.1111/micc.12579</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-1986-1447</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adult Aged Animals Bone marrow Bone Marrow - blood supply Bone Marrow - ultrastructure bone marrow blood vessel ossification Bone Marrow Diseases - blood Bone Marrow Diseases - pathology emboli Extracellular Vesicles - metabolism Extracellular Vesicles - ultrastructure Female human Humans Male Microscopy Middle Aged Ossification, Heterotopic - blood Ossification, Heterotopic - pathology ossified particles rat Rats Rats, Inbred F344 Vascular Calcification - blood Vascular Calcification - pathology |
| title | Discovery of a bone‐like blood particle in the peripheral circulation of humans and rodents |
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