Ultrasound imaging of gene expression in mammalian cells
The study of cellular processes occurring inside intact organisms requires methods to visualize cellular functions such as gene expression in deep tissues. Ultrasound is a widely used biomedical technology enabling noninvasive imaging with high spatial and temporal resolution. However, no geneticall...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2019-09, Vol.365 (6460), p.1469-1475 |
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| container_title | Science (American Association for the Advancement of Science) |
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| creator | Farhadi, Arash Ho, Gabrielle H. Sawyer, Daniel P. Bourdeau, Raymond W. Shapiro, Mikhail G. |
| description | The study of cellular processes occurring inside intact organisms requires methods to visualize cellular functions such as gene expression in deep tissues. Ultrasound is a widely used biomedical technology enabling noninvasive imaging with high spatial and temporal resolution. However, no genetically encoded molecular reporters are available to connect ultrasound contrast to gene expression in mammalian cells. To address this limitation, we introduce mammalian acoustic reporter genes. Starting with a gene cluster derived from bacteria, we engineered a eukaryotic genetic program whose introduction into mammalian cells results in the expression of intracellular air-filled protein nanostructures called gas vesicles, which produce ultrasound contrast. Mammalian acoustic reporter genes allow cells to be visualized at volumetric densities below 0.5% and permit high-resolution imaging of gene expression in living animals. |
| doi_str_mv | 10.1126/science.aax4804 |
| format | Article |
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Ultrasound is a widely used biomedical technology enabling noninvasive imaging with high spatial and temporal resolution. However, no genetically encoded molecular reporters are available to connect ultrasound contrast to gene expression in mammalian cells. To address this limitation, we introduce mammalian acoustic reporter genes. Starting with a gene cluster derived from bacteria, we engineered a eukaryotic genetic program whose introduction into mammalian cells results in the expression of intracellular air-filled protein nanostructures called gas vesicles, which produce ultrasound contrast. Mammalian acoustic reporter genes allow cells to be visualized at volumetric densities below 0.5% and permit high-resolution imaging of gene expression in living animals.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.aax4804</identifier><identifier>PMID: 31604277</identifier><language>eng</language><publisher>United States: American Association for the Advancement of Science</publisher><subject>Acoustics ; Anabaena flos-aquae - genetics ; Animals ; Bacillus megaterium - genetics ; Bacteria ; CHO Cells ; Cricetulus ; Gene Expression ; Genes ; Genes, Reporter ; Genetic code ; Halobacterium salinarum - genetics ; HEK293 Cells ; Humans ; Image resolution ; Mammalian cells ; Mammals ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Multigene Family ; Nanostructures - chemistry ; Proteins ; Proteins - genetics ; Sound waves ; Temporal resolution ; Tissues ; Transfection ; Ultrasonic imaging ; Ultrasonography ; Ultrasound ; Xenografts ; Xenotransplantation</subject><ispartof>Science (American Association for the Advancement of Science), 2019-09, Vol.365 (6460), p.1469-1475</ispartof><rights>Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.</rights><rights>Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c546t-3d85c99b21b4fc88f9c3e4674d67785fbb722dd569495954f5ca47896626dc5a3</citedby><cites>FETCH-LOGICAL-c546t-3d85c99b21b4fc88f9c3e4674d67785fbb722dd569495954f5ca47896626dc5a3</cites><orcidid>0000-0003-2202-1980 ; 0000-0002-0291-4215 ; 0000-0001-9137-8559 ; 0000-0003-2926-191X ; 0000-0002-8511-5549</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,2871,2872,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31604277$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Farhadi, Arash</creatorcontrib><creatorcontrib>Ho, Gabrielle H.</creatorcontrib><creatorcontrib>Sawyer, Daniel P.</creatorcontrib><creatorcontrib>Bourdeau, Raymond W.</creatorcontrib><creatorcontrib>Shapiro, Mikhail G.</creatorcontrib><title>Ultrasound imaging of gene expression in mammalian cells</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>The study of cellular processes occurring inside intact organisms requires methods to visualize cellular functions such as gene expression in deep tissues. Ultrasound is a widely used biomedical technology enabling noninvasive imaging with high spatial and temporal resolution. However, no genetically encoded molecular reporters are available to connect ultrasound contrast to gene expression in mammalian cells. To address this limitation, we introduce mammalian acoustic reporter genes. Starting with a gene cluster derived from bacteria, we engineered a eukaryotic genetic program whose introduction into mammalian cells results in the expression of intracellular air-filled protein nanostructures called gas vesicles, which produce ultrasound contrast. 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| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 2019-09, Vol.365 (6460), p.1469-1475 |
| issn | 0036-8075 1095-9203 |
| language | eng |
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| source | American Association for the Advancement of Science; MEDLINE |
| subjects | Acoustics Anabaena flos-aquae - genetics Animals Bacillus megaterium - genetics Bacteria CHO Cells Cricetulus Gene Expression Genes Genes, Reporter Genetic code Halobacterium salinarum - genetics HEK293 Cells Humans Image resolution Mammalian cells Mammals Mice Mice, Inbred NOD Mice, SCID Multigene Family Nanostructures - chemistry Proteins Proteins - genetics Sound waves Temporal resolution Tissues Transfection Ultrasonic imaging Ultrasonography Ultrasound Xenografts Xenotransplantation |
| title | Ultrasound imaging of gene expression in mammalian cells |
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