Genotypes II and VIId-based inactivated Newcastle disease vaccine reduces virus shedding
In Egypt, recent outbreaks were reported in NDV-vaccinated flocks. The isolated strain was characterized as class II velogenic genotype VIId of Newcastle disease virus (NDV). In this study, three inactivated NDV vaccine formulations were prepared, the first one is LaSota (genotype II), the second on...
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| Veröffentlicht in: | VirusDisease 2019-09, Vol.30 (3), p.453-461 |
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| creator | Mahmoud, Nehal K. El-Deeb, Ayman H. Emara, Mohammed M. Abd El-Khaleck, M. A. Hussein, Hussein A. |
| description | In Egypt, recent outbreaks were reported in NDV-vaccinated flocks. The isolated strain was characterized as class II velogenic genotype VIId of Newcastle disease virus (NDV). In this study, three inactivated NDV vaccine formulations were prepared, the first one is LaSota (genotype II), the second one is genotype VIId and the third one is combined Lasota and genotype VIId at a proportion of 1:1. The challenge trials were conducted in SPF chicks to evaluate the efficacy of the prepared vaccines using 10
6
EID
50
/0.5 ml of the Egyptian genotype VIId strain of Newcastle disease virus (NDV-B7-RLQP-CH-EG-12). Our results revealed that all three prepared vaccine formulations conferred 100% protection in the vaccinated chicks. However, the combined vaccine induced the highest haemagglutination inhibition (HI) titers and neutralization indices with significant reduction in virus shedding compared to other vaccine formulations. Histopathology examination of different organs collected from vaccinated chicks post challenge indicated the protective efficacy in vaccinated groups compared to the positive control group where a score of severe lesions was shown. This study reports the efficacy of combined inactivated Lasota and genotype VIId vaccine in reducing virus shedding which is very important in controlling NDV infection in chicken. |
| doi_str_mv | 10.1007/s13337-019-00537-2 |
| format | Article |
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6
EID
50
/0.5 ml of the Egyptian genotype VIId strain of Newcastle disease virus (NDV-B7-RLQP-CH-EG-12). Our results revealed that all three prepared vaccine formulations conferred 100% protection in the vaccinated chicks. However, the combined vaccine induced the highest haemagglutination inhibition (HI) titers and neutralization indices with significant reduction in virus shedding compared to other vaccine formulations. Histopathology examination of different organs collected from vaccinated chicks post challenge indicated the protective efficacy in vaccinated groups compared to the positive control group where a score of severe lesions was shown. This study reports the efficacy of combined inactivated Lasota and genotype VIId vaccine in reducing virus shedding which is very important in controlling NDV infection in chicken.</description><identifier>ISSN: 2347-3584</identifier><identifier>EISSN: 2347-3517</identifier><identifier>DOI: 10.1007/s13337-019-00537-2</identifier><identifier>PMID: 31803813</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>Antigens ; Biochemistry ; Biomedical and Life Sciences ; Cell Biology ; Combined vaccines ; Gene amplification ; Genetic aspects ; Genotype & phenotype ; Genotypes ; Hemagglutination inhibition ; Immunization ; Infections ; Juveniles ; Life Sciences ; Medical research ; Medicine, Experimental ; Microbiology ; Newcastle disease ; Original ; Original Article ; Pathogens ; Poultry ; Protein Structure ; Serology ; Vaccination ; Vaccines ; Variance analysis ; Virulence ; Viruses</subject><ispartof>VirusDisease, 2019-09, Vol.30 (3), p.453-461</ispartof><rights>Indian Virological Society 2019</rights><rights>Indian Virological Society 2019.</rights><rights>COPYRIGHT 2019 Springer</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4562-87e3db620abaa219d91975bbe38381e7f9aae006eadda70401e7c778566423f33</citedby><cites>FETCH-LOGICAL-c4562-87e3db620abaa219d91975bbe38381e7f9aae006eadda70401e7c778566423f33</cites><orcidid>0000-0002-5589-1985</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6864008/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2919619807?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>230,315,729,782,786,887,21395,21396,27931,27932,33537,33538,33751,33752,41495,42564,43666,43812,51326,53798,53800,64392,64394,64396,72476</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31803813$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mahmoud, Nehal K.</creatorcontrib><creatorcontrib>El-Deeb, Ayman H.</creatorcontrib><creatorcontrib>Emara, Mohammed M.</creatorcontrib><creatorcontrib>Abd El-Khaleck, M. A.</creatorcontrib><creatorcontrib>Hussein, Hussein A.</creatorcontrib><title>Genotypes II and VIId-based inactivated Newcastle disease vaccine reduces virus shedding</title><title>VirusDisease</title><addtitle>VirusDis</addtitle><addtitle>Virusdisease</addtitle><description>In Egypt, recent outbreaks were reported in NDV-vaccinated flocks. The isolated strain was characterized as class II velogenic genotype VIId of Newcastle disease virus (NDV). In this study, three inactivated NDV vaccine formulations were prepared, the first one is LaSota (genotype II), the second one is genotype VIId and the third one is combined Lasota and genotype VIId at a proportion of 1:1. The challenge trials were conducted in SPF chicks to evaluate the efficacy of the prepared vaccines using 10
6
EID
50
/0.5 ml of the Egyptian genotype VIId strain of Newcastle disease virus (NDV-B7-RLQP-CH-EG-12). Our results revealed that all three prepared vaccine formulations conferred 100% protection in the vaccinated chicks. However, the combined vaccine induced the highest haemagglutination inhibition (HI) titers and neutralization indices with significant reduction in virus shedding compared to other vaccine formulations. Histopathology examination of different organs collected from vaccinated chicks post challenge indicated the protective efficacy in vaccinated groups compared to the positive control group where a score of severe lesions was shown. This study reports the efficacy of combined inactivated Lasota and genotype VIId vaccine in reducing virus shedding which is very important in controlling NDV infection in chicken.</description><subject>Antigens</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Combined vaccines</subject><subject>Gene amplification</subject><subject>Genetic aspects</subject><subject>Genotype & phenotype</subject><subject>Genotypes</subject><subject>Hemagglutination inhibition</subject><subject>Immunization</subject><subject>Infections</subject><subject>Juveniles</subject><subject>Life Sciences</subject><subject>Medical research</subject><subject>Medicine, Experimental</subject><subject>Microbiology</subject><subject>Newcastle disease</subject><subject>Original</subject><subject>Original Article</subject><subject>Pathogens</subject><subject>Poultry</subject><subject>Protein Structure</subject><subject>Serology</subject><subject>Vaccination</subject><subject>Vaccines</subject><subject>Variance analysis</subject><subject>Virulence</subject><subject>Viruses</subject><issn>2347-3584</issn><issn>2347-3517</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9UcFu1TAQtBCIVqU_wAFF4sIlZW0ndnJBqiookapyAcTN2tibV1d5zsNOHurf45LygB6QD17tzszOahh7yeGMA-i3iUspdQm8LQHqXIkn7FjISpey5vrpoW6qI3aa0i0AcK6rSrXP2ZHkDciGy2P27ZLCNN_tKBVdV2Bwxdeuc2WPiVzhA9rZ73HO9TX9sJjmkQrnE-VxsUdrfaAiklts5u99XFKRbsg5HzYv2LMBx0SnD_8J-_Lh_eeLj-XVp8vu4vyqtFWtRNlokq5XArBHFLx1LW913fckm2yQ9NAiEoAidA41VJB7VuumVqoScpDyhL1bdXdLvyVnKcwRR7OLfovxzkzozb-T4G_MZtob1agKoMkCbx4E4vR9oTSbrU-WxhEDTUsyQgrBKw5CZOjrR9DbaYkhn2dE9q1424DOqLMVtcGRjA_DlPfa_BxtvZ0CDT73zzXnSte65pkgVoKNU0qRhoN7DuY-bLOGbXLY5lfY5t7Lq7_vPlB-R5sBcgWkPAobin_M_kf2J0-HtC8</recordid><startdate>20190901</startdate><enddate>20190901</enddate><creator>Mahmoud, Nehal K.</creator><creator>El-Deeb, Ayman H.</creator><creator>Emara, Mohammed M.</creator><creator>Abd El-Khaleck, M. 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A. ; Hussein, Hussein A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4562-87e3db620abaa219d91975bbe38381e7f9aae006eadda70401e7c778566423f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antigens</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>Combined vaccines</topic><topic>Gene amplification</topic><topic>Genetic aspects</topic><topic>Genotype & phenotype</topic><topic>Genotypes</topic><topic>Hemagglutination inhibition</topic><topic>Immunization</topic><topic>Infections</topic><topic>Juveniles</topic><topic>Life Sciences</topic><topic>Medical research</topic><topic>Medicine, Experimental</topic><topic>Microbiology</topic><topic>Newcastle disease</topic><topic>Original</topic><topic>Original Article</topic><topic>Pathogens</topic><topic>Poultry</topic><topic>Protein Structure</topic><topic>Serology</topic><topic>Vaccination</topic><topic>Vaccines</topic><topic>Variance analysis</topic><topic>Virulence</topic><topic>Viruses</topic><toplevel>online_resources</toplevel><creatorcontrib>Mahmoud, Nehal K.</creatorcontrib><creatorcontrib>El-Deeb, Ayman H.</creatorcontrib><creatorcontrib>Emara, Mohammed M.</creatorcontrib><creatorcontrib>Abd El-Khaleck, M. 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A.</au><au>Hussein, Hussein A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genotypes II and VIId-based inactivated Newcastle disease vaccine reduces virus shedding</atitle><jtitle>VirusDisease</jtitle><stitle>VirusDis</stitle><addtitle>Virusdisease</addtitle><date>2019-09-01</date><risdate>2019</risdate><volume>30</volume><issue>3</issue><spage>453</spage><epage>461</epage><pages>453-461</pages><issn>2347-3584</issn><eissn>2347-3517</eissn><abstract>In Egypt, recent outbreaks were reported in NDV-vaccinated flocks. The isolated strain was characterized as class II velogenic genotype VIId of Newcastle disease virus (NDV). In this study, three inactivated NDV vaccine formulations were prepared, the first one is LaSota (genotype II), the second one is genotype VIId and the third one is combined Lasota and genotype VIId at a proportion of 1:1. The challenge trials were conducted in SPF chicks to evaluate the efficacy of the prepared vaccines using 10
6
EID
50
/0.5 ml of the Egyptian genotype VIId strain of Newcastle disease virus (NDV-B7-RLQP-CH-EG-12). Our results revealed that all three prepared vaccine formulations conferred 100% protection in the vaccinated chicks. However, the combined vaccine induced the highest haemagglutination inhibition (HI) titers and neutralization indices with significant reduction in virus shedding compared to other vaccine formulations. Histopathology examination of different organs collected from vaccinated chicks post challenge indicated the protective efficacy in vaccinated groups compared to the positive control group where a score of severe lesions was shown. This study reports the efficacy of combined inactivated Lasota and genotype VIId vaccine in reducing virus shedding which is very important in controlling NDV infection in chicken.</abstract><cop>New Delhi</cop><pub>Springer India</pub><pmid>31803813</pmid><doi>10.1007/s13337-019-00537-2</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-5589-1985</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Antigens Biochemistry Biomedical and Life Sciences Cell Biology Combined vaccines Gene amplification Genetic aspects Genotype & phenotype Genotypes Hemagglutination inhibition Immunization Infections Juveniles Life Sciences Medical research Medicine, Experimental Microbiology Newcastle disease Original Original Article Pathogens Poultry Protein Structure Serology Vaccination Vaccines Variance analysis Virulence Viruses |
| title | Genotypes II and VIId-based inactivated Newcastle disease vaccine reduces virus shedding |
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