Integrative analysis of pooled CRISPR genetic screens using MAGeCKFlute

Genome-wide screening using CRISPR coupled with nuclease Cas9 (CRISPR–Cas9) is a powerful technology for the systematic evaluation of gene function. Statistically principled analysis is needed for the accurate identification of gene hits and associated pathways. Here, we describe how to perform comp...

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Veröffentlicht in:	Nature protocols 2019-03, Vol.14 (3), p.756-780
Hauptverfasser:	Wang, Binbin, Wang, Mei, Zhang, Wubing, Xiao, Tengfei, Chen, Chen-Hao, Wu, Alexander, Wu, Feizhen, Traugh, Nicole, Wang, Xiaoqing, Li, Ziyi, Mei, Shenglin, Cui, Yingbo, Shi, Sailing, Lipp, Jesse Jonathan, Hinterndorfer, Matthias, Zuber, Johannes, Brown, Myles, Li, Wei, Liu, X. Shirley
Format:	Artikel
Sprache:	eng
Schlagworte:	631/114 631/114/2163 631/1647/2163 Algorithms Analytical Chemistry Biological Techniques Biomedical and Life Sciences Computational Biology/Bioinformatics Computer applications CRISPR CRISPR-Associated Protein 9 - metabolism CRISPR-Cas systems CRISPR-Cas Systems - genetics Data analysis Data processing Gene Dosage Genes, Essential Genetic analysis Genetic screening Genetic testing Genetic Testing - methods Genome Genomes Glioblastoma - genetics Glioblastoma - pathology Humans Life Sciences Methods Microarrays Neoplastic Stem Cells - pathology Nuclease Organic Chemistry Personal computers Pipelines Protocol Quality control Technology assessment
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