A Smartphone-based Diffusometric Immunoassay for Detecting C-Reactive Protein
In this study, we developed a portable smartphone-based diffusometry for analyzing the C-reactive protein (CRP) concentration. An optimized fluorescence microscopic add-on system for a smartphone was used to image the 300 nm fluorescent beads. Sequential nanobead images were recorded for a period an...
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| Veröffentlicht in: | Scientific reports 2019-11, Vol.9 (1), p.17131-7, Article 17131 |
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| creator | Chuang, Chih-Shen Deng, Chih-Zong Fang, Yi-Fan Jiang, Hong-Ren Tseng, Pao-Wei Sheen, Horn-Jiunn Fan, Yu-Jui |
| description | In this study, we developed a portable smartphone-based diffusometry for analyzing the C-reactive protein (CRP) concentration. An optimized fluorescence microscopic add-on system for a smartphone was used to image the 300 nm fluorescent beads. Sequential nanobead images were recorded for a period and the image data were used for fluorescence correlation spectrometric (FCS) analysis. Through the analysis, the nanobeads’ diffusion coefficient was obtained. Further, the diffusion coefficients of the anti-CRP-coated nanobeads, which were suspended in the samples with various CRP concentrations, were estimated using smartphone-based diffusometry. After 10 min of reaction, the anti-CRP-coated nanobeads in a higher CRP concentration solution led to a lower diffusion coefficient. Based on the experiments, a linear sensing range of 1~8 µg/mL was found. |
| doi_str_mv | 10.1038/s41598-019-52285-4 |
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An optimized fluorescence microscopic add-on system for a smartphone was used to image the 300 nm fluorescent beads. Sequential nanobead images were recorded for a period and the image data were used for fluorescence correlation spectrometric (FCS) analysis. Through the analysis, the nanobeads’ diffusion coefficient was obtained. Further, the diffusion coefficients of the anti-CRP-coated nanobeads, which were suspended in the samples with various CRP concentrations, were estimated using smartphone-based diffusometry. After 10 min of reaction, the anti-CRP-coated nanobeads in a higher CRP concentration solution led to a lower diffusion coefficient. Based on the experiments, a linear sensing range of 1~8 µg/mL was found.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-52285-4</identifier><identifier>PMID: 31748592</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/1647/2196/2197 ; 692/700 ; 9/10 ; 96/63 ; Biosensing Techniques - instrumentation ; Biosensing Techniques - methods ; C-reactive protein ; C-Reactive Protein - chemistry ; Diffusion coefficient ; Fluorescence ; Humanities and Social Sciences ; Immunoassay - instrumentation ; Immunoassay - methods ; Immunologic Tests - instrumentation ; Immunologic Tests - methods ; multidisciplinary ; Nanoparticles - chemistry ; Science ; Science (multidisciplinary) ; Smartphone ; Smartphones ; Spectrometry, Fluorescence - instrumentation ; Spectrometry, Fluorescence - methods</subject><ispartof>Scientific reports, 2019-11, Vol.9 (1), p.17131-7, Article 17131</ispartof><rights>The Author(s) 2019</rights><rights>2019. 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An optimized fluorescence microscopic add-on system for a smartphone was used to image the 300 nm fluorescent beads. Sequential nanobead images were recorded for a period and the image data were used for fluorescence correlation spectrometric (FCS) analysis. Through the analysis, the nanobeads’ diffusion coefficient was obtained. Further, the diffusion coefficients of the anti-CRP-coated nanobeads, which were suspended in the samples with various CRP concentrations, were estimated using smartphone-based diffusometry. After 10 min of reaction, the anti-CRP-coated nanobeads in a higher CRP concentration solution led to a lower diffusion coefficient. 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Deng, Chih-Zong ; Fang, Yi-Fan ; Jiang, Hong-Ren ; Tseng, Pao-Wei ; Sheen, Horn-Jiunn ; Fan, Yu-Jui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-4db7bfe49b8e031a2c416c63958ddceb01774827e6345328aa2e217de97d2bed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>631/1647/2196/2197</topic><topic>692/700</topic><topic>9/10</topic><topic>96/63</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensing Techniques - methods</topic><topic>C-reactive protein</topic><topic>C-Reactive Protein - chemistry</topic><topic>Diffusion coefficient</topic><topic>Fluorescence</topic><topic>Humanities and Social Sciences</topic><topic>Immunoassay - instrumentation</topic><topic>Immunoassay - methods</topic><topic>Immunologic Tests - instrumentation</topic><topic>Immunologic Tests - methods</topic><topic>multidisciplinary</topic><topic>Nanoparticles - chemistry</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Smartphone</topic><topic>Smartphones</topic><topic>Spectrometry, Fluorescence - instrumentation</topic><topic>Spectrometry, Fluorescence - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chuang, Chih-Shen</creatorcontrib><creatorcontrib>Deng, Chih-Zong</creatorcontrib><creatorcontrib>Fang, Yi-Fan</creatorcontrib><creatorcontrib>Jiang, Hong-Ren</creatorcontrib><creatorcontrib>Tseng, Pao-Wei</creatorcontrib><creatorcontrib>Sheen, Horn-Jiunn</creatorcontrib><creatorcontrib>Fan, Yu-Jui</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chuang, Chih-Shen</au><au>Deng, Chih-Zong</au><au>Fang, Yi-Fan</au><au>Jiang, Hong-Ren</au><au>Tseng, Pao-Wei</au><au>Sheen, Horn-Jiunn</au><au>Fan, Yu-Jui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Smartphone-based Diffusometric Immunoassay for Detecting C-Reactive Protein</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2019-11-20</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>17131</spage><epage>7</epage><pages>17131-7</pages><artnum>17131</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>In this study, we developed a portable smartphone-based diffusometry for analyzing the C-reactive protein (CRP) concentration. An optimized fluorescence microscopic add-on system for a smartphone was used to image the 300 nm fluorescent beads. Sequential nanobead images were recorded for a period and the image data were used for fluorescence correlation spectrometric (FCS) analysis. Through the analysis, the nanobeads’ diffusion coefficient was obtained. Further, the diffusion coefficients of the anti-CRP-coated nanobeads, which were suspended in the samples with various CRP concentrations, were estimated using smartphone-based diffusometry. After 10 min of reaction, the anti-CRP-coated nanobeads in a higher CRP concentration solution led to a lower diffusion coefficient. Based on the experiments, a linear sensing range of 1~8 µg/mL was found.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31748592</pmid><doi>10.1038/s41598-019-52285-4</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 631/1647/2196/2197 692/700 9/10 96/63 Biosensing Techniques - instrumentation Biosensing Techniques - methods C-reactive protein C-Reactive Protein - chemistry Diffusion coefficient Fluorescence Humanities and Social Sciences Immunoassay - instrumentation Immunoassay - methods Immunologic Tests - instrumentation Immunologic Tests - methods multidisciplinary Nanoparticles - chemistry Science Science (multidisciplinary) Smartphone Smartphones Spectrometry, Fluorescence - instrumentation Spectrometry, Fluorescence - methods |
| title | A Smartphone-based Diffusometric Immunoassay for Detecting C-Reactive Protein |
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