Radiation and Stemness Phenotype May Influence Individual Breast Cancer Outcomes: The Crucial Role of MMPs and Microenvironment
Breast cancer is the most common cancer in women. Radiotherapy (RT) is one of the mainstay treatments for cancer but in some cases is not effective. Cancer stem cells (CSCs) within the tumor can be responsible for recurrence and metastasis after RT. Matrix metalloproteases (MMPs), regulated mainly b...
Gespeichert in:
| Veröffentlicht in: | Cancers 2019-11, Vol.11 (11), p.1781 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 11 |
| container_start_page | 1781 |
| container_title | Cancers |
| container_volume | 11 |
| creator | Olivares-Urbano, María Auxiliadora Griñán-Lisón, Carmen Ríos-Arrabal, Sandra Artacho-Cordón, Francisco Torralbo, Ana Isabel López-Ruiz, Elena Marchal, Juan Antonio Núñez, María Isabel |
| description | Breast cancer is the most common cancer in women. Radiotherapy (RT) is one of the mainstay treatments for cancer but in some cases is not effective. Cancer stem cells (CSCs) within the tumor can be responsible for recurrence and metastasis after RT. Matrix metalloproteases (MMPs), regulated mainly by tissue inhibitors of metalloproteinases (TIMPs) and histone deacetylases (HDACs), may also contribute to tumor development by modifying its activity after RT. The aim of this work was to study the effects of RT on the expression of MMPs, TIMPs and HDACs on different cell subpopulations in MCF-7, MDA-MB-231 and SK-BR-3 cell lines. We assessed the in vitro expression of these genes in different 3D culture models and induced tumors in female NSG mice by orthotopic xenotransplants. Our results showed that gene expression is related to the cell subpopulation studied, the culture model used and the single radiation dose administered. Moreover, the crucial role played by the microenvironment in terms of cell interactions and CSC plasticity in tumor growth and RT outcome is also shown, supporting the use of higher doses (6 Gy) to achieve better control of tumor development. |
| doi_str_mv | 10.3390/cancers11111781 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6896076</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2547552350</sourcerecordid><originalsourceid>FETCH-LOGICAL-c421t-a2a54faf1379b25cfe84d6c7938ab952e537918ee3258755453269ce30f9edc93</originalsourceid><addsrcrecordid>eNpdUclOHDEQtSJQQMA5t8hSLlwGvLTtNodIySgsEiMQy9nyuKsZo257YnePNKf8ejxsAupSparn9165EPpGyRHnmhw7GxykTDehavoF7TKi2ERKXW29q3fQQc6PpATnVEn1Fe2UzKSUahf9u7GNt4OPAdvQ4NsB-gA54-sFhDisl4Bndo0vQtuNUNRK1fiVb0bb4d8JbB7w9MkFvhoHF3vIJ_huAXiaRucL5iZ2gGOLZ7Pr_CQw8y5FCCufYughDPtou7VdhoOXvIfuT__cTc8nl1dnF9NflxNXMTpMLLOiam1LudJzJlwLddVIpzSv7VwLBqIMaA3AmaiVEJXgTGoHnLQaGqf5Hvr5zLsc533pFOlkO7NMvrdpbaL15uMk-IV5iCsjay2JkoXg8IUgxb8j5MH0PjvoOhsgjtkwTgXRitEN9Mcn6GMcUyjrGSaq4o5xQQrq-BlVPiTnBO2bGUrM5r7m033Li-_vd3jDv16T_wfsiqNr</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2547552350</pqid></control><display><type>article</type><title>Radiation and Stemness Phenotype May Influence Individual Breast Cancer Outcomes: The Crucial Role of MMPs and Microenvironment</title><source>PubMed Central Open Access</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Olivares-Urbano, María Auxiliadora ; Griñán-Lisón, Carmen ; Ríos-Arrabal, Sandra ; Artacho-Cordón, Francisco ; Torralbo, Ana Isabel ; López-Ruiz, Elena ; Marchal, Juan Antonio ; Núñez, María Isabel</creator><creatorcontrib>Olivares-Urbano, María Auxiliadora ; Griñán-Lisón, Carmen ; Ríos-Arrabal, Sandra ; Artacho-Cordón, Francisco ; Torralbo, Ana Isabel ; López-Ruiz, Elena ; Marchal, Juan Antonio ; Núñez, María Isabel</creatorcontrib><description>Breast cancer is the most common cancer in women. Radiotherapy (RT) is one of the mainstay treatments for cancer but in some cases is not effective. Cancer stem cells (CSCs) within the tumor can be responsible for recurrence and metastasis after RT. Matrix metalloproteases (MMPs), regulated mainly by tissue inhibitors of metalloproteinases (TIMPs) and histone deacetylases (HDACs), may also contribute to tumor development by modifying its activity after RT. The aim of this work was to study the effects of RT on the expression of MMPs, TIMPs and HDACs on different cell subpopulations in MCF-7, MDA-MB-231 and SK-BR-3 cell lines. We assessed the in vitro expression of these genes in different 3D culture models and induced tumors in female NSG mice by orthotopic xenotransplants. Our results showed that gene expression is related to the cell subpopulation studied, the culture model used and the single radiation dose administered. Moreover, the crucial role played by the microenvironment in terms of cell interactions and CSC plasticity in tumor growth and RT outcome is also shown, supporting the use of higher doses (6 Gy) to achieve better control of tumor development.</description><identifier>ISSN: 2072-6694</identifier><identifier>EISSN: 2072-6694</identifier><identifier>DOI: 10.3390/cancers11111781</identifier><identifier>PMID: 31726667</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Angiogenesis ; Apoptosis ; Breast cancer ; Cancer therapies ; Cell culture ; Cell cycle ; Cell growth ; Cell interactions ; Enzymes ; Gene expression ; Histones ; Medical prognosis ; Metastases ; Metastasis ; Microenvironments ; Phenotypes ; Radiation ; Radiation therapy ; Stem cell transplantation ; Stem cells ; Tissue inhibitor of metalloproteinases ; Tumors ; Xenografts</subject><ispartof>Cancers, 2019-11, Vol.11 (11), p.1781</ispartof><rights>2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 by the authors. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-a2a54faf1379b25cfe84d6c7938ab952e537918ee3258755453269ce30f9edc93</citedby><cites>FETCH-LOGICAL-c421t-a2a54faf1379b25cfe84d6c7938ab952e537918ee3258755453269ce30f9edc93</cites><orcidid>0000-0003-3853-6292 ; 0000-0001-8159-7969 ; 0000-0002-4996-8261</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896076/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896076/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31726667$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Olivares-Urbano, María Auxiliadora</creatorcontrib><creatorcontrib>Griñán-Lisón, Carmen</creatorcontrib><creatorcontrib>Ríos-Arrabal, Sandra</creatorcontrib><creatorcontrib>Artacho-Cordón, Francisco</creatorcontrib><creatorcontrib>Torralbo, Ana Isabel</creatorcontrib><creatorcontrib>López-Ruiz, Elena</creatorcontrib><creatorcontrib>Marchal, Juan Antonio</creatorcontrib><creatorcontrib>Núñez, María Isabel</creatorcontrib><title>Radiation and Stemness Phenotype May Influence Individual Breast Cancer Outcomes: The Crucial Role of MMPs and Microenvironment</title><title>Cancers</title><addtitle>Cancers (Basel)</addtitle><description>Breast cancer is the most common cancer in women. Radiotherapy (RT) is one of the mainstay treatments for cancer but in some cases is not effective. Cancer stem cells (CSCs) within the tumor can be responsible for recurrence and metastasis after RT. Matrix metalloproteases (MMPs), regulated mainly by tissue inhibitors of metalloproteinases (TIMPs) and histone deacetylases (HDACs), may also contribute to tumor development by modifying its activity after RT. The aim of this work was to study the effects of RT on the expression of MMPs, TIMPs and HDACs on different cell subpopulations in MCF-7, MDA-MB-231 and SK-BR-3 cell lines. We assessed the in vitro expression of these genes in different 3D culture models and induced tumors in female NSG mice by orthotopic xenotransplants. Our results showed that gene expression is related to the cell subpopulation studied, the culture model used and the single radiation dose administered. Moreover, the crucial role played by the microenvironment in terms of cell interactions and CSC plasticity in tumor growth and RT outcome is also shown, supporting the use of higher doses (6 Gy) to achieve better control of tumor development.</description><subject>Angiogenesis</subject><subject>Apoptosis</subject><subject>Breast cancer</subject><subject>Cancer therapies</subject><subject>Cell culture</subject><subject>Cell cycle</subject><subject>Cell growth</subject><subject>Cell interactions</subject><subject>Enzymes</subject><subject>Gene expression</subject><subject>Histones</subject><subject>Medical prognosis</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Microenvironments</subject><subject>Phenotypes</subject><subject>Radiation</subject><subject>Radiation therapy</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Tissue inhibitor of metalloproteinases</subject><subject>Tumors</subject><subject>Xenografts</subject><issn>2072-6694</issn><issn>2072-6694</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdUclOHDEQtSJQQMA5t8hSLlwGvLTtNodIySgsEiMQy9nyuKsZo257YnePNKf8ejxsAupSparn9165EPpGyRHnmhw7GxykTDehavoF7TKi2ERKXW29q3fQQc6PpATnVEn1Fe2UzKSUahf9u7GNt4OPAdvQ4NsB-gA54-sFhDisl4Bndo0vQtuNUNRK1fiVb0bb4d8JbB7w9MkFvhoHF3vIJ_huAXiaRucL5iZ2gGOLZ7Pr_CQw8y5FCCufYughDPtou7VdhoOXvIfuT__cTc8nl1dnF9NflxNXMTpMLLOiam1LudJzJlwLddVIpzSv7VwLBqIMaA3AmaiVEJXgTGoHnLQaGqf5Hvr5zLsc533pFOlkO7NMvrdpbaL15uMk-IV5iCsjay2JkoXg8IUgxb8j5MH0PjvoOhsgjtkwTgXRitEN9Mcn6GMcUyjrGSaq4o5xQQrq-BlVPiTnBO2bGUrM5r7m033Li-_vd3jDv16T_wfsiqNr</recordid><startdate>20191112</startdate><enddate>20191112</enddate><creator>Olivares-Urbano, María Auxiliadora</creator><creator>Griñán-Lisón, Carmen</creator><creator>Ríos-Arrabal, Sandra</creator><creator>Artacho-Cordón, Francisco</creator><creator>Torralbo, Ana Isabel</creator><creator>López-Ruiz, Elena</creator><creator>Marchal, Juan Antonio</creator><creator>Núñez, María Isabel</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TO</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3853-6292</orcidid><orcidid>https://orcid.org/0000-0001-8159-7969</orcidid><orcidid>https://orcid.org/0000-0002-4996-8261</orcidid></search><sort><creationdate>20191112</creationdate><title>Radiation and Stemness Phenotype May Influence Individual Breast Cancer Outcomes: The Crucial Role of MMPs and Microenvironment</title><author>Olivares-Urbano, María Auxiliadora ; Griñán-Lisón, Carmen ; Ríos-Arrabal, Sandra ; Artacho-Cordón, Francisco ; Torralbo, Ana Isabel ; López-Ruiz, Elena ; Marchal, Juan Antonio ; Núñez, María Isabel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-a2a54faf1379b25cfe84d6c7938ab952e537918ee3258755453269ce30f9edc93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Angiogenesis</topic><topic>Apoptosis</topic><topic>Breast cancer</topic><topic>Cancer therapies</topic><topic>Cell culture</topic><topic>Cell cycle</topic><topic>Cell growth</topic><topic>Cell interactions</topic><topic>Enzymes</topic><topic>Gene expression</topic><topic>Histones</topic><topic>Medical prognosis</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>Microenvironments</topic><topic>Phenotypes</topic><topic>Radiation</topic><topic>Radiation therapy</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Tissue inhibitor of metalloproteinases</topic><topic>Tumors</topic><topic>Xenografts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Olivares-Urbano, María Auxiliadora</creatorcontrib><creatorcontrib>Griñán-Lisón, Carmen</creatorcontrib><creatorcontrib>Ríos-Arrabal, Sandra</creatorcontrib><creatorcontrib>Artacho-Cordón, Francisco</creatorcontrib><creatorcontrib>Torralbo, Ana Isabel</creatorcontrib><creatorcontrib>López-Ruiz, Elena</creatorcontrib><creatorcontrib>Marchal, Juan Antonio</creatorcontrib><creatorcontrib>Núñez, María Isabel</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Olivares-Urbano, María Auxiliadora</au><au>Griñán-Lisón, Carmen</au><au>Ríos-Arrabal, Sandra</au><au>Artacho-Cordón, Francisco</au><au>Torralbo, Ana Isabel</au><au>López-Ruiz, Elena</au><au>Marchal, Juan Antonio</au><au>Núñez, María Isabel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Radiation and Stemness Phenotype May Influence Individual Breast Cancer Outcomes: The Crucial Role of MMPs and Microenvironment</atitle><jtitle>Cancers</jtitle><addtitle>Cancers (Basel)</addtitle><date>2019-11-12</date><risdate>2019</risdate><volume>11</volume><issue>11</issue><spage>1781</spage><pages>1781-</pages><issn>2072-6694</issn><eissn>2072-6694</eissn><abstract>Breast cancer is the most common cancer in women. Radiotherapy (RT) is one of the mainstay treatments for cancer but in some cases is not effective. Cancer stem cells (CSCs) within the tumor can be responsible for recurrence and metastasis after RT. Matrix metalloproteases (MMPs), regulated mainly by tissue inhibitors of metalloproteinases (TIMPs) and histone deacetylases (HDACs), may also contribute to tumor development by modifying its activity after RT. The aim of this work was to study the effects of RT on the expression of MMPs, TIMPs and HDACs on different cell subpopulations in MCF-7, MDA-MB-231 and SK-BR-3 cell lines. We assessed the in vitro expression of these genes in different 3D culture models and induced tumors in female NSG mice by orthotopic xenotransplants. Our results showed that gene expression is related to the cell subpopulation studied, the culture model used and the single radiation dose administered. Moreover, the crucial role played by the microenvironment in terms of cell interactions and CSC plasticity in tumor growth and RT outcome is also shown, supporting the use of higher doses (6 Gy) to achieve better control of tumor development.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>31726667</pmid><doi>10.3390/cancers11111781</doi><orcidid>https://orcid.org/0000-0003-3853-6292</orcidid><orcidid>https://orcid.org/0000-0001-8159-7969</orcidid><orcidid>https://orcid.org/0000-0002-4996-8261</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2072-6694 |
| ispartof | Cancers, 2019-11, Vol.11 (11), p.1781 |
| issn | 2072-6694 2072-6694 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6896076 |
| source | PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Angiogenesis Apoptosis Breast cancer Cancer therapies Cell culture Cell cycle Cell growth Cell interactions Enzymes Gene expression Histones Medical prognosis Metastases Metastasis Microenvironments Phenotypes Radiation Radiation therapy Stem cell transplantation Stem cells Tissue inhibitor of metalloproteinases Tumors Xenografts |
| title | Radiation and Stemness Phenotype May Influence Individual Breast Cancer Outcomes: The Crucial Role of MMPs and Microenvironment |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T20%3A40%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Radiation%20and%20Stemness%20Phenotype%20May%20Influence%20Individual%20Breast%20Cancer%20Outcomes:%20The%20Crucial%20Role%20of%20MMPs%20and%20Microenvironment&rft.jtitle=Cancers&rft.au=Olivares-Urbano,%20Mar%C3%ADa%20Auxiliadora&rft.date=2019-11-12&rft.volume=11&rft.issue=11&rft.spage=1781&rft.pages=1781-&rft.issn=2072-6694&rft.eissn=2072-6694&rft_id=info:doi/10.3390/cancers11111781&rft_dat=%3Cproquest_pubme%3E2547552350%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2547552350&rft_id=info:pmid/31726667&rfr_iscdi=true |