Kinetic and subcellular analysis of PS-ASO/protein interactions with P54nrb and RNase H1

Abstract The rapid RNase H1-dependent mislocalization of heterodimer proteins P54nrb and PSF to nucleoli is an early event in the pathway that explains the effects of most toxic phosphorothioate ASOs (PS-ASOs). Using a recently developed NanoLuciferace (NLuc)-based structural complementation reporte...

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Veröffentlicht in:	Nucleic acids research 2019-11, Vol.47 (20), p.10865-10880
Hauptverfasser:	Vickers, Timothy A, Rahdar, Meghdad, Prakash, Thazha P, Crooke, Stanley T
Format:	Artikel
Sprache:	eng
Schlagworte:	Catalytic Domain Cell Nucleolus - metabolism Cell Survival DNA-Binding Proteins - chemistry DNA-Binding Proteins - metabolism HEK293 Cells HeLa Cells Humans Kinetics Oligonucleotides, Antisense - metabolism Phosphorothioate Oligonucleotides - metabolism Protein Binding Ribonuclease H - chemistry Ribonuclease H - metabolism RNA and RNA-protein complexes RNA-Binding Proteins - chemistry RNA-Binding Proteins - metabolism
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