The Potential Selective Cytotoxicity of Poly (L- Lactic Acid)-Based Scaffolds Functionalized with Nanohydroxyapatite and Europium (III) Ions toward Osteosarcoma Cells
Osteosarcoma (OSA) is malignant bone tumor, occurring in children and adults, characterized by poor prognosis. Despite advances in chemotherapy and surgical techniques, the survival of osteosarcoma patients is not improving significantly. Currently, great efforts are taken to identify novel selectiv...
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| Veröffentlicht in: | Materials 2019-11, Vol.12 (22), p.3779 |
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| description | Osteosarcoma (OSA) is malignant bone tumor, occurring in children and adults, characterized by poor prognosis. Despite advances in chemotherapy and surgical techniques, the survival of osteosarcoma patients is not improving significantly. Currently, great efforts are taken to identify novel selective strategies, distinguishing between cancer and normal cells. This includes development of biomimetic scaffolds with anticancer properties that can simultaneously support and modulate proper regeneration of bone tissue. In this study cytotoxicity of scaffolds composed from poly (L-lactic acid) functionalized with nanohydroxyapatite (nHAp) and doped with europium (III) ions-10 wt % 3 mol % Eu
: nHAp@PLLA was tested using human osteosarcoma cells: U-2 OS, Saos-2 and MG-63. Human adipose tissue-derived stromal cells (HuASCs) were used as non-transformed cells to determine the selective cytotoxicity of the carrier. Analysis included evaluation of cells morphology (confocal/scanning electron microscopy (SEM)), metabolic activity and apoptosis profile in cultures on the scaffolds. Results obtained indicated on high cytotoxicity of scaffolds toward all OSA cell lines, associated with a decrease of cells' viability, deterioration of metabolic activity and activation of apoptotic factors determined at mRNA and miRNA levels. Simultaneously, the biomaterials did not affect HuASCs' viability and proliferation rate. Obtained scaffolds showed a bioimaging function, due to functionalization with luminescent europium ions, and thus may find application in theranostics treatment of OSA. |
| doi_str_mv | 10.3390/ma12223779 |
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: nHAp@PLLA was tested using human osteosarcoma cells: U-2 OS, Saos-2 and MG-63. Human adipose tissue-derived stromal cells (HuASCs) were used as non-transformed cells to determine the selective cytotoxicity of the carrier. Analysis included evaluation of cells morphology (confocal/scanning electron microscopy (SEM)), metabolic activity and apoptosis profile in cultures on the scaffolds. Results obtained indicated on high cytotoxicity of scaffolds toward all OSA cell lines, associated with a decrease of cells' viability, deterioration of metabolic activity and activation of apoptotic factors determined at mRNA and miRNA levels. Simultaneously, the biomaterials did not affect HuASCs' viability and proliferation rate. Obtained scaffolds showed a bioimaging function, due to functionalization with luminescent europium ions, and thus may find application in theranostics treatment of OSA.</description><identifier>ISSN: 1996-1944</identifier><identifier>EISSN: 1996-1944</identifier><identifier>DOI: 10.3390/ma12223779</identifier><identifier>PMID: 31752084</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Adipose tissue ; Anticancer properties ; Apoptosis ; Biocompatibility ; Biomedical materials ; Biomimetics ; Bone cancer ; Bones ; Cancer therapies ; Cell culture ; Chemotherapy ; Cytotoxicity ; Drug delivery systems ; Europium ; Hydroxyapatite ; Lactic acid ; Medical imaging ; Metabolism ; Morphology ; Regeneration ; Scaffolds ; Toxicity</subject><ispartof>Materials, 2019-11, Vol.12 (22), p.3779</ispartof><rights>2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 by the authors. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-156704dcf060e690884868b2776d7107a4c71a790a81d6732ac548cb08141c0f3</citedby><cites>FETCH-LOGICAL-c406t-156704dcf060e690884868b2776d7107a4c71a790a81d6732ac548cb08141c0f3</cites><orcidid>0000-0001-8458-1532 ; 0000-0002-7314-9821</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888250/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888250/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31752084$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sikora, Mateusz</creatorcontrib><creatorcontrib>Marcinkowska, Klaudia</creatorcontrib><creatorcontrib>Marycz, Krzysztof</creatorcontrib><creatorcontrib>Wiglusz, Rafał Jakub</creatorcontrib><creatorcontrib>Śmieszek, Agnieszka</creatorcontrib><title>The Potential Selective Cytotoxicity of Poly (L- Lactic Acid)-Based Scaffolds Functionalized with Nanohydroxyapatite and Europium (III) Ions toward Osteosarcoma Cells</title><title>Materials</title><addtitle>Materials (Basel)</addtitle><description>Osteosarcoma (OSA) is malignant bone tumor, occurring in children and adults, characterized by poor prognosis. Despite advances in chemotherapy and surgical techniques, the survival of osteosarcoma patients is not improving significantly. Currently, great efforts are taken to identify novel selective strategies, distinguishing between cancer and normal cells. This includes development of biomimetic scaffolds with anticancer properties that can simultaneously support and modulate proper regeneration of bone tissue. In this study cytotoxicity of scaffolds composed from poly (L-lactic acid) functionalized with nanohydroxyapatite (nHAp) and doped with europium (III) ions-10 wt % 3 mol % Eu
: nHAp@PLLA was tested using human osteosarcoma cells: U-2 OS, Saos-2 and MG-63. Human adipose tissue-derived stromal cells (HuASCs) were used as non-transformed cells to determine the selective cytotoxicity of the carrier. Analysis included evaluation of cells morphology (confocal/scanning electron microscopy (SEM)), metabolic activity and apoptosis profile in cultures on the scaffolds. Results obtained indicated on high cytotoxicity of scaffolds toward all OSA cell lines, associated with a decrease of cells' viability, deterioration of metabolic activity and activation of apoptotic factors determined at mRNA and miRNA levels. Simultaneously, the biomaterials did not affect HuASCs' viability and proliferation rate. Obtained scaffolds showed a bioimaging function, due to functionalization with luminescent europium ions, and thus may find application in theranostics treatment of OSA.</description><subject>Adipose tissue</subject><subject>Anticancer properties</subject><subject>Apoptosis</subject><subject>Biocompatibility</subject><subject>Biomedical materials</subject><subject>Biomimetics</subject><subject>Bone cancer</subject><subject>Bones</subject><subject>Cancer therapies</subject><subject>Cell culture</subject><subject>Chemotherapy</subject><subject>Cytotoxicity</subject><subject>Drug delivery systems</subject><subject>Europium</subject><subject>Hydroxyapatite</subject><subject>Lactic acid</subject><subject>Medical imaging</subject><subject>Metabolism</subject><subject>Morphology</subject><subject>Regeneration</subject><subject>Scaffolds</subject><subject>Toxicity</subject><issn>1996-1944</issn><issn>1996-1944</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkl1rFDEYhYMottTe-AMk4M1WGM3HbD5uhHZpdWCxQuv18G6ScVNmJmuSaTv-IH-nKa21mpsEzsPhvCcvQq8pec-5Jh8GoIwxLqV-hvap1qKiuq6fP3nvocOUrkg5nFPF9Eu0x6lcMqLqffTrcuvw15DdmD30-ML1zmR_7fBqziGHW298nnHoCtPPeLGu8BoKYPCx8faoOoHkLL4w0HWhtwmfTWNRwwi9_1mEG5-3-AuMYTvbGG5n2EH22WEYLT6dYtj5acCLpmmOcBPGhHO4gWjxecouJIgmDIBXru_TK_Sigz65w4f7AH07O71cfa7W55-a1fG6MjURuaJLIUltTUcEcUITpWol1IZJKaykREJtJAWpCShqheQMzLJWZkMUrakhHT9AH-99d9NmcNaUWiL07S76AeLcBvDtv8rot-33cN0KpRRbkmKweDCI4cfkUm4Hn0wZAUYXptSyu-q10FoW9O1_6FWYYqmuUCWVZJRrWqh395SJIaXouscwlLR3G9D-3YACv3ka_xH989_8N5oLrHY</recordid><startdate>20191118</startdate><enddate>20191118</enddate><creator>Sikora, Mateusz</creator><creator>Marcinkowska, Klaudia</creator><creator>Marycz, Krzysztof</creator><creator>Wiglusz, Rafał Jakub</creator><creator>Śmieszek, Agnieszka</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-8458-1532</orcidid><orcidid>https://orcid.org/0000-0002-7314-9821</orcidid></search><sort><creationdate>20191118</creationdate><title>The Potential Selective Cytotoxicity of Poly (L- Lactic Acid)-Based Scaffolds Functionalized with Nanohydroxyapatite and Europium (III) Ions toward Osteosarcoma Cells</title><author>Sikora, Mateusz ; Marcinkowska, Klaudia ; Marycz, Krzysztof ; Wiglusz, Rafał Jakub ; Śmieszek, Agnieszka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-156704dcf060e690884868b2776d7107a4c71a790a81d6732ac548cb08141c0f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adipose tissue</topic><topic>Anticancer properties</topic><topic>Apoptosis</topic><topic>Biocompatibility</topic><topic>Biomedical materials</topic><topic>Biomimetics</topic><topic>Bone cancer</topic><topic>Bones</topic><topic>Cancer therapies</topic><topic>Cell culture</topic><topic>Chemotherapy</topic><topic>Cytotoxicity</topic><topic>Drug delivery systems</topic><topic>Europium</topic><topic>Hydroxyapatite</topic><topic>Lactic acid</topic><topic>Medical imaging</topic><topic>Metabolism</topic><topic>Morphology</topic><topic>Regeneration</topic><topic>Scaffolds</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sikora, Mateusz</creatorcontrib><creatorcontrib>Marcinkowska, Klaudia</creatorcontrib><creatorcontrib>Marycz, Krzysztof</creatorcontrib><creatorcontrib>Wiglusz, Rafał Jakub</creatorcontrib><creatorcontrib>Śmieszek, Agnieszka</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sikora, Mateusz</au><au>Marcinkowska, Klaudia</au><au>Marycz, Krzysztof</au><au>Wiglusz, Rafał Jakub</au><au>Śmieszek, Agnieszka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Potential Selective Cytotoxicity of Poly (L- Lactic Acid)-Based Scaffolds Functionalized with Nanohydroxyapatite and Europium (III) Ions toward Osteosarcoma Cells</atitle><jtitle>Materials</jtitle><addtitle>Materials (Basel)</addtitle><date>2019-11-18</date><risdate>2019</risdate><volume>12</volume><issue>22</issue><spage>3779</spage><pages>3779-</pages><issn>1996-1944</issn><eissn>1996-1944</eissn><abstract>Osteosarcoma (OSA) is malignant bone tumor, occurring in children and adults, characterized by poor prognosis. 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: nHAp@PLLA was tested using human osteosarcoma cells: U-2 OS, Saos-2 and MG-63. Human adipose tissue-derived stromal cells (HuASCs) were used as non-transformed cells to determine the selective cytotoxicity of the carrier. Analysis included evaluation of cells morphology (confocal/scanning electron microscopy (SEM)), metabolic activity and apoptosis profile in cultures on the scaffolds. Results obtained indicated on high cytotoxicity of scaffolds toward all OSA cell lines, associated with a decrease of cells' viability, deterioration of metabolic activity and activation of apoptotic factors determined at mRNA and miRNA levels. Simultaneously, the biomaterials did not affect HuASCs' viability and proliferation rate. Obtained scaffolds showed a bioimaging function, due to functionalization with luminescent europium ions, and thus may find application in theranostics treatment of OSA.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>31752084</pmid><doi>10.3390/ma12223779</doi><orcidid>https://orcid.org/0000-0001-8458-1532</orcidid><orcidid>https://orcid.org/0000-0002-7314-9821</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adipose tissue Anticancer properties Apoptosis Biocompatibility Biomedical materials Biomimetics Bone cancer Bones Cancer therapies Cell culture Chemotherapy Cytotoxicity Drug delivery systems Europium Hydroxyapatite Lactic acid Medical imaging Metabolism Morphology Regeneration Scaffolds Toxicity |
| title | The Potential Selective Cytotoxicity of Poly (L- Lactic Acid)-Based Scaffolds Functionalized with Nanohydroxyapatite and Europium (III) Ions toward Osteosarcoma Cells |
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