Immunization with recombinant enolase of Sporothrix spp. (rSsEno) confers effective protection against sporotrichosis in mice
In recent years, research has focused on the immunoreactive components of the Sporothrix schenckii cell wall that can be relevant targets for preventive and therapeutic vaccines against sporotrichosis, an emergent worldwide mycosis. In a previous study, we identified a 47-kDa enolase as an immunodom...
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| creator | Portuondo, Deivys Leandro Dores-Silva, Paulo Roberto Ferreira, Lucas Souza de Oliveira, Carlos S. Téllez-Martínez, Damiana Marcos, Caroline Maria de Aguiar Loesch, Maria Luiza Guzmán, Fanny Gava, Lisandra M. Borges, Júlio César Pereira, Sandro Antonio Batista-Duharte, Alexander Carlos, Iracilda Zeppone |
| description | In recent years, research has focused on the immunoreactive components of the
Sporothrix schenckii
cell wall that can be relevant targets for preventive and therapeutic vaccines against sporotrichosis, an emergent worldwide mycosis. In a previous study, we identified a 47-kDa enolase as an immunodominant antigen in mice vaccinated with an adjuvanted mixture of
S. schenckii
cell wall proteins. Here, we sought to assess the protective potential of a
Sporothrix
spp. recombinant enolase (rSsEno) formulated with or without the adjuvant Montanide Pet-GelA (PGA) against the
S. brasiliensis
infection in mice. Mice that were immunized with rSsEno plus PGA showed increased antibody titters against rSsEno and increased median survival time when challenged with
S. brasiliensis
as compared with mice that had not been immunized or that were immunized with rSsEno alone. Immunization with rSsEno plus PGA induced a predominantly T-helper 1 cytokine pattern after
in vitro
stimulation of splenic cells with rSsEno: elevated levels of IFN-γ and IL-2, as well as of other cytokines involved in host defense against sporotrichosis, such as TNF-alpha, IL-6, and IL-4. Furthermore, we show for the first time the presence of enolase in the cell wall of both
S. schenckii
and
S. brasiliensis
. As a whole, our results suggest that enolase could be used as a potential antigenic target for vaccinal purposes against sporotrichosis. |
| doi_str_mv | 10.1038/s41598-019-53135-z |
| format | Article |
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Sporothrix schenckii
cell wall that can be relevant targets for preventive and therapeutic vaccines against sporotrichosis, an emergent worldwide mycosis. In a previous study, we identified a 47-kDa enolase as an immunodominant antigen in mice vaccinated with an adjuvanted mixture of
S. schenckii
cell wall proteins. Here, we sought to assess the protective potential of a
Sporothrix
spp. recombinant enolase (rSsEno) formulated with or without the adjuvant Montanide Pet-GelA (PGA) against the
S. brasiliensis
infection in mice. Mice that were immunized with rSsEno plus PGA showed increased antibody titters against rSsEno and increased median survival time when challenged with
S. brasiliensis
as compared with mice that had not been immunized or that were immunized with rSsEno alone. Immunization with rSsEno plus PGA induced a predominantly T-helper 1 cytokine pattern after
in vitro
stimulation of splenic cells with rSsEno: elevated levels of IFN-γ and IL-2, as well as of other cytokines involved in host defense against sporotrichosis, such as TNF-alpha, IL-6, and IL-4. Furthermore, we show for the first time the presence of enolase in the cell wall of both
S. schenckii
and
S. brasiliensis
. As a whole, our results suggest that enolase could be used as a potential antigenic target for vaccinal purposes against sporotrichosis.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-53135-z</identifier><identifier>PMID: 31748544</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>101/28 ; 631/250/590/2294 ; 64/60 ; 692/420/254 ; 82/1 ; 82/16 ; 82/29 ; 82/80 ; 82/83 ; 96/21 ; 96/31 ; Amino Acid Sequence ; Animals ; Antibodies, Fungal - immunology ; Cell walls ; Cytokines - metabolism ; Fungal Proteins - administration & dosage ; Fungal Proteins - immunology ; Humanities and Social Sciences ; Immunity, Cellular - immunology ; Immunization ; Interleukin 2 ; Interleukin 4 ; Interleukin 6 ; Male ; Mice ; Mice, Inbred BALB C ; multidisciplinary ; Mycosis ; Phosphopyruvate hydratase ; Phosphopyruvate Hydratase - administration & dosage ; Phosphopyruvate Hydratase - immunology ; Recombinant Proteins - administration & dosage ; Recombinant Proteins - immunology ; Science ; Science (multidisciplinary) ; Sequence Homology ; Spleen ; Sporothrix ; Sporothrix - enzymology ; Sporothrix - immunology ; Sporotrichosis ; Sporotrichosis - immunology ; Sporotrichosis - microbiology ; Sporotrichosis - prevention & control ; Tumor necrosis factor ; γ-Interferon</subject><ispartof>Scientific reports, 2019-11, Vol.9 (1), p.17179-14, Article 17179</ispartof><rights>The Author(s) 2019</rights><rights>2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-674631efab47096313cca86a100d07c2c73b00d2deb40f16e0038ce87d9ae8ed3</citedby><cites>FETCH-LOGICAL-c474t-674631efab47096313cca86a100d07c2c73b00d2deb40f16e0038ce87d9ae8ed3</cites><orcidid>0000-0002-0084-3468 ; 0000-0002-5437-4038 ; 0000-0003-2678-0431 ; 0000-0001-6957-094X ; 0000-0001-9306-7404 ; 0000-0003-1537-0904 ; 0000-0003-2282-7562 ; 0000-0003-4856-748X ; 0000-0002-1875-0518 ; 0000-0001-9920-0106</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6868355/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6868355/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31748544$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Portuondo, Deivys Leandro</creatorcontrib><creatorcontrib>Dores-Silva, Paulo Roberto</creatorcontrib><creatorcontrib>Ferreira, Lucas Souza</creatorcontrib><creatorcontrib>de Oliveira, Carlos S.</creatorcontrib><creatorcontrib>Téllez-Martínez, Damiana</creatorcontrib><creatorcontrib>Marcos, Caroline Maria</creatorcontrib><creatorcontrib>de Aguiar Loesch, Maria Luiza</creatorcontrib><creatorcontrib>Guzmán, Fanny</creatorcontrib><creatorcontrib>Gava, Lisandra M.</creatorcontrib><creatorcontrib>Borges, Júlio César</creatorcontrib><creatorcontrib>Pereira, Sandro Antonio</creatorcontrib><creatorcontrib>Batista-Duharte, Alexander</creatorcontrib><creatorcontrib>Carlos, Iracilda Zeppone</creatorcontrib><title>Immunization with recombinant enolase of Sporothrix spp. (rSsEno) confers effective protection against sporotrichosis in mice</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>In recent years, research has focused on the immunoreactive components of the
Sporothrix schenckii
cell wall that can be relevant targets for preventive and therapeutic vaccines against sporotrichosis, an emergent worldwide mycosis. In a previous study, we identified a 47-kDa enolase as an immunodominant antigen in mice vaccinated with an adjuvanted mixture of
S. schenckii
cell wall proteins. Here, we sought to assess the protective potential of a
Sporothrix
spp. recombinant enolase (rSsEno) formulated with or without the adjuvant Montanide Pet-GelA (PGA) against the
S. brasiliensis
infection in mice. Mice that were immunized with rSsEno plus PGA showed increased antibody titters against rSsEno and increased median survival time when challenged with
S. brasiliensis
as compared with mice that had not been immunized or that were immunized with rSsEno alone. Immunization with rSsEno plus PGA induced a predominantly T-helper 1 cytokine pattern after
in vitro
stimulation of splenic cells with rSsEno: elevated levels of IFN-γ and IL-2, as well as of other cytokines involved in host defense against sporotrichosis, such as TNF-alpha, IL-6, and IL-4. Furthermore, we show for the first time the presence of enolase in the cell wall of both
S. schenckii
and
S. brasiliensis
. As a whole, our results suggest that enolase could be used as a potential antigenic target for vaccinal purposes against sporotrichosis.</description><subject>101/28</subject><subject>631/250/590/2294</subject><subject>64/60</subject><subject>692/420/254</subject><subject>82/1</subject><subject>82/16</subject><subject>82/29</subject><subject>82/80</subject><subject>82/83</subject><subject>96/21</subject><subject>96/31</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Fungal - immunology</subject><subject>Cell walls</subject><subject>Cytokines - metabolism</subject><subject>Fungal Proteins - administration & dosage</subject><subject>Fungal Proteins - immunology</subject><subject>Humanities and Social Sciences</subject><subject>Immunity, Cellular - immunology</subject><subject>Immunization</subject><subject>Interleukin 2</subject><subject>Interleukin 4</subject><subject>Interleukin 6</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>multidisciplinary</subject><subject>Mycosis</subject><subject>Phosphopyruvate hydratase</subject><subject>Phosphopyruvate Hydratase - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Portuondo, Deivys Leandro</au><au>Dores-Silva, Paulo Roberto</au><au>Ferreira, Lucas Souza</au><au>de Oliveira, Carlos S.</au><au>Téllez-Martínez, Damiana</au><au>Marcos, Caroline Maria</au><au>de Aguiar Loesch, Maria Luiza</au><au>Guzmán, Fanny</au><au>Gava, Lisandra M.</au><au>Borges, Júlio César</au><au>Pereira, Sandro Antonio</au><au>Batista-Duharte, Alexander</au><au>Carlos, Iracilda Zeppone</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunization with recombinant enolase of Sporothrix spp. (rSsEno) confers effective protection against sporotrichosis in mice</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2019-11-20</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>17179</spage><epage>14</epage><pages>17179-14</pages><artnum>17179</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>In recent years, research has focused on the immunoreactive components of the
Sporothrix schenckii
cell wall that can be relevant targets for preventive and therapeutic vaccines against sporotrichosis, an emergent worldwide mycosis. In a previous study, we identified a 47-kDa enolase as an immunodominant antigen in mice vaccinated with an adjuvanted mixture of
S. schenckii
cell wall proteins. Here, we sought to assess the protective potential of a
Sporothrix
spp. recombinant enolase (rSsEno) formulated with or without the adjuvant Montanide Pet-GelA (PGA) against the
S. brasiliensis
infection in mice. Mice that were immunized with rSsEno plus PGA showed increased antibody titters against rSsEno and increased median survival time when challenged with
S. brasiliensis
as compared with mice that had not been immunized or that were immunized with rSsEno alone. Immunization with rSsEno plus PGA induced a predominantly T-helper 1 cytokine pattern after
in vitro
stimulation of splenic cells with rSsEno: elevated levels of IFN-γ and IL-2, as well as of other cytokines involved in host defense against sporotrichosis, such as TNF-alpha, IL-6, and IL-4. Furthermore, we show for the first time the presence of enolase in the cell wall of both
S. schenckii
and
S. brasiliensis
. As a whole, our results suggest that enolase could be used as a potential antigenic target for vaccinal purposes against sporotrichosis.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31748544</pmid><doi>10.1038/s41598-019-53135-z</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-0084-3468</orcidid><orcidid>https://orcid.org/0000-0002-5437-4038</orcidid><orcidid>https://orcid.org/0000-0003-2678-0431</orcidid><orcidid>https://orcid.org/0000-0001-6957-094X</orcidid><orcidid>https://orcid.org/0000-0001-9306-7404</orcidid><orcidid>https://orcid.org/0000-0003-1537-0904</orcidid><orcidid>https://orcid.org/0000-0003-2282-7562</orcidid><orcidid>https://orcid.org/0000-0003-4856-748X</orcidid><orcidid>https://orcid.org/0000-0002-1875-0518</orcidid><orcidid>https://orcid.org/0000-0001-9920-0106</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2019-11, Vol.9 (1), p.17179-14, Article 17179 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6868355 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Springer Nature OA Free Journals; Nature Free; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 101/28 631/250/590/2294 64/60 692/420/254 82/1 82/16 82/29 82/80 82/83 96/21 96/31 Amino Acid Sequence Animals Antibodies, Fungal - immunology Cell walls Cytokines - metabolism Fungal Proteins - administration & dosage Fungal Proteins - immunology Humanities and Social Sciences Immunity, Cellular - immunology Immunization Interleukin 2 Interleukin 4 Interleukin 6 Male Mice Mice, Inbred BALB C multidisciplinary Mycosis Phosphopyruvate hydratase Phosphopyruvate Hydratase - administration & dosage Phosphopyruvate Hydratase - immunology Recombinant Proteins - administration & dosage Recombinant Proteins - immunology Science Science (multidisciplinary) Sequence Homology Spleen Sporothrix Sporothrix - enzymology Sporothrix - immunology Sporotrichosis Sporotrichosis - immunology Sporotrichosis - microbiology Sporotrichosis - prevention & control Tumor necrosis factor γ-Interferon |
| title | Immunization with recombinant enolase of Sporothrix spp. (rSsEno) confers effective protection against sporotrichosis in mice |
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